scholarly journals Molecular Confirmation of Massive Taenia pisiformis Cysticercosis in One Rabbit in Poland

Pathogens ◽  
2021 ◽  
Vol 10 (8) ◽  
pp. 1029
Author(s):  
Małgorzata Samorek-Pieróg ◽  
Jacek Karamon ◽  
Adam Brzana ◽  
Ewa Bilska-Zając ◽  
Jolanta Zdybel ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Cytochrome Oxidase ◽  
Molecular Characterization ◽  
Molecular Analysis ◽  
Ribosomal Rna ◽  
Nadh Dehydrogenase ◽  
12S Rrna ◽  
Nadh Dehydrogenase Subunit ◽  
Cytochrome Oxidase Subunit ◽  
Cytochrome Oxidase Subunit 1

The aim of this study was to provide molecular characterization, together with phylogenetic analysis, of Taenia pisiformis cysts isolated from rabbit. On the basis of morphological features and molecular analysis, the cysticerci were identified as T.pisiformis metacestodes. PCR was performed with three different protocols to obtain partial sequences of 12S ribosomal RNA (12S rRNA), NADH dehydrogenase subunit 1 (nad1), and cytochrome oxidase subunit 1 (cox1) of Taenia spp. The products from the PCRs were sequenced. Interpretation of the sequencing results of the obtained amplicons, by comparing them with the GenBank database, proved that the causative agent, in this case, was T. pisiformis. The phylogenetic analysis of the received sequences identified a new haplotype. The received data can be used to supplement the species description. To our knowledge, this is the first molecular confirmation of T. pisiformis metacestodes infection in the rabbit, in Poland.

scholarly journals Sequence Analysis of Mitochondrial DNAs of 12S rRNA, 16S rRNA, and Cytochrome Oxidase Subunit 1(COI) Regions in Slow Lorises (Genus Nycticebus) May Contribute to Improved Identification of Confiscated Specimens

ISRN Zoology ◽  
2012 ◽  
Vol 2012 ◽  
pp. 1-8 ◽  
Author(s):  
Hiroko Somura ◽  
Hiroshi Hori ◽  
Yoshinobu Manome
Keyword(s):  
16S Rrna ◽  
Cytochrome Oxidase ◽  
Dna Analysis ◽  
12S Rrna ◽  
Cytochrome Oxidase Subunit ◽  
Slow Loris ◽  
Oxidase Subunit ◽  
Mitochondrial Dnas ◽  
Cytochrome Oxidase Subunit 1 ◽  
Rrna 16S

The slow loris (Nycticebus) is a prosimian that is popular among exotic pet lovers. In Japan, many slow lorises have been imported illegally. Prosimians that have been confiscated in raids are protected in Japanese zoos, and the number of such animals has increased. In most cases, the country of origin remains unknown and even the species can be difficult to identify from the animal’s physical appearance alone. We have attempted to resolve this problem by using DNA analysis. DNA samples of five species, consisting of the Pygmy slow loris (Nycticebus pygmaeus), Bengal slow loris (Nycticebus bengalensis), Sunda slow loris (Nycticebus coucang), Javan slow loris (Nycticebus javanicus), and Bornean slow loris (Nycticebus menagensis), were extracted, amplified, and the nucleotide sequences of mitochondrial 12S rRNA, 16S rRNA, and the cytochrome oxidase subunit 1(COI) regions were compared. Differences of nucleic acid sequences of representative individuals were demonstrated.

Phylogenetic analysis of the phoretic association between Bursaphelenchus conicaudatus (Nematoda: Aphelenchoididae) and Psacothea hilaris (Coleoptera: Cerambycidae)

Nematology ◽  
2002 ◽  
Vol 4 (6) ◽  
pp. 759-771 ◽  
Author(s):  
Kazuyoshi Futai ◽  
Natsumi Kanzaki
Keyword(s):  
Phylogenetic Analysis ◽  
Mitochondrial Dna ◽  
Cytochrome Oxidase ◽  
Molecular Analysis ◽  
Phylogenetic Relationships ◽  
Base Sequence ◽  
Cytochrome Oxidase Subunit ◽  
Cytochrome Oxidase Subunit I ◽  
Oxidase Subunit ◽  
Longicorn Beetle

AbstractThe phoretic relationships between the nematode Bursaphelenchus conicaudatus and the yellow-spotted longicorn beetle, Psacothea hilaris, collected at various districts in Japan were investigated. A high proportion of all ten subspecies of the beetle examined were infected with the nematodes, suggesting the universality of this phoretic association. Molecular analysis based on the partial base sequence of cytochrome oxidase subunit I in the mitochondrial DNA revealed that the phylogenetic relationships among ten subspecies of the beetle was similar to that among associated nematode isolates obtained from each beetle subspecies. Intraspecific vector replacements were supposed to occur in the isolates of B. conicaudatus on two islands. The phoretic association between the nematode and the beetle is assumed to have been established before the divergence of P.hilaris into subspecies.

scholarly journals Diversity and Molecular Characterization of Mosquitoes (Diptera: Culicidae) in Selected Ecological Regions in Kenya.

F1000Research ◽  
2019 ◽  
Vol 8 ◽  
pp. 262
Author(s):  
Moni Makanda ◽  
Gladys Kemunto ◽  
Lucy Wamuyu ◽  
Joel Bargul ◽  
Jackson Muema ◽  
...  
Keyword(s):  
Cytochrome Oxidase ◽  
Molecular Characterization ◽  
Mosquito Species ◽  
Molecular Genetic ◽  
Cytochrome Oxidase Subunit ◽  
Valley Fever ◽  
Genetic Characteristics ◽  
Oxidase Subunit ◽  
Cytochrome Oxidase Subunit 1

Mosquitoes play a predominant role as leading agents in the spread of vector-borne diseases and the consequent mortality in humans. Despite reports on increase of new and recurrent mosquito borne-disease outbreaks such as chikungunya, dengue fever and Rift Valley fever in Kenya, little is known about the genetic characteristics and diversity of the vector species that have been incriminated in transmission of disease pathogens. In this study,  mosquito species were collected from Kisumu city, Kilifi town and Nairobi city and we determined their genetic diversity and phylogenetic relationships. PCR was used to amplify the partial cytochrome oxidase subunit 1 (CO1) gene of mosquito samples. Molecular-genetic and phylogenetic analysis of the partial cytochrome oxidase subunit 1 (CO1) gene were employed to identify their relationship with known mosquito species. Fourteen (14) haplotypes belonging to genus Aedes, nine (9) haplotypes belonging to genus Anopheles and twelve (12) haplotypes belonging to genus Culex were identified in this study. Findings from this study revealed a potentially new haplotype belonging to Anopheles genus and reported the first molecular characterization of Aedes cumminsii in Kenya. Sequence results revealed variation in mosquito species from Kilifi, Kisumu and Nairobi. Since vector competence varies greatly across species as well as species-complexes and is strongly associated with specific behavioural adaptations, proper species identification is important for vector control programs.

scholarly journals Diversity and Molecular Characterization of Mosquitoes (Diptera: Culicidae) in selected ecological regions in Kenya.

F1000Research ◽  
2019 ◽  
Vol 8 ◽  
pp. 262 ◽  
Author(s):  
Moni Makanda ◽  
Gladys Kemunto ◽  
Lucy Wamuyu ◽  
Joel Bargul ◽  
Jackson Muema ◽  
...  
Keyword(s):  
Cytochrome Oxidase ◽  
Molecular Characterization ◽  
Mosquito Species ◽  
Molecular Genetic ◽  
Cytochrome Oxidase Subunit ◽  
Valley Fever ◽  
Genetic Characteristics ◽  
Oxidase Subunit ◽  
Cytochrome Oxidase Subunit 1

Mosquitoes play a predominant role as leading agents in the spread of vector-borne diseases and consequent mortality in humans. Despite reports on increase of new and recurrent mosquito borne-disease outbreaks such as chikungunya, dengue fever and Rift valley fever in Kenya little is known about the genetic characteristics and diversity of the vector species that have been incriminated in transmission of disease pathogens. In this study, we identified mosquito species across Kisumu, Kilifi and Nairobi Counties and determined their genetic diversity and phylogenetic relationships. PCR was used to amplify and sequence the partial cytochrome oxidase subunit 1 (CO1) gene of mosquito samples. Molecular-genetic and phylogenetic analysis of the partial cytochrome oxidase subunit 1 (CO1) gene was employed to identify their relationships with known mosquito species. Fourteen (14) haplotypes belonging to genus Aedes, nine (9) haplotypes belonging to genus Anopheles and twelve (12) haplotypes belonging to genus Culex were identified in this study. Findings from this study revealed a potentially new haplotype belonging to Anopheles genus and reported the first molecular characterization of Aedes cummnisii in Kenya. Sequence results revealed variation in mosquito species from Kilifi, Kisumu and Nairobi. Since vector competence varies greatly across species and species-complexes and is strongly associated with specific behavioural adaptations, proper species identification is important for vector control programs.

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