scholarly journals Both Allene Oxide Synthases Genes Are Involved in the Biosynthesis of Herbivore-Induced Jasmonic Acid and Herbivore Resistance in Rice

Plants ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 442
Author(s):  
Jiamei Zeng ◽  
Tongfang Zhang ◽  
Jiayi Huangfu ◽  
Ran Li ◽  
Yonggen Lou
Keyword(s):  
Jasmonic Acid ◽  
Brown Planthopper ◽  
Transcript Level ◽  
Stem Borer ◽  
Vital Role ◽  
Allene Oxide ◽  
Mechanical Wounding ◽  
Oxide Synthase ◽  
Phloem Feeding

Allene oxide synthase (AOS) is the second enzyme in the biosynthesis of the plant defensive hormone jasmonic acid (JA). In rice, there are two AOSs, OsAOS1 and OsAOS2. However, the role of these two AOS genes in herbivore-induced defenses in rice remains unidentified. We cloned the two rice AOS genes and observed that the transcript level of both OsAOS1 and OsAOS2 was enhanced by mechanical wounding, the infestation of the striped stem borer (SSB) (Chilo suppressalis) or brown planthopper (BPH) (Niaparvata lugens), and treatment with JA; however, OsAOS1 responded more rapidly to SSB infestation and JA treatment than did OsAOS2. The antisense expression of OsAOS1 (as-aos1) or OsAOS2 (as-aos2) decreased levels of SSB- or BPH-induced JA, which, in turn, reduced the production of SSB-induced trypsin protease inhibitor (TrypPI) and volatiles as well as the resistance of rice to SSB. In contrast, BPH preferred to feed and oviposit on wild-type (WT) plants over as-aos1 and as-aos2 plants. Moreover, the survival of BPH nymphs on as-aos1 or as-aos2 lines was significantly lower than on WT plants. The increased resistance of as-aos1 or as-aos2 plants to BPH correlated with higher levels of BPH-induced H2O2 and SA. These results indicate that OsAOS1 and OsAOS2 are both involved in herbivore-induced JA biosynthesis and play a vital role in determining the resistance of rice to chewing and phloem-feeding herbivores.

RNA interference-aided knockdown of a putative saccharopine dehydrogenase leads to abnormal ecdysis in the brown planthopper, Nilaparvata lugens (Stål) (Hemiptera: Delphacidae)

2015 ◽  
Vol 105 (4) ◽  
pp. 390-398 ◽  
Author(s):  
P.-J. Wan ◽  
L. Yang ◽  
S.-Y. Yuan ◽  
Y.-H. Tang ◽  
Q. Fu ◽  
...  
Keyword(s):  
Brown Planthopper ◽  
Transcript Level ◽  
Nilaparvata Lugens ◽  
The Body ◽  
Double Stranded Rna ◽  
Saccharopine Dehydrogenase ◽  
Body Weights ◽  
Nilaparvata Lugens Stål ◽  
Phloem Feeding ◽  
Wing Deformation

AbstractThe brown planthopper Nilaparvata lugens is a serious phloem-feeding pest of rice in China. The current study focuses on a saccharopine dehydrogenase (SDH) that catalyzes the penultimate reaction in biosynthesis of the amino acid lysine (Lys), which plays a role in insect growth and carnitine production (as a substrate). The protein, provisionally designated as NlylsSDH [a SDH derived from yeast-like symbiont (YLS) in N. lugens], had a higher transcript level in abdomens, compared with heads, wings, legs and thoraces, which agrees with YLS distribution in N. lugens. Ingestion of Nlylssdh targeted double-stranded RNA (dsNlylssdh) for 5, 10 and 15 days decreased the mRNA abundance in the hoppers by 47, 70 and 31%, respectively, comparing with those ingesting normal or dsegfp diets. Nlylssdh knockdown slightly decreased the body weights, significantly delayed the development of females, and killed approximately 30% of the nymphs. Moreover, some surviving adults showed two apparent phenotypic defects: wing deformation and nymphal cuticles remained on tips of the legs and abdomens. The brachypterours/macropterours and sex ratios (female/male) of the adults on the dsRNA diet were lowered compared with the adults on diets without dsRNA. These results suggest that Nlylssdh encodes a functional SDH protein. The adverse effect of Nlylssdh knockdown on N. lugens implies the importance of Lys in hopper development. This study provides a proof of concept example that Nlylssdh could serve as a possible dsRNA-based pesticide for planthopper control.

scholarly journals Functional Characterization of An Allene Oxide Synthase Involved in Biosynthesis of Jasmonic Acid and Its Influence on Metabolite Profiles and Ethylene Formation in Tea (Camellia sinensis) Flowers

2018 ◽  
Vol 19 (8) ◽  
pp. 2440 ◽  
Author(s):  
Qiyuan Peng ◽  
Ying Zhou ◽  
Yinyin Liao ◽  
Lanting Zeng ◽  
Xinlan Xu ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Jasmonic Acid ◽  
Camellia Sinensis ◽  
Allene Oxide ◽  
Allene Oxide Synthase ◽  
Tea Leaves ◽  
Ethylene Formation ◽  
Horticultural Crops ◽  
Metabolite Profiles ◽  
Oxide Synthase

Jasmonic acid (JA) is reportedly involved in the interaction between insects and the vegetative parts of horticultural crops; less attention has, however, been paid to its involvement in the interaction between insects and the floral parts of horticultural crops. Previously, we investigated the allene oxide synthase 2 (AOS2) gene that was found to be the only JA synthesis gene upregulated in tea (Camellia sinensis) flowers exposed to insect (Thrips hawaiiensis (Morgan)) attacks. In our present study, transient expression analysis in Nicotiana benthamiana plants confirmed that CsAOS2 functioned in JA synthesis and was located in the chloroplast membrane. In contrast to tea leaves, the metabolite profiles of tea flowers were not significantly affected by 10 h JA (2.5 mM) treatment as determined using ultra-performance liquid chromatography/quadrupole time-of-flight mass spectrometry, and gas chromatography-mass spectrometry. Moreover, JA treatment did not significantly influence ethylene formation in tea flowers. These results suggest that JA in tea flowers may have different functions from JA in tea leaves and other flowers.

scholarly journals Caterpillar-induced rice volatiles provide enemy-free space for the offspring of the brown planthopper

eLife ◽  
2020 ◽  
Vol 9 ◽  
Author(s):  
Xiaoyun Hu ◽  
Shuangli Su ◽  
Qingsong Liu ◽  
Yaoyu Jiao ◽  
Yufa Peng ◽  
...  
Keyword(s):  
Control Strategies ◽  
Brown Planthopper ◽  
Stem Borer ◽  
Egg Parasitoid ◽  
Field Cage ◽  
Parasitism Rates ◽  
Enemy Free Space ◽  
Rice Pest ◽  
Herbivore Induced Plant Volatiles

Plants typically release large quantities of volatiles in response to herbivory by insects. This benefits the plants by, for instance, attracting the natural enemies of the herbivores. We show that the brown planthopper (BPH) has cleverly turned this around by exploiting herbivore-induced plant volatiles (HIPVs) that provide safe havens for its offspring. BPH females preferentially oviposit on rice plants already infested by the rice striped stem borer (SSB), which are avoided by the egg parasitoid Anagrus nilaparvatae, the most important natural enemy of BPH. Using synthetic versions of volatiles identified from plants infested by BPH and/or SSB, we demonstrate the role of HIPVs in these interactions. Moreover, greenhouse and field cage experiments confirm the adaptiveness of the BPH oviposition strategy, resulting in 80% lower parasitism rates of its eggs. Besides revealing a novel exploitation of HIPVs, these findings may lead to novel control strategies against an exceedingly important rice pest.

scholarly journals Allene oxide synthase, allene oxide cyclase and jasmonic acid levels in Lotus japonicus nodules

PLoS ONE ◽  
2018 ◽  
Vol 13 (1) ◽  
pp. e0190884 ◽  
Author(s):  
Anna Zdyb ◽  
Marco G. Salgado ◽  
Kirill N. Demchenko ◽  
Wolfram G. Brenner ◽  
Małgorzata Płaszczyca ◽  
...  
Keyword(s):  
Jasmonic Acid ◽  
Lotus Japonicus ◽  
Allene Oxide ◽  
Allene Oxide Synthase ◽  
Allene Oxide Cyclase ◽  
Oxide Synthase

scholarly journals Inducible Overexpression of a Rice Allene Oxide Synthase Gene Increases the Endogenous Jasmonic Acid Level, PR Gene Expression, and Host Resistance to Fungal Infection

2006 ◽  
Vol 19 (10) ◽  
pp. 1127-1137 ◽  
Author(s):  
Chuansheng Mei ◽  
Min Qi ◽  
Guangyao Sheng ◽  
Yinong Yang
Keyword(s):  
Gene Expression ◽  
Jasmonic Acid ◽  
Induced Resistance ◽  
Defense Response ◽  
Cytochrome P450 Enzyme ◽  
Allene Oxide ◽  
Allene Oxide Synthase ◽  
Pathogen Infection ◽  
Rice Plants ◽  
Oxide Synthase

Many studies in dicotyledonous plants have shown that jasmonates, including jasmonic acid (JA) and methyl jasmonate, are important signal molecules involved in induced resistance to pathogen infection and insect herbivory. However, very little genetic and molecular evidence is available to demonstrate their role in host defense response of rice and other economically important monocot plants. In this study, we have shown that exogenous application of JA was able to activate defense gene expression and local induced resistance in rice seedlings against the rice blast fungus (Magnaporthe grisea). Furthermore, we have characterized a pathogen-inducible rice OsAOS2 gene (which encodes allene oxide synthase, a key enzyme in the JA biosynthetic pathway) and examined the role of endogenous JA in rice defense response through transgenic manipulation of the JA biosynthesis. Sequence analysis indicated that OsAOS2 contains four common domains of the cytochrome P450 enzyme, but does not have the signal peptide for chloroplast targeting. The basal level of OsAOS2 expression is very low in leaves but relatively high in the sheath, culm, and flower of rice plants. Interestingly, the expression of OsAOS2 in rice leaves can be induced significantly upon M. grisea infection. Transgenic rice lines carrying the OsAOS2 transgene under the control of a strong, pathogen-inducible PBZ1 promoter accumulated abundant OsAOS2 transcripts and higher levels of JA, especially after the pathogen infection. These transgenic lines also exhibited enhanced activation of pathogenesis-related (PR) genes such as PR1a, PR3, and PR5 and increased resistance to M. grisea infection. Our results suggest that JA plays a significant role in PR gene induction and blast resistance in rice plants.

scholarly journals Expression of Castanea crenata Allene Oxide Synthase in Arabidopsis Improves the Defense to Phytophthora cinnamomi

2021 ◽  
Vol 12 ◽  
Author(s):  
Susana Serrazina ◽  
Helena Machado ◽  
Rita Lourenço Costa ◽  
Paula Duque ◽  
Rui Malhó
Keyword(s):  
Jasmonic Acid ◽  
Stress Responses ◽  
Phytophthora Cinnamomi ◽  
Functional Characterization ◽  
Defense Responses ◽  
Confocal Imaging ◽  
Allene Oxide ◽  
Allene Oxide Synthase ◽  
Castanea Crenata ◽  
Oxide Synthase

Allene oxide synthase (AOS) is a key enzyme of the jasmonic acid (JA) signaling pathway. The AOS gene was previously found to be upregulated in an Asian chestnut species resistant to infection by the oomycete Phytophthora cinnamomi (Castanea crenata), while lower expression values were detected in the susceptible European chestnut (Castanea sativa). Here, we report a genetic and functional characterization of the C. crenata AOS (CcAOS) upon its heterologous gene expression in a susceptible ecotype of Arabidopsis thaliana, which contains a single AOS gene. It was found that Arabidopsis plants expressing CcAOS delay pathogen progression and exhibit more vigorous growth in its presence. They also show upregulation of jasmonic acid and salicylic acid-related genes. As in its native species, heterologous CcAOS localized to plastids, as revealed by confocal imaging of the CcAOS-eGFP fusion protein in transgenic Arabidopsis roots. This observation was confirmed upon transient expression in Nicotiana benthamiana leaf epidermal cells. To further confirm a specific role of CcAOS in the defense mechanism against the pathogen, we performed crosses between transgenic CcAOS plants and an infertile Arabidopsis AOS knockout mutant line. It was found that plants expressing CcAOS exhibit normal growth, remain infertile but are significantly more tolerant to the pathogen than wild type plants. Together, our results indicate that CcAOS is an important player in plant defense responses against oomycete infection and that its expression in susceptible varieties may be a valuable tool to mitigate biotic stress responses.

scholarly journals Expression of Castanea crenata Allene Oxide Synthase in Arabidopsis Improves the Defense to Phytophthora cinnamomi

2020 ◽  
Author(s):  
Susana Serrazina ◽  
Helena Machado ◽  
Rita Costa ◽  
Paula Duque ◽  
Rui Malhó
Keyword(s):  
Arabidopsis Thaliana ◽  
Jasmonic Acid ◽  
Stress Responses ◽  
Phytophthora Cinnamomi ◽  
Defense Responses ◽  
Confocal Imaging ◽  
Allene Oxide ◽  
Allene Oxide Synthase ◽  
Castanea Crenata ◽  
Oxide Synthase

ABSTRACTAllene oxide synthase (AOS) is a key enzyme of the jasmonic acid (JA) signaling pathway. The AOS gene was previously found to be upregulated in an Asian chestnut species resistant to infection by the oomycete Phytophthora cinnamomi (Castanea crenata), while lower expression values were detected in the susceptible European chestnut (Castanea sativa). Here, we report a genetic and functional characterization of the C. crenata AOS (CcAOS) upon its heterologous gene expression in a susceptible ecotype of Arabidopsis thaliana, which contains a single AOS gene. It was found that Arabidopsis plants expressing CcAOS delay pathogen progression and exhibit more vigorous growth in its presence. They also show upregulation of jasmonic acid and salicylic acid-related genes. As in its native species, heterologous CcAOS localized to plastids, as revealed by confocal imaging of the CcAOS-eGFP fusion protein in transgenic Arabidopsis roots. This observation was confirmed upon transient expression in Nicotiana benthamiana leaf epidermal cells. To further confirm a specific role of CcAOS in the defense mechanism against the pathogen, we performed crosses between transgenic CcAOS plants and an infertile Arabidopsis AOS knockout mutant line. It was found that plants expressing CcAOS exhibit normal growth, remain infertile but are significantly more tolerant to the pathogen than wild type plants.Together, our results indicate that CcAOS is an important player in plant defense responses against oomycete infection and that its expression in susceptible varieties may be a valuable tool to mitigate biotic stress responses.One-sentence summaryHeterologous expression of the Castanea crenata allene oxide synthase gene in Arabidopsis thaliana improves the defense response to the pathogen Phytophthora cinnamomi.

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