Molecular characterization of Echinococcus granulosus sensu stricto isolated from livestock of Qazvin, Iran

Introduction: Hydatidiosis is a serious parasitic disease in humans and livestock, worldwide. Echinococcus granulosus shows notable genetic variation among intermediate hosts. Several genotypes of the worm have been reported from different parts of Iran, but no information over the parasite genotypes status in the study region is available. The current study investigated the presence of different genotypes of E. granulosus in the livestock of Qazvin, Iran, by sequencing the mitochondrial Cox1 genes. Methodology: One hundred twenty E. granulosus isolates, including 30 from goats, 40 from cattle and 50 from sheep were collected from the slaughterhouses in Qazvin province. Mitochondrial Cox1 gene region was amplified by PCR and 30 isolates were sequenced. Phylogenetic analysis was done by using the MEGA 7.0 software. Morphological analysis was performed on rostellar hook length of protoscoleces. Result: All isolates were identified as E. granulosus sensu stricto (G1-G3 complex) among 17% of isolates clarified as G3 genotypes. G1 was the predominant genotype among the specimens. No significant difference between the rostellar hooks measurements of different genotypes was observed. Conclusion: Our findings confirmed the presence of E. granulosus sensu stricto in the region although further studies are required to determine the haplotype diversity of E. granulosus using different mitochondrial and nuclear genes.

Download Full-text

Molecular Characterization of Echinococcus granulosus sensu lato from Humans in Slovenia

Pathogens ◽

10.3390/pathogens9070562 ◽

2020 ◽

Vol 9 (7) ◽

pp. 562

Author(s):

Barbara Šoba ◽

Špela Gašperšič ◽

Darja Keše ◽

Tadeja Kotar

Keyword(s):

Molecular Characterization ◽

Echinococcus Granulosus ◽

Nadh Dehydrogenase ◽

Sensu Stricto ◽

Intermediate Hosts ◽

Western Balkans ◽

Nadh Dehydrogenase Subunit ◽

Important Intermediate ◽

Echinococcus Granulosus Sensu Lato

The larval form of tapeworms of the Echinococcus granulosus sensu lato species cluster cause an important zoonotic infection, cystic echinococcosis (CE). Molecular characterization of the cluster’s isolates from different hosts greatly contributes to a better understanding of its transmission dynamics. To date, no genetic information is available on CE in Slovenia. In this work, we characterized isolates from human CE cases. Parasite samples from 18 patients were collected, together with the patients’ demographic and clinical data. Genomic DNA was analyzed by conventional PCR and sequencing at four mitochondrial loci (cytochrome c oxidase subunit 1, cox1; NADH dehydrogenase subunit 1, nad1; NADH dehydrogenase subunit 5, nad5; and small ribosomal RNA, rrnS). Thirteen isolates were successfully amplified and sequenced. Seven (58.8%) patients were infected with E. granulosus sensu stricto (s.s.) G1, five (38.5%) with E. canadensis G7 and one (7.7%) with E. granulosus s.s. G3. Echinococcus canadensis G7, the pig genotype, was identified exclusively in autochthonous Slovenes, while the patients originating from the Western Balkans were all infected with E. granulosus s.s. Our findings suggest that pigs are important intermediate hosts for human CE in Slovenia.

Download Full-text

Phylogeography and Genetic Diversity of Human Hydatidosis in Bordering the Caspian Sea, Northern Iran by Focusing on Echinococcus granulosus Sensu Stricto Complex

Iranian Journal of Public Health ◽

10.18502/ijph.v49i9.4097 ◽

2020 ◽

Author(s):

Abolghasem SIYADATPANAH ◽

Ahmad DARYANI ◽

Shahabeddin SARVI ◽

Adel SPOTIN ◽

Mehdi SHARIF ◽

...

Keyword(s):

Genetic Diversity ◽

Echinococcus Granulosus ◽

Haplotype Diversity ◽

Taxonomic Status ◽

Sensu Stricto ◽

Ffpe Tissue ◽

Cox1 Gene ◽

Northern Iran ◽

Tissue Samples ◽

Echinococcus Granulosus Sensu Stricto

Background: Human Echinococcosisis a cyclo-zoonotic infection caused by tapeworms of the Echinococcus granulosus sensu stricto complex. The detection of mitochondrial genome data of genus Echinococcus can reflect the taxonomic status, genetic diversity, and population structure genetics. Methods: Totally, 52 formalin-fixed paraffin-embedded (FFPE) tissue samples from patients with histologically confirmed CE were collected from Mazandaran province, Iran in the period of Mar 1995 to May 2018. All extracted DNAs from (FFPE) tissue samples were subjected to amplify by polymerase chain reactions method targeting cytochrome c oxidase subunit 1 (cox1) gene. All PCR amplicons were sequenced to phylogenetic analysis and genetic diversity. Results: Molecular analysis showed that 50(96.1%) and 2 (3.84%) isolates were identified as G1 andG3 E. granulosus genotypes, respectively. DNA sequence analyses indicated a high gene diversity for G1 (Haplotype diversity: 0.830) and G3 genotypes (Hd: 1.00). Based on multiple sequence alignment analyses, 7 (13.46%; G1 genotype) and 2 (3.84%; G3 genotype) new haplotypes were unequivocally identified. Conclusion: G3 genotype (Buffalo strain) was identified from two human hydatidosis isolates in the region. Present study strengthens our knowledge about taxonomic status, transmission patterns of Echinococcus parasite to human and heterogeneity aspects of this parasite in clinical CE isolates of Northern Iran.

Download Full-text

Molecular characterization of the horse isolate of Echinococcus granulosus in Turkey

Journal of Helminthology ◽

10.1017/s0022149x12000363 ◽

2012 ◽

Vol 87 (3) ◽

pp. 305-308 ◽

Cited By ~ 10

Author(s):

A.E. Utuk ◽

S. Simsek

Keyword(s):

Molecular Characterization ◽

Echinococcus Granulosus ◽

Pcr Amplification ◽

Sensu Stricto ◽

12S Rrna ◽

Intermediate Hosts ◽

Chain Reaction ◽

Mitochondrial 12S Rrna ◽

Polymerase Chain

AbstractCystic echinococcosis is one of the most important helminthozoonoses, affecting various species of intermediate hosts and humans. In this report, we present Echinococcus granulosus infection in a horse and its molecular characterization. Polymerase chain reaction (PCR) amplification of mitochondrial 12S rRNA (mt-12S rRNA) and partial sequencing of mitochondrial cytochrome c oxidase subunit 1 (mt-CO1) genes were performed. According to the mt-12S rRNA-PCR result, the horse isolate was grouped with E. granulosus sensu stricto (G1–G3) and the partial mt-CO1 sequence corresponded to the G1 strain. This is the first study of the molecular characterization of the horse isolate of E. granulosus in Turkey.

Download Full-text

The optimum cut-off value to differentiate Echinococcus granulosus sensu stricto from other species of E. granulosus sensu lato using larval rostellar hook morphometry

Journal of Helminthology ◽

10.1017/s0022149x13000473 ◽

2013 ◽

Vol 89 (1) ◽

pp. 1-8 ◽

Cited By ~ 2

Author(s):

S.V. Soriano ◽

N.B. Pierangeli ◽

L.A. Pianciola ◽

M. Mazzeo ◽

L.E. Lazzarini ◽

...

Keyword(s):

Molecular Epidemiology ◽

Echinococcus Granulosus ◽

Cystic Echinococcosis ◽

Control Strategies ◽

High Sensitivity ◽

Field Studies ◽

Sensu Stricto ◽

Roc Curve Analysis ◽

First Time

AbstractCystic echinococcosis caused by Echinococcus granulosus sensu lato is one of the most important helminth zoonoses in the world; it affects both humans and livestock. The disease is endemic in Argentina and highly endemic in the province of Neuquén. Considerable genetic and phenotypic variation has been demonstrated in E. granulosus, and ten different genotypes (G1–G10) have been identified using molecular tools. Echinococcus granulosus sensu lato may be considered a species complex, comprised of E. granulosus sensu stricto (G1–G3), E. equinus (G4), E. ortleppi (G5) and E. canadensis (G6–G10). In endemic areas, the characterization of cystic echinococcosis molecular epidemiology is important in order to apply adequate control strategies. A cut-off value for larval large hook total length to distinguish E. granulosus sensu stricto isolates from those produced by other species of the complex was defined for the first time. Overall, 1780 larval hooks of 36 isolates obtained from sheep (n= 11, G1), goats (n= 10, G6), cattle (n= 5, G6) and pigs (n= 10, G7) were analysed. Validation against molecular genotyping as gold standard was carried out using the receiver operating characteristic (ROC) curve analysis. The optimum cut-off value was defined as 26.5 μm. The proposed method showed high sensitivity (97.8%) and specificity (91.1%). Since in most endemic regions the molecular epidemiology of echinococcosis includes the coexistence of the widely distributed E. granulosus sensu stricto G1 strain and other species of the complex, this technique could be useful as a quick and economical tool for epidemiological and surveillance field studies, when fertile cysts are present.

Download Full-text

Cystic Echinococcosis: Clinical, Immunological, and Biomolecular Evaluation of Patients from Sardinia (Italy)

Pathogens ◽

10.3390/pathogens9110907 ◽

2020 ◽

Vol 9 (11) ◽

pp. 907

Author(s):

Cinzia Santucciu ◽

Piero Bonelli ◽

Angela Peruzzu ◽

Alessandro Fancellu ◽

Vincenzo Marras ◽

...

Keyword(s):

Molecular Biology ◽

Echinococcus Granulosus ◽

Cystic Echinococcosis ◽

Public Health Problem ◽

Sensu Stricto ◽

Surgical Removal ◽

Neighbor Joining ◽

Intermediate Hosts ◽

High Prevalence ◽

Echinococcus Granulosus Sensu Lato

Cystic echinococcosis (CE), a zoonotic disease caused by the larval stage of the tapeworm Echinococcus granulosus sensu lato (s.l.), is a worldwide public health problem. Echinococcus granulosus sensu stricto (s.s.), associated with G1 and G3 genotypes, is endemic with high prevalence in the Mediterranean basin. The parasite’s life cycle comprises definitive hosts (canids) and intermediate hosts (ruminants) and can occasionally involve humans. The main aim of this research was to confirm the diagnosis of 13 patients suspected of CE who presented different complications and needed the surgical removal of the cysts. We also wanted to understand and clarify more the diagnosis of echinococcosis in humans. For this purpose, the patients first underwent cyst evaluation by ultrasound (US), immunological analysis, and then total pericystectomy, followed by parasitological, histopathological, and molecular biology examinations of the cysts. US stadiated one CE1, one CE2, eight CE3b, one CE4, and two CE5; immunology evidenced nine positives; histopathology confirmed 11 CE cysts, of which 8 fertile presenting protoscoleces were identified as E. granulosus s.s. by molecular biology, genotyped as three G1 and four G3 by neighbor-joining (NJ) phylogenetic tree. In conclusion, the results showed that 11 patients were affected by E. granulosus s.s. G1 orG3, and 2 cystic neoformations were of non-parasitic origin.

Download Full-text

Genetic characterization of human hydatid cysts shows coinfection by Echinococcus canadensis G7 and Echinococcus granulosus sensu stricto G1 in Argentina

Parasitology Research ◽

10.1007/s00436-017-5555-2 ◽

2017 ◽

Vol 116 (9) ◽

pp. 2599-2604 ◽

Cited By ~ 4

Author(s):

María Florencia Debiaggi ◽

Silvia Viviana Soriano ◽

Nora Beatriz Pierangeli ◽

Lorena Evelina Lazzarini ◽

Luis Alfredo Pianciola ◽

...

Keyword(s):

Echinococcus Granulosus ◽

Genetic Characterization ◽

Sensu Stricto ◽

Hydatid Cysts ◽

Echinococcus Canadensis ◽

Echinococcus Granulosus Sensu Stricto

Download Full-text

Genetic characterization of livestock and human hydatid cyst isolates from northwest Iran, using the mitochondrial cox1 gene sequence

Parasitology Research ◽

10.1007/s00436-015-4673-y ◽

2015 ◽

Vol 114 (12) ◽

pp. 4363-4370 ◽

Cited By ~ 20

Author(s):

Mehdi Farhadi ◽

Asghar Fazaeli ◽

Ali Haniloo

Keyword(s):

Hydatid Cyst ◽

Gene Sequence ◽

Genetic Characterization ◽

Cox1 Gene ◽

Mitochondrial Cox1 ◽

Northwest Iran

Download Full-text

The prevalence, intensity and viability of hydatid cysts in slaughtered animals in the Ardabil province of Northwest Iran

Journal of Helminthology ◽

10.1017/s0022149x0720731x ◽

2007 ◽

Vol 81 (1) ◽

pp. 13-17 ◽

Cited By ~ 67

Author(s):

A. Daryani ◽

R. Alaei ◽

R. Arab ◽

M. Sharif ◽

M.H. Dehghan ◽

...

Keyword(s):

Echinococcus Granulosus ◽

Cross Sectional Study ◽

Sectional Study ◽

Hydatid Cysts ◽

Intermediate Hosts ◽

Cross Sectional ◽

Significant Difference ◽

Important Intermediate ◽

Northwest Iran

AbstractIn a cross-sectional study, a total of 5381 slaughtered animals, namely 928 cattle, 243 buffaloes, 3765 sheep and 445 goats were inspected macroscopically for hydatid cysts in northwest Iran, with prevalence values of 38.3%, 11.9%, 74.4% and 20%, respectively, being recorded. Prevalences were higher in females compared with males, but a significant difference (P < 0.001) was only found in sheep and cattle. Most cases which were condemned were seen in the lungs of sheep (13.4%) indicating that sheep are the most important intermediate hosts for Echinococcus granulosus in this area.

Download Full-text