scholarly journals Chloroplastic SaNADP-ME4 of C3–C4 Woody Desert Species Salsola laricifolia Confers Drought and Salt Stress Resistance to Arabidopsis

Plants ◽  
2021 ◽  
Vol 10 (9) ◽  
pp. 1827
Author(s):  
Zhibin Wen ◽  
Yulan Wang ◽  
Chunlan Xia ◽  
Yuhui Zhang ◽  
Hongxiang Zhang
Keyword(s):  
Salt Stress ◽  
Stress Resistance ◽  
Photochemical Efficiency ◽  
Confocal Imaging ◽  
Pcr Analysis ◽  
Bivalent Cation ◽  
Chlorophyll Contents ◽  
Desert Species ◽  
Drought And Salt Stress ◽  
Salt Stress Resistance

The NADP-malic enzyme (NADP-ME) catalyzes the reversible decarboxylation of L-malate to produce pyruvate, CO2, and NADPH in the presence of a bivalent cation. In addition, this enzyme plays crucial roles in plant developmental and environment responses, especially for the plastidic isoform. However, this isoform is less studied in C3–C4 intermediate species under drought and salt stresses than in C3 and C4 species. In the present study, we characterized SaNADP-ME4 from the intermediate woody desert species Salsola laricifolia. SaNADP-ME4 encoded a protein of 646 amino acids, which was found to be located in the chloroplasts based on confocal imaging. Quantitative real-time PCR analysis showed that SaNADP-ME4 was highly expressed in leaves, followed by stems and roots, and SaNADP-ME4 expression was improved and reached its maximum under the 200 mm mannitol and 100 mm NaCl treatments, respectively. Arabidopsis overexpressing SaNADP-ME4 showed increased root length and fresh weight under mannitol and salt stress conditions at the seedling stage. In the adult stage, SaNADP-ME4 could alleviate the decreased in chlorophyll contents and PSII photochemical efficiency, as well as improve the expression of superoxide dismutase, peroxidase, and pyrroline-5-carboxylate synthase genes to enhance reactive oxygen species scavenging capability and proline levels. Our results suggest that SaNADP-ME4 overexpression in Arabidopsis increases drought and salt stress resistance.

scholarly journals Overexpression of AtABCG36 improves drought and salt stress resistance in Arabidopsis

2010 ◽  
Vol 139 (2) ◽  
pp. 170-180 ◽  
Author(s):  
Do-Young Kim ◽  
Jun-Young Jin ◽  
Santiago Alejandro ◽  
Enrico Martinoia ◽  
Youngsook Lee
Keyword(s):  
Salt Stress ◽  
Stress Resistance ◽  
Drought And Salt Stress ◽  
Salt Stress Resistance

scholarly journals HKT transporters mediate salt stress resistance in plants: from structure and function to the field

2015 ◽  
Vol 32 ◽  
pp. 113-120 ◽  
Author(s):  
Shin Hamamoto ◽  
Tomoaki Horie ◽  
Felix Hauser ◽  
Ulrich Deinlein ◽  
Julian I Schroeder ◽  
...  
Keyword(s):  
Salt Stress ◽  
Stress Resistance ◽  
Structure And Function ◽  
And Function ◽  
Hkt Transporters ◽  
Salt Stress Resistance

scholarly journals AtERF60 negatively regulates ABR1 to modulate salt, drought, and basal resistance in Arabidopsis

2021 ◽  
Author(s):  
Gajendra Singh Jeena ◽  
Ujjal Jyoti Phukan ◽  
Neeti Singh ◽  
Ashutosh Joshi ◽  
Alok Pandey ◽  
...  
Keyword(s):  
Abscisic Acid ◽  
Salt Stress ◽  
Stress Responses ◽  
Biotic Stress ◽  
Bacterial Pathogen ◽  
Pcr Analysis ◽  
Cis Elements ◽  
Abiotic And Biotic Stress ◽  
Basal Resistance ◽  
Drought And Salt Stress

ABSCISIC ACID REPRESSOR-1 (ABR1), an APETALA2 (AP2) domain containing transcription factor (TF) contribute important function against variety of external cues. Here, we report an AP2/ERF TF, AtERF60 that serves as an important regulator of ABR1 gene. AtERF60 is induced in response to drought, salt, abscisic acid (ABA), salicylic acid (SA), and bacterial pathogen PstDC3000 infection. AtERF60 interacts with DEHYDRATION RESPONSE ELEMENTS (DRE1/2) and GCC box indicating its ability to regulate multiple responses. Overexpression of AtERF60 results in the drought and salt stress tolerant phenotype in both seedling and mature Arabidopsis plants in comparison with the wild type (WT-Col). However, mutation in AtERF60 showed hyperactive response against drought and salt stress in comparison with its overexpression and WT. Microarray and qRT-PCR analysis of overexpression and mutant lines indicated that AtERF60 regulates both abiotic and biotic stress inducible genes. One of the differentially expressing transcripts was ABR1 and we found that AtERF60 interacts with the DRE cis-elements present in the ABR1 promoter. The mutation in AtERF60 showed ABA hypersensitive response, increased ABA content, and reduced susceptibility to PstDC3000. Altogether, we conclude that AtERF60 represses ABR1 transcript by binding with the DRE cis-elements and modulates both abiotic and biotic stress responses in Arabidopsis.

scholarly journals Identification, Characterization and Expression Analysis Reveal the Potential Function of Annexin Genes in Response to Abiotic stress, Calcium and Hormones in Cassava (Manihot esculeta Crantz)

2019 ◽  
Author(s):  
Ruimei Li ◽  
Yuqing Wang ◽  
Yangjiao Zhou ◽  
Tingting Qiu ◽  
Yu Song ◽  
...  
Keyword(s):  
Salt Stress ◽  
Abiotic Stress ◽  
Expression Patterns ◽  
Membrane Lipid ◽  
Pcr Analysis ◽  
Abiotic Stress Response ◽  
Promoter Regions ◽  
Manihot Esculenta Crantz ◽  
Diverse Range ◽  
Drought And Salt Stress

Abstract Background The calcium (Ca2+)-dependent phospholipid binding protein annexin gene family, which is known to be related to membrane lipid and cytoskeletal components, is involved in a diverse range of biological functions. However, in cassava (Manihot esculenta Crantz), no studies focusing on the roles of annexin genes in response to abiotic stresses, calcium, and hormones have been informed. Results 12 annexin genes were found and assigned to eight chromosomes in the cassava genome. All of the MeAnns contain a typical annexin domain with four 70-amino acid repeats. The MeAnns are classified into six groups in the phylogenetic tree. In their promoter regions, MeAnns possess at least 3 hormone response-related cis-elements and 1 abiotic stress response-related cis-acting element. MeAnn1, MeAnn2 and MeAnn5 exhibit very high levels of expression in each tested organs or tissues. By contrast, MeAnn12 exhibits very low levels in all the tested organs or tissues. qRT-PCR analysis indicates that both MeAnn5 and MeAnn9 have significantly high expression in leaves after cold, drought, and salt treatments and are highly responsive to CaCl2, GA and JA treatments. MeAnn2 and MeAnn10 are significantly downregulated in leaves by cold, drought and salt stress and negatively respond to CaCl2, GA and JA. The expression patterns of MeAnns under cold, drought, and salt stress are irregular in shoots. In roots, MeAnn1 and MeAnn9 are downregulated by cold, CaCl2 and JA treatments, while their other gene expression patterns are irregular. Conclutions In this study, we identified annexin genes in cassava and our expression profiling analysis demonstrated that cassava annexin genes responded to multiple stresses. Our results laid the foundation for further study of the function of cassava anesxin genes and provided an entry point for understanding the response mechanism of cassava to abiotic stress.

Response on photosynthetic pigments to drought and salt stress in some desert species

1992 ◽  
Vol 103 (7-8) ◽  
pp. 573-577 ◽  
Author(s):  
M. A. Elhaak ◽  
M. M. Migahid ◽  
K. Wegmann
Keyword(s):  
Salt Stress ◽  
Photosynthetic Pigments ◽  
Desert Species ◽  
Drought And Salt Stress
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