scholarly journals Molecular Characterization of Echinococcus granulosus sensu lato from Humans in Slovenia

Pathogens ◽  
2020 ◽  
Vol 9 (7) ◽  
pp. 562
Author(s):  
Barbara Šoba ◽  
Špela Gašperšič ◽  
Darja Keše ◽  
Tadeja Kotar
Keyword(s):  
Molecular Characterization ◽  
Echinococcus Granulosus ◽  
Nadh Dehydrogenase ◽  
Sensu Stricto ◽  
Intermediate Hosts ◽  
Western Balkans ◽  
Nadh Dehydrogenase Subunit ◽  
Important Intermediate ◽  
Echinococcus Granulosus Sensu Lato

The larval form of tapeworms of the Echinococcus granulosus sensu lato species cluster cause an important zoonotic infection, cystic echinococcosis (CE). Molecular characterization of the cluster’s isolates from different hosts greatly contributes to a better understanding of its transmission dynamics. To date, no genetic information is available on CE in Slovenia. In this work, we characterized isolates from human CE cases. Parasite samples from 18 patients were collected, together with the patients’ demographic and clinical data. Genomic DNA was analyzed by conventional PCR and sequencing at four mitochondrial loci (cytochrome c oxidase subunit 1, cox1; NADH dehydrogenase subunit 1, nad1; NADH dehydrogenase subunit 5, nad5; and small ribosomal RNA, rrnS). Thirteen isolates were successfully amplified and sequenced. Seven (58.8%) patients were infected with E. granulosus sensu stricto (s.s.) G1, five (38.5%) with E. canadensis G7 and one (7.7%) with E. granulosus s.s. G3. Echinococcus canadensis G7, the pig genotype, was identified exclusively in autochthonous Slovenes, while the patients originating from the Western Balkans were all infected with E. granulosus s.s. Our findings suggest that pigs are important intermediate hosts for human CE in Slovenia.

Prevalence and genotyping of Echinococcus granulosus sensu lato from livestock in north-eastern Kenya

2020 ◽  
Vol 94 ◽  
Author(s):  
H.A. Omondi ◽  
G. Gitau ◽  
P. Gathura ◽  
E. Mulinge ◽  
E. Zeyhle ◽  
...  
Keyword(s):  
Echinococcus Granulosus ◽  
Nadh Dehydrogenase ◽  
Sensu Stricto ◽  
The North ◽  
North Eastern ◽  
Polymerase Chain ◽  
First Time ◽  
Isiolo County ◽  
Echinococcus Granulosus Sensu Lato

Abstract Cystic echinococcosis (CE) is a zoonotic disease of cosmopolitan distribution and caused by the larval stage of the dog tapeworm, Echinococcus granulosus sensu lato (s.l.). CE occurs in the wider African continent and in Kenya, notably in the Maasailand and Turkana regions; however, recent studies demonstrate its presence in other parts of Kenya. This study determined the occurrence of CE in livestock (camels, goats, sheep and cattle) in Isiolo, Garissa and Wajir counties, and characterized the species of E. granulosus s.l. present. An abattoir survey was used to determine the presence of CE in various organs in livestock. Polymerase chain reaction-restriction fragment length polymorphism and sequencing of the mitochondrial NADH dehydrogenase subunit 1 gene was used for genotyping. A total of 1368 carcasses from 687 goats, 234 camels, 329 sheep and 118 cattle were inspected for the presence of hydatid cysts. The overall proportion of infections was 29.1% in camels, 14.4% in cattle, 9.9% in goats and 8.2% in sheep. The liver was the most infected organ, while only the lung of camels harboured fertile cysts. Of the 139 cysts genotyped, 111 (79.9%) belonged to Echinococcus canadensis (G6/7) and 20 (14.4%) to E. granulosus sensu stricto. One and two cysts were identified as Taenia saginata and unknown Taenia species, respectively. There was a significant association between county of origin and species of the animal with occurrence of CE. This study reports, for the first time, the characterization of Echinococcus species in livestock from Garissa and Wajir counties, and the current situation in Isiolo county. The fertility of cysts in camels and frequency of E. canadensis (G6/7) in all livestock species indicate that camels play an important role in the maintenance of CE in the north-eastern counties of Kenya.

Molecular characterization of the horse isolate of Echinococcus granulosus in Turkey

2012 ◽  
Vol 87 (3) ◽  
pp. 305-308 ◽  
Author(s):  
A.E. Utuk ◽  
S. Simsek
Keyword(s):  
Molecular Characterization ◽  
Echinococcus Granulosus ◽  
Pcr Amplification ◽  
Sensu Stricto ◽  
12S Rrna ◽  
Intermediate Hosts ◽  
Chain Reaction ◽  
Mitochondrial 12S Rrna ◽  
Polymerase Chain

AbstractCystic echinococcosis is one of the most important helminthozoonoses, affecting various species of intermediate hosts and humans. In this report, we present Echinococcus granulosus infection in a horse and its molecular characterization. Polymerase chain reaction (PCR) amplification of mitochondrial 12S rRNA (mt-12S rRNA) and partial sequencing of mitochondrial cytochrome c oxidase subunit 1 (mt-CO1) genes were performed. According to the mt-12S rRNA-PCR result, the horse isolate was grouped with E. granulosus sensu stricto (G1–G3) and the partial mt-CO1 sequence corresponded to the G1 strain. This is the first study of the molecular characterization of the horse isolate of E. granulosus in Turkey.

scholarly journals Cystic Echinococcosis: Clinical, Immunological, and Biomolecular Evaluation of Patients from Sardinia (Italy)

Pathogens ◽  
2020 ◽  
Vol 9 (11) ◽  
pp. 907
Author(s):  
Cinzia Santucciu ◽  
Piero Bonelli ◽  
Angela Peruzzu ◽  
Alessandro Fancellu ◽  
Vincenzo Marras ◽  
...  
Keyword(s):  
Molecular Biology ◽  
Echinococcus Granulosus ◽  
Cystic Echinococcosis ◽  
Public Health Problem ◽  
Sensu Stricto ◽  
Surgical Removal ◽  
Neighbor Joining ◽  
Intermediate Hosts ◽  
High Prevalence ◽  
Echinococcus Granulosus Sensu Lato

Cystic echinococcosis (CE), a zoonotic disease caused by the larval stage of the tapeworm Echinococcus granulosus sensu lato (s.l.), is a worldwide public health problem. Echinococcus granulosus sensu stricto (s.s.), associated with G1 and G3 genotypes, is endemic with high prevalence in the Mediterranean basin. The parasite’s life cycle comprises definitive hosts (canids) and intermediate hosts (ruminants) and can occasionally involve humans. The main aim of this research was to confirm the diagnosis of 13 patients suspected of CE who presented different complications and needed the surgical removal of the cysts. We also wanted to understand and clarify more the diagnosis of echinococcosis in humans. For this purpose, the patients first underwent cyst evaluation by ultrasound (US), immunological analysis, and then total pericystectomy, followed by parasitological, histopathological, and molecular biology examinations of the cysts. US stadiated one CE1, one CE2, eight CE3b, one CE4, and two CE5; immunology evidenced nine positives; histopathology confirmed 11 CE cysts, of which 8 fertile presenting protoscoleces were identified as E. granulosus s.s. by molecular biology, genotyped as three G1 and four G3 by neighbor-joining (NJ) phylogenetic tree. In conclusion, the results showed that 11 patients were affected by E. granulosus s.s. G1 orG3, and 2 cystic neoformations were of non-parasitic origin.

Use of FTA® card methodology for sampling and molecular characterization of Echinococcus granulosus sensu lato in Africa

2017 ◽  
Vol 173 ◽  
pp. 29-33 ◽  
Author(s):  
Franck Boué ◽  
Ikhlass El Berbri ◽  
Vanessa Hormaz ◽  
Jean-Marc Boucher ◽  
Ahmed Bezeid El Mamy ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Echinococcus Granulosus ◽  
Echinococcus Granulosus Sensu Lato

scholarly journals Molecular Characterization of Echinococcus granulosus Sensu Lato from Farm Animals in Egypt

PLoS ONE ◽  
2015 ◽  
Vol 10 (3) ◽  
pp. e0118509 ◽  
Author(s):  
Said Amer ◽  
Ibrahim B. Helal ◽  
Evelyne Kamau ◽  
Yaoyu Feng ◽  
Lihua Xiao
Keyword(s):  
Molecular Characterization ◽  
Echinococcus Granulosus ◽  
Farm Animals ◽  
Echinococcus Granulosus Sensu Lato

scholarly journals Prevalence and Molecular Characterization of Echinococcus granulosus Sensu Stricto in Northern Xinjiang, China

2019 ◽  
Vol 57 (2) ◽  
pp. 153-159 ◽  
Author(s):  
Baoping Guo ◽  
Zhuangzhi Zhang ◽  
Xueting Zheng ◽  
Yongzhong Guo ◽  
Gang Guo ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Echinococcus Granulosus ◽  
Sensu Stricto ◽  
Northern Xinjiang ◽  
Echinococcus Granulosus Sensu Stricto

Molecular characterization of Echinococcus granulosus sensu stricto and Echinococcus canadensis in humans and livestock from Algeria

2016 ◽  
Vol 115 (6) ◽  
pp. 2423-2431 ◽  
Author(s):  
Houria Zait ◽  
Mokhtaria Kouidri ◽  
Florence Elisabeth Grenouillet ◽  
Gérald Umhang ◽  
Laurence Millon ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Echinococcus Granulosus ◽  
Sensu Stricto ◽  
Echinococcus Canadensis ◽  
Echinococcus Granulosus Sensu Stricto

scholarly journals Molecular characterization of Echinococcus granulosus sensu stricto isolated from livestock of Qazvin, Iran

2020 ◽  
Vol 20 ◽  
Author(s):  
Mojtaba Shahnazi ◽  
Maryam Habibvand ◽  
Morteza Ghanbari Johkool ◽  
Elham Hajialilo ◽  
Meysam Sharifdini ◽  
...  
Keyword(s):  
Echinococcus Granulosus ◽  
Haplotype Diversity ◽  
Sensu Stricto ◽  
Cox1 Gene ◽  
Intermediate Hosts ◽  
Study Region ◽  
Predominant Genotype ◽  
Mitochondrial Cox1 ◽  
Significant Difference

Introduction: Hydatidiosis is a serious parasitic disease in humans and livestock, worldwide. Echinococcus granulosus shows notable genetic variation among intermediate hosts. Several genotypes of the worm have been reported from different parts of Iran, but no information over the parasite genotypes status in the study region is available. The current study investigated the presence of different genotypes of E. granulosus in the livestock of Qazvin, Iran, by sequencing the mitochondrial Cox1 genes. Methodology: One hundred twenty E. granulosus isolates, including 30 from goats, 40 from cattle and 50 from sheep were collected from the slaughterhouses in Qazvin province. Mitochondrial Cox1 gene region was amplified by PCR and 30 isolates were sequenced. Phylogenetic analysis was done by using the MEGA 7.0 software. Morphological analysis was performed on rostellar hook length of protoscoleces. Result: All isolates were identified as E. granulosus sensu stricto (G1-G3 complex) among 17% of isolates clarified as G3 genotypes. G1 was the predominant genotype among the specimens. No significant difference between the rostellar hooks measurements of different genotypes was observed. Conclusion: Our findings confirmed the presence of E. granulosus sensu stricto in the region although further studies are required to determine the haplotype diversity of E. granulosus using different mitochondrial and nuclear genes.

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