scholarly journals Porous Bilayer Vascular Grafts Fabricated from Electrospinning of the Recombinant Human Collagen (RHC) Peptide-Based Blend

Polymers ◽  
2021 ◽  
Vol 13 (22) ◽  
pp. 4042
Author(s):  
Thi My Do ◽  
Yang Yang ◽  
Aipeng Deng
Keyword(s):  
Outer Layer ◽  
Mechanical Test ◽  
Umbilical Vein ◽  
Vascular Grafts ◽  
Water Soluble ◽  
Causes Of Mortality ◽  
Human Collagen ◽  
Nanoscale Fibers ◽  
Umbilical Vein Endothelial Cells

Cardiovascular diseases, including coronary artery and peripheral vascular pathologies, are leading causes of mortality. As an alternative to autografts, prosthetic grafts have been developed to reduce the death rate. This study presents the development and characterization of bilayer vascular grafts with appropriate structural and biocompatibility properties. A polymer blend of recombinant human collagen (RHC) peptides and polycaprolactone (PCL) was used to build the inner layer of the graft by electrospinning and co-electrospinning the water-soluble polyethylene oxide (PEO) as sacrificial material together with PCL to generate the porous outer layer. The mechanical test demonstrated the bilayer scaffold’s appropriate mechanical properties as compared with the native vascular structure. Human umbilical vein endothelial cells (HUVEC) showed enhanced adhesion to the lumen after seeding on nanoscale fibers. Meanwhile, by enhancing the porosity of the microfibrous outer layer through the removal of PEO fibers, rat smooth muscle cells (A7r5) could proliferate and infiltrate the porous layer easily.

Partial Characterization of a Fibrinolytic Inhibitor Produced by Cultured Endothelial Cells Derived from Human Umbilical Vein

1982 ◽  
Vol 47 (02) ◽  
pp. 128-131 ◽  
Author(s):  
F Esnard ◽  
E Dupuy ◽  
A M Dosne ◽  
E Bodevin
Keyword(s):  
Endothelial Cells ◽  
Primary Culture ◽  
Ammonium Sulphate ◽  
Concanavalin A ◽  
Umbilical Vein ◽  
Human Umbilical Vein ◽  
Preliminary Characterization ◽  
Umbilical Vein Endothelial Cells ◽  
Ammonium Sulphate Saturation

SummaryA preliminary characterization of a fibrinolytic inhibitor released by human umbilical vein endothelial cells in primary culture is reported. This molecule of Mr comprised between 2 × 105 and 106 and of μ2 mobility precipitates at 43% ammonium sulphate saturation and is totally adsorbed on Concanavalin A Sepharose 4 B. A possible relationship with a macroglobulins is discussed.

scholarly journals Characterization of the interactions between procoagulant albumin and human endothelial cells

Blood ◽  
1993 ◽  
Vol 82 (9) ◽  
pp. 2684-2692
Author(s):  
KJ Faucette ◽  
LA Fitzgerald ◽  
L Liu ◽  
CJ Parker ◽  
GM Rodgers
Keyword(s):  
Endothelial Cells ◽  
Umbilical Vein ◽  
Quantitative Polymerase Chain Reaction ◽  
Scavenger Receptor ◽  
Kinase C ◽  
Normal Human ◽  
Membrane Bound ◽  
Polymerase Chain ◽  
Umbilical Vein Endothelial Cells

Normal human plasma contains procoagulant albumin (PC-Al), an anionic form of albumin that induces tissue factor (TF) activity in human umbilical vein endothelial cells (HUVEC) and monocytes. In this study, we investigated both the interactions between HUVEC and PC-Al and the mechanism by which PC-Al induces TF activity. Binding of PC-Al to HUVEC was specific and reversible. Further studies indicated that membrane- bound PC-Al was not internalized by HUVEC. A potential receptor on HUVEC was suggested by studies in which the capacity of a variety of reagents to inhibit the activity of PC-Al was quantitated. Induction of TF activity by PC-Al was antagonized by dextran sulfate, heparin, fucoidan, and concanavalin A but not by ovalbumin, polyglutamic acid, or polyvinyl sulfate. This competition profile bears similarities to those reported for scavenger receptors that have been identified on both HUVEC and monocytes. Involvement of protein kinase C (PKC) in the PC-Al-induced enhancement of TF activity was suggested by experiments in which staurosporine, an inhibitor of PKC, suppressed the activity of PC-Al. The induction of TF activity by PC-Al was further characterized by using a quantitative polymerase chain reaction assay. Increased TF mRNA was first seen after 1 hour of incubation with PC-Al. Maximal observed expression occurred at 2 hours, but at 5 hours, expression had significantly decreased. Monocytes could also be induced to express TF mRNA after a 2-hour incubation with PC-Al. These results suggest that the functionally relevant binding of PC-Al to HUVEC may be mediated through interactions with a membrane constituent that has some of the properties of a scavenger receptor and that this interaction augments TF activity by enhancing transcription of TF mRNA, at least in part, by a mechanism that is dependent on activation of PKC.

scholarly journals Parallel Isolation and Characterization of Porcine Smooth Muscle, Endothelial and Mesenchymal Stromal Cells for Bioengineering Applications

2021 ◽  
Author(s):  
Sara Morini ◽  
Iris Pla-Palacín ◽  
Pilar Sainz-Arnal ◽  
Natalia Sánchez-Romero ◽  
Maria Falceto ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Cultured Cells ◽  
Umbilical Vein ◽  
Building Blocks ◽  
Cell Types ◽  
Aortic Smooth Muscle ◽  
Isolation And Characterization ◽  
Umbilical Vein Endothelial Cells

Abstract There is significant interest in the pig as the animal model of choice for organ transplantation and the study of tissue engineering (TE) products and applications. Currently, efforts are being taken to bioengineer solid organs to reduce donor shortages for transplantation. For complex organs such as the lung, heart, and liver, the vasculature represents a fundamental feature. Thus, to generate organs with a functional vascular network, the different cells constituting the building blocks of the blood vessels should be procured. However, due to species' specificities, porcine cell isolation, expansion, and characterization are not entirely straightforward compared to human cell procurement. Here, we report the establishment of simple and suitable methods for the isolation and characterization of distinct porcine cells for bioengineering purposes.We successfully isolated, expanded and characterized porcine bone marrow-derived mesenchymal stromal (pBM-MSC), aortic smooth muscle (pASMC), and umbilical vein endothelial cells (pUVEC). We demonstrated that the three cell types showed specific immunophenotypical features. Moreover, we demonstrated that pBM-MSC could preserve their multipotency in vitro, and pUVEC were capable of maintaining their functionality in vitro.These cultured cells could be further expanded and represent a useful cellular tool for TE purposes (i.e., for recellularization approaches of vascularized organs or in vitro angiogenesis studies).

Proteomic characterization of oxidative dysfunction in human umbilical vein endothelial cells (HUVEC) induced by exposure to oxidized LDL

2005 ◽  
Vol 39 (12) ◽  
pp. 1335-1344 ◽  
Author(s):  
Tomoya Kinumi ◽  
Yoko Ogawa ◽  
Junko Kimata ◽  
Yoshiro Saito ◽  
Yasukazu Yoshida ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Umbilical Vein ◽  
Oxidized Ldl ◽  
Human Umbilical Vein ◽  
Umbilical Vein Endothelial Cells

Characterization of Gastrin-Induced Proangiogenic Effects In vivo in Orthotopic U373 Experimental Human Glioblastomas and In vitro in Human Umbilical Vein Endothelial Cells

2004 ◽  
Vol 10 (24) ◽  
pp. 8250-8265 ◽  
Author(s):  
Florence Lefranc ◽  
Tatjana Mijatovic ◽  
Véronique Mathieu ◽  
Sandrine Rorive ◽  
Christine Decaestecker ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Umbilical Vein ◽  
Human Umbilical Vein ◽  
Umbilical Vein Endothelial Cells

scholarly journals Imaging specific cellular glycan structures using glycosyltransferases via click chemistry

2017 ◽  
Author(s):  
Zhengliang L Wu ◽  
Anthony Person ◽  
Matthew Anderson ◽  
Barbara Burroughs ◽  
Timothy Tatge ◽  
...  
Keyword(s):  
Umbilical Vein ◽  
Functional Characterization ◽  
Tn Antigen ◽  
Stem Cell Line ◽  
Rate Limiting Step ◽  
Architectural Features ◽  
Huvec Cells ◽  
Umbilical Vein Endothelial Cells ◽  
Rate Limiting

AbstractHeparan sulfate (HS) is a polysaccharide fundamentally important for biologically activities. T/Tn antigens are universal carbohydrate cancer markers. Here, we report the specific imaging of these carbohydrates using a mesenchymal stem cell line and human umbilical vein endothelial cells (HUVEC). The staining specificities were demonstrated by comparing imaging of different glycans and validated by either removal of target glycans, which results in loss of signal, or installation of target glycans, which results in gain of signal. As controls, representative key glycans including O-GlcNAc, lactosaminyl glycans and hyaluronan were also imaged. HS staining revealed novel architectural features of the extracellular matrix (ECM) of HUVEC cells. Results from T/Tn antigen staining suggest that O-GalNAcylation is a rate-limiting step for O-glycan synthesis. Overall, these highly specific approaches for HS and T/Tn antigen imaging should greatly facilitate the detection and functional characterization of these biologically important glycans.

scholarly journals Bioprinting small-diameter vascular vessel with endothelium and smooth muscle by the approach of two-step crosslinking process

2021 ◽  
Author(s):  
Qianheng Jin ◽  
Guangzhe Jin ◽  
Jihui Ju ◽  
Lei Xu ◽  
Linfeng Tang ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Uv Light ◽  
Umbilical Vein ◽  
Vascular Grafts ◽  
Three Dimensional ◽  
Small Diameter ◽  
Tubular Structure ◽  
Flat Structure ◽  
Crosslinking Process ◽  
Umbilical Vein Endothelial Cells

Three-dimensional (3D) bioprinting shows great potential for autologous vascular grafts due to its simplicity, accuracy, and flexibility. 6mm diameter vascular grafts are used in clinic. However, producing small-diameter vascular grafts are still an enormous challenge. Normally, sacrificial hydrogels are used as temporary lumen support to mold tubular structure which will affect the structure’s stability. In this study, we develop a new bioprinting approach to fabricating small-diameter vessel using two-step crosslinking process. ¼ lumen wall of bioprinted gelatin mechacrylate (GelMA) flat structure is exposed to ultraviolet (UV) light briefly for having certain strength, while ¾ lumen wall shows as concave structure remained uncrosslinked. Pre-crosslinked flat structure is merged towards the uncrosslinked concave structure. Two individual structures will be combined tightly into an intact tubular structure by receiving more UV exposure time. Complicated tubular structures are constructed by these method. Notably, the GelMA-based bioink loaded with smooth muscle cells (SMCs) are bioprinted as the outer layer and human umbilical vein endothelial cells (HUVECs) are seeded onto the inner surface. A bionic vascular vessel with dual layers is fabricated successfully and keeps good viability, and functionality. This study may provide a novel idea for fabricating biomimetic vascular network or other more complicated organs.

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