scholarly journals Phenylpropanoid Pathway Engineering: An Emerging Approach towards Plant Defense

Pathogens ◽  
2020 ◽  
Vol 9 (4) ◽  
pp. 312 ◽  
Author(s):  
Vivek Yadav ◽  
Zhongyuan Wang ◽  
Chunhua Wei ◽  
Aduragbemi Amo ◽  
Bilal Ahmed ◽  
...  
Keyword(s):  
Cell Wall ◽  
Plant Defense ◽  
Plant Cell Wall ◽  
Shikimate Pathway ◽  
Gene Families ◽  
Phenylpropanoid Pathway ◽  
Pathway Engineering ◽  
Multiple Gene ◽  
Regulator Genes ◽  
Microbial Attack

Pathogens hitting the plant cell wall is the first impetus that triggers the phenylpropanoid pathway for plant defense. The phenylpropanoid pathway bifurcates into the production of an enormous array of compounds based on the few intermediates of the shikimate pathway in response to cell wall breaches by pathogens. The whole metabolomic pathway is a complex network regulated by multiple gene families and it exhibits refined regulatory mechanisms at the transcriptional, post-transcriptional, and post-translational levels. The pathway genes are involved in the production of anti-microbial compounds as well as signaling molecules. The engineering in the metabolic pathway has led to a new plant defense system of which various mechanisms have been proposed including salicylic acid and antimicrobial mediated compounds. In recent years, some key players like phenylalanine ammonia lyases (PALs) from the phenylpropanoid pathway are proposed to have broad spectrum disease resistance (BSR) without yield penalties. Now we have more evidence than ever, yet little understanding about the pathway-based genes that orchestrate rapid, coordinated induction of phenylpropanoid defenses in response to microbial attack. It is not astonishing that mutants of pathway regulator genes can show conflicting results. Therefore, precise engineering of the pathway is an interesting strategy to aim at profitably tailored plants. Here, this review portrays the current progress and challenges for phenylpropanoid pathway-based resistance from the current prospective to provide a deeper understanding.

scholarly journals Direct evidence for a new mode of plant defense against insects via a novel polygalacturonase-inhibiting protein expression strategy

2020 ◽  
Vol 295 (33) ◽  
pp. 11833-11844
Author(s):  
Wiebke Haeger ◽  
Jana Henning ◽  
David G. Heckel ◽  
Yannick Pauchet ◽  
Roy Kirsch
Keyword(s):  
Cell Wall ◽  
Plant Defense ◽  
Plant Cell Wall ◽  
Direct Evidence ◽  
Larval Growth ◽  
Gene Families ◽  
Preliminary Evidence ◽  
Cell Wall Polysaccharide ◽  
In Planta

Plant cell wall–associated polygalacturonase-inhibiting proteins (PGIPs) are widely distributed in the plant kingdom. They play a crucial role in plant defense against phytopathogens by inhibiting microbial polygalacturonases (PGs). PGs hydrolyze the cell wall polysaccharide pectin and are among the first enzymes to be secreted during plant infection. Recent studies demonstrated that herbivorous insects express their own PG multi-gene families, raising the question whether PGIPs also inhibit insect PGs and protect plants from herbivores. Preliminary evidence suggested that PGIPs may negatively influence larval growth of the leaf beetle Phaedon cochleariae (Coleoptera: Chrysomelidae) and identified BrPGIP3 from Chinese cabbage (Brassica rapa ssp. pekinensis) as a candidate. PGIPs are predominantly studied in planta because their heterologous expression in microbial systems is problematic and instability and aggregation of recombinant PGIPs has complicated in vitro inhibition assays. To minimize aggregate formation, we heterologously expressed BrPGIP3 fused to a glycosylphosphatidylinositol (GPI) membrane anchor, immobilizing it on the extracellular surface of insect cells. We demonstrated that BrPGIP3_GPI inhibited several P. cochleariae PGs in vitro, providing the first direct evidence of an interaction between a plant PGIP and an animal PG. Thus, plant PGIPs not only confer resistance against phytopathogens, but may also aid in defense against herbivorous beetles.

scholarly journals The Role of Arabinogalactan Type II Degradation in Plant-Microbe Interactions

2021 ◽  
Vol 12 ◽  
Author(s):  
Maria Guadalupe Villa-Rivera ◽  
Horacio Cano-Camacho ◽  
Everardo López-Romero ◽  
María Guadalupe Zavala-Páramo
Keyword(s):  
Cell Wall ◽  
Plant Defense ◽  
Plant Cell ◽  
Plant Cell Wall ◽  
Degradation Products ◽  
Hydrolytic Enzymes ◽  
Pathogenic Fungi ◽  
Plant Interactions ◽  
Root Tips ◽  
Microbe Interactions

Arabinogalactans (AGs) are structural polysaccharides of the plant cell wall. A small proportion of the AGs are associated with hemicellulose and pectin. Furthermore, AGs are associated with proteins forming the so-called arabinogalactan proteins (AGPs), which can be found in the plant cell wall or attached through a glycosylphosphatidylinositol (GPI) anchor to the plasma membrane. AGPs are a family of highly glycosylated proteins grouped with cell wall proteins rich in hydroxyproline. These glycoproteins have important and diverse functions in plants, such as growth, cellular differentiation, signaling, and microbe-plant interactions, and several reports suggest that carbohydrate components are crucial for AGP functions. In beneficial plant-microbe interactions, AGPs attract symbiotic species of fungi or bacteria, promote the development of infectious structures and the colonization of root tips, and furthermore, these interactions can activate plant defense mechanisms. On the other hand, plants secrete and accumulate AGPs at infection sites, creating cross-links with pectin. As part of the plant cell wall degradation machinery, beneficial and pathogenic fungi and bacteria can produce the enzymes necessary for the complete depolymerization of AGs including endo-β-(1,3), β-(1,4) and β-(1,6)-galactanases, β-(1,3/1,6) galactanases, α-L-arabinofuranosidases, β-L-arabinopyranosidases, and β-D-glucuronidases. These hydrolytic enzymes are secreted during plant-pathogen interactions and could have implications for the function of AGPs. It has been proposed that AGPs could prevent infection by pathogenic microorganisms because their degradation products generated by hydrolytic enzymes of pathogens function as damage-associated molecular patterns (DAMPs) eliciting the plant defense response. In this review, we describe the structure and function of AGs and AGPs as components of the plant cell wall. Additionally, we describe the set of enzymes secreted by microorganisms to degrade AGs from AGPs and its possible implication for plant-microbe interactions.

scholarly journals Identification of Putative Cell Wall Synthesis Genes in Betula pendula

2020 ◽  
Author(s):  
Song Chen ◽  
Xin Lin ◽  
Xiyang Zhao ◽  
Su Chen
Keyword(s):  
Cell Wall ◽  
Betula Pendula ◽  
Secondary Cell Wall ◽  
Plant Cell Wall ◽  
Gene Families ◽  
Cellulose Synthase ◽  
Wall Structure ◽  
Cellulose Synthesis ◽  
Cell Wall Synthesis ◽  
Secondary Cell Wall Synthesis

Abstract BackgroundCellulose is an essential structural component of plant cell wall and is an important resource to produce paper, textiles, bioplastics and other biomaterials. The synthesis of cellulose is among the most important but poorly understood biochemical processes, which is precisely regulated by internal and external cues.ResultsHere, we identified 46 gene models in 7 gene families which encoding cellulose synthase and related enzymes of Betula pendula, and the transcript abundance of these genes in xylem, root, leaf and flower tissues also be determined. Based on these RNA-seq data, we have identified 8 genes that most likely participate in secondary cell wall synthesis, which include 3 cellulose synthase genes and 5 cellulose synthase-like genes. In parallel, a gene co-expression network was also constructed based on transcriptome sequencing.ConclusionsIn this study, we have identified a total of 46 cell wall synthesis genes in B. pendula, which include 8 secondary cell wall synthesis genes. These analyses will help decipher the genetic information of the cell wall synthesis genes, elucidate the molecular mechanism of cellulose synthesis and understand the cell wall structure in B. pendula.

scholarly journals Plant Defense Genes Associated with Quantitative Resistance to Potato Late Blight in Solanum phureja × Dihaploid S. tuberosum Hybrids

2002 ◽  
Vol 15 (6) ◽  
pp. 587-597 ◽  
Author(s):  
Friederike Trognitz ◽  
Patricia Manosalva ◽  
Rene Gysin ◽  
David Niño-Liu ◽  
Reinhard Simon ◽  
...  
Keyword(s):  
Late Blight ◽  
Plant Defense ◽  
Permutation Test ◽  
Quantitative Resistance ◽  
Late Blight Resistance ◽  
Gene Families ◽  
Phenylpropanoid Pathway ◽  
Defense Genes ◽  
Plant Defense Genes ◽  
Hybridization Probes

Markers corresponding to 27 plant defense genes were tested for linkage disequilibrium with quantitative resistance to late blight in a diploid potato population that had been used for mapping quantitative trait loci (QTLs) for late blight resistance. Markers were detected by using (i) hybridization probes for plant defense genes, (ii) primer pairs amplifying conserved domains of resistance (R) genes, (iii) primers for defense genes and genes encoding transcriptional regulatory factors, and (iv) primers allowing amplification of sequences flanking plant defense genes by the ligation-mediated polymerase chain reaction. Markers were initially screened by using the most resistant and susceptible individuals of the population, and those markers showing different allele frequencies between the two groups were mapped. Among the 308 segregating bands detected, 24 loci (8%) corresponding to six defense gene families were associated with resistance at χ2 ≥ 13, the threshold established using the permutation test at P = 0.05. Loci corresponding to genes related to the phenylpropanoid pathway (phenylalanine ammonium lyase [PAL], chalcone isomerase [CHI], and chalcone synthase [CHS]), loci related to WRKY regulatory genes, and other defense genes (osmotin and a Phytophthora infestans-induced cytochrome P450) were significantly associated with quantitative disease resistance. A subset of markers was tested on the mapping population of 94 individuals. Ten defense-related markers were clustered at a QTL on chromosome III, and three defense-related markers were located at a broad QTL on chromosome XII. The association of candidate genes with QTLs is a step toward understanding the molecular basis of quantitative resistance to an important plant disease.

scholarly journals Distinctive expansion of gene families associated with plant cell wall degradation, secondary metabolism, and nutrient uptake in the genomes of grapevine trunk pathogens

BMC Genomics ◽  
2015 ◽  
Vol 16 (1) ◽  
Author(s):  
Abraham Morales-Cruz ◽  
Katherine C. H. Amrine ◽  
Barbara Blanco-Ulate ◽  
Daniel P. Lawrence ◽  
Renaud Travadon ◽  
...  
Keyword(s):  
Cell Wall ◽  
Nutrient Uptake ◽  
Secondary Metabolism ◽  
Plant Cell ◽  
Plant Cell Wall ◽  
Gene Families ◽  
Cell Wall Degradation ◽  
Plant Cell Wall Degradation

scholarly journals Dual RNA Sequencing of Vitis vinifera during Lasiodiplodia theobromae Infection Unveils Host–Pathogen Interactions

2019 ◽  
Vol 20 (23) ◽  
pp. 6083 ◽  
Author(s):  
Micael Gonçalves ◽  
Rui Nunes ◽  
Laurentijn Tilleman ◽  
Yves Van de Peer ◽  
Dieter Deforce ◽  
...  
Keyword(s):  
Cell Wall ◽  
Vitis Vinifera ◽  
Rna Sequencing ◽  
Defense Mechanisms ◽  
Molecular Mechanisms ◽  
Plant Cell Wall ◽  
Phenylpropanoid Pathway ◽  
Cell Wall Modification ◽  
Lasiodiplodia Theobromae ◽  
Antimicrobial Substances

Lasiodiplodia theobromae is one of the most aggressive agents of the grapevine trunk disease Botryosphaeria dieback. Through a dual RNA-sequencing approach, this study aimed to give a broader perspective on the infection strategy deployed by L. theobromae, while understanding grapevine response. Approximately 0.05% and 90% of the reads were mapped to the genomes of L. theobromae and Vitis vinifera, respectively. Over 2500 genes were significantly differentially expressed in infected plants after 10 dpi, many of which are involved in the inducible defense mechanisms of grapevines. Gene expression analysis showed changes in the fungal metabolism of phenolic compounds, carbohydrate metabolism, transmembrane transport, and toxin synthesis. These functions are related to the pathogenicity mechanisms involved in plant cell wall degradation and fungal defense against antimicrobial substances produced by the host. Genes encoding for the degradation of plant phenylpropanoid precursors were up-regulated, suggesting that the fungus could evade the host defense response using the phenylpropanoid pathway. The up-regulation of many distinct components of the phenylpropanoid pathway in plants supports this hypothesis. Moreover, genes related to phytoalexin biosynthesis, hormone metabolism, cell wall modification enzymes, and pathogenesis-related proteins seem to be involved in the host responses observed. This study provides additional insights into the molecular mechanisms of L. theobromae and V. vinifera interactions.

scholarly journals Microbial Endoxylanases: Effective Weapons to Breach the Plant Cell-Wall Barrier or, Rather, Triggers of Plant Defense Systems?

2006 ◽  
Vol 19 (10) ◽  
pp. 1072-1081 ◽  
Author(s):  
Tim Beliën ◽  
Steven Van Campenhout ◽  
Johan Robben ◽  
Guido Volckaert
Keyword(s):  
Cell Wall ◽  
Plant Defense ◽  
Plant Cell ◽  
Cell Walls ◽  
Defense Mechanisms ◽  
Plant Cell Wall ◽  
Cell Wall Degrading Enzymes ◽  
Defense Systems ◽  
Xylanase Inhibitor

Endo-β-1,4-xylanases (EC 3.2.1.8) are key enzymes in the degradation of xylan, the predominant hemicellulose in the cell walls of plants and the second most abundant polysaccharide on earth. A number of endoxylanases are produced by microbial phytopathogens responsible for severe crop losses. These enzymes are considered to play an important role in phytopathogenesis, as they provide essential means to the attacking organism to break through the plant cell wall. Plants have evolved numerous defense mechanisms to protect themselves against invading pathogens, amongst which are proteinaceous inhibitors of cell wall-degrading enzymes. These defense mechanisms are triggered when a pathogen-derived elicitor is recognized by the plant. In this review, the diverse aspects of endoxylanases in promoting virulence and in eliciting plant defense systems are highlighted. Furthermore, the role of the relatively recently discovered cereal endoxylanase inhibitor families TAXI (Triticum aestivum xylanase inhibitor) and XIP (xylanase inhibitor protein) in plant defense is discussed.

scholarly journals A Genomic Perspective on the Evolutionary Diversity of the Plant Cell Wall

Plants ◽  
2020 ◽  
Vol 9 (9) ◽  
pp. 1195 ◽  
Author(s):  
Ryusuke Yokoyama
Keyword(s):  
Cell Wall ◽  
Plant Species ◽  
Functional Diversity ◽  
Plant Cell ◽  
Cell Walls ◽  
Plant Cell Wall ◽  
Dynamic Structure ◽  
Gene Families ◽  
Plant Genome ◽  
History Of

The plant cell wall is a complex and dynamic structure composed of numerous different molecules that play multiple roles in all aspects of plant life. Currently, a new frontier in biotechnology is opening up, which is providing new insights into the structural and functional diversity of cell walls, and is thus serving to re-emphasize the significance of cell wall divergence in the evolutionary history of plant species. The ever-increasing availability of plant genome datasets will thus provide an invaluable basis for enhancing our knowledge regarding the diversity of cell walls among different plant species. In this review, as an example of a comparative genomics approach, I examine the diverse patterns of cell wall gene families among 100 species of green plants, and illustrate the evident benefits of using genome databases for studying cell wall divergence. Given that the growth and development of all types of plant cells are intimately associated with cell wall dynamics, gaining a further understanding of the functional diversity of cell walls in relation to diverse biological events will make significant contributions to a broad range of plant sciences.

scholarly journals The Expression of a Bean PGIP in Transgenic Wheat Confers Increased Resistance to the Fungal Pathogen Bipolaris sorokiniana

2008 ◽  
Vol 21 (2) ◽  
pp. 171-177 ◽  
Author(s):  
Michela Janni ◽  
Luca Sella ◽  
Francesco Favaron ◽  
Ann E. Blechl ◽  
Giulia De Lorenzo ◽  
...  
Keyword(s):  
Cell Wall ◽  
Plant Defense ◽  
Fungal Pathogen ◽  
Plant Pathogens ◽  
Plant Cell Wall ◽  
Wide Spectrum ◽  
Control Plant ◽  
Bipolaris Sorokiniana ◽  
Transgenic Wheat ◽  
Cell Wall Polysaccharides

A possible strategy to control plant pathogens is the improvement of natural plant defense mechanisms against the tools that pathogens commonly use to penetrate and colonize the host tissue. One of these mechanisms is represented by the host plant's ability to inhibit the pathogen's capacity to degrade plant cell wall polysaccharides. Polygalacturonase-inhibiting proteins (PGIP) are plant defense cell wall glycoproteins that inhibit the activity of fungal endopolygalacturonases (endo-PGs). To assess the effectiveness of these proteins in protecting wheat from fungal pathogens, we produced a number of transgenic wheat lines expressing a bean PGIP (PvPGIP2) having a wide spectrum of specificities against fungal PGs. Three independent transgenic lines were characterized in detail, including determination of the levels of PvPGIP2 accumulation and its subcellular localization and inhibitory activity. Results show that the transgene-encoded protein is correctly secreted into the apoplast, maintains its characteristic recognition specificities, and endows the transgenic wheat with new PG recognition capabilities. As a consequence, transgenic wheat tissue showed increased resistance to digestion by the PG of Fusarium moniliforme. These new properties also were confirmed at the plant level during interactions with the fungal pathogen Bipolaris sorokiniana. All three lines showed significant reductions in symptom progression (46 to 50%) through the leaves following infection with this pathogen. Our results illustrate the feasibility of improving wheat's defenses against pathogens by expression of proteins with new capabilities to counteract those produced by the pathogens.

scholarly journals Identification of Putative Cell Wall Synthesis Genes in Betula pendula

2020 ◽  
Author(s):  
Song Chen ◽  
Xiyang Zhao ◽  
Su Chen
Keyword(s):  
Cell Wall ◽  
Transcriptome Sequencing ◽  
Betula Pendula ◽  
Plant Cell Wall ◽  
Gene Families ◽  
Transcript Abundance ◽  
Rna Seq ◽  
Cell Wall Synthesis ◽  
Biochemical Processes ◽  
External Cues

Abstract Cellulose is an essential structural component of the plant cell wall and is an important resource for the production of paper, textiles, bioplastics and other biomaterials. The synthesis of cellulose is among the most important but poorly understood biochemical processes, which is precisely regulated by internal and external cues. Here we identified 46 gene models in 7 gene families which encoding cellulose synthase and related enzymes of Betula pendula , and the transcript abundance of these genes in xylem, root, leaf and flower tissues also be determined. Based on these RNA-seq data, we have identified 8 genes that most likely participate in cell wall synthesis. In parallel, a gene co-expression network was also constructed based on transcriptome sequencing.

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