scholarly journals The Role of Dactylis Glomerata and Diesel Oil in the Formation of Microbiome and Soil Enzyme Activity

Sensors ◽  
2020 ◽  
Vol 20 (12) ◽  
pp. 3362
Author(s):  
Agata Borowik ◽  
Jadwiga Wyszkowska ◽  
Mirosław Kucharski ◽  
Jan Kucharski
Keyword(s):  
Soil Pollution ◽  
Diversity Index ◽  
Dactylis Glomerata ◽  
Diesel Oil ◽  
Petroleum Products ◽  
Gasoline Fraction ◽  
Polluted Soil ◽  
Soil Microbiome ◽  
Core Microbiome ◽  
Bacterial Structure

The global demand for petroleum contributes to a significant increase in soil pollution with petroleum-based products that pose a severe risk not only to humans but also to plants and the soil microbiome. The increasing pollution of the natural environment urges the search for effective remediation methods. Considering the above, the objective of this study was to determine the usability of Dactylis glomerata for the degradation of hydrocarbons contained in diesel oil (DO), as well as the effects of both the plant tested and DO on the biochemical functionality and changes in the soil microbiome. The experiment was conducted in a greenhouse with non-polluted soil as well as soil polluted with DO and phytoremediated with Dactylis glomerata. Soil pollution with DO increased the numbers of microorganisms and soil enzymes and decreased the value of the ecophysiological diversity index of microorganisms. Besides, it contributed to changes in the bacterial structure at all taxonomic levels. DO was found to increase the abundance of Proteobacteria and to decrease that of Actinobacteria, Acidobacteria, Chloroflexi, Gemmatimonadetes and Firmicutes. In the non-polluted soil, the core microbiome was represented by Kaistobacter and Rhodoplanes, whereas in the DO-polluted soil, it was represented by Parvibaculum and Rhodococcus. In soil sown with Dactylis glomerata, gasoline fraction (C6–C12) degradation was higher by 17%; mineral oil (C12–C35), by 9%; benzene, by 31%; anthracene, by 12%; chrysene, by 38%; benzo(a)anthracene, by 19%; benzo(a)pyrene, by 17%; benzo(b)fluoranthene, by 15%; and benzo(k)fluoranthene, by 18% than in non-sowed soil. To conclude, Dactylis glomerata proved useful in degrading DO hydrocarbons and, therefore, may be recommended for the phytoremediation of soils polluted with petroleum-based products. It has been shown that the microbiological, biochemical and chemical tests are fast and sensitive in the diagnosis of soil contamination with petroleum products, and a combination of all these tests gives a reliable assessment of the state of soils.

scholarly journals Microbiological Study in Petrol-Spiked Soil

Molecules ◽  
2021 ◽  
Vol 26 (9) ◽  
pp. 2664
Author(s):  
Agata Borowik ◽  
Jadwiga Wyszkowska ◽  
Jan Kucharski
Keyword(s):  
Soil Pollution ◽  
Soil Microorganisms ◽  
Diversity Index ◽  
Biochemical Properties ◽  
Polluted Soil ◽  
Hydrocarbon Degradation ◽  
Soil Microbiome ◽  
Colony Development ◽  
Microbiome Composition ◽  
Spiked Soil

The pollution of arable lands and water with petroleum-derived products is still a valid problem, mainly due the extensive works aimed to improve their production technology to reduce fuel consumption and protect engines. An example of the upgraded fuels is the BP 98 unleaded petrol with Active technology. A pot experiment was carried out in which Eutric Cambisol soil was polluted with petrol to determine its effect on the microbiological and biochemical properties of this soil. Analyses were carried out to determine soil microbiome composition—with the incubation and metagenomic methods, the activity of seven enzymes, and cocksfoot effect on hydrocarbon degradation. The following indices were determined: colony development index (CD); ecophysiological diversity index (EP); index of cocksfoot effect on soil microorganisms and enzymes (IFG); index of petrol effect on soil microorganisms and enzymes (IFP); index of the resistance of microorganisms, enzymes, and cocksfoot to soil pollution with petrol (RS); Shannon–Weaver’s index of bacterial taxa diversity (H); and Shannon–Weaver’s index of hydrocarbon degradation (IDH). The soil pollution with petrol was found to increase population numbers of bacteria and fungi, and Protebacteria phylum abundance as well as to decrease the abundance of Actinobacteria and Acidobacteria phyla. The cultivation of cocksfoot on the petrol-polluted soil had an especially beneficial effect mainly on the bacteria belonging to the Ramlibacter, Pseudoxanthomonas, Mycoplana, and Sphingobium genera. The least susceptible to the soil pollution with petrol and cocksfoot cultivation were the bacteria of the following genera: Kaistobacter, Rhodoplanes, Bacillus, Streptomyces, Paenibacillus, Phenylobacterium, and Terracoccus. Cocksfoot proved effective in the phytoremediation of petrol-polluted soil, as it accelerated hydrocarbon degradation and increased the genetic diversity of bacteria. It additionally enhanced the activities of soil enzymes.

scholarly journals Implications of Soil Pollution with Diesel Oil and BP Petroleum with ACTIVE Technology for Soil Health

2019 ◽  
Vol 16 (14) ◽  
pp. 2474 ◽  
Author(s):  
Agata Borowik ◽  
Jadwiga Wyszkowska ◽  
Mirosław Kucharski ◽  
Jan Kucharski
Keyword(s):  
Soil Pollution ◽  
Soil Enzymes ◽  
Soil Health ◽  
Operational Taxonomic Unit ◽  
Diesel Oil ◽  
Petroleum Products ◽  
Soil Microbiome ◽  
Study Results ◽  
Polycyclic Aromatic ◽  
Properties Of Soil

Grass Elymus elongatus has a potential in phytoremediation and was used in this study in a potted experiment, which was performed to determine the effect of polluting soil (Eutric Cambisol) with diesel oil (DO) and unleaded petroleum (P) on the diversity of soil microorganisms, activity of soil enzymes, physicochemical properties of soil, and on the resistance of Elymus elongatus to DO and P, which altogether allowed evaluating soil health. Both petroleum products were administered in doses of 0 and 7 cm3 kg−1 soil d.m. Vegetation of Elymus elongatus spanned for 105 days. Grasses were harvested three times, i.e., on day 45, 75, and 105 of the experiment. The study results demonstrated a stronger toxic effect of DO than of P on the growth and development of Elymus elongatus. Diesel oil caused greater changes in soil microbiome compared to unleaded petroleum. This hypothesis was additionally confirmed by Shannon and Simpson indices computed based on operational taxonomic unit (OTU) abundance, whose values were the lowest in the DO-polluted soil. Soil pollution with DO reduced the counts of all bacterial taxa and stimulated the activity of soil enzymes, whereas soil pollution with P diminished the diversity of bacteria only at the phylum, class, order, and family levels, but significantly suppressed the enzymatic activity. More polycyclic aromatic hydrocarbons (PAHs) were degraded in the soil polluted with P compared to DO, which may be attributed to the stimulating effect of Elymus elongatus on this process, as it grew better in the soil polluted with P than in that polluted with DO.

scholarly journals The resistance of Lolium perenne L. × hybridum, Poa pratensis, Festuca rubra, F. arundinacea, Phleum pratense and Dactylis glomerata to soil pollution by diesel oil and petroleum

2019 ◽  
Vol 65 (No. 6) ◽  
pp. 307-312 ◽  
Author(s):  
Jadwiga Wyszkowska ◽  
Agata Borowik ◽  
Jan Kucharski
Keyword(s):  
Soil Pollution ◽  
Lolium Perenne ◽  
Poa Pratensis ◽  
Dactylis Glomerata ◽  
Diesel Oil ◽  
Phleum Pratense ◽  
Grass Species ◽  
Festuca Rubra ◽  
Lolium Perenne L ◽  
Phleum Pratense L

Resistance of common European grasses to diesel oil and petroleum pollution is not well-known. Therefore, this study aimed at determining the level of resistance of selected grasses to pollution by diesel and petroleum using the pot experiment. The achieved results were compared with those determined for grasses grown on the non-polluted soil. Soil pollution with the tested products was found to significantly decrease the yield of all grasses, with the decrease being lower upon soil pollution with petroleum than with diesel oil. The most resistant to the pollution with diesel oil and petroleum were Phleum pratense L., Lolium perenne L. and Lolium × hybridum Hausskn. The degradation of particular groups of polycyclic aromatic hydrocarbons (PAHs) depended on their chemical properties, on the type of pollutant and grass species. The greatest degradation was determined in the case of BTEX, C<sub>6</sub>–C<sub>12</sub> benzines as well as 2- and 3-ring hydrocarbons, whereas the lowest in the case of 5-and 6-ring hydrocarbons and C<sub>12</sub>–C<sub>25</sub> oils. The most useful species in the remediation of soils polluted with diesel oil and petroleum turned out to be: Lolium perenne L., Lolium × hybridum Hausskn and Phleum pratense L., whereas the least useful appeared to be: Festuca rubra, Dactylis glomerata L. and Poa pratensis L.

scholarly journals The diversity of fungi consortium isolated from polluted soil for degrading petroleum hydrocarbon

2021 ◽  
Vol 22 (11) ◽  
Author(s):  
RIRYN NOVIANTY ◽  
ANNISA HIDAYAH ◽  
SARYONO SARYONO ◽  
AMIR AWALUDDIN ◽  
NOVA WAHYU PRATIWI ◽  
...  
Keyword(s):  
Crude Oil ◽  
Petroleum Hydrocarbon ◽  
Oil Spills ◽  
Petroleum Industry ◽  
Degradation Process ◽  
Petroleum Products ◽  
Industrial Applications ◽  
Polluted Soil ◽  
Gas Chromatography Mass Spectrometry ◽  
Terrestrial Environments

Abstract. Novianty R, Saryono, Awaluddin A, Pratiwi NW, Hidayah A, Juliantari E. 2021. The diversity of fungi consortium isolated from polluted soil for degrading petroleum hydrocarbon. Biodiversitas 22: 5077-5084. One of the major problems in the petroleum industry nowadays is crude oil spills. Riau Province, Sumatra is one of the largest oil producers in Indonesia. Accidental releases of petroleum products are of particular concern to the environment. The process of drilling and refining petroleum generates a large amount of oil sludge. One of the effective technologies used in the waste degradation process is bioremediation using certain microorganisms. The prime objective of the current research was to evaluate the efficiency of fungi consortiums in crude oil degradation in Bumi Siak Pusako-Pertamina Hulu, Indonesia. There are three potential fungi isolates as petroleum hydrocarbon degradation agents with four consortium variations. The parameter values of Optical Density (OD), pH, and diluted CO2 were measured on 0, 4, 8, and 16 days. To evaluate the fungal biodegradation activity using Gas Chromatography-Mass Spectrometry (GC-MS). The result showed that consortium II (KF II) has the highest potential to degrade petroleum hydrocarbon (50.61%). The visual GC-MS examination confirmed a decrease in the peak area for eight hydrocarbon compounds, indicating the efficiency of the fungi in the oil decomposition and dismantling of hydrocarbons. Our findings may provide new information on native fungal resources from chronically contaminated terrestrial environments, and will be useful for petroleum-contaminated bioremediation and other industrial applications.  

scholarly journals Bioremediation of Nickel and Lead contaminated soil by Vica faba L. plant and AM fungi Glomus mosseae

2015 ◽  
Vol 12 (2) ◽  
pp. 260-265
Author(s):  
Baghdad Science Journal
Keyword(s):  
Root System ◽  
Soil Pollution ◽  
Contaminated Soil ◽  
Glomus Mosseae ◽  
Arbuscular Mycorrhizae ◽  
Am Fungi ◽  
Polluted Soil ◽  
The South ◽  
Shoot System ◽  
North And South

This study is conducted to determine the activity of plant Vica faba and two isolated from arbuscular mycorrhizae fungi (A,B) in bioremediation of soil pollution by Nickel and Lead elements in north and south of Baghdad city. The results showed that the average of soil pollution by Nickel and Lead elements in north of Baghdad was less than the average of soil pollution in the south of Baghdad which recorded 29.0,9.0PPm and 42.0, 25.0PPm respectively. The results show that the isolate A from the polluted soil is more active from isolate B which isolate from unpolluted soil for bioremediation. Vica faba recorded more in accumulate the Lead element in shoot system which was 19.65PPm and in root system was 27.2PPm and for Nickel element 24.65, 27.55PPm in shoot and root respectively.

scholarly journals Effect of soil contamination with diesel oil on yellow lupine yield and macroelements content

2011 ◽  
Vol 50 (No. 5) ◽  
pp. 218-226 ◽  
Author(s):  
M. Wyszkowski ◽  
J. Wyszkowska ◽  
A. Ziółkowska
Keyword(s):  
Soil Contamination ◽  
Soil Pollution ◽  
Diesel Oil ◽  
Quality Characteristics ◽  
Plant Organ ◽  
Organ Type ◽  
Sodium Magnesium ◽  
Oil Concentration ◽  
Yield Quality ◽  
Yellow Lupine

The study has been undertaken to assay the effect of soil contamination with diesel oil on yellow lupine yield and macroelement contents as well as to examine the relationships between the accumulation of macroelements versus the yellow lupine yielding and some yield quality characteristics. The influence of soil pollution with refinery products depended on the type of soil, diesel oil concentration in soil and mineral fertilisation. Increasing contents of soil pollution with diesel oil promoted higher concentrations of phosphorus, sodium, magnesium and calcium in aboveground parts and roots of yellow lupine. More severe contamination was also responsible for depressed yield of yellow lupine aboveground parts and roots as well as accumulation of nitrogen in aboveground parts and potassium in roots of the crop. The addition of nitrogen to soil diversified the yielding and content of macroelements in yellow lupine. The direction these changes took depended on the plant organ, type of soil and degree of diesel oil contamination.

scholarly journals Profiling of Indigenous Biosurfactant-Producing Bacillus Isolates in the Bioremediation of Soil Contaminated by Petroleum Products and Olive Oil

2021 ◽  
Vol 2021 ◽  
pp. 1-15
Author(s):  
Paola Sandra Elenga-Wilson ◽  
Christian Aimé Kayath ◽  
Nicaise Saturnin Mokemiabeka ◽  
Stech Anomene Eckzechel Nzaou ◽  
Etienne Nguimbi ◽  
...  
Keyword(s):  
Olive Oil ◽  
Shigella Flexneri ◽  
Energy Resource ◽  
Diesel Oil ◽  
Petroleum Products ◽  
Antagonistic Effect ◽  
Degrading Bacteria ◽  
Displacement Assay ◽  
Antimicrobial Assay

Petroleum is, up to this date, an inimitable nonrenewable energy resource. Petroleum leakage, which arises during transport, storage, and refining, is the most important contaminant in the environment, as it produces harm to the surrounding ecosystem. Bioremediation is an efficient method used to treat petroleum hydrocarbon-contaminated soil using indigenous microorganisms. The degradation characteristics for a variety of hydrocarbons (hexane, benzene, gasoline, and diesel) were qualitatively and quantitatively investigated using Bacillus isolates. Microbiological and biochemical methods have been used including isolation of oil-degrading bacteria, enzymatic activities, the determination of physicochemical parameters, biosurfactant production and extraction assay, oil displacement assay, antimicrobial assay of the biosurfactants, and bioremediation kinetics. Consequently, of the 60 isolates capable of degrading different hydrocarbons at fast rates, 34 were suspected to be Bacillus isolates capable of growing in 24 h or 48 h on BH medium supplemented with 2% of hexane, benzene, gasoline, diesel, and olive oil, respectively. Among the 34 isolates, 61% (21/34) are capable of producing biosurfactant-like molecules by using gasoline, 70% (24/34) with diesel oil, 85% (29/34) with hexane, and 82% (28/34) with benzene. It was found that biosurfactant-producing isolates are extractable with HCl (100%), ammonium sulphate (95%), chloroform (95%), and ethanol (100%). Biosurfactants showed stability at 20°C, 37°C, 40°C, and 60°C. Biosurfactant secreted by Bacillus strains has shown an antagonistic effect in Escherichia coli, Shigella flexneri 5a M90T, and Bacillus cereus. The selected isolates could therefore be safely used for biodegradation. Substrate biodegradation patterns by individual isolates were found to significantly differ. The study shows that benzene was degraded faster, followed by hexane, gasoline, and finally diesel. The Bacillus consortium used can decrease hydrocarbon content from 195 to 112 (g/kg) in 15 days.

scholarly journals Pig Droppings: A Potential Biostimulatory Candidate for Bioremediation of Diesel Oil-Polluted Soil

2019 ◽  
Vol 4 (6) ◽  
pp. 1933-1942
Author(s):  
Kingsley Tochukwu Ughamba ◽  
Nnabueze Darlington Nnaji ◽  
Kenneth Ejike Ogbonna ◽  
Chukwudi Uzoma Anyanwu
Keyword(s):  
Diesel Oil ◽  
Polluted Soil
Sign in / Sign up

Export Citation Format

Share Document