scholarly journals Identification and Detection of Botryosphaeria dothidea from Kiwifruit (Actinidia chinensis) in China

Plants ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 401
Author(s):  
Li Wang ◽  
Hui Hou ◽  
Zengqiang Zhou ◽  
Hongtao Tu ◽  
Hongbo Yuan
Keyword(s):  
Internal Transcribed Spacer ◽  
Nutritional Value ◽  
Disease Monitoring ◽  
Lamp Method ◽  
Disease Prediction ◽  
Its Sequencing ◽  
Botryosphaeria Dothidea ◽  
Monitoring And Control ◽  
Diseased Fruit ◽  
And Control

Kiwifruit is very popular among consumers due to its high nutritional value. The increasing expansion in kiwifruit cultivation has led to the spread of rot diseases. To identify the pathogens causing kiwifruit ripe rots in China, 24 isolates were isolated from the diseased fruit and wart in trees. Botryosphaeria dothidea was recognized as the pathogen causing kiwifruit ripe rot and wart in the tree through internal transcribed spacer (ITS) sequencing, pathogenicity testing, morphological and microscopic characteristics. The rapid and accurate detection of this pathogen will lead to better disease monitoring and control efforts. A loop-mediated isothermal amplification (LAMP) method was then developed to rapidly and specifically identify B. dothidea. These results offer value to further research into kiwifruit ripe rot, such as disease prediction, pathogen rapid detection, and effective disease control.

Occurrence and Detection of Carbendazim-Resistance in Botryosphaeria dothidea from apple orchards in China

Plant Disease ◽  
2021 ◽  
Author(s):  
Li Wang ◽  
Hongtao Tu ◽  
Hui Hou ◽  
Zengqiang Zhou ◽  
Hongbo Yuan ◽  
...  
Keyword(s):  
Glutamic Acid ◽  
Mycelial Growth ◽  
White Rot ◽  
Lamp Method ◽  
Botryosphaeria Dothidea ◽  
Apple Trees ◽  
Tubulin Gene ◽  
And Control ◽  
Resistance Stability ◽  
Β Tubulin

Botryosphaeria dothidea causes white rot, which is among the most devastating diseases affecting apple crops globally. In this paper, we assessed B. dothidea resistance to carbendazim by collecting samples from warts on the infected branches of apple trees or from fruits exhibiting evidence of white rot. All samples were collected from different orchards of nine provinces of China in 2018 and 2019. In total 440 B. dothidea isolates were evaluated, of which 19 isolates from three provinces were found to exhibit carbendazim-resistance. We additionally explored the fitness and resistance stability of these isolates, revealing that they were no less fit than carbendazim-sensitive isolates in terms of pathogenicity, sporulation, and mycelial growth and that the observed carbendazim resistance was stable. Sequencing of the β-tubulin gene in carbendazim-resistant isolates showed the presence of a substitution at codon 198 (GAG to GCG) that result in an alanine substitution in place of glutamic acid (E198A) in all 19 resistant isolates. A LAMP method was then developed to rapidly and specifically identify this E198A mutation. This LAMP method offers value as a tool for rapidly detecting carbendazim-resistant isolates bearing this E198A mutation, and can thus be used for the widespread monitoring of apple crops to detect and control the development of such resistance

Computers for data, control and simulation

1988 ◽  
Vol 46 ◽  
pp. 920-921
Author(s):  
David C. Joy
Keyword(s):  
Personal Computers ◽  
Monitoring And Control ◽  
Data Logging ◽  
Data Logger ◽  
Operational Conditions ◽  
Data Control ◽  
The Many ◽  
And Control

Personal computers (PCs) are a powerful resource in the EM Laboratory, both as a means of automating the monitoring and control of microscopes, and as a tool for quantifying the interpretation of data. Not only is a PC more versatile than a piece of dedicated data logging equipment, but it is also substantially cheaper. In this tutorial the practical principles of using a PC for these types of activities will be discussed.The PC can form the basis of a system to measure, display, record and store the many parameters which characterize the operational conditions of the EM. In this mode it is operating as a data logger. The necessary first step is to find a suitable source from which to measure each of the items of interest. It is usually possible to do this without having to make permanent corrections or modifications to the EM.

The magnitude and cost of groundwater monitoring and control in agriculture.

2016 ◽  
Author(s):  
Giacomo Giannoccaro ◽  
Armando Ursitti ◽  
Maurizio Prosperi
Keyword(s):  
Groundwater Monitoring ◽  
Monitoring And Control ◽  
And Control

MONITORING AND CONTROL OF THE “ANIMAL” SUBSYSTEM IN THE COMPLEX BIOTECHNICAL SYSTEM “MAN-MACHINE-ANIMAL” OF A DAIRY FARM

2020 ◽  
pp. 4-10
Author(s):  
VLADIMIR V. KIRSANOV ◽  
◽  
DMITRIY YU. PAVKIN ◽  
FEDOR E. FEDOR E. VLADIMIROV ◽  
EVGENIY А. NIKITIN ◽  
...  
Keyword(s):  
Dairy Farm ◽  
Animal Husbandry ◽  
Reproductive Organs ◽  
Monitoring And Control ◽  
Lactating Cows ◽  
Research Activities ◽  
Mutual Adaptation ◽  
Biotechnical System ◽  
Complex Project ◽  
And Control

A modern dairy farm is a complex biotechnical “man-machine-animal” system, where purposeful human activity concentrates mainly on the control of the “machine” and “animal” subsystems, thus making the whole system ergatic. Increasing the interaction effi ciency of machine subsystems with biological objects (animals) requires an in-depth study of the properties and characteristics of the latter, their behavior, adaptive and refl ex mechanisms that ensure the mutual adaptation of machine and biological subsystems. The paper considers general functionality of the “animal” subsystem, which includes lists of monitored parameters (functions) in pre-weaning, pre-lactation and lactation periods. In a similar way, functionals of the subsystems of the general musculoskeletal development of the animal’s body, respiratory and digestive organs, comfort of the habitat, development and control of the reproductive organs of lactating cows were obtained accompanied with a list of controlled functions and parameters. To carry out a set of research activities in this fi eld, FSAC VIM is planning to carry out a complex project that will increase the levels of automation, digitalization and intellectualization of animal husbandry, provide for comfortable environment, optimal rediced-impact service modes for animals and their extended productive longevity, increased quality of milk and autonomous functioning of individual local biotechnical subsystems.

The Threats of Ukraine Financial Security: Identification and Systemization

2019 ◽  
pp. 73-80
Author(s):  
Oleksandra Maslii ◽  
Andrii Maksymenko ◽  
Svitlana Onyshchenko
Keyword(s):  
Financial Stability ◽  
System Development ◽  
Financial System ◽  
Economic Security ◽  
The State ◽  
Financial Security ◽  
Monitoring And Control ◽  
Development Indicators ◽  
Security Indicators ◽  
And Control

Place of monitoring and control of risks of financial stability of the state in the system of ensuring financial security of the state was substantiated. Methods of identifying threats to Ukraine's financial security through the current and strategic analysis of financial system development indicators were considered. Tendencies of economic development of Ukraine in the context of revealing sources of threats to financial stability of the state were analyzed. Dynamic analysis of the actual values of the financial security indicators of Ukraine as a whole and its separate components had been carried out. Threats to Ukraine's financial security were identified based on comparative and trend analysis. Reasons for the critical state of debt, banking and monetary security in the financial structure and the preconditions for the emergence of systemic threats had been investigated. Systematization of risks and threats to Ukraine's financial security by its components had been carried out. Influence of systemic threats in the financial sphere on the economic security of the state was generalized. International experience of monitoring financial stability of the state was analyzed. Additional risks to the national financial system are associated with the globalization and digitization of the state financial system that are not taken into account by valid methodological recommendations for calculating the level of economic security of Ukraine were highlighted.

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