Chemical Profiling of Pistacia lentiscus var. Chia Resin and Essential Oil: Ageing Markers and Antimicrobial Activity

Chios Mastic Gum (CMG) and Chios Mastic Oil (CMO) are two unique products of the tree Pistacia lentiscus var. Chia, cultivated exclusively on the Greek island of Chios. In the present study, the method proposed by the European Pharmacopoeia for mastic identification was employed using HPTLC together with an in-house method. A GC-MS methodology was also developed for the chemical characterization of CMOs. α-Pinene and β-myrcene were found in abundance in the fresh oils; however, in the oil of the aged collection, oxygenated monoterpenes and benzenoids such as verbenone, pinocarveol, and α-campholenal were found at the highest rates. Additionally, the antimicrobial activity of Chios Mastic Gums (CMGs) with their respective Chios Mastic Oils (CMOs) was evaluated, with growth tests against the fungi Aspergillus nidulans, Aspergillus fumigatus, Candida albicans, Mucor circinelloides, and Rhizopus oryzae, and the bacteria Escherichia coli, Pseudomonas aeruginosa and Bacillus subtilis, with the samples exhibiting a moderate activity. To our knowledge, this is the first time that an HPTLC method is proposed for the analysis of mastic and its essential oil and that a standardized methodology is followed for the distillation of CMO with a parallel assessment of the ageing effect on the oil’s composition.

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Chemical profiling and antimicrobial activity of essential oil from Curcuma aeruginosa Roxb., Curcuma glans K. Larsen & J. Mood and Curcuma cf. xanthorrhiza Roxb. collected in Thailand

Asian Pacific Journal of Tropical Biomedicine ◽

10.1016/j.apjtb.2017.09.009 ◽

2017 ◽

Vol 7 (10) ◽

pp. 881-885 ◽

Cited By ~ 16

Author(s):

Nararat Akarchariya ◽

Sasithorn Sirilun ◽

Jakaphun Julsrigival ◽

Sunee Chansakaowa

Keyword(s):

Antimicrobial Activity ◽

Essential Oil ◽

Chemical Profiling

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Antimicrobial activity of the essential oil of mastic gum (Pistacia lentiscus var. chia) on Gram positive and Gram negative bacteria in broth and in Model Food System

International Biodeterioration & Biodegradation ◽

10.1016/0964-8305(95)00103-4 ◽

1995 ◽

Vol 36 (3-4) ◽

pp. 411-420 ◽

Cited By ~ 49

Author(s):

Chrysoula C. Tassou ◽

G.J.E. Nychas

Keyword(s):

Antimicrobial Activity ◽

Essential Oil ◽

Food System ◽

Gram Negative Bacteria ◽

Pistacia Lentiscus ◽

Gram Positive ◽

Gram Negative ◽

Model Food

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Chemical characterization, antioxidant capacity and antimicrobial activity against food related microorganisms of Citrus limon var. pompia leaf essential oil

LWT ◽

10.1016/j.lwt.2016.02.018 ◽

2016 ◽

Vol 69 ◽

pp. 579-585 ◽

Cited By ~ 25

Author(s):

Francesco Fancello ◽

Giacomo Luigi Petretto ◽

Severino Zara ◽

Maria Lina Sanna ◽

Roberta Addis ◽

...

Keyword(s):

Antimicrobial Activity ◽

Essential Oil ◽

Antioxidant Capacity ◽

Chemical Characterization ◽

Citrus Limon ◽

Leaf Essential Oil

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ANTIMICROBIAL ACTIVITY OF ESSENTIAL OIL OF PISTACIA LENTISCUS GROWING IN FOREST OF BOUMERDES

14th SGEM GeoConference on WATER RESOURCES. FOREST, MARINE AND OCEAN ECOSYSTEMS ◽

10.5593/sgem2014/b32/s14.042 ◽

2014 ◽

Author(s):

Nadjiba Meziou Chebouti

Keyword(s):

Antimicrobial Activity ◽

Essential Oil ◽

Pistacia Lentiscus

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Chemical Characterization, Antioxidant, Cytotoxic and Microbiological Activities of the Essential Oil of Leaf of Tithonia Diversifolia (Hemsl) A. Gray (Asteraceae)

Pharmaceuticals ◽

10.3390/ph12010034 ◽

2019 ◽

Vol 12 (1) ◽

pp. 34 ◽

Cited By ~ 2

Author(s):

Ana Ferreira Farias ◽

Alex Lobato Rodrigues ◽

Rosany Lopes Martins ◽

Érica de Menezes Rabelo ◽

Carlos Ferreira Farias ◽

...

Keyword(s):

Antimicrobial Activity ◽

Essential Oil ◽

Cytotoxic Activity ◽

Chemical Constituents ◽

Larval Mortality ◽

Chemical Characterization ◽

Artemia Salina ◽

Antioxidant Potential ◽

Tithonia Diversifolia ◽

Bacterial Strains

The present study aimed to evaluate the chemical composition, antioxidant potential, and the cytotoxic and antimicrobial activity of the essential oil of the plant species Tithonia diversifolia (Hemsl) A. Gray. The essential oil obtained was used to identify the chemical compounds present through the techniques of GC-MS and NMR. The antioxidant potential was calculated by the sequestration method of 2,2-diphenyl-1-picrylhydrazyl. For cytotoxic activity, the larval mortality of Artemia salina was evaluated. The main chemical constituents identified are αpinene (9.9%), Limonene (5.40%), (Z)-β-ocimene (4.02%), p-cymen-8-ol (3.0%), Piperitone (11.72%), (E)-nerolidol (3.78%) and Spathulenol (10.8%). In the evaluation of the antimicrobial activity, bacterial strains of Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa were used. The results showed that the bacterium E. coli were more susceptible to the presence of the essential oil, presenting minimal inhibitory concentration at the concentrations that were exposed. The essential oil presented antioxidant activity of 54.6% at the concentration of 5 mg·mL−1 and provided a CI50 of 4.30. It was observed that the essential oil of this species was highly toxic against A. salina lavas, as its cytotoxic activity showed an LC50 of 3.11. Thus, it is concluded that T. diversifolia oils are effective in inhibiting bacterial growth and reducing oxidative stress.

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Bio-Guided Fractionation of Prenylated Benzaldehyde Derivatives as Potent Antimicrobial and Antibiofilm from Ammi majus L. Fruits-Associated Aspergillus amstelodami

Molecules ◽

10.3390/molecules24224118 ◽

2019 ◽

Vol 24 (22) ◽

pp. 4118 ◽

Cited By ~ 5

Author(s):

Noha Fathallah ◽

Marwa M. Raafat ◽

Marwa Y. Issa ◽

Marwa M. Abdel-Aziz ◽

Mokhtar Bishr ◽

...

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Antimicrobial Activity ◽

Streptococcus Mutans ◽

Endophytic Fungus ◽

Inhibitory Concentration ◽

Antibiofilm Activity ◽

Moderate Activity ◽

Ammi Majus ◽

First Time

Ammi majus L.; Family Apiaceae; is a plant indigenous to Egypt. Its fruits contain bioactive compounds such as furanocoumarins and flavonoids of important biological activities. An endophytic fungus was isolated from the fruits and identified as Aspergillus amstelodami (MK215708) by morphology, microscopical characterization, and molecular identification. To our knowledge this is the first time an endophytic fungus has been isolated from the fruits. The antimicrobial activity of the Ammi majus ethanol fruits extract (AME) and fungal ethyl acetate extract (FEA) were investigated, where the FEA showed higher antimicrobial activity, against all the tested standard strains. Phytochemical investigation of the FEA extract yielded five prenylated benzaldehyde derivative compounds isolated for the first time from this species: Dihydroauroglaucin (1), tetrahydroauroglaucin (2), 2-(3,6-dihydroxyhepta-1,4-dien-1-yl)-3,6-dihydroxy-5-(dimethylallyl)benzaldehyde (3), isotetrahydroauroglaucin )4), and flavoglaucin (5). Structure elucidation was carried out using (1H- and 13C-NMR). Fractions and the major isolated compound 1 were evaluated for their antimicrobial and antibiofilm activity. Compound 1 showed high antimicrobial activity against Escherichia coli with minimum inhibitory concentration (MIC) = 1.95 µg/mL, Streptococcus mutans (MIC = 1.95 µg/mL), and Staphylococcus aureus (MIC = 3.9 µg/mL). It exhibited high antibiofilm activity with minimum biofilm inhibitory concentration (MBIC) = 7.81 µg/mL against Staphylococcus aureus and Escherichia coli biofilms and MBIC = 15.63 µg/mL against Streptococcus mutans and Candida albicans and moderate activity (MBIC = 31.25 µg/mL) against Pseudomonas aeruginosa biofilm. This reveals that dihydroauroglaucin, a prenylated benzaldehyde derivative, has a broad spectrum antimicrobial activity. In conclusion, it was observed that the MICs of the FEA are much lower than that of the AME against all susceptible strains, confirming that the antimicrobial activity of Ammi majus may be due to the ability of its endophytic fungi to produce effective secondary metabolites.

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Composition and Antimicrobial Activity of Achillea Distans Essential Oil

Natural Product Communications ◽

10.1177/1934578x1100600635 ◽

2011 ◽

Vol 6 (6) ◽

pp. 1934578X1100600 ◽

Cited By ~ 3

Author(s):

Angel Konakchiev ◽

Milka Todorova ◽

Bozhanka Mikhova ◽

Antonina Vitkova ◽

Hristo Najdenski

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Antimicrobial Activity ◽

Candida Albicans ◽

Essential Oil ◽

Salmonella Typhimurium ◽

Moderate Activity ◽

Proteus Vulgaris ◽

Oil Composition ◽

Weak Activity

The essential oil of Achillea distans W. et K. flower heads was analyzed by GC and GC-MS. Altogether 43 components in concentrations more than 0.1% were identified representing 93.5% of the oil composition. The main constituents were 1,8-cineole (16.8%), trans-thujone (9.8%), sabinene (8.2%), borneol (7.5%), β-pinene (6.5%), and camphor (5.8%). The oil showed moderate activity against Staphylococcus aureus and Candida albicans, and weak activity against Salmonella typhimurium, Proteus vulgaris, and Escherichia coli.

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