scholarly journals In Vivo Rodent Cervicothoracic Vasculature Imaging Using Photoacoustic Computed Tomography

Photonics ◽  
2021 ◽  
Vol 8 (8) ◽  
pp. 312
Author(s):  
Junsoo Lee ◽  
Hoseong Cho ◽  
Sungjo Park ◽  
Jaeyul Lee ◽  
Daewoon Seong ◽  
...  

Mice and rats are rodent specimens commonly used in multidisciplinary research. Specifically, vasculature imaging of rodents has been widely performed in preclinical studies using various techniques, such as computed tomography, magnetic resonance imaging, and ultrasound imaging. Photoacoustic CT (PACT) is a noninvasive, nonionizing optical imaging technique derived from photoacoustic tomography and benefits from using intrinsic endogenous contrast agents to produce three-dimensional volumetric data from images. In this study, a commercial PACT device was employed to assess the cervicothoracic vasculature of mouse and rat specimens, which has rarely been examined using PACT, under two conditions with depilation and skin incision. Various blood vessels, including the common carotid artery, internal/external jugular veins, cranial vena cava, internal thoracic vein, and mammary, were identified in the acquired PACT images. The difference between the depilated and skin-incised specimens also revealed the presence of branches from certain blood vessels and specific anatomical features such as the manubrium of the sternum. This study presents detailed PACT images observing the cervicothoracic vasculature of rodent specimens and is expected to be used as a reference for various preclinical experiments on mice and rats.

Author(s):  
A H A Baazil ◽  
J G G Dobbe ◽  
E van Spronsen ◽  
F A Ebbens ◽  
F G Dikkers ◽  
...  

Abstract Objective This study aimed to compare the necessary scutum defect for transmeatal visualisation of middle-ear landmarks between an endoscopic and microscopic approach. Method Human cadaveric heads were used. In group 1, middle-ear landmarks were visualised by endoscope (group 1 endoscopic approach) and subsequently by microscope (group 1 microscopic approach following endoscopy). In group 2, landmarks were visualised solely microscopically (group 2 microscopic approach). The amount of resected bone was evaluated via computed tomography scans. Results In the group 1 endoscopic approach, a median of 6.84 mm3 bone was resected. No statistically significant difference (Mann–Whitney U test, p = 0.163, U = 49.000) was found between the group 1 microscopic approach following endoscopy (median 17.84 mm3) and the group 2 microscopic approach (median 20.08 mm3), so these were combined. The difference between the group 1 endoscopic approach and the group 1 microscopic approach following endoscopy plus group 2 microscopic approach (median 18.16 mm3) was statistically significant (Mann–Whitney U test, p < 0.001, U = 18.000). Conclusion This study showed that endoscopic transmeatal visualisation of middle-ear landmarks preserves more of the bony scutum than a microscopic transmeatal approach.


2017 ◽  
Vol 87 (5) ◽  
pp. 631-639
Author(s):  
Mensur Šehić ◽  
◽  
Ivana Kiš ◽  
Dražen Vnuk ◽  
Dženita Hadžijunuzović Alagić ◽  
...  

2017 ◽  
Vol 21 (04) ◽  
pp. 366-370 ◽  
Author(s):  
Mohammad El-Anwar ◽  
Atef Hamed ◽  
Ghada Abdulmonaem ◽  
Ismail Elnashar ◽  
Inas Elfiki

Introduction The inferior turbinate (IT) is the most susceptible turbinate to enlargement causing nasal obstruction. The common belief ascribes most of the enlargement of the IT to mucosal elements. Objective This study aimed to investigate the detailed computed tomography (CT) measurement of the IT in asymptomatic adult by determining the thickness of both the non-bony (mucosa) and bony parts and their relation to nasal air space in different related areas of the nose. Methods We included in the study paranasal CT scans of 108 individuals (216 IT) that had no paranasal pathology. We acquired axial images with multiplanar reformates to obtain delicate details in coronal and sagittal planes for all subjects. We took separate measurements of the thickness of the medial mucosa, bones, and lateral mucosa of the IT on the anterior and posterior portions of ITs. We also measured the air space of the nasal cavity between the septum and IT anteriorly and posteriorly. Results The difference in the air space between nasal septum, anterior and posterior ends of IT was extremely statistically significant (P < 0.0001). The thickness of the medial mucosa was extremely significantly more than the lateral mucosa width (P < 0.0001). There was no significant difference in length of IT at both sides (p = 0.5781). Conclusion The detailed CT measurement of the IT in normal adult is an easy and novel measurement. This study lays the foundation for CT measurement of IT for further work that can describe changes in IT measures after turbinate surgery.


Author(s):  
Heydar Sadeghi ◽  
Hesam Fazlali ◽  
Saba Sadeghi ◽  
Seyedmojtaba Seyedmojtaba Ojaghi

Background: In athletes with anterior cruciate ligament (ACL) deficiencies could assess functional capabilities with different instruments such as use of a camera in vivo situation. However, these methods have suffered from a large number of limitations such as inability to be repeatable and complexity in technique. Objective: The main purpose of this study was to compare gait pattern of the athletes with ACL injury and able-bodied subjects using an accelerometer. Method: A three-dimensional accelerometer was placed over the tibia tuberosity of 20 healthy and 20 individuals with ACL-deficiencies (ACLD). After walking on the treadmill, the principal components of the acceleration data were calculated using MATLAB software. Results: In this study, Principle Component analysis was used for statistical analysis. The results indicated that subjects with ACL deficiency have different gait pattern compared to the control group. The major differences between stride trajectories of the two groups were at the end of mid-swing and the beginning of terminal swing phases in vertical axis. ACL deficient subjects exhibited different gait patterns during mid and terminal stance phases in anterior- posterior axis compared with normal controls. Conclusions: The difference in gait between subjects with ACL deficiency and healthy subjects are depends on variation in the amount of knee flexion and tibia rotation that could be altered to motor recruitment.


2016 ◽  
Vol 2 (3) ◽  
pp. 53-55
Author(s):  
R Nagesh ◽  
Anil K Shukla ◽  
P Hazif Backer ◽  
VV Seetha Pramila

ABSTRACT Introduction Superior vena cava syndrome results from the blockage of blood flow through the superior vena cava. It can be a medical emergency if it causes laryngeal or cerebral edema. Hence, prompt diagnosis is of utmost importance. Case report A 65-year-old male patient came with complaints of progressive dyspnea and cough since 3 weeks. A contrast- enhanced multidetector computed tomography (MDCT) scan was done for the patient, which showed thrombus in the superior vena cava with a rich network of collaterals. The three-dimensional reconstruction was also done to demonstrate the collaterals. Conclusion The MDCT provides an excellent tool to diagnose and assess the cause of superior vena cava obstruction. How to cite this article Backer PH, VVP Seetha, Shukla AK, Nagesh R. Multidetector Computed Tomography Evaluation of Malignant Superior Vena Cava Syndrome. J Med Sci 2016;2(3):53-55.


2005 ◽  
Vol 46 (3) ◽  
pp. 222-226 ◽  
Author(s):  
R. Röttgen ◽  
F. Fischbach ◽  
M. Plotkin ◽  
H. Herzog ◽  
T. Freund ◽  
...  

Purpose: To improve the sensitivity of computed tomography (CT) colonography in the detection of polyps by comparing the 3D reconstruction tool “colon dissection” and endoluminal view (virtual colonoscopy) with axial 2D reconstructions. Material and Methods: Forty‐eight patients (22 M, 26 F, mean age 57±21) were studied after intra‐anal air insufflation in the supine and prone positions using a 16‐slice helical CT (16×0.625 mm, pitch 1.7; detector rotation time 0.5 s; 160 mAs und 120 kV) and conventional colonoscopy. Two radiologists blinded to the results of the conventional colonoscopy analyzed the 3D reconstruction in virtual‐endoscopy mode, in colon‐dissection mode, and axial 2D slices. Results: Conventional colonoscopy revealed a total of 35 polyps in 15 patients; 33 polyps were disclosed by CT methods. Sensitivity and specificity for detecting colon polyps were 94% and 94%, respectively, when using the “colon dissection”, 89% and 94% when using “virtual endoscopy”, and 62% and 100% when using axial 2D reconstruction. Sensitivity in relation to the diameter of colon polyps with “colon dissection”, “virtual colonoscopy”, and axial 2D‐slices was: polyps with a diameter >5.0 mm, 100%, 100%, and 71%, respectively; polyps with a diameter of between 3 and 4.9 mm, 92%, 85%, and 46%; and polyps with a diameter <3 mm, 89%, 78%, and 56%. The difference between “virtual endoscopy” and “colon dissection” in diagnosing polyps up to 4.9 mm in diameter was statistically significant. Conclusion: 3D reconstruction software “colon dissection” improves sensitivity of CT colonography compared with the endoluminal view.


2005 ◽  
Vol 119 (9) ◽  
pp. 693-698 ◽  
Author(s):  
Beom-Cho Jun ◽  
Sun-Wha Song ◽  
Ju-Eun Cho ◽  
Chan-Soon Park ◽  
Dong-Hee Lee ◽  
...  

The aim of this study was to investigate the usefulness of a three-dimensional (3D) reconstruction of computed tomography (CT) images in determining the anatomy and topographic relationship between various important structures. Using 40 ears from 20 patients with various otological diseases, a 3D reconstruction based on the image data from spiral high-resolution CT was performed by segmentation, volume-rendering and surface-rendering algorithms on a personal computer. The 3D display of the middle and inner ear structures was demonstrated in detail. Computer-assisted measurements, many of which could not be easily measured in vivo, of the reconstructed structures provided accurate anatomic details that improved the surgeon’s understanding of spatial relationships. A 3D reconstruction of temporal bone CT might be useful for education and increasing understanding of the anatomical structures of the temporal bone. However, it will be necessary to confirm the correlation between the 3D reconstructed images and histological sections through a validation study.


2019 ◽  
Vol 32 (02) ◽  
pp. 139-148 ◽  
Author(s):  
John Davies ◽  
Albert Lynch

Objective The main aim of this study was to evaluate a percutaneous method of bone alignment using a diaphyseal tibial fracture model. Materials and Methods Mid-shaft diaphyseal fractures were created in 12 large-breed canine tibiae. Interaction pins were inserted into the proximal and distal bone segments. Computed tomography scans of the fractured tibiae and pins were imported into three-dimensional (3D) modelling software and the fractures were virtually reduced. A multi-component 3D printed alignment jig was created that encompassed the pins in their aligned configuration. Orthogonal radiographs were taken after alignment jig application. Intact and post-alignment tibial lengths and joint angles were compared. Rotational alignment was subjectively evaluated. Results Post-alignment tibial lengths differed on the mediolateral and craniocaudal radiographs by an average of 1.55 and 1.43% respectively. Post-alignment mechanical medial proximal tibial angle, mechanical medial distal tibial angle and mechanical caudal proximal tibial angle had an average difference of 1.67°, 1.92° and 2.17° respectively. Differences in tibial length and joint angles were not significant (p > 0.05). Clinical Significance While in vivo evaluation is necessary, this technique to align diaphyseal fractures percutaneously using computer modelling and 3D printing is technically feasible and may facilitate the clinical use of minimally invasive osteosynthesis techniques.


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