Human CSF Movement Influenced by Vascular Low Frequency Oscillations and Respiration

Background: Cerebrospinal fluid movement (CSF) through the pathways within the central nervous system is of high significance for maintaining normal brain health and function. Low frequency hemodynamics and respiration have both been shown to independently drive CSF in humans. Here, we hypothesize that CSF movement may be driven simultaneously (and in synchrony) by both mechanisms and we study their independent and interactive effects on CSF movement using novel neck fMRI scans.Methods: Caudad CSF movement at the fourth ventricle and hemodynamics of the major neck blood vessels (internal carotid arteries and internal jugular veins) were captured from 11 young healthy volunteers using novel neck fMRI scans with simultaneous measurement of respiration. Two distinct models of CSF movement (1. Low-frequency hemodynamics and 2. Respiration) were independently investigated in corresponding frequency ranges. Possible interactions between these mechanisms were also studied using cross-frequency coupling.Results: The results from this study validated that the caudad CSF movement may be driven by both low frequency hemodynamics (0.01 Hz – 0.1 Hz) and respiration (0.2 Hz - 0.4 Hz), through different mechanisms. We show that the dynamics of brain fluids can be assessed from the neck, by studying the interrelationships between major neck blood vessels and the CSF movement at the fourth ventricle. We also demonstrate that there exists a cross-frequency interaction between two separable mechanisms.Conclusions: The human CSF system is capable of responding to multiple interacting physiological forces at the same time. This information may help inform the pathological mechanisms behind CSF movement-related disorders and facilitate new approaches to therapeutic interventions.

Investigation on application of singular value decomposition filter in element domain for extraction of ultrasonic echoes from blood cells in jugular veins

The singular value decomposition (SVD) based clutter filter is commonly applied to beamformed signals for the visualization of echo signals from flowing blood cells. In this paper, the SVD-based clutter filter is applied to signals directly acquired from ultrasonic elements before beamforming to be compared with the conventional strategy by evaluating contrast and standard deviation (SD) in the filtered images. As a result, the contrast was improved from 10.7 ± 3.6 dB to 18.3 ± 4.6 dB, and the SD was slightly improved from 3.78 ± 0.69 dB to 3.07 ± 0.74 dB in measurement of a right jugular vein.

Interrelation of cognitive functions and neural networks with blood flow velocity through the internal jugular vein in patients with chronic cerebral ischemia

Understanding age-related and functional changes in cerebral venous circulation is critical for the development of new preventive, diagnostic and therapeutic approaches to maintaining brain health in the elderly. Chronic cerebral ischemia is one of the widespread socially significant vascular diseases caused by a decrease in the level of blood circulation. To assess the role of venous outflow through the internal jugular veins in cognitive decline and neural networks in patients with chronic cerebral ischemia, 30 men and 40 women (average age 66.5 years), cognitive functions and organization of neural networks were studied at high and low levels of cerebral venous blood flow through the internal jugular veins. To assess the venous outflow, the systolic blood flow rate was measured by the internal jugular veins. A higher rate of venous outflow through internal jugular veins is associated with a more successful performance of the Luria test for verbal memory. A higher or lower blood flow rate affects the formation of neural networks of the brain. At a higher blood flow rate, the predominant areas of the resting neural networks (the passive mode network of the brain and the salient network) are localized in the frontal regions, at a low blood flow rate, the predominant neural network (frontal-parietal) is located in the left hemisphere. The state of faster and slower venous outflow forms neural networks using different neural formations that affect verbal memory. Reorganization of neural networks in this case, apparently, is the central mechanism responsible for cognitive decline in chronic cerebral ischemia (2 figs, 1 table, bibliography: 10 refs)

CS-5 A case of anaplastic pleomorphic xanthoastrocytoma in the pineal region

Tumors in the pineal gland are rare tumors that account for about 0.3% of all brain tumors and have various histological types of tumors develop with germinoma, pineocytoma, and pineoblastoma in that order. On the other hand, pleomorphic xanthoastrocytoma (PXA) is a rare tumor of less than 0.2% and frequently occurs in supratentorial cerebral surface of children and young adults.A case was a 61-year-old man whose pineal tumor was found due to visual disturbance. MRI showed a 23 mm-sized lesion with cysts and inhomogeneous enhancement in the pineal gland. Partial calcification was observed, but there was no non-communicating hydrocephalus, and no increase in HCG-β and AFP with blood sampling. A midline suboccipital craniotomy was performed in the sitting position, a head-up surgery was performed using a 4K / 3D video microscope system (ORBEYE exoscope, Olympus) by the infratentorial supracerebellar approach (ITSCA). The bridging veins and precentral cerebellar vein were dissected to expose the posterior surface of the tumor, and internal decompression was performed. For the complication of air embolism, artificial cerebrospinal fluid was sprayed, and the bilateral internal jugular veins were compressed to confirm the inflow point. There was strong adhesion to the Rt vein of Rosenthal, and the site was removed intracapsularly. Finally, subtotal resection was performed with remaining the upper part of the tumor, a blind lesion behind the Vein of Galen. Vertical gaze palsy occurred after this operation, but it gradually improved over time. Tumor showed pathologically remarkable polymorphism, poor microvascular proliferation and necrosis, but mitotic figures 4–5 / 10HPF, MIB-1 index 10%, GFAP positive, no BRAF V600E mutation. There are few reports of PXA occurring in the pineal gland, and this case is the sixth case. It is also the first report for pineal tumors using ORBEYE through ITSCA in the sitting position.

Viscera of the neck

The thyroid gland is a symmetrical H-shaped endocrine structure in the lower neck. It consists of two lobes, each extending from the oblique line of the thyroid cartilage above to the sixth tracheal ring below – united by a median isthmus covered by the anterior jugular veins. The small, (usually) paired and inconsistent parathyroid glands lie behind the lobes of the thyroid gland. They measure 6mm by 4mm by 2mm and are ordinarily four in number – two superior and two inferior. They are involved in the careful regulation of the body’s calcium levels. Both superior and inferior glands are ordinarily supplied by the inferior thyroid artery. Drainage is into the venous plexus on the anterior surface of the thyroid.

The influence of the Zoobior remedy, used in an implementation study on the health and clinical hematological status of young hens in the first laying phase

This article presents the scientific results focused on investigating the impact of the ZooBioR remedy, obtained from Spirulina platensis, tested on young hens, in the first technological phase of laying. The research was initiated on young hens, of 24 weeks old, belonging to the “Hy-Line” hybrid, bred in identical conditions of nutrition and maintenance. The hens in the experimental group received daily food supplemented with ZooBioR, in proportion of 10 mg active substance/kg feed. During the study the birds were monitored, and periodically were determined body temperature, frequency of respiratory movements. For haematological investigations, from birds was collected blood from the axial veins and, as the case may be, from the jugular veins, in standard model test tubes. It has been established that the ZooBioR remedy is well tolerated by young hens and exhibits anti-stress and adaptive properties. In addition, the tested product has a positive evolution on the hemogram, increases the natural body resistance, inducing at the end of the study a tendency of increase of platelets in blood.

Biocompatibility and patency of a novel titanium vascular anastomotic device in a pig jugular vein

This study aimed to evaluate the biocompatibility and patency of our newly developed titanium vascular anastomotic device (TVAD) in a pig jugular vein. TVAD was made of commercially pure grade 2 titanium. The patency and anastomotic time were simultaneously confirmed in an ex-vivo system developed by the authors and in vivo using pig jugular veins. Five 8-month-old pigs, with body weights of 50–60 kg, underwent anastomosis of both jugular veins using the device. Graft patency was evaluated for 12 weeks by biplane angiography and sonography. All tissue biopsy samples were analysed by histology. In all 10 cases, the anastomosis was completed in < 5 min. The vessel lumen was not damaged, and the inner vessel wall was completely endothelialised at the anastomotic site. No foreign body reactions were observed at the vessel lumen, vessels, and outer vessel walls by histopathologic analysis. Patency and absence of leakage at the anastomotic site of the follow-up period were confirmed clearly by angiography and sonography. This preliminary animal study proved that our newly developed device is a very promising tool for intima-to-intima contact anastomosis. TVAD can be used as a feasible and safe medical tool for vessel anastomosis.

In Vivo Rodent Cervicothoracic Vasculature Imaging Using Photoacoustic Computed Tomography

Mice and rats are rodent specimens commonly used in multidisciplinary research. Specifically, vasculature imaging of rodents has been widely performed in preclinical studies using various techniques, such as computed tomography, magnetic resonance imaging, and ultrasound imaging. Photoacoustic CT (PACT) is a noninvasive, nonionizing optical imaging technique derived from photoacoustic tomography and benefits from using intrinsic endogenous contrast agents to produce three-dimensional volumetric data from images. In this study, a commercial PACT device was employed to assess the cervicothoracic vasculature of mouse and rat specimens, which has rarely been examined using PACT, under two conditions with depilation and skin incision. Various blood vessels, including the common carotid artery, internal/external jugular veins, cranial vena cava, internal thoracic vein, and mammary, were identified in the acquired PACT images. The difference between the depilated and skin-incised specimens also revealed the presence of branches from certain blood vessels and specific anatomical features such as the manubrium of the sternum. This study presents detailed PACT images observing the cervicothoracic vasculature of rodent specimens and is expected to be used as a reference for various preclinical experiments on mice and rats.

Haematological parameters in relation to age, sex and body weight of free-range village chickens (Gallus gallus domesticus) in Maiduguri, Nigeria

The haematology of animals including chickens is an important indicator of their health status. It is therefore considered a useful tool in clinical diagnosis. The aim of this study was to investigate the haematological parameters of apparently healthy free range domesticated chickens as influenced by their sex, age and body weights. Blood samples taken from the jugular veins were analysed using standard haematological protocols. PCV, haemoglobin concentration, TRBC, TWBC, differential leukocyte counts, erythrocytes indices (MCV, MCHC and MCH) were measured. The study showed that there were no significant (P>0.05) age-dependent variation in all the measured haematological parameters. However, sex had effect on PCV, TRBC and heterophil count. Male chickens had higher PCV value of (34.4%) and TRBC value of (2.4 × 1012/L) as compared to female chickens with PCV value of (30.5%) and TRBC value of (2.1 × 1012/L). On the other hand, female chickens had higher mean heterophil value of (22.0 × 109/L) and MCHC value of (29.5g/dl) as compared to male chickens with mean heterophil and MCHC values of (16.8 × 109/L) and (25.7g/dl) respectively. There was significant (P<0.05) increase in PCV with increasing body weights in males but not in females. The MCHC decreased significantly (P<0.05) as the body weight increased also in males but not in females. The results of the study indicate that sex and body weights influenced normal haematological parameters of apparently healthy free range domestic chickens in the study environment. Keywords: Age, body weight, haematology, sex, village chicken.

The impact of spontaneous echo contrast on tunneled dialysis catheter patency

Background: Spontaneous echo contrast (SEC) is an ultrasonographic image of swirling blood flow resembling a dynamic, cigarette smoke-like image. It is mostly observed during the insertion of tunneled dialysis catheters (TDC) into internal jugular veins with ultrasound guidance, often different levels of SEC can be seen. The aim of this study is to investigate the impact of SEC detected during TDC insertion on the patency of the catheter. Methods: Patients who had a TDC insertion in our clinic between January 2015 and December 2020 were prospectively evaluated. The patients were grouped into five groups according to the sec level and followed. Results: A total of 226 patients were examined, among which 107 were male (47.3%). The mean age of all patients was 63.1 ± 9.5 years. SEC grade and catheter occlusion was evaluated, it was observed that higher SEC grades were correlated with faster catheter thrombosis postinsertion. During the follow-up period, it was found that, compared to the SEC 0 group, catheter thrombosis occurred 3.22 times faster in the SEC 1 group, 2.66 times faster in the SEC 2 group, 5.80 times faster in the SEC 3 group, and 26.33 times faster in the SEC 4 group. (HR: 3.22, 2.66, 5.80, 26.33, respectively). Hematological parameters were evaluated by regression analysis, it was observed that hemoglobin, fibrinogen, and platelet count were not risk factors for SEC formation and SEC grade. Conclusion: Significant relationship was found between SEC grade detected during catheter insertion and catheter thrombosis in patients undergoing hemodialysis with a TDC.