Optimization and Transfollicular Delivery of Finasteride-Loaded Proniosomes for Hair Growth Stimulation in C57BL/6Mlac Mice

The study aimed to develop the finasteride-loaded proniosome (FLP) to enhance the transfollicular delivery of finasteride (FN). The response surface methodology (RSM) combined with central composite design (CCD) with three independent variables (FN concentrations, total lipid content, and cholesterol content) was used to optimize the FLP preparation. The particles size, zeta potential, entrapment efficiency, and drug loading capacity of the FLP were analyzed. The transfollicular delivery of the optimum formulation was investigated in vitro. In vivo hair growth stimulation study was performed on C57BL/6Mlac mice dorsal areas. The Draize primary skin irritation test for erythema and edema was performed in the New Zealand white rabbit skin. The optimum FLP consists of 5.0 mM of FN, 10.1 mM of total lipid content, and 50.0% of the cholesterol in the total lipid. The prepared proniosome delivered the FN significantly (p < 0.05), compared to the naked finasteride solution in a dose- and time-dependent manner. The FLP treatment significantly increases the number and size of hair follicles in a dose-dependent manner. The efficiency of 1% FLP was comparable to the 2% minoxidil solution. The FLP exhibited no skin irritation after 72 h. Therefore, the results demonstrated that the FLP could stimulate hair growth via a transfollicular delivery system.

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Impact of Seasonal Changes on Composition of Total Lipid Content in Muscle, Liver and Testes of Freshwater Male Fish Mystus Cavasius (Ham) From Bhadra Reservoir, Shivamogga, Karnataka, India

International Journal of Scientific Research ◽

10.15373/22778179/feb2014/181 ◽

2012 ◽

Vol 3 (2) ◽

pp. 540-541

Author(s):

Ashashree H M Ashashree H M ◽

◽

B.Venkateshwarlu B B.Venkateshwarlu B ◽

Sayeswara HA Sayeswara HA

Keyword(s):

Total Lipid ◽

Lipid Content ◽

Seasonal Changes ◽

Total Lipid Content ◽

Male Fish

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Aspergillus flavusgrowth and aflatoxin production as influenced by total lipid content during growth and development of cottonseed

Journal of Crop Improvement ◽

10.1080/15427528.2016.1263811 ◽

2017 ◽

Vol 31 (1) ◽

pp. 91-99 ◽

Cited By ~ 2

Author(s):

Kanniah Rajasekaran ◽

Greg Ford ◽

Kandan Sethumadhavan ◽

Carol Carter-Wientjes ◽

John Bland ◽

...

Keyword(s):

Total Lipid ◽

Lipid Content ◽

Growth And Development ◽

Total Lipid Content ◽

Aflatoxin Production

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Comparison of biomass production and total lipid content of freshwater green microalgae cultivated under various culture conditions

Bioprocess and Biosystems Engineering ◽

10.1007/s00449-013-0920-8 ◽

2013 ◽

Vol 37 (2) ◽

pp. 99-106 ◽

Cited By ~ 45

Author(s):

Geun Ho Gim ◽

Jung Kon Kim ◽

Hyeon Seok Kim ◽

Mathur Nadarajan Kathiravan ◽

Hetong Yang ◽

...

Keyword(s):

Biomass Production ◽

Total Lipid ◽

Lipid Content ◽

Total Lipid Content ◽

Culture Conditions ◽

Green Microalgae

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Adipophilin and Perilipin 3 Correlate with Total Lipid Content in Human Breast Milk

10.20944/preprints201810.0008.v1 ◽

2018 ◽

Author(s):

Tereza Pavlova ◽

Zdenek Spacil ◽

Veronika Vidova ◽

Filip Zlamal ◽

Eliska Cechova ◽

...

Keyword(s):

Mass Spectrometry ◽

Breast Milk ◽

Total Lipid ◽

Lipid Content ◽

Gravimetric Method ◽

Total Lipid Content ◽

Milk Lipid ◽

Human Breast Milk ◽

Human Breast

Objective: Lipids are secreted into milk as bilayer-coated structures: milk lipid globules (MLGs). Adipophilin (ADRP) and perilipin 3 (TIP47) are associated with MLGs in human breast milk; however, the role of these proteins in milk lipid secretion is not fully understood. The aim of the study was to investigate levels of ADRP, TIP47 and total lipid content in human breast milk, their mutual correlations and dynamics during lactation. Research Methods & Procedures: Milk samples from 22 healthy lactating women (Caucasian, Central European) were collected at five time points during lactation (1–3, 12–14, 29–30, 88–90 and 178–180 days postpartum). Mass spectrometry-based method was used for quantification of ADRP and TIP47 in the samples. The gravimetric method was used to determine milk total lipid content. Results: We observed distinctive trends in ADRP, TIP47 levels and lipid content in human breast milk during the first 6 months of lactation. We also found a significant association between lipid content and ADRP, lipid content and TIP47, and ADRP and TIP47 concentrations in breast milk at all sampling points. Moreover, we derived an equation for estimating the mean lipid content of milk based on ADRP concentration in human breast milk. Conclusions: A mass spectrometry-based method was developed for quantifying ADRP and TIP47 in human breast milk. Strong mutual correlations were found between ADRP, TIP47 and total lipid content in human breast milk.

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