scholarly journals MTF1 Is Essential for the Expression of MT1B, MT1F, MT1G, and MT1H Induced by PHMG, but Not CMIT, in the Human Pulmonary Alveolar Epithelial Cells

Toxics ◽  
2021 ◽  
Vol 9 (9) ◽  
pp. 203
Author(s):  
Sang-Hoon Jeong ◽  
Cherry Kim ◽  
Jaeyoung Kim ◽  
Yoon-Jeong Nam ◽  
Hong Lee ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Epithelial Cells ◽  
Alveolar Epithelial Cells ◽  
Rna Seq ◽  
Protein Expression Level ◽  
Early Growth Response ◽  
Alveolar Epithelial ◽  
Early Growth Response 1 ◽  
Transcription Factor 1 ◽  
Essential Transcription Factor

The inhalation of humidifier disinfectants (HDs) is linked to HD-associated lung injury (HDLI). Polyhexamethylene guanidine (PHMG) is significantly involved in HDLI, but the correlation between chloromethylisothiazolinone (CMIT) and HDLI remains ambiguous. Additionally, the differences in the molecular responses to PHMG and CMIT are poorly understood. In this study, RNA sequencing (RNA-seq) data showed that the expression levels of metallothionein-1 (MT1) isoforms, including MT1B, MT1E, MT1F, MT1G, MT1H, MT1M, and MT1X, were increased in human pulmonary alveolar epithelial cells (HPAEpiCs) that were treated with PHMG but not in those treated with CMIT. Moreover, upregulation of MT1B, MT1F, MT1G, and MT1H was observed only in PHMG-treated HPAEpiCs. The protein expression level of metal regulatory transcription factor 1 (MTF1), which binds to the promoters of MT1 isoforms, was increased in PHMG-treated HPAEpiCs but not in CMIT-treated HPAEpiCs. However, the expression of early growth response 1 (EGR1) and nuclear receptor superfamily 3, group C, member 1 (NR3C1), other transcriptional regulators involved in MT1 isomers, were increased regardless of treatment with PHMG or CMIT. These results suggest that MTF1 is an essential transcription factor for the induction of MT1B, MT1F, MT1G, and MT1H by PHMG but not by CMIT.

Induction of the early growth response-1 transcription factor in gastric epithelial cells by Helicobacter pylori

2000 ◽  
Vol 118 (4) ◽  
pp. A737
Author(s):  
Mohamed M. Abdel-Iatif ◽  
Henry John Windle ◽  
Yeng Shiong Ang ◽  
Deidre Ni Eidhin ◽  
Kamal Sabra ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Transcription Factor ◽  
Epithelial Cells ◽  
Growth Response ◽  
Early Growth ◽  
Gastric Epithelial Cells ◽  
Early Growth Response ◽  
Early Growth Response 1

scholarly journals Expression of Transcription Factor GATA-6 in Alveolar Epithelial Cells Is Linked to Neonatal Lung Disease

Neonatology ◽  
2011 ◽  
Vol 99 (3) ◽  
pp. 231-240 ◽  
Author(s):  
Riika Vähätalo ◽  
Tiina M. Asikainen ◽  
Riitta Karikoski ◽  
Vuokko L. Kinnula ◽  
Carl W. White ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Epithelial Cells ◽  
Lung Disease ◽  
Alveolar Epithelial Cells ◽  
Alveolar Epithelial ◽  
Neonatal Lung

scholarly journals CD34 Expression in the Stromal Cells of Alveolar Adenoma

2012 ◽  
Vol 2012 ◽  
pp. 1-4 ◽  
Author(s):  
Nicolina De Rosa ◽  
Alfonso Maiorino ◽  
Ilaria De Rosa ◽  
Carlo Curcio ◽  
Carmine Sellitto ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Stromal Cells ◽  
Alveolar Epithelial Cells ◽  
Alveolar Epithelial ◽  
Thyroid Transcription Factor 1 ◽  
Thyroid Transcription Factor ◽  
Rare Benign Tumor ◽  
Transcription Factor 1 ◽  
Cd34 Positivity

The alveolar adenoma of the lung is a rare benign tumor characterized by a proliferation of both the alveolar epithelial cells and the mesenchymal septal cells. Immunohistochemically, the epithelial cells stain for cytokeratin (CK) AE1AE3, CK7, thyroid transcription factor 1 (TTF1), and surfactant apoprotein confirming the derivation by the type 2 pneumocytes. The stromal cells are negative for these markers but they show focally smooth muscle and muscle-specific actin positivity. We describe two cases that showed immunohistochemically a CD34 positivity of the mesenchymal septal cells. This aspect has been previously described in a two cases report, but not emphasized by the authors as a distinctive feature of the lesion. We consider this CD34 positivity as a marker of immaturity or stemness of the lesional septal spindle cells, that could be responsible of the different phenotypic and morphological profile of the interstitial cells, that could be, therefore, considered neoplastic and not reactive.

scholarly journals Forkhead Transcription Factor FOXO3a Protects Alveolar Epithelial Cells from Oxidative Stress

2011 ◽  
Vol 25 (S1) ◽  
Author(s):  
Jamileh Ahmed ◽  
KiranKumar Battula ◽  
Ruan R Cox ◽  
Salman Aljubran ◽  
Narasaiah Kolliputi
Keyword(s):  
Oxidative Stress ◽  
Transcription Factor ◽  
Epithelial Cells ◽  
Alveolar Epithelial Cells ◽  
Forkhead Transcription Factor ◽  
Alveolar Epithelial

scholarly journals Time-resolved dual RNA-Seq reveals extensive rewiring of lung epithelial and pneumococcal transcriptomes during early infection

2016 ◽  
Author(s):  
Rieza Aprianto ◽  
Jelle Slager ◽  
Siger Holsappel ◽  
Jan-Willem Veening
Keyword(s):  
Epithelial Cells ◽  
Pneumococcal Infection ◽  
Alveolar Epithelial Cells ◽  
Lung Epithelial Cells ◽  
Infection Model ◽  
Rna Seq ◽  
Time Resolved ◽  
Alveolar Epithelial ◽  
Lung Epithelial ◽  
Host Microbe Interactions

AbstractStreptococcus pneumoniae(pneumococcus) is the main etiological agent of pneumonia. Pneumococcal pneumonia is initiated by bacterial adherence to lung epithelial cells. Infection to the epithelium is a disruptive interspecies interaction involving numerous transcription-mediated processes. Revealing transcriptional changes may provide valuable insights into pneumococcal disease. Dual RNA-Seq allows simultaneous monitoring of the transcriptomes of both host and pathogen. Here, we developed a time-resolved infection model of human lung alveolar epithelial cells byS. pneumoniaeand assessed transcriptome changes by dual RNA-Seq. Our data provide new insights into host-microbe interactions and show that the epithelial glutathione-detoxification pathway is activated by bacterial presence. We observed that adherent pneumococci, not free-floating bacteria, access host-associated carbohydrates and repress innate immune responses. In conclusion, we provide a dynamic dual-transcriptomics overview of early pneumococcal infection with easy online access (http://dualrnaseq.molgenrug.nl). Further database exploration may expand our understanding of epithelial-pneumococcal interaction, leading to novel antimicrobial strategies.Graphical Abstract

Alveolar epithelial cells type II show a high sensitivity to cigarette smoke extract

Pneumologie ◽  
2014 ◽  
Vol 68 (06) ◽  
Author(s):  
S Seehase ◽  
B Baron-Luehr ◽  
C Kugler ◽  
E Vollmer ◽  
T Goldmann
Keyword(s):  
Epithelial Cells ◽  
Cigarette Smoke ◽  
High Sensitivity ◽  
Cigarette Smoke Extract ◽  
Alveolar Epithelial Cells ◽  
Type Ii ◽  
Alveolar Epithelial
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