scholarly journals Purshia plicata Triggers and Regulates Proteins Related to Apoptosis in HeLa Cancer Cells

Plants ◽  
2021 ◽  
Vol 10 (12) ◽  
pp. 2559
Author(s):  
Patricia Álvarez-Ortiz ◽  
Juan Ascacio-Valdés ◽  
Ileana Vera-Reyes ◽  
Cecilia Esparza-González ◽  
Raúl Rodríguez-Herrera ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Cancer Cells ◽  
Cell Lines ◽  
High Performance ◽  
Public Health Problem ◽  
Bioactive Compound ◽  
Liquid Chromatography Mass Spectrometry ◽  
Cost Of Treatment ◽  
Traditional Therapies ◽  
Mass Spectrometry Mass Spectrometry

Cervical cancer represents a public health problem, develops resistance to traditional therapies and cost-of-treatment is high. These disadvantages have led to the search for alternative bioactive-compound-based therapies. Said bioactive compounds include phenolic compounds, flavonoids, and tannins. The present study aimed to evaluate the therapeutic effect of a P. plicata extract on the HeLa cell line. Viability and apoptosis assays were run on the two cell lines treated with the extract. The peptides, up- and down-expressed in both cell lines, were identified by PDQuest analysis software and high-performance liquid chromatography/mass spectrometry/mass spectrometry (HPLC/MS/MS). Our results show that a 500 mg/L treatment deregulated cell viability, with different apoptotic morphologies observed which are associated with the presence of bio-compounds, which up- and down-regulated the peptides. In conclusion, P. plicata regulates proteins associated with apoptosis in HeLa cancer cells.

scholarly journals QUANTITATIVE DETERMINATION OF AMLODIPINE FROM CACO-2 CELL MONOLAYERS BY HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY–MASS SPECTROMETRY/MASS SPECTROMETRY

2018 ◽  
Vol 11 (6) ◽  
pp. 204 ◽  
Author(s):  
Liliya Logoyda
Keyword(s):  
Mass Spectrometry ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Formic Acid ◽  
High Performance ◽  
Liquid Chromatography Mass Spectrometry ◽  
Cell Monolayers ◽  
Chromatography Mass Spectrometry ◽  
Mass Spectrometry Mass Spectrometry

Objective: A simple, rapid high-performance liquid chromatography–mass spectrometry/mass spectrometry (MS/MS) method was developed for the determination of amlodipine from confluent Caco-2 monolayers and from aqueous solution.Methods: Chromatography was achieved on Discovery C18, 50×2.1 mm, 5 μm column. Samples were chromatographed in a gradient mode (eluent A [acetonitrile-water-formic acid, 5:95:0.1 v/v], eluent B [acetonitrile-formic acid, 100:0.1 v/v]). The initial content of the eluent B is 0%, which increases linearly by 1.0 min to 100% and to 1.01 min returns to the initial 0%. The mobile phase was delivered at a flow rate of 0.400 ml/min into the MS electrospray ionization chamber. The sample volume was 5 μl.Results: Under these conditions, amlodipine was eluted at 1.61 min. According to the Caco-2 test results, amlodipine appeared to have moderate to high permeability. Recovery value for amlodipine is 52.33%. Caco-2 permeability values for amlodipine are in agreement with BCS Class I classification for these drugs and their known high bioavailability in humans.Conclusion: A new rapid method was developed for the determination of amlodipine from confluent Caco-2 monolayers and from aqueous solution. Acquired results demonstrate that proposed strategy can be effortlessly and advantageously applied for the examination of amlodipine from Caco-2 cell monolayers.

scholarly journals Studies on the formation and synthetic mechanism of related substance G in potassium clavulanate production

2015 ◽  
Vol 51 (1) ◽  
pp. 77-83 ◽  
Author(s):  
Xiaoqing Jin ◽  
Guangxiang Cao ◽  
Xiaoxiao Zhang ◽  
Yuguo Chen ◽  
Liang Wang ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
High Performance Liquid Chromatography ◽  
High Performance ◽  
Extracellular Enzyme ◽  
Liquid Chromatography Mass Spectrometry ◽  
Related Substance ◽  
Fermentation Time ◽  
Potassium Clavulanate ◽  
Mass Spectrometry Mass Spectrometry

The objective of this study was to investigate the formation and synthetic mechanism of related substance G in potassium clavulanate production. The impurity in the potassium clavulanate final product, with a retention time of 13.5 min, was confirmed as related substance G by high performance liquid chromatography-mass spectrometry/mass spectrometry. Related substance G analysis during the production of clavulanic acid showed that this impurity could be synthesized during fermentation, and the amount increased with the fermentation time. Studies on its synthetic mechanism showed that L-tyrosine and succinic acid were the precursors for biosynthesis of related substance G in vivo. The reaction was deduced to be catalyzed by an enzyme. The enzyme was a type of extracellular enzyme present in the fermentation supernatant.

scholarly journals Exploration of production of C14 and C15 bacillomycin D homologues with enzymatic hydrolysis from maize straws using fed-batch fermentation by Bacillus subtilis NS-174

RSC Advances ◽  
2020 ◽  
Vol 10 (11) ◽  
pp. 6725-6734
Author(s):  
Shiquan Qian ◽  
Xuejin Li ◽  
Lu Sun ◽  
Yuanyuan Shen ◽  
Qingyi Ren ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Bacillus Subtilis ◽  
Batch Fermentation ◽  
High Performance ◽  
Antifungal Compounds ◽  
Liquid Chromatography Mass Spectrometry ◽  
Fed Batch ◽  
Fed Batch Fermentation ◽  
Mass Spectrometry Mass Spectrometry ◽  
Bacillomycin D

A strain with strong antifungal activity, Bacillus subtilis NS-174, was identified and the antifungal compounds were purified and structurally analyzed by high performance liquid chromatography-mass spectrometry/mass spectrometry (HPLC-MS/MS).

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