scholarly journals Serological Survey of Lyssaviruses in Polish Bats in the Frame of Passive Rabies Surveillance Using an Enzyme-Linked Immunosorbent Assay

Viruses ◽  
2020 ◽  
Vol 12 (3) ◽  
pp. 271
Author(s):  
Anna Orłowska ◽  
Marcin Smreczak ◽  
Conrad Martin Freuling ◽  
Thomas Müller ◽  
Paweł Trębas ◽  
...  
Keyword(s):  
North American ◽  
Eptesicus Fuscus ◽  
Enzyme Linked Immunosorbent Assay ◽  
Serological Survey ◽  
Specific Antibodies ◽  
Pipistrellus Pipistrellus ◽  
Western Asia ◽  
Myotis Daubentonii ◽  
Eptesicus Serotinus ◽  
Nyctalus Noctula

Background: Bats are known to host a number of nonpathogenic viruses, as well as highly pathogenic viruses causing fatal diseases like rabies. Serological surveys as part of active and passive bat rabies surveillance mainly use seroneutralization assays, demonstrating the presence of lyssavirus-specific antibodies in a variety of European bats, particularly against European bat lyssaviruses type 1 (EBLV-1). Here, we present the first serological survey in European bats of this kind during which European bats from Poland collected in the frame of passive rabies surveillance between 2012 and 2018, as well as Serotine bats (Eptesicus serotinus) and North American Big Brown bats (Eptesicus fuscus) from previous experimental studies, were tested using a commercial ELISA kit for the detection of anti-lyssavirus antibodies. Results: Lyssavirus-specific antibodies were detected in 35 (30.4%) out of 115 Polish bats of both sexes, representing nine out of 13 identified bat species endemic mainly to Central Southern Europe and Western Asia, i.e., Eptesicus serotinus, Nyctalus noctula, Myotis daubentonii, Plecotus auritus, Vespertillo murinus,Pipistrellus pipistrellus, Pipistrellus pipilstrellus/Pipistrellus pygmaeus, Myotis brandtii, and Barbastella barbastellus. Seroprevalence was highest in bat species of Nyctalus noctula, Eptesicus serotinus, Plecotus auritus, and Myotis daubentonii. More than 60% of the ELISA seropositive bats originated from the voivodeships of Silesia, Lower-Silesian, Warmian-Mazurian, and Mazowian. Rabies-specific antibodies were also found in Eptesicus fuscus bats from North America. Conclusions: The study demonstrates the principal application of the BioPro Rabies ELISA Ab Kit for the detection of anti-lyssavirus specific antibodies in body fluids and serum samples of bats. However, results may only be reliable for North American bats, whereas interpretation of results for European bats per se is difficult because proper validation of the test is hampered by the protected status of these species.

scholarly journals Bats of the city of Banská Bystrica, Central Slovakia (Chiroptera)

2016 ◽  
Vol 47 (1) ◽  
pp. 5-15
Author(s):  
Peter Bačkor
Keyword(s):  
Field Survey ◽  
Common Species ◽  
Urban Habitat ◽  
Pipistrellus Pipistrellus ◽  
Concrete Blocks ◽  
Myotis Daubentonii ◽  
Maternity Roost ◽  
The City ◽  
Eptesicus Serotinus ◽  
Nyctalus Noctula

Abstract Based on published findings (1900–2010), unpublished data from museum specimens (1965–1999) and data from recent field survey (2006–2013), the presence of altogether 15 bats species was recorded from the urban area of Banská Bystrica in Central Slovakia. Nyctalus noctula, Vespertilio murinus, Pipistrellus pipistrellus, Eptesicus serotinus and Myotis daubentonii were found as common species in the area. Nyctalus noctula was a predominant species (D=67.4), followed by Vespertilio murinus (D=15.2) and Pipistrellus pipistrellus (D=9.8). The systems of concrete blocks of flats (attic ventilation holes, dilatation fissures, various systems of plumbing accessories, damages of buildings, etc.) represent the most important features of the artificial urban habitat for bats. Most frequently, the hibernation aggregations found in the buildings were formed by Nyctalus noctula, Vespertilio murinus and Pipistrellus pipistrellus, while the occurrence of Myotis myotis, M. mystacinus and M. emargiantus was only occasional. No maternity roost of bats was recorded in the city of Banská Bystrica.

scholarly journals One night in San Marino

2020 ◽  
Vol 189 (1) ◽  
pp. 163-166
Author(s):  
Petr Benda ◽  
Marcel Uhrin
Keyword(s):  
Acoustic Survey ◽  
Foraging Activity ◽  
Pipistrellus Pipistrellus ◽  
First Time ◽  
Eptesicus Serotinus ◽  
Nyctalus Noctula

During several hours of acoustic survey of bats in Borgo Maggiore, San Marino, on 30 August 2020, foraging activity of at least six bat species was recorded. Five of them (Eptesicus serotinus, Hypsugo savii, Pipistrellus pipistrellus, P. kuhlii, and Tadarida teniotis) are common and widespread across the Apennine Peninsula and are already known members of the bat fauna of San Marino. The sixth species, Nyctalus noctula, is here reported from this country for the first time and the bat fauna of San Marino increases to 18 species.

scholarly journals Hibernation of bats in post-Soviet military objects in Poland

2020 ◽  
Vol 63 (1) ◽  
pp. 53-61
Author(s):  
Grzegorz WOJTASZYN ◽  
◽  
Wojciech STEPHAN ◽  
Tomasz RUTKOWSKI ◽  
Jarosław JAROS ◽  
...  
Keyword(s):  
Endangered Species ◽  
Habitats Directive ◽  
Nature Protection ◽  
Northern Group ◽  
Pipistrellus Pipistrellus ◽  
Myotis Myotis ◽  
Eptesicus Serotinus ◽  
Nyctalus Noctula

In the years 2004-2018, data on bats wintering in 9 sites of the Northern Group of the Soviet Army (PGWAR) stationed in the years 1945-1993 in Poland were obtained. A total of at least 11 bat species were found: greater mouse-eared bat Myotis myotis, Natterer’s bat M. nattereri, Daubenton’s bat M. daubentonii, Bechstein’s bat M. bechsteinii, whiskered/Brandt’s/Alcathoe bats M. mystacinus/brandtii/alcathoe, brown long-eared bat Plecotus auritus, grey long-eared bat P. austriacus, western barbastelle Barbastella barbastellus, serotine bat Eptesicus serotinus, common noctule Nyctalus noctula, common pipistrelle Pipistrellus pipistrellus and bats determined to the genus Pipistrellus. From 6 to 449 individuals hibernated in the facilities. Noteworthy is the presence of three species from Annex II of the Habitats Directive of EU. In most of the facilities, M. myotis and B. barbastellus were found, and M. bechsteinii in Wilkocin only. Wintering sites were used by numerous bats and also by endangered species therefore those buildings should be advisable to be covered with forms of nature protection.

scholarly journals On the bat fauna of the Dnipro-Orilskiy Nature Reserve

2020 ◽  
pp. 55-60
Author(s):  
Galina Zadorozhna ◽  
Dmytro Ganzha
Keyword(s):  
Nature Reserve ◽  
Natural Habitats ◽  
Interesting Fact ◽  
Pipistrellus Pipistrellus ◽  
Wintering Range ◽  
Eptesicus Serotinus ◽  
Nyctalus Noctula ◽  
Visual Observations

Data on visual observations of bats in the territory of the Dnipro-Orilskiy Nature Reserve during 2013–2020 are given. The photos that were taken at the same time are presented. From the existing checklist of bats occurring in the reserve, four species were recorded during the mentioned period: Eptesicus serotinus (2 specimens), Pipistrellus pipistrellus (1 specimen), Nyctalus noctula (about 40 specimens), and Pipistrellus kuhlii (about 30 specimens). The most interesting fact is the finding of Nyctalus noctula in November, which is due to the tendency to expand its wintering range. The occurrence of Pipistrellus kuhlii in natural habitats was also revealed, which is uncommon for this species.

scholarly journals PEDV Infection Generates Conformation-Specific Antibodies That Can Be Effectively Detected by a Cell-Based ELISA

Viruses ◽  
2021 ◽  
Vol 13 (2) ◽  
pp. 303
Author(s):  
Wei-Ting Hsu ◽  
Chia-Yu Chang ◽  
Chih-Hsuan Tsai ◽  
Sung-Chan Wei ◽  
Huei-Ru Lo ◽  
...  
Keyword(s):  
Recombinant Baculovirus ◽  
Immunocytochemical Staining ◽  
Enzyme Linked Immunosorbent Assay ◽  
Serum Samples ◽  
Perfect Agreement ◽  
Specific Antibodies ◽  
Diarrhea Virus ◽  
Serological Studies ◽  
A Cell ◽  
Infected Insect

Porcine epidemic diarrhea virus (PEDV) is a coronavirus that causes serious and highly contagious enteric disease in swine worldwide. In this study, we constructed a recombinant baculovirus (S-Bac) expressing full-length spike protein of the virulent epidemic genotype 2b (G2b) PEDV strain for serological studies of infected pigs. We found that most spike-specific antibodies produced upon PEDV infection in pigs are conformation-specific and they could be detected on S-Bac-infected insect cells by immunofluorescent assay, but they were insensitive to Western blot analysis, the typical method for antiserum analysis. These results indicated that spike conformation is crucial for serum recognition. Since it is difficult to purify trimeric spike membrane protein for conventional enzyme-linked immunosorbent assay (ELISA), we used S-Bac to generate a novel cell-based ELISA for convenient PEDV detection. We analyzed 100 pig serum samples, and our cell-based ELISA exhibited a sensitivity of 100%, a specificity of 97%, and almost perfect agreement [Cohen’s kappa coefficient value (κ) = 0.98] with immunocytochemical staining results. Our cell-based ELISA rapidly presented antigen for proper detection of conformation-specific antibodies, making PEDV detection more convenient, and it will be useful for detecting many viral diseases in the future.

scholarly journals High-throughput quantitation of SARS-CoV-2 antibodies in a single-dilution homogeneous assay

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Markus H. Kainulainen ◽  
Eric Bergeron ◽  
Payel Chatterjee ◽  
Asheley P. Chapman ◽  
Joo Lee ◽  
...  
Keyword(s):  
Enzyme Linked Immunosorbent Assay ◽  
Specific Antibodies ◽  
Negative Results ◽  
A Value ◽  
Elisa Testing ◽  
The World ◽  
Antibody Titers ◽  
Qualitative Assay ◽  
Homogeneous Assay ◽  
Asymptomatic Infections

AbstractSARS-CoV-2 emerged in late 2019 and has since spread around the world, causing a pandemic of the respiratory disease COVID-19. Detecting antibodies against the virus is an essential tool for tracking infections and developing vaccines. Such tests, primarily utilizing the enzyme-linked immunosorbent assay (ELISA) principle, can be either qualitative (reporting positive/negative results) or quantitative (reporting a value representing the quantity of specific antibodies). Quantitation is vital for determining stability or decline of antibody titers in convalescence, efficacy of different vaccination regimens, and detection of asymptomatic infections. Quantitation typically requires two-step ELISA testing, in which samples are first screened in a qualitative assay and positive samples are subsequently analyzed as a dilution series. To overcome the throughput limitations of this approach, we developed a simpler and faster system that is highly automatable and achieves quantitation in a single-dilution screening format with sensitivity and specificity comparable to those of ELISA.

scholarly journals Standardization of dot-ELISA for the serological diagnosis of toxocariasis and comparision of the assay with ELISA

1992 ◽  
Vol 34 (1) ◽  
pp. 55-60 ◽  
Author(s):  
Eide Dias Camargo ◽  
Paulo Mutuko Nakamura ◽  
Adelaide José Vaz ◽  
Marcos Vinícius da Silva ◽  
Pedro Paulo Chieffi ◽  
...  
Keyword(s):  
Low Cost ◽  
Clinical Signs ◽  
Toxocara Canis ◽  
Enzyme Linked Immunosorbent Assay ◽  
Serum Samples ◽  
Specific Antibodies ◽  
Normal Individuals ◽  
Before And After ◽  
Dot Elisa ◽  
Stability Time

The dot-enzyme-linked immunosorbent assay (dot-ELISA) was standardized using somatic (S) and excretory-secretory (ES) antigens of Toxocara-canis for the detection of specific antibodies in 22 serum samples from children aged 1 to 15 years, with clinical signs of toxocariasis. Fourteen serum samples from apparently normal individuals and 28 sera from patients with other pathologies were used as controls. All samples were used before and after absorption with Ascaris suum extract. When the results were evaluated in comparison with ELISA, the two tests were found to have similar sensitivity, but dot-ELISA was found to be more specific in the presence of the two antigens studied. Dot-ELISA proved to be effective for the diagnosis of human toxocariasis, presenting advantages in terms of yield, stability, time and ease of execution and low cost.

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