scholarly journals Comparison of Surface Functionalization of PLGA Composite to Immobilize Extracellular Vesicles

Polymers ◽  
2021 ◽  
Vol 13 (21) ◽  
pp. 3643
Author(s):  
Jiwon Woo ◽  
Kyoung-Won Ko ◽  
Seung-Gyu Cha ◽  
Yun Heo ◽  
Dong Keun Han
Keyword(s):  
Surface Modification ◽  
Extracellular Vesicles ◽  
Inflammatory Responses ◽  
Covalent Bond ◽  
Bioactive Molecules ◽  
Receptor Interaction ◽  
Biodegradable Implants ◽  
Vitro Analysis ◽  
In Vitro Analysis

Endothelialization by materials provides a promising approach for the rapid re-endothelialization of a cardiovascular implantation. Although previous studies have focused on improving endothelialization through the immobilization of bioactive molecules onto the surface of biodegradable implants, comparative studies of effective surface modification have not yet been reported. Here, we conducted a comparative study on the surface modification of poly(lactide-co-glycolide) (PLGA)-based composites to graft mesenchymal stem cell-derived extracellular vesicles (MSC-EVs) using three different materials, fibronectin (FN), polyethylenimine (PEI), and polydopamine (PDA), which have different bond strengths of ligand–receptor interaction, ionic bond, and covalent bond, respectively. Further in vitro analysis exhibited that MSC-EVs released from all modified films sustainably, but the MSC-EVs grafted onto the surface coated with PEI are more effective than other groups in increasing angiogenesis and reducing the inflammatory responses in endothelial cells. Therefore, the overall results demonstrated that PEI is a desirable coating reagent for the immobilization of MSC-EVs on the surface of biodegradable implants.

Neutrophil Activation: Control of Shape Change, Exocytosis, and Respiratory Burst

Physiology ◽  
1992 ◽  
Vol 7 (5) ◽  
pp. 215-219
Author(s):  
M Baggiolini ◽  
P Kernen
Keyword(s):  
Signal Transduction ◽  
Respiratory Burst ◽  
Shape Change ◽  
Receptor Interaction ◽  
Intracellular Signal ◽  
Vitro Analysis ◽  
Different Levels ◽  
Neutrophil Leukocytes ◽  
In Vitro Analysis

Neutrophil leukocytes are defence cells that are recruited from the blood into inflamed tissues by chemotactic agonists. Agonist-receptor interaction elicits motion, granule exocytosis, and the respiratory burst. In vitro analysis shows that these responses are controlled at different levels of the intracellular signal transduction process.

In vitro analysis of inflammatory responses following environmental exposure to pharmaceuticals and inland waters

2009 ◽  
Vol 407 (4) ◽  
pp. 1452-1460 ◽  
Author(s):  
Hazem Khalaf ◽  
Lotta Salste ◽  
Patrik Karlsson ◽  
Per Ivarsson ◽  
Jana Jass ◽  
...  
Keyword(s):  
Environmental Exposure ◽  
Inflammatory Responses ◽  
Inland Waters ◽  
Vitro Analysis ◽  
In Vitro Analysis

scholarly journals Identification of Oleanolic Acid as Allosteric Agonist of Integrin αM by Combination of In Silico Modeling and In Vitro Analysis

2021 ◽  
Vol 12 ◽  
Author(s):  
Lu Jin ◽  
Xiaoyu Han ◽  
Xinlei Zhang ◽  
Zhimin Zhao ◽  
Judith Ulrich ◽  
...  
Keyword(s):  
Oleanolic Acid ◽  
Inflammatory Responses ◽  
Dynamics Simulation ◽  
Open Conformation ◽  
Cellular Target ◽  
In Silico Modeling ◽  
Monocytes And Macrophages ◽  
Vitro Analysis ◽  
In Vitro Analysis

Oleanolic acid is a widely distributed natural product, which possesses promising antitumor, antiviral, antihyperlipidemic, and anti-inflammatory activities. A heterodimeric complex formed by integrin αM (CD11b) and integrin β2 (CD18) is highly expressed on monocytes and macrophages. In the current study, we demonstrate that the I domain of αM (αM-I domain) might present a potential cellular target for oleanolic acid. In vitro data show that oleanolic acid induces clustering of αM on macrophages and reduces their non-directional migration. In accordance with experimental data, molecular docking revealed that oleanolic acid binds to the αM-I domain in its extended-open form, the dominant conformation found in αM clusters. Molecular dynamics simulation revealed that oleanolic acid can increase the flexibility of the α7 helix and promote its movement away from the N-terminus, indicating that oleanolic acid may facilitate the conversion of the αM-I domain from the extended-closed to the extended-open conformation. As demonstrated by metadynamics simulation, oleanolic acid can destabilize the local minimum of the αM-I domain in the open conformation partially through disturbance of the interactions between α1 and α7 helices. In summary, we demonstrate that oleanolic acid might function as an allosteric agonist inducing clustering of αM on macrophages by shifting the balance from the closed to the extended-open conformation. The molecular target identified in this study might hold potential for a purposeful use of oleanolic acid to modulate chronic inflammatory responses.

scholarly journals Lycopene Inhibit IMQ-Induced Psoriasis-Like Inflammation by Inhibiting ICAM-1 Production in Mice

Polymers ◽  
2020 ◽  
Vol 12 (7) ◽  
pp. 1521
Author(s):  
Chun-Ming Shih ◽  
Chi-Kun Hsieh ◽  
Chien-Yu Huang ◽  
Chun-Yao Huang ◽  
Kuo-Hsien Wang ◽  
...  
Keyword(s):  
Adhesion Molecules ◽  
Inflammatory Responses ◽  
Critical Role ◽  
Intercellular Adhesion ◽  
Intercellular Adhesion Molecule ◽  
Intercellular Adhesion Molecule 1 ◽  
Intercellular Adhesion Molecules ◽  
Vitro Analysis ◽  
In Vitro Analysis

Lycopene is the most abundant carotenoid in tomatoes, which has been identified to have the properties of anti-inflammation in addition to the capability to inhibit the expression of adhesion molecules. Intercellular adhesion molecules play a critical role in the pathogenesis of psoriasis. Here, we report that the topical use of a lycopene decreased imiquimod (IMQ)-induced psoriasis-like inflammatory responses, the progress of which was based on adhesion molecules. In vitro analysis showed that lycopene decreased keratinocyte and monocyte adhesion. Evidence suggests that intercellular adhesion molecule-1 (ICAM-1) is a main mediator of psoriasis pathogenesis. Therefore, it will be interesting to investigate the factors that contribute to the lycopene-mediated inhibition of ICAM-1 expression in psoriasis. We expect that lycopene will with potential value in the treatment of psoriasis.

The native structure of the eukaryotic ribosome

1983 ◽  
Vol 41 ◽  
pp. 432-433
Author(s):  
R.A. Milligan ◽  
P.N.T. Unwin
Keyword(s):  
Ribosomal Proteins ◽  
Crystal Form ◽  
Native Structure ◽  
X Ray Diffraction ◽  
Eukaryotic Ribosome ◽  
Vitro Analysis ◽  
In Vitro Analysis ◽  
Resolution Structure ◽  
Stable Unit

A detailed understanding of the mechanism of protein synthesis will ultimately depend on knowledge of the native structure of the ribosome. Towards this end we have investigated the low resolution structure of the eukaryotic ribosome embedded in frozen buffer, making use of a system in which the ribosomes crystallize naturally.The ribosomes in the cells of early chicken embryos form crystalline arrays when the embryos are cooled at 4°C. We have developed methods to isolate the stable unit of these arrays, the ribosome tetramer, and have determined conditions for the growth of two-dimensional crystals in vitro, Analysis of the proteins in the crystals by 2-D gel electrophoresis demonstrates the presence of all ribosomal proteins normally found in polysomes. There are in addition, four proteins which may facilitate crystallization. The crystals are built from two oppositely facing P4 layers and the predominant crystal form, accounting for >80% of the crystals, has the tetragonal space group P4212, X-ray diffraction of crystal pellets demonstrates that crystalline order extends to ~ 60Å.

1163: Radial Dilation of Ureteral Balloons: A Comparative in-Vitro Analysis

2005 ◽  
Vol 173 (4S) ◽  
pp. 315-316
Author(s):  
Kari Hendlin ◽  
Brynn Lund ◽  
Manoj Monga
Keyword(s):  
Vitro Analysis ◽  
In Vitro Analysis

Role of the 807 C/T Polymorphism of the α2 Gene in Platelet GP Ia Collagen Receptor Expression and Function

1999 ◽  
Vol 81 (06) ◽  
pp. 951-956 ◽  
Author(s):  
J. Corral ◽  
R. González-Conejero ◽  
J. Rivera ◽  
F. Ortuño ◽  
P. Aparicio ◽  
...  
Keyword(s):  
Venous Thrombosis ◽  
Cell Types ◽  
Receptor Expression ◽  
Case Control Studies ◽  
Platelet Surface ◽  
Vitro Analysis ◽  
And Function ◽  
In Vitro Analysis

SummaryThe variability of the platelet GP Ia/IIa density has been associated with the 807 C/T polymorphism (Phe 224) of the GP Ia gene in American Caucasian population. We have investigated the genotype and allelic frequencies of this polymorphism in Spanish Caucasians. The T allele was found in 35% of the 284 blood donors analyzed. We confirmed in 159 healthy subjects a significant association between the 807 C/T polymorphism and the platelet GP Ia density. The T allele correlated with high number of GP Ia molecules on platelet surface. In addition, we observed a similar association of this polymorphism with the expression of this protein in other blood cell types. The platelet responsiveness to collagen was determined by “in vitro” analysis of the platelet activation and aggregation response. We found no significant differences in these functional platelet parameters according to the 807 C/T genotype. Finally, results from 3 case/control studies involving 302 consecutive patients (101 with coronary heart disease, 104 with cerebrovascular disease and 97 with deep venous thrombosis) determined that the 807 C/T polymorphism of the GP Ia gene does not represent a risk factor for arterial or venous thrombosis.

Sign in / Sign up

Export Citation Format

Share Document