Raman Spectroscopy-Based Quality Control of “Silicon-On-Insulator” Nanowire Chips for the Detection of Brain Cancer-Associated MicroRNA in Plasma

Application of micro-Raman spectroscopy for the monitoring of quality of nanowire sensor chips fabrication has been demonstrated. Nanowire chips have been fabricated on the basis of «silicon-on-insulator» (SOI) structures (SOI-NW chips). The fabrication of SOI-NW chips was performed by optical litography with gas-phase etching. The so-fabricated SOI-NW chips are intended for highly sensitive detection of brain cancer biomarkers in humans. In our present study, two series of experiments have been conducted. In the first experimental series, detection of a synthetic DNA oligonucleotide (oDNA) analogue of brain cancer-associated microRNA miRNA-363 in purified buffer solution has been performed in order to demonstrate the high detection sensitivity. The second experimental series has been performed in order to reveal miRNA-363 itself in real human plasma samples. To provide detection biospecificity, the SOI-NW chip surface was modified by covalent immobilization of probe oligonucleotides (oDNA probes) complementary to the target biomolecules. Using the SOI-NW sensor chips proposed herein, the concentration detection limit of the target biomolecules at the level of 3.3 × 10−17 M has been demonstrated. Thus, the approach employing the SOI-NW chips proposed herein represents an attractive tool in biomedical practice, aimed at the early revelation of oncological diseases in humans.

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Highly Sensitive Detection of CA 125 Protein with the Use of an n-Type Nanowire Biosensor

Biosensors ◽

10.3390/bios10120210 ◽

2020 ◽

Vol 10 (12) ◽

pp. 210

Author(s):

Kristina A. Malsagova ◽

Tatyana O. Pleshakova ◽

Rafael A. Galiullin ◽

Andrey F. Kozlov ◽

Ivan D. Shumov ◽

...

Keyword(s):

Protein Detection ◽

Covalent Immobilization ◽

Silicon On Insulator ◽

Target Protein ◽

Ca 125 ◽

Minimum Concentration ◽

Immobilized Antibodies ◽

Highly Sensitive ◽

Highly Sensitive Detection ◽

Biospecific Interaction

The detection of CA 125 protein in a solution using a silicon-on-insulator (SOI)-nanowire biosensor with n-type chip has been experimentally demonstrated. The surface of nanowires was modified by covalent immobilization of antibodies against CA 125 in order to provide the biospecificity of the target protein detection. We have demonstrated that the biosensor signal, which results from the biospecific interaction between CA 125 and the covalently immobilized antibodies, increases with the increase in the protein concentration. At that, the minimum concentration, at which the target protein was detectable with the SOI-nanowire biosensor, amounted to 1.5 × 10−16 M.

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Optical Monitoring of the Production Quality of Si-Nanoribbon Chips Intended for the Detection of ASD-Associated Oligonucleotides

Micromachines ◽

10.3390/mi12020147 ◽

2021 ◽

Vol 12 (2) ◽

pp. 147

Author(s):

Kristina A. Malsagova ◽

Tatyana O. Pleshakova ◽

Vladimir P. Popov ◽

Igor N. Kupriyanov ◽

Rafael A. Galiullin ◽

...

Keyword(s):

Production Quality ◽

Autism Spectrum ◽

Covalent Immobilization ◽

Biological Macromolecules ◽

Label Free ◽

Raman Scattering Spectroscopy ◽

Dna Oligonucleotides ◽

Highly Sensitive ◽

Highly Sensitive Detection

Gas-phase etching and optical lithography were employed for the fabrication of a silicon nanoribbon chip (Si-NR chip). The quality of the so-fabricated silicon nanoribbons (Si-NRs) was monitored by optical Raman scattering spectroscopy. It was demonstrated that the structures of the Si-NRs were virtually defect-free, meaning they could be used for highly sensitive detection of biological macromolecules. The Si-NR chips were then used for the highly sensitive nanoelectronics detection of DNA oligonucleotides (oDNAs), which represent synthetic analogs of 106a-5p microRNA (miR-106a-5p), associated with the development of autism spectrum disorders in children. The specificity of the analysis was attained by the sensitization of the Si-NR chip sur-face by covalent immobilization of oDNA probes, whose nucleotide sequence was complementary to the known sequence of miR-106a-5p. The use of the Si-NR chip was demonstrated to al-low for the rapid label-free real-time detection of oDNA at ultra-low (~10−17 M) concentrations.

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Spectroscopic requirements for Raman instrumentation on a planetary lander: potential for the remote detection of biosignatures on Mars

International Journal of Astrobiology ◽

10.1017/s1473550404002022 ◽

2004 ◽

Vol 3 (2) ◽

pp. 165-174 ◽

Cited By ~ 7

Author(s):

S.E. Jorge Villar ◽

H.G.M. Edwards

Keyword(s):

Raman Spectroscopy ◽

Raman Spectra ◽

Spectral Resolution ◽

Detection Sensitivity ◽

Remote Detection ◽

Spectral Identification ◽

Scan Time ◽

Time Parameters ◽

Raman Spectral ◽

Non Destructive

The special characteristics of Raman spectroscopy (relative insensitivity to water, non-destructive detection, sensitivity to bio- and geosignatures, molecular structural composition information, etc.) together with the development of miniaturized Raman spectrometers make the consideration of this technique for future robotic landers on planetary surfaces, particularly Mars, a very interesting option. The development of light and rugged Raman spectrometers limits the possible scope of the instrumentation which has particular importance in the recognition of biomolecular and mineral signatures. In this work, we evaluate the spectral resolution and scan time parameters and the effect that they have on the Raman spectra of extremophilic biomolecules, together with the wavenumber ranges which are critical for the detection of life signals. This is of vital relevance for the design of miniaturized Raman spectrometer systems. From our results, we conclude that for extraterrestrial biological signatures unambiguous Raman spectral identification provided with a minimum of 16 cm−1 spectral resolution is required for the most significant biosignature wavenumber range in the 1700–700 cm−1 region.

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Non-Destructive Assessment of Thin Film Stresses and Crystal Qualify of Silicon on Insulator Materials with Raman Spectroscopy

MRS Proceedings ◽

10.1557/proc-188-53 ◽

1990 ◽

Vol 188 ◽

Cited By ~ 4

Author(s):

Ingrid De Wolf ◽

Jan Vanhellemont ◽

Herman E. Maes

Keyword(s):

Thin Film ◽

Electron Microscopy ◽

Raman Spectroscopy ◽

Cross Section ◽

Silicon Layer ◽

Silicon On Insulator ◽

Micro Raman Spectroscopy ◽

Transmission Electron ◽

Non Destructive ◽

Zone Melt

ABSTRACTMicro Raman spectroscopy (RS) is used to study the crystalline quality and the stresses in the thin superficial silicon layer of Silicon-On-Insulator (SO) materials. Results are presented for SIMOX (Separation by IMplanted OXygen) and ZMR (Zone Melt Recrystallized) substrates. Both as implanted and annealed SIMOX structures are investigated. The results from the as implanted structures are correlated with spectroscopic ellipsometry (SE) and cross-section transmission electron microscopy (TEM) analyses on the same material. Residual stress in ZMR substrates is studied in low- and high temperature gradient regions.

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Feature engineering applied to intraoperative in vivo Raman spectroscopy sheds light on molecular processes in brain cancer: a retrospective study of 65 patients

The Analyst ◽

10.1039/c9an01144g ◽

2019 ◽

Vol 144 (22) ◽

pp. 6517-6532 ◽

Cited By ~ 4

Author(s):

Émile Lemoine ◽

Frédérick Dallaire ◽

Rajeev Yadav ◽

Rajeev Agarwal ◽

Samuel Kadoury ◽

...

Keyword(s):

Raman Spectroscopy ◽

Retrospective Study ◽

Brain Cancer ◽

New Method ◽

Feature Engineering ◽

Molecular Processes ◽

System P ◽

In Vivo Raman Spectroscopy

A new method to improve the statistical interpretability of biological Raman Spectroscopy was applied to spectra acquired in vivo during neurosurgical resection of brain cancer, revealing oncogenic processes captured by the Raman system.

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Detection of thiopurine methyltransferase activity in lysed red blood cells by means of lab-on-a-chip surface enhanced Raman spectroscopy (LOC-SERS)

Analytical and Bioanalytical Chemistry ◽

10.1007/s00216-011-4811-z ◽

2011 ◽

Vol 400 (9) ◽

pp. 2755-2761 ◽

Cited By ~ 49

Author(s):

Anne März ◽

Bettina Mönch ◽

Petra Rösch ◽

Michael Kiehntopf ◽

Thomas Henkel ◽

...

Keyword(s):

Raman Spectroscopy ◽

Red Blood Cells ◽

Blood Cells ◽

Lab On A Chip ◽

Surface Enhanced Raman Spectroscopy ◽

Thiopurine Methyltransferase ◽

Methyltransferase Activity ◽

Chip Surface ◽

Surface Enhanced ◽

Surface Enhanced Raman

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An Experimental Study of Properties of Ultrathin Si Layer with Bonded Si/SiO2 Interface

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.854.3 ◽

2013 ◽

Vol 854 ◽

pp. 3-10

Author(s):

O.V. Naumova ◽

B. Fomin ◽

V.P. Popov ◽

Victor Strelchuk ◽

A. Nikolenko ◽

...

Keyword(s):

Experimental Study ◽

Raman Spectroscopy ◽

Structural Properties ◽

Electron Density ◽

Electron Mobility ◽

Structural Modification ◽

Silicon On Insulator ◽

Float Zone ◽

Bonded Interface ◽

Ultrathin Soi

Properties of Si/buried oxide (BOX) systems with bonded interface in silicon-on-insulator (SOI) wafers were studied in this paper. Results show impact of the starting Si material - Czochralski (Cz) or float-zone (Fz) grown silicon on the electron mobility (μe) and BOX charge behavior in ultrathin SOI layers. In particular, there were found: 1) the μe ~ Ne-0.3 dependencies at the electron density Ne in the range of 4х (1011-1012) cm-2 in accumulation Cz-SOI layers with the μe degradation when Si thickness decreases from 20 to 9 nm, and 2) the ~ Ne-0.6 behavior of mobility with no degradation in Fz-SOI layers. Raman spectroscopy shows the structural modification of Cz-SOI layers. An origin of degradation of the electrical and structural properties for ultrathin SOI layer is discussed.

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