Effects of Adding Laccase to Bacterial Consortia Degrading Heavy Oil

High-efficiency bioremediation technology for heavy oil pollution has been a popular research topic in recent years. Laccase is very promising for the remediation of heavy oil pollution because it can not only convert bio-refractory hydrocarbons into less toxic or completely harmless compounds, but also accelerate the biodegradation efficiency of heavy oil. However, there are few reports on the use of laccase to enhance the biodegradation of heavy oil. In this study, we investigated the effect of laccase on the bacterial consortia degradation of heavy oil. The degradation efficiencies of bacterial consortia and the laccase-bacterial consortia were 60.6 ± 0.1% and 68.2 ± 0.6%, respectively, and the corresponding heavy oil degradation rate constants were 0.112 day−1 and 0.198 day−1, respectively. The addition of laccase increased the heavy oil biodegradation efficiency (p < 0.05) and biodegradation rate of the bacterial consortia. Moreover, gas chromatography–mass spectrometry analysis showed that the biodegradation efficiencies of the laccase-bacterial consortia for saturated hydrocarbons and aromatic hydrocarbons were 82.5 ± 0.7% and 76.2 ± 0.9%, respectively, which were 16.0 ± 0.3% and 13.0 ± 1.8% higher than those of the bacterial consortia, respectively. In addition, the degradation rate constants of the laccase-bacterial consortia for saturated hydrocarbons and aromatic hydrocarbons were 0.267 day−1 and 0.226 day−1, respectively, which were 1.07 and 1.15 times higher than those of the bacterial consortia, respectively. The degradation of C15 to C35 n-alkanes and 2 to 5-ring polycyclic aromatic hydrocarbons by laccase-bacterial consortia was higher than individual bacterial consortia. It is further seen that the addition of laccase significantly improved the biodegradation of long-chain n-alkanes of C22–C35 (p < 0.05). Overall, this study shows that the combination of laccase and bacterial consortia is an effective remediation technology for heavy oil pollution. Adding laccase can significantly improve the heavy oil biodegradation efficiency and biodegradation rate of the bacterial consortia.

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Potential in heavy oil biodegradation via enrichment of spore forming bacterial consortia

Journal of Petroleum Exploration and Production Technology ◽

10.1007/s13202-016-0228-8 ◽

2016 ◽

Vol 6 (4) ◽

pp. 787-799 ◽

Cited By ~ 6

Author(s):

Saif N. Al-Bahry ◽

Yahya M. Al-Wahaibi ◽

Balqees Al-Hinai ◽

Sanket J. Joshi ◽

Abdulkadir E. Elshafie ◽

...

Keyword(s):

Heavy Oil ◽

Bacterial Consortia ◽

Oil Biodegradation

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New strategy to increase oil biodegradation efficiency by selecting isolates with diverse functionality and no antagonistic interactions for bacterial consortia

Journal of Environmental Chemical Engineering ◽

10.1016/j.jece.2021.106315 ◽

2021 ◽

pp. 106315

Author(s):

Ali Ebadi ◽

Akbar Ghavidel ◽

Nayer Azam Khoshkholgh Sima ◽

Ghasem Heydari ◽

Mohammad Reza Gaffari

Keyword(s):

Bacterial Consortia ◽

Oil Biodegradation ◽

New Strategy ◽

Antagonistic Interactions ◽

Biodegradation Efficiency

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Corrigendum to ‘New strategy to increase oil biodegradation efficiency by selecting isolates with diverse functionality and no antagonistic interactions for bacterial consortia’ [J. Environ. Chem. Eng. 9(5) (2021) 106315]

Journal of Environmental Chemical Engineering ◽

10.1016/j.jece.2021.106535 ◽

2021 ◽

Vol 9 (6) ◽

pp. 106535

Author(s):

Ali Ebadi ◽

Akbar Ghavidel ◽

Nayer Azam Khoshkholgh Sima ◽

Ghasem Heydari ◽

Mohammad Reza Ghaffari

Keyword(s):

Bacterial Consortia ◽

Oil Biodegradation ◽

New Strategy ◽

Antagonistic Interactions ◽

Biodegradation Efficiency

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Quantifying biodegradation rate constants of o-xylene by combining compound-specific isotope analysis and groundwater dating

Journal of Contaminant Hydrology ◽

10.1016/j.jconhyd.2020.103757 ◽

2021 ◽

pp. 103757

Author(s):

Andreas Würth ◽

Kathrin Menberg ◽

Peter Martus ◽

Jürgen Sültenfuß ◽

Philipp Blum

Keyword(s):

Rate Constants ◽

Isotope Analysis ◽

Biodegradation Rate ◽

Compound Specific Isotope Analysis ◽

Groundwater Dating

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Production of High Purity Biosurfactants Using Heavy Oil Residues as Carbon Source

Energies ◽

10.3390/en14123557 ◽

2021 ◽

Vol 14 (12) ◽

pp. 3557

Author(s):

Athina Mandalenaki ◽

Nicolas Kalogerakis ◽

Eleftheria Antoniou

Keyword(s):

Carbon Source ◽

Heavy Oil ◽

Oil Pollution ◽

Batch Reactor ◽

Production Method ◽

Promising Alternative ◽

Heavy Oil Residues ◽

Oil Residue ◽

Increasing Demand ◽

Heavy Oil Residue

Typically, oil pollution cleanup procedures following first response actions include dispersion. Crude oil is biodegradable, and its bioavailability can be increased when dispersed into very fine droplets by means of chemical surfactants. Although their use is widely spread in many applications, the latter may prove toxic, depending on the extent of use. The use of biological means, such as bioremediation and biosurfactants, has emerged over the past years as a very promising ‘green’ alternative technology. Biosurfactants (BSs) are amphiphilic molecules produced by microorganisms during biodegradation, thus increasing the bioavailability of the organic pollutants. It is their biodegradability and low toxicity that render BSs as a very promising alternative to the synthetic ones. Alcanivorax borkumensis SK2 strain ability to produce BSs, without any impurities from the substrate, was investigated. The biosurfactant production was scaled up by means of a sequencing batch reactor (SBR) and a heavy oil residue substrate as the carbon source. The product is free from substrate impurities, and its efficiency is tested on oil bioremediation in the marine environment. The product’s dispersion efficiency was determined by the baffled flask test. The production method proposed can have a significant impact to the market, given the ever-increasing demand for ecologically friendly, reliable, commercially viable and economically competitive environmental cleanup techniques.

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The Role of H2C2O4 and Na2CO3 as Precipitating Agents on The Physichochemical Properties and Photocatalytic Activity of Bismuth Oxide

Open Chemistry ◽

10.1515/chem-2020-0013 ◽

2020 ◽

Vol 18 (1) ◽

pp. 129-137

Author(s):

Yayuk Astuti ◽

Rizka Andianingrum ◽

Abdul Haris ◽

Adi Darmawan ◽

Keyword(s):

Photocatalytic Activity ◽

Methyl Orange ◽

Physicochemical Properties ◽

Bismuth Oxide ◽

Rate Constants ◽

Degradation Rate ◽

Precipitation Method ◽

Methyl Orange Degradation ◽

Methyl Orange Dye

AbstractSynthesis of bismuth oxide synthesis through the precipitation method using H2C2O4 and Na2CO3 precipitating agents, identification of physicochemical properties and its photocatalysis activity for methyl orange degradation were conducted. The bismuth oxide synthesis was undertaken by dissolving Bi(NO3)3.5H2O in HNO3, then added precipitating agents to form precipitate. The results showed that bismuth oxide produced by H2C2O4 precipitating agent was a yellow powder containing a mixture of α-Bi2O3 (monoclinic) and β-Bi2O3 (tetragonal), porous with size of 28-85 μm. Meanwhile, the use of Na2CO3 as precipitating agent resulted in bismuth oxide consisting of α-Bi2O3 and β-Bi2O3 and Bi2O4, irregular shape without pore being 40-115 μm in size. Bismuth oxide synthesized with H2C2O4 precipitating agent showed higher photocatalytic activity compared to bismuth oxide synthesized using Na2CO3 on degrading methyl orange dye with degradation rate constants of 2.35x10-5 s-1 for H2C2O4 and 1.81x10-5 s-1 for Na2CO3.

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Biodegradation of Used Motor Oil in Soil Using Organic Waste Amendments

Biotechnology Research International ◽

10.1155/2012/587041 ◽

2012 ◽

Vol 2012 ◽

pp. 1-8 ◽

Cited By ~ 43

Author(s):

O. P. Abioye ◽

P. Agamuthu ◽

A. R. Abdul Aziz

Keyword(s):

Contaminated Soil ◽

Oil Pollution ◽

Green Technology ◽

Lubricating Oil ◽

Soil Bioremediation ◽

Order Kinetic ◽

Human Beings ◽

Mushroom Compost ◽

Oil Biodegradation ◽

Used Lubricating Oil

Soil and surface water contamination by used lubricating oil is a common occurrence in most developing countries. This has been shown to have harmful effects on the environment and human beings at large. Bioremediation can be an alternative green technology for remediation of such hydrocarbon-contaminated soil. Bioremediation of soil contaminated with 5% and 15% (w/w) used lubricating oil and amended with 10% brewery spent grain (BSG), banana skin (BS), and spent mushroom compost (SMC) was studied for a period of 84 days, under laboratory condition. At the end of 84 days, the highest percentage of oil biodegradation (92%) was recorded in soil contaminated with 5% used lubricating oil and amended with BSG, while only 55% of oil biodegradation was recorded in soil contaminated with 15% used lubricating oil and amended with BSG. Results of first-order kinetic model to determine the rate of biodegradation of used lubricating oil revealed that soil amended with BSG recorded the highest rate of oil biodegradation (0.4361 day−1) in 5% oil pollution, while BS amended soil recorded the highest rate of oil biodegradation (0.0556 day−1) in 15% oil pollution. The results of this study demonstrated the potential of BSG as a good substrate for enhanced remediation of hydrocarbon contaminated soil at low pollution concentration.

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