scholarly journals Efficient Sub-1 Minute Analysis of Selected Biomarker Catecholamines by Core-Shell Hydrophilic Interaction Liquid Chromatography (HILIC) with Nanomolar Detection at a Boron-Doped Diamond (BDD) Electrode

Separations ◽  
2021 ◽  
Vol 8 (8) ◽  
pp. 124
Author(s):  
Majidah Alsaeedi ◽  
Huda Alghamdi ◽  
Phyllis Hayes ◽  
Anna Hogan ◽  
Jeremy Glennon
Keyword(s):  
Traumatic Brain Injury ◽  
Liquid Chromatography ◽  
Brain Injury ◽  
Core Shell ◽  
Hydrophilic Interaction ◽  
Boron Doped Diamond ◽  
The Core ◽  
Bdd Electrode ◽  
Boron Doped ◽  
Nanomolar Detection

A rapid, sensitive method for the separation of catecholamine biomarkers (CAs), of importance in traumatic brain injury (TBI) and in Parkinson’s disease (PD), has been successfully developed using hydrophilic interaction liquid chromatography (HILIC). Dopamine (DA), epinephrine (EPI), and norepinephrine (NE) are known to be three to fivefold elevated above normal in traumatic brain injury (TBI) patients. HILIC facilitates the rapid and efficient separation of these polar biomarkers, which can be poorly retained by reversed-phase liquid chromatography (RPLC), while electrochemical detection (ECD) at the boron-doped diamond (BDD) electrode provides enhanced nanomolar detection. Three HILIC columns were compared, namely the superficially porous (core-shell) Z-HILIC column and the Z-cHILIC and Z-HILIC fully porous columns. The core-shell Z-HILIC showed the highest efficiency with a rapid separation within 60 s. The HILIC method utilizing the core-shell Z-HILIC column was initially optimized for the simultaneous analysis of DA, EPI, and NE using UV detection. The advantages of using the BDD electrode over UV detection were explored, and the improved limits of detection (LODs, S/N = 3) measured were 40, 50, and 50 nM for DA, EPI, and NE, respectively. Method validation is reported in terms of the linearity, repeatability, reproducibility, and LODs. Furthermore, the proposed method was successfully applied to the real sample analysis of urinary CAs following phenylboronic acid (PBA) solid phase extraction (SPE) pretreatment.

DICLOFENAC REMOVAL FROM AQUEOUS SOLUTIONS BY ELECTROOXIDATION AT BORON-DOPED DIAMOND (BDD) ELECTRODE

2015 ◽  
Vol 14 (6) ◽  
pp. 1339-1345
Author(s):  
Monica Ihos ◽  
Florica Manea ◽  
Maria Jitaru ◽  
Corneliu Bogatu ◽  
Rodica Pode
Keyword(s):  
Aqueous Solutions ◽  
Boron Doped Diamond ◽  
Bdd Electrode ◽  
Boron Doped

scholarly journals Study of the Electrochemical Deposition of Cu/Sn Alloy Nanoparticles on Boron Doped Diamond Films for Electrocatalytic Nitrate Reduction

2013 ◽  
Vol 1511 ◽  
Author(s):  
Jorge T. Matsushima ◽  
Andrea B. Couto ◽  
Neidenei G. Ferreira ◽  
Mauricio R. Baldan
Keyword(s):  
Electrochemical Deposition ◽  
Sem Analysis ◽  
Homogeneous Distribution ◽  
Alloy Nanoparticles ◽  
Electrocatalytic Reduction ◽  
Boron Doped Diamond ◽  
Buffer Solution ◽  
Bdd Electrode ◽  
Boron Doped ◽  
Dissolution Peak

ABSTRACTThis paper presents the study of the electrochemical deposition of Cu/Sn alloy nanoparticles on Boron Doped Diamond (BDD) films in order to improve their electrocatalytic activity and selectivity for application in nitrate electrochemical reduction. Cyclic voltammetry measurements evidenced the formation of Cu/Sn alloy electrodeposited on BDD electrode. The electrodeposited Cu/Sn can be better visualized by analyzing the dissolution process. By studying the dissolution peak separately, the dissolution peak of the Sn was obtained at a more positive potential, when compared with the dissolution peak of Cu. From the scanning electronic microscopy (SEM) analysis, the homogeneous distribution of the Cu/Sn alloys particles on BDD surface with grain size in nanometric scale was verified. From X-ray diffraction analysis, two Cu/Sn alloy phases (Cu41Sn11 and Cu10Sn3) were identified for the electrodeposits obtained at -0.5V and charge of 0.26 C. The electrocatalytic reduction of nitrate in 0.1 M Britton-Robinson (BR) buffer solution with pH 9 was analyzed. The BDD electrode modified with Cu/Sn alloy nanoparticles proved to potentiate the electrocatalytic reduction of nitrate.

An alternative approach for the preparation of a core–shell bimetallic central metal–organic framework as a hydrophilic interaction liquid chromatography stationary phase

The Analyst ◽  
2020 ◽  
Vol 145 (11) ◽  
pp. 3851-3856 ◽  
Author(s):  
Tiantian Si ◽  
Licheng Wang ◽  
Xiaofeng Lu ◽  
Xiaojing Liang ◽  
Shuai Wang ◽  
...  
Keyword(s):  
Liquid Chromatography ◽  
Stationary Phase ◽  
Metal Organic Framework ◽  
Hydrophilic Interaction Liquid Chromatography ◽  
Core Shell ◽  
Central Metal ◽  
Hydrophilic Interaction ◽  
Alternative Approach ◽  
Metal Organic ◽  
New Type

A new type of core–shell composite material was prepared and applied as a hydrophilic interaction liquid chromatography (HILIC) stationary phase.

scholarly journals Lost Polarization of Aquaporin4 and Dystroglycan in the Core Lesion after Traumatic Brain Injury Suggests Functional Divergence in Evolution

2015 ◽  
Vol 2015 ◽  
pp. 1-10 ◽  
Author(s):  
Hui Liu ◽  
Gou ping Qiu ◽  
Fei Zhuo ◽  
Wei hua Yu ◽  
Shan quan Sun ◽  
...  
Keyword(s):  
Traumatic Brain Injury ◽  
Brain Injury ◽  
Brain Edema ◽  
Early Stage ◽  
Functional Divergence ◽  
Edema Formation ◽  
Aqp4 Expression ◽  
The Core ◽  
Perivascular Area ◽  
Brain Edema Formation

Objective. To understand how aquaporin4 (AQP4) and dystroglycan (DG) polarized distribution change and their roles in brain edema formation after traumatic brain injury (TBI).Methods. Brain water content, Evans blue detection, real-time PCR, western blot, and immunofluorescence were used.Results. At an early stage of TBI, AQP4 and DG maintained vessel-like pattern in perivascular endfeet; M1, M23, and M1/M23 were increased in the core lesion. At a later stage of TBI, DG expression was lost in perivascular area, accompanied with similar but delayed change of AQP4 expression; expression of M1, M23, and DG and the ratio of M1/M2 were increased.Conclusion. At an early stage, AQP4 and DG maintained the polarized distribution. Upregulated M1 and M23 could retard the cytotoxic edema formation. At a later stage AQP4 and DG polarized expression were lost from perivascular endfeet and induced the worst cytotoxic brain edema. The alteration of DG expression could regulate that of AQP4 expression after TBI.

Boron-Doped Diamond Electrode Coupled to Liquid Chromatography: Application to Simultaneous Determination of Benzodiazepines

2010 ◽  
Vol 22 (4) ◽  
pp. 455-462 ◽  
Author(s):  
Isarita Martins ◽  
Larissa de Souza Canaes ◽  
Keity Margareth Doretto ◽  
Susanne Rath
Keyword(s):  
Liquid Chromatography ◽  
Simultaneous Determination ◽  
Boron Doped Diamond ◽  
Diamond Electrode ◽  
Boron Doped ◽  
Boron Doped Diamond Electrode
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