scholarly journals In Vitro Anti-Epstein Barr Virus Activity of Olea europaea L. Leaf Extracts

Plants ◽  
2021 ◽  
Vol 10 (11) ◽  
pp. 2445
Author(s):  
Ichrak Ben-Amor ◽  
Bochra Gargouri ◽  
Hamadi Attia ◽  
Khaoula Tlili ◽  
Imen Kallel ◽  
...  

Olea europaea L. var. sativa (OESA) preparations are widely used in traditional medicine in the Mediterranean region to prevent and treat different diseases. In this research, olive extracts derived from the leaves of the OESA tree have been screened for antioxidant activity by two methods: the DPPH free radical scavenging assay (DPPH) and the Ferric reducing antioxidant power (FRAP) assay. The DPPH assay showed that OESA possesses a stronger antioxidant activity (84%) at 1 mg/mL while the FRAP method showed a strong metal ion chelating activity (90%) at 1 mg/mL. The low IC50 values, obtained by two different methods, implies that OESA has a noticeable effect on scavenging free radicals comparable to standards. During EBV infection, the free radicals increased triggering lipid oxidation. Therefore, the monitoring of the secondary lipid peroxidation products was done by measuring malonaldehyde (MDA) and conjugated dienes (DC). The simultaneous treatment of Raji cells with OESA and TPA, as an inductorof the lytic cycle, generated a significant decrease in MDA levels and DC (p < 0.05). Besides, Raji cells simultaneously exposed to TPA and OESA exhibited a percentage of EBV-positive fluorescence cells lower than TPA treated cells (**** p < 0.0001). This suggests that OESA treatment has a protective effect against EBV lytic cycle induction.

2020 ◽  
Vol 16 (6) ◽  
pp. 912-918
Author(s):  
Javad Tavakoli ◽  
Samaneh Hashemkhani

Background: Zard variety is a popular olive variety widely cultivated in different regions of Iran. Despite the extensive cultivation of this olive cultivar, little research has been carried out on its various properties. Thus, chemical properties, qualitative parameters and antioxidant activity of Olea europaea Linn cultivar Zard oil cultivated in three different regions including Roudbar, Shiraz and Dezful could be an interesting topic for research. Methods: After oil extraction of different olive samples, their different characteristics were compared. For this purpose, fatty acid composition, unsaponifiable matters, antioxidant compounds, qualitative parameters (acid value, peroxide value, carbonyl value, total polar compounds) Antioxidant activity assay (DPPH radical-scavenging assay and ferric reducing-antioxidant power (FRAP)) and oxidative stability index were measured. Results: Palmitic acid, oleic acid and linoleic acid contents in Zard variety cultivated in Roudbar (ZR), Zard variety cultivated in Shiraz (ZS) and Zard variety cultivated in Dezful (ZD) oils were 13.1, 15.2, 13.2; 72, 60.4, 68.8; 9.1, 17 and 12.2; respectively. The highest phenolic and tocopherol content was observed in ZD oil (90.4, and 160.2 mg/kg), followed by ZR (73.3, and 121.2 mg/kg) and ZS (52.2 and 67.5 mg/kg) oils. Evaluation of qualitative parameters in the oils revealed that they have suitable quality. Antioxidant activity assay indicated that ZD oil had the highest antioxidant activity, followed by ZR and ZS oils. Conclusion: Results of this investigation indicated that there was a direct relation between antioxidant activity and antioxidant content. Also, climatic difference in cultivation regions was a reason of significant difference in the properties of ZR, ZS and ZD oils.


2018 ◽  
Vol 55 (11) ◽  
pp. 4600-4607 ◽  
Author(s):  
Saeed Ghasemi ◽  
Diba Eghbali Koohi ◽  
Mohammad Sadegh Bakhshi Emmamzadehhashemi ◽  
Shahriyar Shahbazi Khamas ◽  
Mohammad Moazen ◽  
...  

2011 ◽  
Vol 124 (1) ◽  
pp. 36-41 ◽  
Author(s):  
Mercedes Pérez-Bonilla ◽  
Sofía Salido ◽  
Teris A. van Beek ◽  
Pieter de Waard ◽  
Pablo J. Linares-Palomino ◽  
...  

2016 ◽  
Vol 2016 ◽  
pp. 1-8 ◽  
Author(s):  
Kristina Bljajić ◽  
Nina Šoštarić ◽  
Roberta Petlevski ◽  
Lovorka Vujić ◽  
Andrea Brajković ◽  
...  

B. pendulaleaf is a common ingredient in traditional herbal combinations for treatment of diabetes in southeastern Europe. Present study investigatedB. pendulaethanolic and aqueous extract as inhibitors of carbohydrate hydrolyzing enzymes, as well as their ability to restore glutathione concentration in Hep G2 cells subjected to glucose-induced oxidative stress. Phytochemical analysis revealed presence of rutin and other quercetin derivatives, as well as chlorogenic acid. In general, ethanolic extract was richer in phenolic substances than the aqueous extract. Furthermore, a comprehensive analysis of antioxidant activity of two extracts (determined by DPPH and ABTS radical scavenging activity, total antioxidant activity, and chelating activity as well as ferric-reducing antioxidant power) has shown that ethanolic extract was better radical scavenger and metal ion reductant. In addition, ethanolic extract effectively increased cellular glutathione levels caused by hyperglycemia and inhibitedα-glucosidase with the activity comparable to that of acarbose. Therefore,in vitroresearch usingB. pendulaplant extracts has confirmed their antidiabetic properties.


2000 ◽  
Vol 68 (4) ◽  
pp. 457-462 ◽  
Author(s):  
O Benavente-Garcı́a ◽  
J Castillo ◽  
J Lorente ◽  
A Ortuño ◽  
J.A Del Rio

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