scholarly journals The Inactivation by Curcumin-Mediated Photosensitization of Botrytis cinerea Spores Isolated from Strawberry Fruits

Toxins ◽  
2021 ◽  
Vol 13 (3) ◽  
pp. 196
Author(s):  
Li Huang ◽  
Ken W. L. Yong ◽  
W. Chrishanthi Fernando ◽  
Matheus Carpinelli de Jesus ◽  
James J. De Voss ◽  
...  
Keyword(s):  
Botrytis Cinerea ◽  
Food Industry ◽  
Fungal Spores ◽  
Grey Mould ◽  
Phytopathogenic Fungi ◽  
Natural Food ◽  
Percentage Reduction ◽  
Spore Viability ◽  
Food Dye ◽  
The Common

Photosensitization is a novel environmentally friendly technology with promising applications in the food industry to extend food shelf life. In this study, the natural food dye curcumin, when combined with visible light (430 nm), was shown to be an effective photosensitizer against the common phytopathogenic fungi Botrytis cinerea (the cause of grey mould). Production of the associated phytotoxic metabolites botrydial and dihydrobotrydial was measured by our newly developed and validated HRAM UPLC-MS/MS method, and was also shown to be reduced by this treatment. With a light dose of 120 J/cm2, the reduction in spore viability was directly proportional to curcumin concentrations, and the overall concentration of both botrydial and dihydrobotrydial also decreased with increasing curcumin concentration above 200 µM. With curcumin concentrations above 600 µM, the percentage reduction in fungal spores was close to 100%. When the dye concentration was increased to 800 µM, the spores were completely inactive and neither botrydial nor dihydrobotrydial could be detected. These results suggest that curcumin-mediated photosensitization is a potentially effective method to control B. cinerea spoilage, and also to reduce the formation of these phytotoxic botryane secondary metabolites.

scholarly journals Characterization of the Role of a Non-GPCR Membrane-Bound CFEM Protein in the Pathogenicity and Germination of Botrytis cinerea

2020 ◽  
Vol 8 (7) ◽  
pp. 1043
Author(s):  
Gulab Chand Arya ◽  
Dhruv Aditya Srivastava ◽  
Eswari P. J. Pandaranayaka ◽  
Ekaterina Manasherova ◽  
Dov Bernard Prusky ◽  
...  
Keyword(s):  
Botrytis Cinerea ◽  
Pathogenic Fungi ◽  
Phytopathogenic Fungi ◽  
Fungal Virulence ◽  
Wide Range ◽  
Membrane Bound ◽  
The Common ◽  
G Protein Coupled

The necrotrophic fungus Botrytis cinerea, is considered a major cause of postharvest losses in a wide range of crops. The common fungal extracellular membrane protein (CFEM), containing a conserved eight-cysteine pattern, was found exclusively in fungi. Previous studies in phytopathogenic fungi have demonstrated the role of membrane-bound and secreted CFEM-containing proteins in different aspects of fungal virulence. However, non-G protein-coupled receptor (non-GPCR) membrane CFEM proteins have not been studied yet in phytopathogenic fungi. In the present study, we have identified a non-GPCR membrane-bound CFEM-containing protein, Bcin07g03260, in the B. cinerea genome, and generated deletion mutants, ΔCFEM-Bcin07g03260, to study its potential role in physiology and virulence. Three independent ΔCFEM-Bcin07g03260 mutants showed significantly reduced progression of a necrotic lesion on tomato (Solanum lycopersicum) leaves. Further analysis of the mutants revealed significant reduction (approximately 20–30%) in conidial germination and consequent germ tube elongation compared with the WT. Our data complements a previous study of secreted ΔCFEM1 mutants of B. cinerea that showed reduced progression of necrotic lesions on leaves, without effect on germination. Considering various functions identified for CFEM proteins in fungal virulence, our work illustrates a potential new role for a non-GPCR membrane CFEM in pathogenic fungi to control virulence in the fungus B. cinerea.

scholarly journals Investigation of Botrytis Cinerea Risk Forecasting Model of Strawberry in Lithuania

2013 ◽  
Vol 67 (2) ◽  
pp. 195-198 ◽  
Author(s):  
Neringa Rasiukevičiūtė ◽  
Alma Valiuškaitė ◽  
Elena Survilienė-Radzevičė ◽  
Skaidrė Supronienė
Keyword(s):  
Disease Management ◽  
Botrytis Cinerea ◽  
Air Temperature ◽  
Grey Mould ◽  
Management Systems ◽  
Forecasting Model ◽  
Leaf Wetness ◽  
Risk Of Infection ◽  
Risk Forecasting ◽  
Risk Probability

Grey mould, caused by Botrytis cinerea Pers.:Fr. is one of the most important strawberry diseases in Lithuania, like in other countries, where strawberries are grown. The efficiency of different disease management systems were analyzed at the Institute of Horticulture in 2010-2011. The B. cinerea risk probability at various regions of Lithuania was analyzed according to iMETOS ®sm grey mould risk forecasting model. Strawberry grey mould risk forecasting model indicates the risk of infection periods on the basis of the interaction between air temperature and leaf wetness duration. The model calculates how favourable is the period for the risk of infection. In periods where the risk is consistent (more than three days), higher than 60 points, a spray against grey mould should be applied. iMETOS®sm grey mould risk forecasting model gives the opportunity to optimize the usage of fungicides and reduce the number of applications and allows more efficient, ecologically and economically accepted control of strawberries grey mould.

scholarly journals Host‐induced gene silencing of BcTOR in Botrytis cinerea enhances plant resistance to grey mould

2019 ◽  
Vol 20 (12) ◽  
pp. 1722-1739 ◽  
Author(s):  
Fangjie Xiong ◽  
Mei Liu ◽  
Fengping Zhuo ◽  
Huan Yin ◽  
Kexuan Deng ◽  
...  
Keyword(s):  
Gene Silencing ◽  
Botrytis Cinerea ◽  
Plant Resistance ◽  
Grey Mould

scholarly journals Comparison Strawberry Field Production and Occurrence of Grey Mould at Strawberry Growing on System with Addition of Apple Tree Woody Chips

2017 ◽  
Vol 65 (4) ◽  
pp. 1253-1264
Author(s):  
David Novotný ◽  
Jana Brožová ◽  
Pavla Růžičková ◽  
Josef Sus ◽  
Martin Koudela ◽  
...  
Keyword(s):  
Botrytis Cinerea ◽  
Apple Tree ◽  
Grey Mould ◽  
Fruit Weight ◽  
Wood Chips ◽  
Average Weight ◽  
Tree Wood ◽  
Significant Difference ◽  
And Control ◽  
Field Production

In the years 2014 to 2016 the influence of apple tree wood chips and composted apple tree wood chips in which oyster mushroom grew and yielded which were supplemented into soil on strawberry field production and occurrence of grey mould at strawberry (cv. ‘Sonata’) was evaluated. Average weight and number of fruits per plant, average fruit weight, average weight and number of fruits affected by Botrytis cinerea per plant, percentage of weight and number of fruits affected by Botrytis cinerea was calculated. There were recorded differences concerning strawberry yield among the investigated variants of plots, but mainly there were not statistically significant. In 2015 and 2016 the highest average weight of yield and the highest number of fruits was reached at plants cultivated on plots with apple tree wood chips. Average weight of yield on plots with apple tree wood chips was 663.72 g and 822.41 g in 2015 and 2016, respectively. Number of fruits per plant on plots with apple tree wood chips was 67.88 pieces and 65.29 pieces in 2015 and 2016, respectively. Occurrence of grey mould caused by Botrytis cinerea on all variants of plots was similar and small differences was found out only but statistically significant difference was among the years. Grey mould was the most frequently found out in the year 2016. B. cinerea affected 48.27, 56.89 and 55.94 grams of fruits per plant from plots with composted apple tree wood chips, plots with apple tree wood chips and control plots in 2016, respectively.

scholarly journals Micromycetes on Pisum sativum var. arvense

2014 ◽  
Vol 32 (1) ◽  
pp. 31-39
Author(s):  
Joanna Marcinkowska
Keyword(s):  
Pisum Sativum ◽  
Botrytis Cinerea ◽  
Sclerotinia Sclerotiorum ◽  
Alternaria Alternata ◽  
Agar Medium ◽  
Mycosphaerella Pinodes ◽  
Stemphylium Botryosum ◽  
Number Of Species ◽  
Fusarium Poae ◽  
The Common

Seeds of 11 Austrian winter pea genotypes, harvested at Radzików (CEP) in 1993 and 1994, were evaluated for fungi occurrence on Coon's agar medium in Petri plates. Number of species isolated depended on the genotype and year of collection. <i>Alternaria alternata, Stemphylium botryosum</i> were found on all the tested samples and <i>Phoma pinodella</i> and <i>Fusarium poae</i> were also common while <i>Botrytis cinerea, Sclerotinia sclerotiorum</i> and <i>Mycosphaerella pinodes</i> appeared to be common only in 1993. Three species occurred only once. The mycoflora was richer in 1993. The common seed inhabitants usually transmitted higher percentage of fungi than species occuring more seldom.

Sign in / Sign up

Export Citation Format

Share Document