Whole Genome Characterization and Genetic Evolution Analysis of a New Ostrich Parvovirus

Ostrich diseases characterized by paralysis have been breaking out in broad areas of China since 2015, causing major damage to the ostrich breeding industry in China. This report describes a parvovirus detected in ostriches from four different regions. The entire genomes of four parvovirus strains were sequenced following amplification by PCR, and we conducted comprehensive analysis of the ostrich parvovirus genome. Results showed that the length genomes of the parvovirus contained two open reading frames. Ostrich parvovirus (OsPV) is a branch of goose parvovirus (GPV). Genetic distance analysis revealed a close relationship between the parvovirus and goose parvovirus strains from China, with the closest being the 2016 goose parvovirus RC16 strain from Chongqing. This is the first report of a parvovirus in ostriches. However, whether OsPV is the pathogen of ostrich paralysis remains uncertain. This study contributes new information about the evolution and epidemiology of parvovirus in China, which provides a new way for the study of paralysis in ostriches.

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Identifikasi Tumbuhan Paku Air (Azolla sp.) Secara Morfologi dan Molekuler dengan Menggunakan Gen rbcL (Identification of Water Ferns (Azolla sp.) Based on Morphologycal Traits and Molecular Marker Using rbcL Gene)

JURNAL BIOS LOGOS ◽

10.35799/jbl.8.2.2018.21445 ◽

2018 ◽

Vol 8 (2) ◽

pp. 38

Author(s):

Wianita Mantang ◽

Feky R. Mantiri ◽

Beivy J. Kolondam

Keyword(s):

Genetic Distance ◽

Morphological Characters ◽

Morphological Identification ◽

Rbcl Gene ◽

Distance Analysis ◽

Central Java ◽

North Sulawesi ◽

Close Relationship ◽

Water Ferns ◽

Genetic Distance Analysis

Abstrak Azolla merupakan salah satu tumbuhan paku air yang memiliki banyak manfaat, namun belum banyak dikenal dan sering dianggap sebagai tumbuhan gulma. Pengidentifikasian akan keberadaan jenis-jenis tumbuhan paku air (Azolla sp.) penting untuk dilakukan guna mendukung upaya pengembangan, pembudidayaan dan eksplorasi tumbuhan Azolla sp. Penelitian ini bertujuan untuk mengidentifikasi spesies tumbuhan paku air (Azolla sp.) secara morfologi dan molekuler dengan menggunakan gen rbcL. Hasil penelitian menunjukkan bahwa berdasarkan karakter morfologi sampel tumbuhan Azolla sp. asal Tondano Sulawesi Utara menunjukkan kemiripan dengan spesies A. pinnata dan Azolla asal Magelang Jawa Tengah menunjukkan kemiripan dengan spesies A. microphylla. Identifikasi menggunakan sekuens gen rbcL menunjukkan bahwa sekuens sampel tumbuhan Azolla asal Tondano (WM1) memiliki tingkat kemiripan 100% dengan A. pinnata dan Azolla asal Magelang (WM2) memiliki tingkat kemiripan 100% dengan A. microphylla yang terdapat dalam GenBank. Analisis jarak genetik menunjukkan kedua sampel WM1 dan WM2 memiliki hubungan kekerabatan yang cukup dekat dengan nilai jarak genetik 0,060.Kata kunci: Azolla sp., identifikasi morfologi, identifikasi molekuler, gen rbcL Abstract Azolla is one of the water ferns that has many benefits, but it is not yet widely known and is often regarded as a weed plant. Identification of water ferns (Azolla sp.) is important to be carried out to support the development, cultivation, and exploration of Azolla sp. This study aimed to identify species of aquatic plants (Azolla sp.) morphologically and molecularly using the gene rbcL. The results demonstrated that based on the morphological characters, the Azolla sp. from Tondano, North Sulawesi, showed similarity with species A. pinnata and Azolla from Magelang, Central Java, showed similarity to species A. microphylla. Identification using rbcL gene sequences showed that the sample sequence of plants Azolla from Tondano (WM1) had a 100% similarity level with A. pinnata and Azolla from Magelang (WM2) had a 100% similarity level with A. microphylla available in GenBank. Genetic distance analysis showed that both WM1 and WM2 samples had a close relationship with the genetic distance value of 0.060.Key words: Azolla sp., morphological identification, molecular identification, rbcL gene

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Identification of Beak and Feather Disease Virus in an Unusual Novel Host ( Merops ornatus ) Using Nested PCR

Genome Announcements ◽

10.1128/genomea.01376-16 ◽

2016 ◽

Vol 4 (6) ◽

Author(s):

Subir Sarker ◽

Shubhagata Das ◽

Karla Helbig ◽

Shane R. Raidal

Keyword(s):

Disease Virus ◽

Complete Genome Sequence ◽

Host Species ◽

Nested Pcr ◽

Complete Genome ◽

Open Reading Frames ◽

Genome Characterization ◽

Novel Host ◽

Feather Disease Virus ◽

Reading Frames

The complete genome sequence of beak and feather disease virus (BFDV) was discovered from a rainbow bee-eater ( Merops ornatus ), a species of Coraciiformes . The genome consisted of 1,996 bp encoding two major bidirectional transcribed open reading frames. This is the first evidence of BFDV infection and complete genome characterization for this novel host species.

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On Introducing New Information Technologies in Jurisprudence

Rossijskoe Pravo. Obrazovanie, Praktika, Nauka ◽

10.34076/2410-2709-2019-3-82-86 ◽

2019 ◽

pp. 82-86

Author(s):

Aleksandr Mitin

Keyword(s):

Information Technologies ◽

New Technologies ◽

Professional Competence ◽

Comprehensive Analysis ◽

Legal Services ◽

Degree Programs ◽

Creative Work ◽

Electronic Trading ◽

New Information ◽

Legal Space

The article discusses the possibilities of automation of legal activities. Special attention is paid to the new branch of the business LegalTech, which allows providing legal services using information technology. Some projects in this area are briefly described: FreshDoc document designer, VideoContract app, and electronic trading platforms such as Legal Space and Pravoved.Ru. Although the legal community is not quite ready to work in such conditions, higher education institutions are already reforming their curricula, developing disciplines that allow gaining professional competence in introducing technologies that automate legal work, and so on. The author, in turn, offers using chat bots in legal clinics, gives examples of new disciplines for master’s degree programs, considers the idea of passing final examinations outside universities in certification centers, etc. It is emphasized that in jurisprudence there are a lot of typical situations in which typical decisions need to be made, and here artificial intelligence will be a good helper, and scientists will have more time to undertake a comprehensive analysis of law. Thus, even with the advent of new technologies, the creative work of lawyers will always be in demand.

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