scholarly journals Integrated Approach for Species Identification and Quality Analysis for Labisia pumila Using DNA Barcoding and HPLC

Plants ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 717
Author(s):  
Auni Aqilah Ahmad Tarmizi ◽  
Alina Wagiran ◽  
Faezah Mohd Salleh ◽  
Lee Suan Chua ◽  
Farah Izana Abdullah ◽  
...  
Keyword(s):  
Dna Barcoding ◽  
Dna Barcode ◽  
Integrated Approach ◽  
Quality Analysis ◽  
Integral Approach ◽  
Medicinal Products ◽  
Herbal Products ◽  
Herbal Medicinal Products ◽  
Herbal Medicinal ◽  
Labisia Pumila

Labisia pumila is a precious herb in Southeast Asia that is traditionally used as a health supplement and has been extensively commercialized due to its claimed therapeutic properties in boosting a healthy female reproductive system. Indigenous people used these plants by boiling the leaves; however, in recent years it has been marketed as powdered or capsuled products. Accordingly, accuracy in determination of the authenticity of these modern herbal products has faced great challenges. Lack of authenticity is a public health risk because incorrectly used herbal species can cause adverse effects. Hence, any measures that may aid product authentication would be beneficial. Given the widespread use of Labisia herbal products, the current study focuses on authenticity testing via an integral approach of DNA barcoding and qualitative analysis using HPLC. This study successfully generated DNA reference barcodes (ITS2 and rbcL) for L.pumila var. alata and pumila. The DNA barcode that was generated was then used to identify species of Labisia pumila in herbal medicinal products, while HPLC was utilized to determine their quality. The findings through the synergistic approach (DNA barcode and HPLC) implemented in this study indicate the importance of both methods in providing the strong evidence required for the identification of true species and to examine the authenticity of such herbal medicinal products.

DNA Barcoding of Philippine Herbal Medicinal Products

2016 ◽  
Vol 99 (6) ◽  
pp. 1479-1489 ◽  
Author(s):  
Ronniel D Pedales ◽  
Amor M Damatac ◽  
Carlo A Limbo ◽  
Cielo Mae D Marquez ◽  
Anna Isabel B Navarro ◽  
...  
Keyword(s):  
Dna Barcoding ◽  
Government Regulation ◽  
Quality Data ◽  
Reference Database ◽  
Its2 Region ◽  
Medicinal Products ◽  
Herbal Products ◽  
Herbal Medicinal Products ◽  
Herbal Medicinal ◽  
The Government

Abstract The Philippine government established the Traditional and Alternative Medicine Act in 1997 to promote traditionally used herbal products and to provide an effective yet affordable alternative to conventional medicines. However, government regulation of herbal medicinal products (HMPs) is not stringent, relying only on submitted quality data from the manufacturer. In this study we validated the taxonomic identity of 26 plant samples contained within 22 HMPs, each produced by different local manufacturers, through DNA barcoding of the nuclear internal transcribed spacer-2 (ITS2) region. We recovered 19 ITS2 barcodes from 26 samples. These were compared to sequences in GenBank using MEGABLAST, but ambiguous results (similar max scores for different species) were phylogenetically analyzed. Twelve of the 19 samples matched the indicated species on the product label, three were equivocal in specific identity but were placed in the expected genus, and four other samples from three manufacturers contained contamination and/or substitution. GenBank's reference database was at times problematic because some sequences were lacking or were misidentified, but the database was still useful. Overall, ITS2 barcoding was successful in authenticating the HMPs, and it is recommended during the premarket evaluation process so as to obtain a certificate of registration from the government. The government should also develop a comprehensive database of barcodes for Philippine plants, and should prioritize the development of the traditional pharmacopeia because many locally produced HMPs are not indigenous.

scholarly journals Risk of Fungi Associated with Aflatoxin and Fumonisin in Medicinal Herbal Products in the Kenyan Market

2017 ◽  
Vol 2017 ◽  
pp. 1-6 ◽  
Author(s):  
Lucia Keter ◽  
Richard Too ◽  
Nicholas Mwikwabe ◽  
Charles Mutai ◽  
Jennifer Orwa ◽  
...  
Keyword(s):  
Quality Improvement ◽  
Experimental Design ◽  
Medical Research ◽  
Microbial Quality ◽  
Medicinal Products ◽  
Herbal Products ◽  
Herbal Medicinal Products ◽  
Toxigenic Fungi ◽  
Herbal Medicinal ◽  
Fungal Contaminants

Utilization of herbal products is a major concern due to the possibility of contamination by toxigenic fungi that are mycotoxin producers such as Aspergillus species during processing and packaging. Research was carried out to determine the presence of aflatoxins and fumonisins in herbal medicinal products sold in Eldoret and Mombasa towns in Kenya. The study employed both exploratory and laboratory experimental design. The herbal products were purchased from the market and transported to Kenya Medical Research Institute for processing and analysis. Fungal contaminants were determined according to Pharmacopoeia specifications. The toxins were quantified using ELISA based technique. The genus Aspergillus was the most dominant followed by Penicillium. Fungal counts ranged between 1 CFU/g and >1000 cfu/g. Analysis of variance showed that the rate of fungal contaminants for Eldoret and Mombasa samples had significant association (p≤0.001). Aflatoxin levels ranged from 1 to 24 ppb, while fumonisin levels ranged from 1 to >20 ppb. Only 31% of samples met the standards for microbial limits as specified in Pharmacopoeia. There is need for product microbial quality improvement through proper harvesting, processing, storage, and marketing. It is recommended that a policy be enacted to enable regulation of herbal products in Kenya.

scholarly journals Review: DNA Barcoding and Chromatography Fingerprints for the Authentication of Botanicals in Herbal Medicinal Products

2017 ◽  
Vol 2017 ◽  
pp. 1-28 ◽  
Author(s):  
Bashir Mohammed Abubakar ◽  
Faezah Mohd Salleh ◽  
Mohd Shahir Shamsir Omar ◽  
Alina Wagiran
Keyword(s):  
Quality Control ◽  
Dna Barcoding ◽  
Rapid Identification ◽  
Efficacy And Safety ◽  
Medicinal Products ◽  
Chromatographic Fingerprint ◽  
Closely Related Species ◽  
Herbal Medicinal Products ◽  
Herbal Medicinal ◽  
Process Product

In the last two decades, there has been a tremendous increase in the global use of herbal medicinal products (HMPs) due to their claimed health benefits. This has led to increase in their demand and consequently, also, resulted in massive adulteration. This is due to the fact that most of the traditional methods cannot identify closely related species in a process product form. Therefore the urgent need for simple and rapid identification methods resulted in the discovery of a novel technique. DNA barcoding is a process that uses short DNA sequence from the standard genome for species identification. This technique is reliable and is not affected by external factors such as climates, age, or plant part. The difficulties in isolation of DNA of high quality in addition to other factors are among the challenges encountered using the DNA barcoding in the authentication of HMP. These limitations indicated that using DNA barcoding alone may ineffectively authenticate the HMP. Therefore, the combination of DNA barcoding with chromatographic fingerprint, a popular and generally accepted technique for the assessment and quality control of HMP, will offer an efficient solution to effectively evaluate the authenticity and quality consistency of HMP. Detailed and quality information about the main composition of the HMPs will help to ascertain their efficacy and safety as these are very important for quality control.

scholarly journals Identification of herbal products used by families in the campus of Darussalam Gontor University

2021 ◽  
pp. 31-35
Author(s):  
Amal Fadholah ◽  
Solikah Ana Istikomah ◽  
Cania Sofyan Islamanda ◽  
Evi Rohana Ma’rufi Jannah
Keyword(s):  
Raw Materials ◽  
Rapid Assessment ◽  
Product Safety ◽  
Assessment Procedure ◽  
Medicinal Products ◽  
Herbal Products ◽  
Herbal Medicinal Products ◽  
Herbal Medicine Product ◽  
Herbal Medicinal ◽  
Medicine Product

Background: The development and the use of herbal medicinal products is increasing in Indonesia. Aim: To identify the safety of herbal medicine product used by families of Darussalam Gontor University (UNIDA) Gontor. Method: This research was conducted to identify herbal products that are used based on features, functions, and benefits. The method used was Rapid Assessment Procedure (RAP) with a qualitative approach. Results: The results showed there were 100 products used by 72 respondents. The level of product safety used based on raw materials was 96% and the efficacy claimed was 76%. The most type of product used was jamu (Indonesian indigenous traditional medicine) (94.7%). The range of understanding level of of respondents based on features and benefits was between good and very good. Conclusion: The use of herbal products by respondents can be viewed from the aspect of product safety levels based on raw materials (96%) and efficacy claims (76%). This was still classified as safe.

scholarly journals DNA metabarcoding to unravel plant species composition in selected herbal medicines on the National List of Essential Medicines (NLEM) of Thailand

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Santhosh Kumar J. Urumarudappa ◽  
Chayapol Tungphatthong ◽  
Pinidphon Prombutara ◽  
Suchada Sukrong
Keyword(s):  
Species Composition ◽  
Plant Species ◽  
Herbal Medicines ◽  
Essential Medicines ◽  
Medicinal Products ◽  
Herbal Products ◽  
Herbal Medicinal Products ◽  
Traditional Medicines ◽  
Dna Metabarcoding ◽  
Herbal Medicinal

Abstract Traditional medicines are widely traded across the globe and have received considerable attention in the recent past, with expectations of heightened demand in the future. However, there are increasing global concerns over admixture, which can affect the quality, safety, and efficacy of herbal medicinal products. In this study, we aimed to use DNA metabarcoding to identify 39 Thai herbal products on the Thai National List of Essential Medicines (NLEM) and assess species composition and admixture. Among the products, 24 samples were in-house-prepared formulations, and 15 samples were registered formulations. In our study, DNA metabarcoding analysis using ITS2 and rbcL barcode regions were employed to identify herbal ingredients mentioned in the products. The nuclear region, ITS2, was able to identify herbal ingredients in the products at the genus- and family-levels in 55% and 63% of cases, respectively. The chloroplast gene, rbcL, enabled genus- and family-level identifications in 58% and 73% of cases, respectively. In addition, plant species were detected in larger numbers (Family identified, absolute %) in registered herbal products than in in-house-prepared formulations. The level of fidelity increases concerns about the reliability of the products. This study highlights that DNA metabarcoding is a useful analytical tool when combined with advanced chemical techniques for the identification of plant species in highly processed, multi-ingredient herbal products.

scholarly journals CURRENT REQUIREMENTS FOR THE QUALITY OF HERBAL MEDICINAL PRODUCTS

2018 ◽  
Vol 8 (3) ◽  
pp. 170-178 ◽  
Author(s):  
E. I. Sakanyan ◽  
E. L. Kovaleva ◽  
L. N. Frolova ◽  
V. V. Shelestova
Keyword(s):  
Russian Federation ◽  
Herbal Medicines ◽  
The State ◽  
Arsenic Content ◽  
Medicinal Products ◽  
Herbal Products ◽  
Herbal Medicinal Products ◽  
The Russian Federation ◽  
Herbal Medicinal

The article provides the results of a comparative analysis of requirements laid out in the State Pharmacopoeia of the USSR, XI ed. (SPh XI) and the State Pharmacopoeia of the Russian Federation, XIII ed. (SPh XIII) concerning  quality evaluation and standardization of herbal substances, herbal medicines and other herbal products. The article discusses requirements  for the criteria of herbal substances and herbal medicines quality control described in general chapters and monographs that were previously in force and that have recently been adopted. The article mentions new herbal substances that were included into the State Pharmacopoeia of the Russian Federation for the first time as well as herbal substances included into the State Pharmacopoeia of the USSR, X ed., not included into SPh XI, but included into SPh XIII. The article analyses the requirements described in the new general chapters that were recently included into SPh XIII: “Herbal substances. Herbal preparations”, “Determination of heavy metals and arsenic content in herbal substances and herbal medicinal  products”, “Determination of water absorption  factor for herbal substances”,  “Buds”, “Cut-pressed granules”. The article suggests ways to improve methods of analysis of herbal substances and herbal medicinal products in view of recent advances in pharmacopoeial analysis and the increasing requirements for its quality.

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