scholarly journals The Omics Hunt for Novel Molecular Markers of Resistance to Phytophthora infestans

Plants ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 61
Author(s):  
Hana Dufková ◽  
Miroslav Berka ◽  
Marie Greplová ◽  
Šarlota Shejbalová ◽  
Romana Hampejsová ◽  
...  
Keyword(s):  
Phytophthora Infestans ◽  
Molecular Mechanisms ◽  
Protein Markers ◽  
Resistance Markers ◽  
Late Blight Disease ◽  
Total Pool ◽  
Resistance Protein ◽  
Blight Disease ◽  
Moderately Resistant ◽  
Resistant Genotypes

Wild Solanum accessions are a treasured source of resistance against pathogens, including oomycete Phytophthora infestans, causing late blight disease. Here, Solanum pinnatisectum, Solanum tuberosum, and the somatic hybrid between these two lines were analyzed, representing resistant, susceptible, and moderately resistant genotypes, respectively. Proteome and metabolome analyses showed that the infection had the highest impact on leaves of the resistant plant and indicated, among others, an extensive remodeling of the leaf lipidome. The lipidome profiling confirmed an accumulation of glycerolipids, a depletion in the total pool of glycerophospholipids, and showed considerable differences between the lipidome composition of resistant and susceptible genotypes. The analysis of putative resistance markers pinpointed more than 100 molecules that positively correlated with resistance including phenolics and cysteamine, a compound with known antimicrobial activity. Putative resistance protein markers were targeted in an additional 12 genotypes with contrasting resistance to P. infestans. At least 27 proteins showed a negative correlation with the susceptibility including HSP70-2, endochitinase B, WPP domain-containing protein, and cyclase 3. In summary, these findings provide insights into molecular mechanisms of resistance against P. infestans and present novel targets for selective breeding.

scholarly journals Response of Local Potato Cultivars to Late Blight Disease (Phytophthora infestans (Mont.) de Bary) under Field Conditions

2020 ◽  
Vol 3 (1) ◽  
pp. 28-37
Author(s):  
Rishav Pandit ◽  
Ravi Bhatta ◽  
Pooja Bhusal ◽  
Basistha Acharya ◽  
Subash Subedi ◽  
...  
Keyword(s):  
Late Blight ◽  
Phytophthora Infestans ◽  
Disease Incidence ◽  
Block Design ◽  
Potato Cultivar ◽  
Late Blight Resistance ◽  
Potato Cultivars ◽  
Late Blight Disease ◽  
Blight Disease ◽  
Moderately Resistant

Developing host resistance is an economic and long-term approach to disease management; however, resistance reactions that differ depending on the genotypes. Potato late blight is the devastating disease caused due to Phytophthora infestans (Mont.) de Bary.  In order to identify late blight resistance in potato genotypes, seven local potato cultivars (Bardiya Rato Local, Bardiya Seto Local, Cardinal, Deukhuri Rato Local, Deukhuri Seto Local, Kailali Local and Khumal Ujjowal) were evaluated in randomized complete block design (RCBD) with three replications during October 2018 to January 2019 on naturally infested soils in Deukhuri, Dang, Nepal. Results showed that significantly the highest disease incidence (99.17%), and the highest disease severity (88%) were found on Cardinal followed by Deukhuri Rato Local (64%), and Deukhuri Seto Local (60%). Potato cultivar namely Khumal Ujjowal was moderately resistant, whereas Deukhuri Rato local and Deukhuri Seto Local were susceptible to late blight disease. Significantly the highest yield (12.67 t ha-1) was produced by Khumal Ujjowal followed by Bardiya Rato Local (10.78 t ha-1) and Bardiya Seto Local (9.40 t ha-1). The disease incidence and Area under disease progressive curve (AUDPC) value was negatively co-related with the tuber yield. The potato cultivar Khumal Ujjowal followed by Bardiya Rato Local were found moderately resistant to late blight disease in Deukhuri conditions. This study suggests that potato cultivar Bardiya Rato Local can be grown for higher tuber production in Dang and similar topographic regions.

scholarly journals Response of Local Potato Cultivars to Late Blight Disease (Phytophthora infestans (Mont.) de Bary) under Field Conditions

2020 ◽  
Vol 3 (1) ◽  
pp. 28-37
Author(s):  
Rishav Pandit ◽  
Ravi Bhatta ◽  
Pooja Bhusal ◽  
Basistha Acharya ◽  
Subash Subedi ◽  
...  
Keyword(s):  
Late Blight ◽  
Phytophthora Infestans ◽  
Disease Incidence ◽  
Block Design ◽  
Potato Cultivar ◽  
Late Blight Resistance ◽  
Potato Cultivars ◽  
Late Blight Disease ◽  
Blight Disease ◽  
Moderately Resistant

Developing host resistance is an economic and long-term approach to disease management; however, resistance reactions that differ depending on the genotypes. Potato late blight is the devastating disease caused due to Phytophthora infestans (Mont.) de Bary.  In order to identify late blight resistance in potato genotypes, seven local potato cultivars (Bardiya Rato Local, Bardiya Seto Local, Cardinal, Deukhuri Rato Local, Deukhuri Seto Local, Kailali Local and Khumal Ujjowal) were evaluated in randomized complete block design (RCBD) with three replications during October 2018 to January 2019 on naturally infested soils in Deukhuri, Dang, Nepal. Results showed that significantly the highest disease incidence (99.17%), and the highest disease severity (88%) were found on Cardinal followed by Deukhuri Rato Local (64%), and Deukhuri Seto Local (60%). Potato cultivar namely Khumal Ujjowal was moderately resistant, whereas Deukhuri Rato local and Deukhuri Seto Local were susceptible to late blight disease. Significantly the highest yield (12.67 t ha-1) was produced by Khumal Ujjowal followed by Bardiya Rato Local (10.78 t ha-1) and Bardiya Seto Local (9.40 t ha-1). The disease incidence and Area under disease progressive curve (AUDPC) value was negatively co-related with the tuber yield. The potato cultivar Khumal Ujjowal followed by Bardiya Rato Local were found moderately resistant to late blight disease in Deukhuri conditions. This study suggests that potato cultivar Bardiya Rato Local can be grown for higher tuber production in Dang and similar topographic regions.

Evaluation of bio-agents and neem based products against late blight disease (Phytophthora infestans) of potato

2021 ◽  
Vol 74 (1) ◽  
pp. 181-187
Author(s):  
Mehi Lal ◽  
Sorabh Chaudhary ◽  
Sanjay Rawal ◽  
Sanjeev Sharma ◽  
Manoj Kumar ◽  
...  
Keyword(s):  
Late Blight ◽  
Phytophthora Infestans ◽  
Late Blight Disease ◽  
Blight Disease

scholarly journals Phenotypic Characterization of Recent Clonal Lineages of Phytophthora infestans in the United States

Plant Disease ◽  
2013 ◽  
Vol 97 (7) ◽  
pp. 873-881 ◽  
Author(s):  
G. Danies ◽  
I. M. Small ◽  
K. Myers ◽  
R. Childers ◽  
W. E. Fry
Keyword(s):  
United States ◽  
Late Blight ◽  
Phytophthora Infestans ◽  
The United States ◽  
Phenotypic Characterization ◽  
Phenotypic Traits ◽  
Mating Types ◽  
Late Blight Disease ◽  
Blight Disease

Phytophthora infestans, the causal agent of late blight disease, has been reported in the United States and Canada since the mid-nineteenth century. Due to the lack of or very limited sexual reproduction, the populations of P. infestans in the United States are primarily reproducing asexually and, thus, show a simple genetic structure. The emergence of new clonal lineages of P. infestans (US-22, US-23, and US-24) responsible for the late blight epidemics in the northeastern region of the United States in the summers of 2009 and 2010 stimulated an investigation into phenotypic traits associated with these genotypes. Mating type, differences in sensitivity to mefenoxam, differences in pathogenicity on potato and tomato, and differences in rate of germination were studied for clonal lineages US-8, US-22, US-23, and US-24. Both A1 and A2 mating types were detected. Lineages US-22, US-23, and US-24 were generally sensitive to mefenoxam while US-8 was resistant. US-8 and US-24 were primarily pathogenic on potato while US-22 and US-23 were pathogenic on both potato and tomato. Indirect germination was favored at lower temperatures (5 and 10°C) whereas direct germination, though uncommon, was favored at higher temperatures (20 and 25°C). Sporangia of US-24 released zoospores more rapidly than did sporangia of US-22 and US-23. The association of characteristic phenotypic traits with genotype enables the prediction of phenotypic traits from rapid genotypic analyses for improved disease management.

Field application of Bacillus subtilis isolates for controlling late blight disease of potato caused by Phytophthora infestans

2019 ◽  
Vol 22 ◽  
pp. 101366 ◽  
Author(s):  
Bhimanagoud Kumbar ◽  
Riaz Mahmood ◽  
S.N. Nagesha ◽  
M.S. Nagaraja ◽  
D.G. Prashant ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Late Blight ◽  
Phytophthora Infestans ◽  
Field Application ◽  
Late Blight Disease ◽  
Blight Disease

scholarly journals Protection of Susceptible Potato Cultivars Against Late Blight in Mixtures Increases with Decreasing Disease Pressure

2006 ◽  
Vol 96 (7) ◽  
pp. 777-783 ◽  
Author(s):  
F. Pilet ◽  
G. Chacón ◽  
G. A. Forbes ◽  
D. Andrivon
Keyword(s):  
Late Blight ◽  
Potato Cultivar ◽  
Progress Curve ◽  
Late Blight Disease ◽  
Natural Pressure ◽  
Blight Disease ◽  
Disease Pressure ◽  
Moderately Resistant ◽  
The Impact ◽  
Cultivar Mixtures

Cultivar mixtures can reduce potato late blight severity on susceptible cultivars. While alternating rows of susceptible and resistant cultivars would be more acceptable than random mixtures for commercial use, they increase the genotype unit area, which is an unfavorable factor for mixture efficiency, and have been minimally efficient when disease pressure is high. The effects of disease pressure on the performance of alternating rows of cultivars possessing various types and levels of resistance were investigated in 2000 and 2001 near Quito, Ecuador, where natural pressure of late blight is high. The experiments included the highly susceptible cvs. Cecilia in 2000 and LBr37 in 2001, as well as C114 (moderately resistant) and PAN (highly resistant), planted as pure stands and as the three possible two-way combinations. Different disease pressures were obtained with three spraying schedules of a contact fungicide: nontreated, one spray every second week, and one spray weekly. The area under the disease progress curve (AUDPC) on the susceptible cultivar was 0 to 20% less in mixed than in pure plots when no fungicide was applied, 13 to 26% less with a biweekly application of fungicide, and 32 to 53% less with a weekly application. These values are comparable to those obtained in previous experiments in smaller plots with designs maximizing the distance between susceptible plants. No significant differences in mixture performance were observed according to the resistant cultivar included. Effects on yield were minimal, because of the impact of factors other than late blight. Disease pressure therefore appears as a major factor conditioning the efficiency of potato cultivar mixtures against late blight.

Identification and Screening of Introduced Tomato Varieties Resistant to late Blight

2012 ◽  
Vol 610-613 ◽  
pp. 3472-3477
Author(s):  
Dan Luo ◽  
Xi Chun Zhang ◽  
Xiao Han Wen
Keyword(s):  
Late Blight ◽  
Phytophthora Infestans ◽  
New Disease ◽  
Tomato Production ◽  
Disease Resistant ◽  
Resistant Varieties ◽  
Detached Leaves ◽  
Late Blight Disease ◽  
Blight Disease ◽  
Selection Of

Tomato late blight disease that infected by Phytophthora infestans (Mont.) de Bary has already become one of the main obstacles that influence the tomato production. To resolve tomato late blight disease, cultivating the disease-resistant breed is importance. Based on the previous study, different introduced varieties were used as materials in this test for the identification and varieties selection of tomato resistant to late blight, by measuring the DIS of inoculated seedlings and detached leaves. It indicated that Jingle 502 and O-33-1 are possible to become resistant varieties to late blight or the parents for cultivating new disease resistant varieties.

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