Chemical Properties, Qualitative Parameters and Antioxidant Activity of Virgin Oil Obtained from Olea europaea Linn cultivar Zard Cultivated in Three Different Regions of Iran (Roudbar, Shiraz and Dezful)

2020 ◽  
Vol 16 (6) ◽  
pp. 912-918
Author(s):  
Javad Tavakoli ◽  
Samaneh Hashemkhani
Keyword(s):  
Antioxidant Activity ◽  
Olea Europaea ◽  
Radical Scavenging ◽  
Chemical Properties ◽  
Antioxidant Compounds ◽  
Activity Assay ◽  
Antioxidant Power ◽  
Olive Variety ◽  
Significant Difference ◽  
Olea Europaéa

Background: Zard variety is a popular olive variety widely cultivated in different regions of Iran. Despite the extensive cultivation of this olive cultivar, little research has been carried out on its various properties. Thus, chemical properties, qualitative parameters and antioxidant activity of Olea europaea Linn cultivar Zard oil cultivated in three different regions including Roudbar, Shiraz and Dezful could be an interesting topic for research. Methods: After oil extraction of different olive samples, their different characteristics were compared. For this purpose, fatty acid composition, unsaponifiable matters, antioxidant compounds, qualitative parameters (acid value, peroxide value, carbonyl value, total polar compounds) Antioxidant activity assay (DPPH radical-scavenging assay and ferric reducing-antioxidant power (FRAP)) and oxidative stability index were measured. Results: Palmitic acid, oleic acid and linoleic acid contents in Zard variety cultivated in Roudbar (ZR), Zard variety cultivated in Shiraz (ZS) and Zard variety cultivated in Dezful (ZD) oils were 13.1, 15.2, 13.2; 72, 60.4, 68.8; 9.1, 17 and 12.2; respectively. The highest phenolic and tocopherol content was observed in ZD oil (90.4, and 160.2 mg/kg), followed by ZR (73.3, and 121.2 mg/kg) and ZS (52.2 and 67.5 mg/kg) oils. Evaluation of qualitative parameters in the oils revealed that they have suitable quality. Antioxidant activity assay indicated that ZD oil had the highest antioxidant activity, followed by ZR and ZS oils. Conclusion: Results of this investigation indicated that there was a direct relation between antioxidant activity and antioxidant content. Also, climatic difference in cultivation regions was a reason of significant difference in the properties of ZR, ZS and ZD oils.

scholarly journals In Vitro Anti-Epstein Barr Virus Activity of Olea europaea L. Leaf Extracts

Plants ◽  
2021 ◽  
Vol 10 (11) ◽  
pp. 2445
Author(s):  
Ichrak Ben-Amor ◽  
Bochra Gargouri ◽  
Hamadi Attia ◽  
Khaoula Tlili ◽  
Imen Kallel ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Free Radicals ◽  
Olea Europaea ◽  
Metal Ion ◽  
Radical Scavenging ◽  
Lytic Cycle ◽  
Antioxidant Power ◽  
Raji Cells ◽  
Olea Europaea L ◽  
Olea Europaéa

Olea europaea L. var. sativa (OESA) preparations are widely used in traditional medicine in the Mediterranean region to prevent and treat different diseases. In this research, olive extracts derived from the leaves of the OESA tree have been screened for antioxidant activity by two methods: the DPPH free radical scavenging assay (DPPH) and the Ferric reducing antioxidant power (FRAP) assay. The DPPH assay showed that OESA possesses a stronger antioxidant activity (84%) at 1 mg/mL while the FRAP method showed a strong metal ion chelating activity (90%) at 1 mg/mL. The low IC50 values, obtained by two different methods, implies that OESA has a noticeable effect on scavenging free radicals comparable to standards. During EBV infection, the free radicals increased triggering lipid oxidation. Therefore, the monitoring of the secondary lipid peroxidation products was done by measuring malonaldehyde (MDA) and conjugated dienes (DC). The simultaneous treatment of Raji cells with OESA and TPA, as an inductorof the lytic cycle, generated a significant decrease in MDA levels and DC (p < 0.05). Besides, Raji cells simultaneously exposed to TPA and OESA exhibited a percentage of EBV-positive fluorescence cells lower than TPA treated cells (**** p < 0.0001). This suggests that OESA treatment has a protective effect against EBV lytic cycle induction.

Essential Oil and Hydrosol Extract Chemical Composition, Phytochemicals of Algerian Rhus pentaphylla Desf. and its Antioxidant Activity

2020 ◽  
Vol 16 (5) ◽  
pp. 627-638
Author(s):  
Nassima Benmansour ◽  
Asma Allal ◽  
Chaouki Selles ◽  
Boufeldja Tabti ◽  
Alain Muselli
Keyword(s):  
Antioxidant Activity ◽  
Chemical Composition ◽  
Essential Oil ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Total Phenolic ◽  
Antioxidant Compounds ◽  
Chemical Compositions ◽  
Antioxidant Power ◽  
Promising Source

Background: Rhus pentaphylla Desf. (Anacardiaceae) widely grown in Algeria, is an underutilized plant and remains poorly studied. To investigate for the first time, the chemical composition profile of essential oil and hydrosol extract; besides Total Phenolic (TPC), Flavonoid (TFC) and Condensed Tannins Contents (CTC) of fruits, leaves and roots, the fatty acid composition was assessed. The radical scavenging activity of R. pentaphylla extracts was then evaluated. Methods: Essential oil and hydrosol extract were isolated respectively by hydrodistillation and liquidliquid- extraction from the aerial parts of R. pentaphylla. The antioxidant activity was evaluated using 2,2-Diphenyl-1-Picrylhydrazyl (DPPH) radical and ferric reducing antioxidant power (FRAP). Chemical compositions of various extracts were investigated using Gas Chromatography (GC) and GC-Mass Spectrometry (GC-MS). Results: The results of GC and GC-MS analysis revealed 83 components in essential oil and 75 components in hydrosol extract representing respectively 96.9% and 92.5% of the total extract composition. The main constituents were hexadecanoic acid (31.5%) followed by spathulenol (14.9%) in the oil while the higher amount present in the hydrosol extract was of spathulenol (14.2%). Otherwise, 13 and 18 fatty acids were identified in roots and fruits respectively. The highest levels of TPC and CTC were found in roots extracts while the highest quantity of TFC has been recorded in the leaves extract. Besides, hydrosol extract was able to scavenge DPPH and FRAP free radicals more efficiently than essential oil. Conclusion: R. pentaphylla contains bioactive substances that could be used as a new promising source of antioxidant compounds in different applications.

scholarly journals Influence of Packaging Material on Polyphenol Content and Antioxidant Activity in Some Commercial Beers

Processes ◽  
2021 ◽  
Vol 9 (4) ◽  
pp. 620
Author(s):  
Mihaela Mirela Bratu ◽  
Semaghiul Birghila ◽  
Antoanela Popescu ◽  
Bogdan-Stefan Negreanu-Pirjol ◽  
Marius Radu ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Polyphenol Content ◽  
Total Polyphenol ◽  
Antioxidant Power ◽  
Phenolic Contents ◽  
Significant Difference ◽  
Ferric Reducing Antioxidant Power ◽  
Lager Beer

Using two methods (ferric reducing antioxidant power and radical scavenging activity), the total polyphenol content (Folin–Ciocalteu reagent) and polyphenol patterns (HPLC) in 10 commercial lager beer brands produced in Romania was determined. Samples bottled in glass, plastic and aluminium packages were analysed for each brand when available. Results have indicated considerable variations in the total and individual phenolic contents as well as antioxidant activity across beer brands. Gallic and feulic acids were the dominant phenolic compounds identified samples. The statistical analysis indicates a significant difference between the levels of antioxidant activity detected using DPPH and FRAP, for all three types of material considered. The statistical test ANOVA indicates that the polyphenol concentration is similar across all types of material.

scholarly journals Metabolomic Profiling of Antioxidant Compounds in Five Vachellia Species

Molecules ◽  
2021 ◽  
Vol 26 (20) ◽  
pp. 6214
Author(s):  
Garland Kgosi More ◽  
Stephen Meddows-Taylor ◽  
Gerhard Prinsloo
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Proton Nuclear Magnetic Resonance ◽  
Antioxidant Compounds ◽  
Scavenging Activity ◽  
Frap Assay ◽  
Modeling Tools ◽  
Antioxidant Power

The genus Vachellia, previously known as Acacia, belongs to the family Fabaceae, subfamily Leguminosae, which are flowering plants, commonly known as thorn trees. They are traditionally used medicinally in various countries including South Africa for the treatment of ailments such as fever, sore throat, Tuberculosis, convulsions and as sedatives. The aim of this study was to determine biochemical variations in five Vachellia species and correlate their metabolite profiles to antioxidant activity using a chemometric approach. The antioxidant activity of five Vachellia aqueous-methanolic extracts were analyzed using three methods: 2,2-di-phenyl-1-picrylhydrazyl (DPPH) radical scavenging assay, 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS+) analysis and the ferric reducing antioxidant power (FRAP) assay by means of serial dilution and bioautography with the thin-layer chromatography (TLC) method. Amongst the Vachellia extracts tested, V. karroo, V. kosiensis and V. xanthophloea demonstrated the highest DPPH, ABTS+ and FRAP inhibitory activity. The antioxidant activities of DPPH were higher than those obtained by ABTS+, although these values varied among the Vachellia species. Proton nuclear magnetic resonance (1H NMR), coupled with multivariate statistical modeling tools such as principal component analysis (PCA) and orthogonal partial least squares discriminant analysis (OPLS-DA), were performed to profile metabolites responsible for the observed activity. The OPLS-DA categorized the five Vachellia species, separating them into two groups, with V. karroo, V. kosiensis and V. xanthophloea demonstrating significantly higher radical scavenging activity than V. tortilis and V. sieberiana, which clustered together to form another group with lower radical scavenging activity. Annotation of metabolites was carried out using the ultra-high-performance liquid chromatography–quadrupole time-of-flight mass spectrometry (UHPLC-qTOF-MS), and it tentatively identified 23 metabolites of significance, including epigallocatechin (m/z = 305.0659), methyl gallate (m/z = 183.0294) and quercetin (m/z = 301.0358), amongst others. These results elucidated the metabolites that separated the Vachellia species from each other and demonstrated their possible free radical scavenging activities.

scholarly journals The Antibacterial and Antioxidant Activity of Centella Asiatica Chloroform Extract-loaded Gelatin Nanoparticles

2018 ◽  
Vol 187 ◽  
pp. 02002
Author(s):  
Kittiya Kesornbuakao ◽  
Suksun Amornraksa ◽  
Malinee Sriariyanun ◽  
Suvaluk Asavasanti ◽  
Patchanee Yasurin
Keyword(s):  
Antioxidant Activity ◽  
Radical Scavenging ◽  
Chloroform Extract ◽  
Dpph Radical ◽  
Gelatin Nanoparticles ◽  
Drug Bioavailability ◽  
Antioxidant Power ◽  
Significant Difference ◽  
One Step ◽  
Dpph Radical Scavenging

Nanoencapsulation of Centella asaitica (CA) crude chloroform extract seems to be an attractive approach that may improve drug bioavailability and drug delivery system. In the study, CA extract-loaded gelatin nanoparticles (CGNP) were developed by gelatin one-step and two-step desolvation methods, using three different ratios between CA crude chloroform extract and gelatin (1:2, 1:3, and 1:4 w/w). The antibacterial and antioxidants activities of CGNP and CA were compared. The antioxidant activity of CGNP and CA was evaluated by using DPPH radical scavenging assay and ferric reducing antioxidant power (FRAP) assay. Results showed an enhancement of CGNP antibacterial activity against food borne pathogen. The highest inhibition diameters and FRAP determined from CGNP prepared by one-step at 1:4 ratios (1.03±0.39 cm and 1.23±019 mmol Fe2+/mg dried weight, respectively). However, CGNP was no significant difference in DPPH radical scavenging activity compared to CA. These results provide useful information for developing effective nanoencapsulation of CA as effective natural ingredient.

scholarly journals Blackberry (Rubus spp.): influence of ripening and processing on levels of phenolic compounds and antioxidant activity of the 'Brazos' and 'Tupy' varieties grown in Brazil

2015 ◽  
Vol 45 (4) ◽  
pp. 744-749 ◽  
Author(s):  
Acácio Antonio Ferreira Zielinski ◽  
Caroline Goltz ◽  
Maira Akemi Casagrande Yamato ◽  
Suelen Ávila ◽  
Elisa Yoko Hirooka ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Total Phenolic ◽  
Antioxidant Compounds ◽  
Anthocyanin Content ◽  
Antioxidant Power ◽  
Significant Difference ◽  
Ferric Reducing Antioxidant Power ◽  
Fermented Beverage ◽  
Tropical Climates

Fruits from temperate and tropical climates which have high levels of antioxidant compounds are the source of numerous studies concerning the correlation with benefits to human health. The objectives of this study were to quantify the anthocyanins and phenolic compounds and also to measure the antioxidant activity (ferric reducing antioxidant power - FRAP) of blackberries from two varieties grown in southern Brazil ('Brazos' and 'Tupy') at three stages of ripening; unripe, semi-ripe, ripe and their products (pulp and fermented products). During fruit ripening it was observed that weight, size, diameter and sugars increase significantly and acidity decreased significantly. The anthocyanin content ranged from 4.19 (semi-ripe 'Tupy' variety) to 205.75mg 100g-1 (ripe 'Brazos' variety). The highest levels of phenolic compounds were observed for the unripe fruit of both varieties, while antioxidant activity showed no significant difference during the ripening stages. The studied pulp showed a high content of phenolic compounds (ten times higher than that found in the ripe fruits). The anthocyanin content and antioxidant activity did not show the same increase due to the degradation of anthocyanins caused by the heat treatment that was used. The alcoholic fermented beverage made from blackberries remained stable (total phenolic compounds and antioxidant activity) during two years of storage, but the in third year a significant reduction in antioxidant activity was observed. These results can be important for establishing the shelf life of this kind of product made with blackberry

scholarly journals Evaluation of protein content and antioxidant activity of edible bird’s nest by various methods

2020 ◽  
Vol 10 (2) ◽  
pp. 5277-5283
Keyword(s):  
Antioxidant Activity ◽  
Protein Content ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Total Phenolic ◽  
Antioxidant Power ◽  
Extraction Procedures ◽  
Significant Difference ◽  
Edible Bird’S Nest ◽  
Free Radical Scavenging Assay

The aim of this study was to evaluate the protein content and antioxidant activity of instant and raw Edible bird’s nest (EBN). This study was conducted using two types of EBNs, which are instant and raw samples. All EBN samples were extracted via three types of extraction method, namely, salt, alkaline, and hot extractions. Lowry’s method was used to analyze protein content and the antioxidant activities were analyzed via free radical scavenging assay 1,1-diphenyl-2-pycrylhydrazyl (DPPH), total phenolic content (TPC) assay and ferric reducing antioxidant power (FRAP). This study showed that the highest protein was 2165.90 µg/mL obtained from raw EBN extracted via alkaline solution. In addition, it was also found that the protein concentration of raw EBN was higher compared to instant EBN for all types of extraction procedures. Results from antioxidant assay showed that there was no significant difference between DPPH and FRAP of both EBN. Moreover, TPC results showed that the there was no phenolic compounds detected via all extraction procedures.

Assessment of antioxidant and antibacterial activities of crude extracts of verbena officinalis Linn root or Atuch (Amharic)

2019 ◽  
Author(s):  
Chem Int
Keyword(s):  
Antioxidant Activity ◽  
Methanolic Extract ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Dry Weight ◽  
Antimicrobial Activities ◽  
Total Flavonoid ◽  
Root Extract ◽  
Antioxidant Power ◽  
Crude Extracts

Verbena officinalis Linn is a traditionally known medicinal plant which is used against a number of diseases including inflammatory conditions. In this study its antioxidant activity (reducing powers, 2, 2-diphenyl-1-picrylhydrazyl (DPPH) scavenging activities), ferric reduction activity potential (FRAP), total flavonoid concentration and antimicrobial activities of 80%, 90%, 100% methanol and chloroform extracts of V. officinalis Linn root and 90% and100% methanol leaf extracts were determined. Its antioxidant activity increases with increase in amount of extract (10% to 40%v/v). Total flavonoid content (TFC) varied from 73.32±0.002 mgQE/100g of dry weight (90% methanol) to 42.39±0.032 mgQE/100g dry weight (chloroform), 2,2-diphenyl-1-picrylhydrazyl (DPPH), radical scavenging activity (%) was varied between 87.39% (90% methanol) to 45.57% (chloroform) while Ferric reducing antioxidant power was observed between 372.93±0.04 mgAAE/100 g extract (90% methanol) to 129.41±0.026 mgAAE/100 g chloroform in the root extract. The methanolic extract of the leaf showed less antioxidant activity than the methanolic extract of the root. Crude extracts of V. officinalis root showed various degree of antimicrobial activity towards drug resistance microbial pathogens. Growth inhibition tests against bacterial pathogens demonstrated concentration dependence. Moreover, gram positive bacteria were more susceptible to V. officinalis root extract when compared to gram negative bacteria. In general V. officinalis root and leave extracts possess strong antioxidant and antimicrobial activities.

Synthesis and antioxidant activity of new selenium-containing quinolines

2020 ◽  
Vol 16 ◽  
Author(s):  
Benedetta Bocchini ◽  
Bruna Goldani ◽  
Fernanda S.S. Sousa ◽  
Paloma T. Birmann ◽  
Cesar A. Brüning ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Superoxide Dismutase ◽  
Radical Scavenging ◽  
Building Blocks ◽  
Antioxidant Potential ◽  
In Vitro Assays ◽  
Pharmacological Activities ◽  
Antioxidant Power ◽  
Antioxidant Agents

Background: Quinoline derivatives have been attracted much attention in drug discovery and synthetic derivatives of these scaffolds present a range of pharmacological activities. Therefore, organoselenium compounds are valuable scaffolds in organic synthesis because their pharmacological activities and their use as versatile building blocks for regio-, chemio-and stereoselective reactions. Thus, the synthesis of selenium-containing quinolines has great significance, and their applicability range from simple antioxidant agents, to selective DNA-binding and photocleaving agents. Objective: In the present study we describe the synthesis and antioxidant activity in vitro of new 7-chloroN(arylselanyl)quinolin-4-amines 5 by the reaction of 4,7-dichloroquinoline 4 with (arylselanyl)-amines 3. Methods: For the synthesis of 7-chloro-N(arylselanyl)quinolin-4-amines 5, we performed the reaction of (arylselanyl)- amines 3 with 4,7-dichloroquinoline 4 in the presence of Et3N at 120 °C in a sealed tube. The antioxidant activities of the compounds 5 were evaluated by the following in vitro assays: 2,2- diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity, 2,2-azinobis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS), ferric ion reducing antioxidant power (FRAP), nitric oxide (NO) scavenging and superoxide dismutase-like activity (SOD-Like). Results: 7-Chloro-N(arylselanyl)quinolin-4-amines 5a-d has been synthesized in yields ranging from 68% to 82% by the reaction of 4,7-dichloroquinoline 4 with arylselanyl-amines 3a-d using Et3N as base, at 120 °C, in a sealed tube for 24 hours and tolerates different substituents, such as -OMe and -Cl, in the arylselanyl moiety. The obtained compounds 5a-d presented significant results with respect to the antioxidant potential, which had effect in the tests of inhibition of radical’s DPPH, ABTS+ and NO, as well as in the test that evaluates the capacity (FRAP) and in the superoxide dismutase-like activity assay (SOD-Like). It is worth mentioning that 7-chloro-N(arylselanyl)quinolin-4-amine 5b presented excellent results, demonstrating a better antioxidant capacity when compared to the others. Conclusion: According to the obtained results 7-chloro-N(arylselanyl)quinolin-4-amines 5 were synthesized in good yields by the reaction of 4,7-dichloroquinoline with arylselanyl-amines and tolerates different substituents in the arylselanyl moiety. The tested compounds presented significant antioxidant potential in the tests of inhibition of DPPH, ABTS+ and NO radicals, as well as in the FRAP and superoxide dismutase-like activity assays (SOD-Like).

scholarly journals Inhibitory Effect of Antidesma bunius Fruit Extract on Carbohydrate Digestive Enzymes Activity and Protein Glycation In Vitro

Antioxidants ◽  
2020 ◽  
Vol 10 (1) ◽  
pp. 32
Author(s):  
Pattamaporn Aksornchu ◽  
Netima Chamnansilpa ◽  
Sirichai Adisakwattana ◽  
Thavaree Thilavech ◽  
Charoonsri Choosak ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Radical Scavenging Activity ◽  
Digestive Enzyme ◽  
Radical Scavenging ◽  
Protein Glycation ◽  
Trolox Equivalent Antioxidant Capacity ◽  
Fruit Extract ◽  
Antioxidant Power ◽  
Ic50 Values

Antidesma bunius (L.) spreng (Mamao) is widely distributed in Northeastern Thailand. Antidesma bunius has been reported to contain anthocyanins, which possess antioxidant and antihypertensive actions. However, the antidiabetic and antiglycation activity of Antidesma bunius fruit extract has not yet been reported. In this study, we investigated the inhibitory activity of anthocyanin-enriched fraction of Antidesma bunius fruit extract (ABE) against pancreatic α-amylase, intestinal α-glucosidase (maltase and sucrase), protein glycation, as well as antioxidant activity. A liquid chromatography-tandem mass spectrometry (LC-MS/MS) chromatogram revealed that ABE contained phytochemical compounds such as cyanidin-3-glucoside, delphinidin-3-glucoside, ellagic acid, and myricetin-3-galactoside. ABE inhibited intestinal maltase and sucrase activity with the IC50 values of 0.76 ± 0.02 mg/mL and 1.33 ± 0.03 mg/mL, respectively. Furthermore, ABE (0.25 mg/mL) reduced the formation of fluorescent AGEs and the level of Nε-carboxymethyllysine (Nε-CML) in fructose and glucose-induced protein glycation during four weeks of incubation. During the glycation process, the protein carbonyl and β-amyloid cross structure were decreased by ABE (0.25 mg/mL). In addition, ABE exhibited antioxidant activity through DPPH radical scavenging activity and Trolox equivalent antioxidant capacity (TEAC) with the IC50 values 15.84 ± 0.06 µg/mL and 166.1 ± 2.40 µg/mL, respectively. Meanwhile, ferric reducing antioxidant power (FRAP) showed an EC50 value of 182.22 ± 0.64 µg/mL. The findings suggest that ABE may be a promising agent for inhibiting carbohydrate digestive enzyme activity, reducing monosaccharide-induced protein glycation, and antioxidant activity.

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