scholarly journals Methodology for the Implementation of Internal Standard to Laser-Induced Breakdown Spectroscopy Analysis of Soft Tissues

Sensors ◽  
2021 ◽  
Vol 21 (3) ◽  
pp. 900
Author(s):  
Anna Šindelářová ◽  
Pavel Pořízka ◽  
Pavlína Modlitbová ◽  
Lucie Vrlíková ◽  
Kateřina Kiss ◽  
...  

The improving performance of the laser-induced breakdown spectroscopy (LIBS) triggered its utilization in the challenging topic of soft tissue analysis. Alterations of elemental content within soft tissues are commonly assessed and provide further insights in biological research. However, the laser ablation of soft tissues is a complex issue and demands a priori optimization, which is not straightforward in respect to a typical LIBS experiment. Here, we focus on implementing an internal standard into the LIBS elemental analysis of soft tissue samples. We achieve this by extending routine methodology for optimization of soft tissues analysis with a standard spiking method. This step enables a robust optimization procedure of LIBS experimental settings. Considering the implementation of LIBS analysis to the histological routine, we avoid further alterations of the tissue structure. Therefore, we propose a unique methodology of sample preparation, analysis, and subsequent data treatment, which enables the comparison of signal response from heterogenous matrix for different LIBS parameters. Additionally, a brief step-by-step process of optimization to achieve the highest signal-to-noise ratio (SNR) is described. The quality of laser–tissue interaction is investigated on the basis of the zinc signal response, while selected experimental parameters (e.g., defocus, gate delay, laser energy, and ambient atmosphere) are systematically modified.

2014 ◽  
Vol 51 (10) ◽  
pp. 103003 ◽  
Author(s):  
尚丽平 Shang Liping ◽  
李文宏 Li Wenhong ◽  
武志翔 Wu Zhixiang ◽  
王芮雯 Wang Ruiwen ◽  
周强 Zhou Qiang

2021 ◽  
Vol 51 (3) ◽  
Author(s):  
Hussein Salloom ◽  
Tagreed Hamad

In this work, laser-induced breakdown spectroscopy (LIBS) analysis is optimized for direct estimation of elemental composition, thermal conductivity and hardness for Ni-Cr-Nb alloys. These alloys were chosen with a variable elemental content of niobium and chromium. The influence of laser energy and shot numbers on measuring line intensity was investigated. Based on the ratio between two spectral lines, calibration curves were formed to estimate the element concentration and LIBS results were confirmed with related energy-dispersive X-ray spectroscopy (EDS) data. Hardness and thermal conductivity estimation using LIBS were done by measuring the ratio between two spectral lines, plasma excitation temperature and electron density for different samples. Semi-empirical formulas correlated hardness and thermal conductivity with plasma temperature were established.


2018 ◽  
Vol 246 ◽  
pp. 01114
Author(s):  
C. P. Chen ◽  
L. Hei ◽  
S. C. Yu ◽  
W. L. Chen ◽  
M. X. Cai

With the rapid development of spectroscopy technology, laser induced breakdown spectroscopy (LIBS) has become the focus of elemental analysis technology in recent years. With its advantages of non-destructive testing, rapid and multi-element detection, LIBS has been successfully applied to the analysis of geochemical characteristics of soil elements in river basins. The application of LIBS technology in soil elemental analysis of watershed is reviewed in detail. The accuracy of LIBS technology in soil element analysis is studied with the Masha River Basin as the research area. On this basis, the key problems in the process of soil element analysis, such as spectral enhancement methods and quantitative analysis methods, were analyzed. The analysis results show that the accuracy of elemental analysis between LIBS and traditional chemical methods is not much different, but the analysis time of LIBS method is shorter. Methods such as MLR, PLSR, and internal standard methods can improve the fit of the calibration curve and reduce the quantitative analysis error. By changing parameters such as laser energy and delay time, the spectral intensity can be enhanced to reduce the effects of matrix effects. In the process of elemental analysis, the effects of soil physical and chemical parameters should be considered comprehensively, and appropriate quantitative analysis methods should be adopted to improve the accuracy of the analysis results.


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