scholarly journals Fiber Nanoarchitectonics for Pre-Treatments in Facile Detection of Short-Chain Fatty Acids in Waste Water and Faecal Samples

Polymers ◽  
2021 ◽  
Vol 13 (22) ◽  
pp. 3906
Author(s):  
Guozhe Deng ◽  
Li Xie ◽  
Shengjia Xu ◽  
Xuejun Kang ◽  
Jizheng Ma
Keyword(s):  
Waste Water ◽  
Fatty Acids ◽  
In Situ Polymerization ◽  
Short Chain Fatty Acids ◽  
Short Chain ◽  
Gas Chromatography Mass Spectrometry ◽  
Fecal Samples ◽  
Relative Standard ◽  
Chain Fatty Acids

Short-chain fatty acids (SCFAs) are among the active metabolites in biological process both in the intestinal tract and the bioconversion of organic wastes, which has resulted in various human diseases and environmental problems. In order to accurately detect SCFAs, we introduced a novel extraction sorbent. Electrospun polyacrylonitrile (PAN) nanofiber membrane was synthesized, then poly (3, 4-Ethylenedioxythiophene) (PEDOT) was deposited onto the surface of electrospun PAN nanofibers by in situ polymerization. The morphology of the composite PAN/PEDOT nanofiber was characterized by scanning electronic microscopy (SEM) and FTIR spectrum. PAN/PEDOT was used to isolate and concentrate the SCFAs in waste water and fecal samples before gas chromatography mass spectrometry (GC-MS) analysis. The analytical method was evaluated systematically, and low limits of detection (LODs) of 0.34–0.87 μg/L and good linearity (R2 ≥ 0.9953) were obtained. The method was applied successfully for the determination of SCFAs in waste water and fecal samples, with good recovery (87.5–104.6%) and satisfactory reproducibility (relative standard deviation: 6.5–14.1%). The results indicated that the proposed method can be used as a potential approach for the determination of SCFAs with high sensitivity in waste water and biological samples.

Freeze-drying enables homogeneous and stable sample preparation for determination of fecal short-chain fatty acids

2020 ◽  
Vol 589 ◽  
pp. 113508 ◽  
Author(s):  
Jun Ueyama ◽  
Masaya Oda ◽  
Masaaki Hirayama ◽  
Kuniyo Sugitate ◽  
Norihiro Sakui ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Sample Preparation ◽  
Freeze Drying ◽  
Short Chain Fatty Acids ◽  
Short Chain ◽  
Chain Fatty Acids

Quantitative Gas-Chromatographic Determination of Short-Chain Fatty Acids in Aqueous Samples

1974 ◽  
Vol 20 (9) ◽  
pp. 1235-1237 ◽  
Author(s):  
Dennis P Collin ◽  
Patrick G McCormick ◽  
Milton G Schmitt
Keyword(s):  
Fatty Acids ◽  
Free Acid ◽  
Internal Standard ◽  
Short Chain Fatty Acids ◽  
Chromatographic Determination ◽  
Short Chain ◽  
Aqueous Samples ◽  
Peak Areas ◽  
Chain Fatty Acids

Abstract We report the use of SP-1200 (Supelco Inc.) for quantitative gas-chromatographic determination of short-chain fatty acids (C2-C5) in stool water. The ratio of peak areas for these acids to that for 2-methylvaleric acid (internal standard) is linear for each acid from 60 to 1800 mg/liter. Lactic acid, occasionally present in stool in abnormally high amounts, is not detectable as the free acid on SP-1200, but is determined as its propyl ester on diethyleneglycolsuccinate.

Application of a microbial sensor for determination of short-chain fatty acids in raw milk samples

1992 ◽  
Vol 195 (1) ◽  
pp. 1-2 ◽  
Author(s):  
Hiroyuki Ukeda ◽  
Gotthold Wagner ◽  
G�nther Weis ◽  
Manfred Miller ◽  
Henning Klostermeyer ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Raw Milk ◽  
Short Chain Fatty Acids ◽  
Short Chain ◽  
Milk Samples ◽  
Microbial Sensor ◽  
Chain Fatty Acids

scholarly journals Determination of Short-Chain Fatty Acids in Raw Milk Using a Microbial Sensor and the Relationship with Milk Quality.

1994 ◽  
Vol 10 (4) ◽  
pp. 683-685 ◽  
Author(s):  
Hiroyuki UKEDA ◽  
Yoshihiro FUJITA ◽  
Masayoshi SAWAMURA ◽  
Hirozo KUSUNOSE
Keyword(s):  
Fatty Acids ◽  
Raw Milk ◽  
Short Chain Fatty Acids ◽  
Milk Quality ◽  
Short Chain ◽  
Microbial Sensor ◽  
The Relationship ◽  
Chain Fatty Acids

scholarly journals Associations between disordered gut microbiota and changes of neurotransmitters and short-chain fatty acids in depressed mice

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Min Wu ◽  
Tian Tian ◽  
Qiang Mao ◽  
Tao Zou ◽  
Chan-juan Zhou ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Acetic Acid ◽  
Gut Microbiota ◽  
Molecular Mechanisms ◽  
Short Chain Fatty Acids ◽  
Short Chain ◽  
Rrna Gene ◽  
Microbial Composition ◽  
Fecal Samples ◽  
Chain Fatty Acids

Abstract Mounting evidence suggests that gut microbiota can play an important role in pathophysiology of depression, but its specific molecular mechanisms are still unclear. This study was conducted to explore the associations between changes in neurotransmitters and short-chain fatty acids (SCFAs) and altered gut microbiota in depressed mice. Here, the chronic restraint stress (CRS) model of depression was built. The classical behavioral tests were conducted to assess the depressive-like behaviors of mice. The 16S rRNA gene sequence extracted from fecal samples was used to assess the gut microbial composition. Liquid and gas chromatography mass spectroscopy were used to identify neurotransmitters in hypothalamus and SCFAs in fecal samples, respectively. Finally, 29 differential bacteria taxa between depressed mice and control mice were identified, and the most differentially abundant bacteria taxa were genus Allobaculum and family Ruminococcaceae between the two groups. The acetic acid, propionic acid, pentanoic acid, norepinephrine, 5-HIAA and 5-HT were significantly decreased in depressed mice compared to control mice. Genus Allobaculum was found to be significantly positively correlated with acetic acid and 5-HT. Taken together, these results provided novel microbial and metabolic frameworks for understanding the role of microbiota-gut-brain axis in depression, and suggested new insights to pave the way for novel therapeutic methods.

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