scholarly journals RUST: A Robust, User-Friendly Script Tool for Rapid Measurement of Rust Disease on Cereal Leaves

Plants ◽  
2020 ◽  
Vol 9 (9) ◽  
pp. 1182
Author(s):  
Luis M. Gallego-Sánchez ◽  
Francisco J. Canales ◽  
Gracia Montilla-Bascón ◽  
Elena Prats
Keyword(s):  
Leaf Rust ◽  
Disease Assessment ◽  
Infection Frequency ◽  
Rust Disease ◽  
Sources Of Resistance ◽  
High Throughput Analysis ◽  
Automated Evaluation ◽  
Rust Diseases ◽  
Color Transformation ◽  
User Friendly

Recently, phenotyping has become one of the main bottlenecks in plant breeding and fundamental plant science. This is particularly true for plant disease assessment, which has to deal with time-consuming evaluations and the subjectivity of visual assessments. In this work, we have developed an open source Robust, User-friendy Script Tool (RUST) for semi-automated evaluation of leaf rust diseases. RUST runs under the free Fiji imaging software (developed from ImageJ), which is a well-recognized software among the scientific community. The script enables the evaluation of leaf rust diseases using a color transformation tool and provides three different automation modes. The script opens images sequentially and records infection frequency (pustules per area) (semi-)automatically for high-throughput analysis. Furthermore, it can manage several scanned leaf segments in the same image, consecutively selecting the desired segments. The script has been validated with nearly 900 samples from 80 oat genotypes ranging from resistant to susceptible and from very light to heavily infected leaves showing a high accuracy with a Lin’s concordance correlation coefficient of 0.99. The analysis show a high repeatability as indicated by the low variation coefficients obtained when repeating the measurement of the same samples. The script also has optional steps for calibration and training to ensure accuracy, even in low-resolution images. This script can evaluate efficiently hundreds of leaves facilitating the screening of novel sources of resistance to this important cereal disease.

scholarly journals Wheat sRNA transcriptome reveals R-gene associated distinct temporal miRNA profiles in response to stem rust and leaf rust diseases

2021 ◽  
Author(s):  
Minu M Nair ◽  
Hari Krishna S Kumar ◽  
Jyothsna S ◽  
Krishna TS ◽  
Manjunatha C ◽  
...  
Keyword(s):  
Disease Management ◽  
Leaf Rust ◽  
Stress Responses ◽  
Stem Rust ◽  
Defense Mechanisms ◽  
Regulatory Elements ◽  
Small Rna Sequencing ◽  
Rust Disease ◽  
Rust Diseases ◽  
Passenger Strand

Abstract Wheat rusts, the dreadful fungal diseases caused by members of the Puccinia genus remain as a major threat to global wheat production and food security. The R-gene mediated resistance has been employed as an efficient approach to develop rust resistant varieties. However, evolution of new fungal races and infection strategies put forward the urgency of unravelling novel genomics assisted molecular players, including non-coding RNAs for rust disease management. MicroRNAs are crucial regulators of genes associated with plant growth, development, abiotic and biotic stress responses. In this study, high throughput small RNA sequencing was performed on susceptible and resistant wheat near-isogenic lines inoculated with stem rust and leaf rust, together with mock inoculated controls. While miRNA mining in stem rust inoculated libraries revealed a total of twenty-six known and seven novel miRNAs, the search in leaf rust libraries culminated with twenty-two known and four novel miRNAs. The identified candidate miRNAs constituted both guide and passenger miRNA strands along with their isomiRs. Temporal expression pattern of differentially expressed miRNAs pinpoints their possible role during the progress of infection. Comparative analysis provides a better understanding of altered miRNA profiles associated with Sr36 and Lr45 disease resistance genes in response to stem rust and leaf rust, respectively. Also, the disclosure of guide strand, passenger strand and a variant of novel-Tae-miR02 with various sub-genomal origins might serve as an important common link between stem rust and leaf rust defense mechanisms downstream to the respective R-genes. Further, cis-regulatory elements that modulate MIR genes, targets of identified miRNAs related to various signal transduction pathways, and miR-SSRs which might be useful for rust disease control, are the emphasized attributes of our results. The outcome from this simultaneous study of miRNA profiles among two rust diseases and further characterization of the identified miRNAs can contribute to the expanding rust disease management programs.

scholarly journals High-Throughput Analysis of Selected Urinary Hydroxy Polycyclic Aromatic Hydrocarbons by an Innovative Automated Solid-Phase Microextraction

Molecules ◽  
2018 ◽  
Vol 23 (8) ◽  
pp. 1869 ◽  
Author(s):  
Stefano Dugheri ◽  
Alessandro Bonari ◽  
Matteo Gentili ◽  
Giovanni Cappelli ◽  
Ilenia Pompilio ◽  
...  
Keyword(s):  
Polycyclic Aromatic Hydrocarbons ◽  
High Throughput ◽  
Aromatic Hydrocarbons ◽  
High Throughput Screening ◽  
Solid Phase Microextraction ◽  
Solid Phase ◽  
User Friendliness ◽  
High Throughput Analysis ◽  
Polycyclic Aromatic ◽  
User Friendly

High-throughput screening of samples is the strategy of choice to detect occupational exposure biomarkers, yet it requires a user-friendly apparatus that gives relatively prompt results while ensuring high degrees of selectivity, precision, accuracy and automation, particularly in the preparation process. Miniaturization has attracted much attention in analytical chemistry and has driven solvent and sample savings as easier automation, the latter thanks to the introduction on the market of the three axis autosampler. In light of the above, this contribution describes a novel user-friendly solid-phase microextraction (SPME) off- and on-line platform coupled with gas chromatography and triple quadrupole-mass spectrometry to determine urinary metabolites of polycyclic aromatic hydrocarbons 1- and 2-hydroxy-naphthalene, 9-hydroxy-phenanthrene, 1-hydroxy-pyrene, 3- and 9-hydroxy-benzoantracene, and 3-hydroxy-benzo[a]pyrene. In this new procedure, chromatography’s sensitivity is combined with the user-friendliness of N-tert-butyldimethylsilyl-N-methyltrifluoroacetamide on-fiber SPME derivatization using direct immersion sampling; moreover, specific isotope-labelled internal standards provide quantitative accuracy. The detection limits for the seven OH-PAHs ranged from 0.25 to 4.52 ng/L. Intra-(from 2.5 to 3.0%) and inter-session (from 2.4 to 3.9%) repeatability was also evaluated. This method serves to identify suitable risk-control strategies for occupational hygiene conservation programs.

Resistance of Tangmai 4 wheat to powdery mildew, stem rust, leaf rust, and stripe rust and its chromosome composition

2004 ◽  
Vol 84 (4) ◽  
pp. 1015-1023 ◽  
Author(s):  
H. J. Li ◽  
R. L. Conner ◽  
B. D. McCallum ◽  
X. M. Chen ◽  
H. Su ◽  
...  
Keyword(s):  
Powdery Mildew ◽  
Leaf Rust ◽  
Stripe Rust ◽  
Stem Rust ◽  
Northern China ◽  
Western Canada ◽  
C Banding ◽  
Rust Diseases ◽  
Hard Red Winter Wheat ◽  
Secale Cereale L

The hard red winter wheat Tangmai 4 did not develop symptoms of infection following inoculation with powdery mildew (Erysiphe graminis DC. f. sp. tritici E. Marchal) isolates from regions of western Canada and northern China. Tangmai 4 exhibited resistance to stem rust (Puccinia graminis Pers. f. sp. tritici Eriks. & Henn.) and leaf rust (P. triticina Eriks.) races from western Canada. This wheat line was resistant to individual stripe rust (P. striiformis Westend. f. sp. tritici Eriks.) races from the U.S. and Canada. Sequential C-banding and genomic in situ hybridization (GISH), and electrophoretic analyses of high molecular weight glutenins and gliadins demonstrated that Tangmai 4 carried a pair of T1BL·1RS wheat-rye (Secale cereale L.) translocated chromosomes. Since the genes located on T1BL·1RS are no longer effective in controlling powdery mildew and the rust diseases, Tangmai 4 must carry additional genes for resistance to these diseases, which makes it a valuable resource for the improvement of resistance in wheat against these diseases. Key words: T1BL·1RS translocation, disease resistance, sequential C-banding and GISH, glutenin, gliadin

scholarly journals Sources of resistance to fungal diseases for selection of spring soft wheat

2020 ◽  
pp. 45-49
Author(s):  
Tatyana Yuryevna Taranova ◽  
Alexander Ivanovich Kincharov ◽  
Elena Anatolyevna Demina ◽  
Olga Sergeevna Mullayanova
Keyword(s):  
Powdery Mildew ◽  
Leaf Rust ◽  
Climatic Conditions ◽  
Fungal Diseases ◽  
Soft Wheat ◽  
Sources Of Resistance ◽  
Middle Volga Region ◽  
New Genes ◽  
Spring Soft Wheat ◽  
Resistance To Fungal Diseases

For breeding to create varieties resistant to fungal diseases, it is effective to work in the search for new genes of resistance to pathogens, as well as to constantly update the gene pool of spring wheat. The researches were conducted in 2014-2019 in the Samara region. The object of study was 352 collection samples of spring soft wheat of various ecological and geographical groups. The assessment of samples for resistance to fungal pathogens was carried out against a natural infectious background, including in years with a strong development of certain diseases (powdery mildew – 2014, brown rust – 2016, 2017). Accounting of plant damage by fungal diseases was carried out according to generally accepted phytopathological methods. Since 2000, the laboratory has studied and developed more than one thousand collection samples, formed working characteristic collections of samples for resistance to leaf rust (142), powdery mildew (185) and with complex resistance to these diseases (100). According to the results of six-year studies, collection samples with complex resistance to leaf rust and powdery mildew were identified: Kinelskaya yubileynaya, Erythrospermum 4112, Erythrospermum 4143, Erythrospermum 4144, Erythrospermum 4146, Erythrospermum 4147, Lutescens 6045/7, Erythrospermum 6310/10-63 (Kinel), Khutoryanka (Tambov), Ulyanovskaya 105, Yaritsa (Ulyanovsk), Tulaykovskaya 108 (Bezenchuk), Sigma 2 (Omsk), KVS Aquilon (Germany). Relatively resistant to root rot pathogens samples were noted: Kinelskaya 2010, Lutescens 3960, Erythrospermum 4112, Erythrospermum 4171, Lutescens 4394 (Kinel). The selected varieties and breeding lines are recommended by us as sources of resistance to fungal diseases for use in breeding programs of the middle Volga region and research sites with similar agro-climatic conditions.

scholarly journals INDUCTION OF SYSTEMIC ACQUIRED RESISTANCE OF WHEAT SEEDLINGS AGAINST LEAF RUST DISEASE.

2008 ◽  
Vol 33 (1) ◽  
pp. 243-256
Author(s):  
Marian Thabet ◽  
E. Gado ◽  
M. Najeeb ◽  
S. El -Deeb
Keyword(s):  
Leaf Rust ◽  
Systemic Acquired Resistance ◽  
Acquired Resistance ◽  
Rust Disease ◽  
Wheat Seedlings

scholarly journals Virulence surveillance of wheat black stem rust fungus

2014 ◽  
Vol 11 (2) ◽  
pp. 803-812
Author(s):  
Baghdad Science Journal
Keyword(s):  
Leaf Rust ◽  
Disease Severity ◽  
Stem Rust ◽  
Yellow Rust ◽  
Rust Fungus ◽  
The North ◽  
Rust Diseases ◽  
Northern Regions ◽  
Infection Disease ◽  
Wheat Rust

General survey for wheat rust diseases in Iraqi fields was done during the seasons of 2010, 2011 and 2012. The survey covered different fields in southern, middle and northern regions. Results of the first season indicated that most of Iraqi cultivars such as Tmmoze2, IPA 99 and Mexipak showed different types of susceptibility to both yellow and leaf rust infection. Disease severity increased when the conditions were favorable for infections with using susceptible cultivars. The severity of leaf rust was less in the north region comparing with the middle and south regions. Most of the introduced cultivars such as Sham6 and Cimmyto showed susceptible reaction to yellow and leaf rust. Yellow rust was in epiphytotic form at the Iraqi-Syrian-Turkish triangle where the disease severity was 100%. Low disease severity of stem rust was observed on some cultivars (1-5%), except for the cultivar Mexipak which showed 40%S in Najaf. Rusts at season of 2011 were restricted mostly in Baghdad and the yellow rust was dominant. The AUDPC of 15 wheat cultivars showed that Sawa and Sali were highly susceptible to the three types of rusts while Babil113 and Tamoze2 were resistant. No rusts were detected at season 2012.

scholarly journals Stability of Arabica coffee genotype (Coffea arabica L.) against leaf rust (Hemileia vastatrix)

2021 ◽  
Vol 748 (1) ◽  
pp. 012002
Author(s):  
Sabam Malau ◽  
Albiner Siagian ◽  
Maria Rumondang Sihotang
Keyword(s):  
Leaf Rust ◽  
Block Design ◽  
Research Result ◽  
Dry Season ◽  
Environment Interaction ◽  
Rust Disease ◽  
Hemileia Vastatrix ◽  
Arabica Coffee ◽  
Coffee Leaf Rust ◽  
Rust Severity

Abstract Coffee is now experiencing a serious threat from fungus Hemileia vastatrix which caused epidemic of rust disease in America, Africa, and Asia. As solution, the use of resistant cultivars is the best way. However, interaction between genotype and environment can change the rank of genotypes that shows instability of these genotypes against leaf rust. Purpose of this research was to study stability of genotypes of Arabica coffee against coffee leaf rust. A field experiment was arranged as factorial randomized complete block design with 2 factors (genotypes and climate zones) with three replication. The observed parameters were branch rust incidence, leaf rust incidence, and leaf rust severity. This research result showed significant genotype x environment interaction in all variables. Length of dry season is the most important factor affecting coffee leaf rust because it had the highest correlation coefficient with leaf rust severity (r = 0.662**). Less length of dry season should be the first criteria for selection of coffee farms. The most desired genotype was G7 which performed low leaf rust severity (7.71%) and had a stable resistance indicated by the same leaf rust severity in all environments and 6 SMg. Due to the significant interaction between genotypes and the environment, the genotype to be planted in a region must be tested in that region first.

Development of new bread wheat resistant mutants for Ug99 rust disease (Puccinia graminis f. sp. tritici).

2021 ◽  
pp. 312-319
Author(s):  
Abdulwahid Saif ◽  
Aref Al-Shamiri ◽  
Abdulnour Shaher
Keyword(s):  
Bread Wheat ◽  
Adult Stage ◽  
Yellow Rust ◽  
Seedling Stage ◽  
Yield Potential ◽  
Growth Stages ◽  
Rust Disease ◽  
Wheat Varieties ◽  
Rust Diseases ◽  
Mutant Lines

Abstract M3 derived mutants from two bread wheat varieties, namely, 'Giza 186' and 'Saha 93', were screened for resistance to the rust Ug99 at two locations in Njoro (Kenya) and in Tihama (Yemen). At Tihama, two mutants of 'Giza 186' (G-M2-2010-1-28 and G-M2-2010-41-52) and four mutants of 'Saha 93' (S-M2-2010-16-12, S-M2-2010-21-13, S-M2-2010-22-14 and S-M2-2010-27-15) were seen to be resistant at both seedling and adult stages while their parents were resistant at seedling stage and susceptible at adult stage. In Kenya, the resistance score of the mutants was slightly different from those obtained at Tihama. The mutants G-M2-2010-1-28 and G-M2-2010-41-52 were stable in their level of resistance recorded at Tihama, but only two mutants of 'Saha 93' (S-M2-2010-16-12 and S-M2-2010-27-15) were resistant at both growth stages. S-M2-2010-22-14 and S-M2-2010-21-13 were resistant at the seedling stage while susceptible at adult stage. Further selection on these mutants for yield potential, agronomic performance and yellow rust disease resistance, as well as on selected mutants of both 'Giza 186' and 'Saha 93', at M5-M6 stages identified superior mutant lines compared with the two parents 'Saha 93' and 'Giza 186'. These included the line Erra-010-GM2w-41-52-40, which ranked first in yield (3768 kg/ha), followed by the lines Erra-010-SwM2-16-12-19, Erra-010-GM2w-1-28-18 and Erra-010-SwM2-22-14-6. Moreover, it can be concluded that Erra-010-GM2w-41-52-40 and Erra-010-SwM2-16-12-19 are highly recommended for their resistance to stem and yellow rust diseases as well as for yield potential and preference by farmers. Therefore, efforts are in progress to increase their seeds for dissemination over a wide range of farmers and wheat areas where rust diseases are an epidemic, and for registration of the lines as improved mutant varieties.

Determination of radiosensitivity of Coffea arabica var. 'Venecia' seeds to gamma-ray irradiation.

2021 ◽  
pp. 320-325
Author(s):  
Reina Céspedes ◽  
Noel Arrieta ◽  
Miguel Barquero ◽  
Ana Abdelnour ◽  
Nielen Stephan ◽  
...  
Keyword(s):  
Seed Germination ◽  
Coffea Arabica ◽  
Gamma Ray ◽  
Raw Materials ◽  
New Technology ◽  
Mutation Breeding ◽  
Mutation Induction ◽  
Rust Disease ◽  
Sources Of Resistance ◽  
Breeding Programmes

Abstract Coffee is one of the most commercially available raw materials, being the tropical product with the highest market value in the world. In Costa Rica it is the third most important product for agricultural exports and provides the main income for many families in the country. However, coffee is under threat due to coffee leaf rust disease (CLR). Mutation breeding in coffee is a promising approach to develop new varieties resistant to CLR. As a new technology for coffee, basic tests related to mutation induction need to be done. The plant material used was Coffea arabica var. 'Venecia' seeds, with a moisture content of 27.3%. The applied irradiation doses were 0, 80, 100, 120, 140, 160 and 180 Gy. For each treatment, three replicates of 200 g were used, with a seed number range of 765-808 units per replicate. The irradiated seeds were planted on the same day. Eighty days after treatment the number of seedlings was quantified, the hypocotyl height and radicle length were measured and the opening of cotyledons was determined for each dose. The effects of the radiation doses on seed germination frequency were recorded. At the dose of 80 Gy, germination was reduced over the control by 9.65%, at 100 Gy by 34.06%, at 120 Gy by 52.76%, at 140 Gy by 60.24%, at 160 Gy by 65.56% and at 180 Gy by 75.40%. Seedling growth was affected and a delay in opening of the cotyledons was observed at higher doses. This radiosensitivity test, based on seed germination as compared with unirradiated control, revealed that the LD50 for the variety tested is in the range 100-120 Gy experimentally, and according to the regression is 125 ± 30 Gy. This dose will be used for further bulk experiments and is of great importance, because the LD50 is considered as the range where the appearance of useful mutations in breeding programmes is favoured. The establishment of these parameters is a necessary advance to continue with measurements of genetic and phenotypical parameters to implement mutation breeding in coffee looking for new sources of resistance against CLR.

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