Identifikasi Tumbuhan Paku Air (Azolla sp.) Secara Morfologi dan Molekuler dengan Menggunakan Gen rbcL (Identification of Water Ferns (Azolla sp.) Based on Morphologycal Traits and Molecular Marker Using rbcL Gene)

2018 ◽  
Vol 8 (2) ◽  
pp. 38
Author(s):  
Wianita Mantang ◽  
Feky R. Mantiri ◽  
Beivy J. Kolondam
Keyword(s):  
Genetic Distance ◽  
Morphological Characters ◽  
Morphological Identification ◽  
Rbcl Gene ◽  
Distance Analysis ◽  
Central Java ◽  
North Sulawesi ◽  
Close Relationship ◽  
Water Ferns ◽  
Genetic Distance Analysis

Abstrak Azolla merupakan salah satu tumbuhan paku air yang memiliki banyak manfaat, namun belum banyak dikenal dan sering dianggap sebagai tumbuhan gulma. Pengidentifikasian akan keberadaan jenis-jenis tumbuhan paku air (Azolla sp.) penting untuk dilakukan guna mendukung upaya pengembangan, pembudidayaan dan eksplorasi tumbuhan Azolla sp. Penelitian ini bertujuan untuk mengidentifikasi spesies tumbuhan paku air (Azolla sp.) secara morfologi dan molekuler dengan menggunakan gen rbcL. Hasil penelitian menunjukkan bahwa berdasarkan karakter morfologi sampel tumbuhan Azolla sp. asal Tondano Sulawesi Utara menunjukkan kemiripan dengan spesies A. pinnata dan Azolla asal Magelang Jawa Tengah menunjukkan kemiripan dengan spesies A. microphylla. Identifikasi menggunakan sekuens gen rbcL menunjukkan bahwa sekuens sampel tumbuhan Azolla asal Tondano (WM1) memiliki tingkat kemiripan 100% dengan A. pinnata dan Azolla asal Magelang (WM2) memiliki tingkat kemiripan 100% dengan A. microphylla yang terdapat dalam GenBank. Analisis jarak genetik menunjukkan kedua sampel WM1 dan WM2 memiliki hubungan kekerabatan yang cukup dekat dengan nilai jarak genetik 0,060.Kata kunci: Azolla sp., identifikasi morfologi, identifikasi molekuler, gen rbcL Abstract Azolla is one of the water ferns that has many benefits, but it is not yet widely known and is often regarded as a weed plant. Identification of water ferns (Azolla sp.) is important to be carried out to support the development, cultivation, and exploration of Azolla sp. This study aimed to identify species of aquatic plants (Azolla sp.) morphologically and molecularly using the gene rbcL. The results demonstrated that based on the morphological characters, the Azolla sp. from Tondano, North Sulawesi, showed similarity with species A. pinnata and Azolla from Magelang, Central Java, showed similarity to species A. microphylla. Identification using rbcL gene sequences showed that the sample sequence of plants Azolla from Tondano (WM1) had a 100% similarity level with A. pinnata and Azolla from Magelang (WM2) had a 100% similarity level with A. microphylla available in GenBank. Genetic distance analysis showed that both WM1 and WM2 samples had a close relationship with the genetic distance value of 0.060.Key words: Azolla sp., morphological identification, molecular identification, rbcL gene

Genetic distance analysis among nine endogamous population groups of Maharashtra, India

1979 ◽  
Vol 8 (6) ◽  
pp. 567-570 ◽  
Author(s):  
B.N. Mukherjee ◽  
P.P. Majumder ◽  
K.C. Malhotra ◽  
S.K. Das ◽  
S.L. Kate ◽  
...  
Keyword(s):  
Genetic Distance ◽  
Population Groups ◽  
Distance Analysis ◽  
Endogamous Population ◽  
Genetic Distance Analysis

scholarly journals Whole Genome Characterization and Genetic Evolution Analysis of a New Ostrich Parvovirus

Viruses ◽  
2020 ◽  
Vol 12 (3) ◽  
pp. 334
Author(s):  
Kunpeng Yuan ◽  
Dongdong Wang ◽  
Qingdong Luan ◽  
Ju Sun ◽  
Qianwen Gao ◽  
...  
Keyword(s):  
Comprehensive Analysis ◽  
Open Reading Frames ◽  
Genome Characterization ◽  
Distance Analysis ◽  
Evolution Analysis ◽  
New Information ◽  
Close Relationship ◽  
Goose Parvovirus ◽  
Genetic Distance Analysis ◽  
Reading Frames

Ostrich diseases characterized by paralysis have been breaking out in broad areas of China since 2015, causing major damage to the ostrich breeding industry in China. This report describes a parvovirus detected in ostriches from four different regions. The entire genomes of four parvovirus strains were sequenced following amplification by PCR, and we conducted comprehensive analysis of the ostrich parvovirus genome. Results showed that the length genomes of the parvovirus contained two open reading frames. Ostrich parvovirus (OsPV) is a branch of goose parvovirus (GPV). Genetic distance analysis revealed a close relationship between the parvovirus and goose parvovirus strains from China, with the closest being the 2016 goose parvovirus RC16 strain from Chongqing. This is the first report of a parvovirus in ostriches. However, whether OsPV is the pathogen of ostrich paralysis remains uncertain. This study contributes new information about the evolution and epidemiology of parvovirus in China, which provides a new way for the study of paralysis in ostriches.

Identification and Genetic Distance Analysis of Wheat Cultivars using RAPD Fingerprinting

1997 ◽  
Vol 25 (4) ◽  
pp. 875-882 ◽  
Author(s):  
A. A. Myburg ◽  
A-M. Botha ◽  
B. D. Wingfield ◽  
W. J. M. Wilding
Keyword(s):  
Genetic Distance ◽  
Wheat Cultivars ◽  
Rapd Fingerprinting ◽  
Distance Analysis ◽  
Genetic Distance Analysis

scholarly journals GENETIC DISTANCE OF 47 ACCESSIONS OF OIL PALM (Elaeis guineensis Jacq.) GERMPLASM FROM CAMEROON BASED ON MORPHOLOGICAL CHARACTER

2019 ◽  
Vol 27 (1) ◽  
pp. 25-40
Author(s):  
Sujadi Sujadi ◽  
Tiara S Wandita ◽  
Nanang Supena ◽  
Yurna Yenni
Keyword(s):  
Genetic Diversity ◽  
Cluster Analysis ◽  
Genetic Distance ◽  
Palm Oil ◽  
Oil Palm ◽  
Alternative Energy ◽  
Elaeis Guineensis ◽  
Distance Analysis ◽  
Elaeis Guineensis Jacq ◽  
Genetic Distance Analysis

Oil palm (Elaeis guineensis Jacq.) is a plantation commodity that has an important role in various aspects of life in Indonesia, especially the domestic economy. This is supported by the increasing demand for world palm oil for food needs (edible oil), industrial (oleochemical), and alternative energy sources based on biodiesel. The development of the palm oil industry requires several efforts to achieve increased national productivity, one of which is the utilization of quality seeds supported by the availability of genetic resources (germplasm) that have a high level of genetic diversity. Efforts that can be made for the development of oil palm in Indonesia is through the characterization of oil palm intoduction from abroad. Considering this, further research on genetic distance analysis of palm oil accession introduced from Cameroon is based on morphological characters to produce parent elders that can produce heterosis properties. Research conducted at Seed Garden of Adolina PTPN IV and laboratory analysis of plant material of Palm Oil Research Center located on Jl. Brigjen Katamso No. 5, Medan, North Sumatera, which lasted from December 2017 to February 2018. Single observations were made on 47 accession palm oil from Cameroon planted on December 2010 and ten trees from PPKS 540 variety for compare. Data analysis used was a description of the plant to know the character of plant morphology as well as genetic distance analysis. Analysis of genetic distance using PCA analysis and cluster analysis. The results obtained are based on the results of PCA (Principal Component Analysis) reduce the observed character into six major components that have eigen value > 1 and able to explain the material diversity tested for 73.8%. Based on the cluster analysis obtained the genetic distance of 47 accessions of palm oil from Cameroon by 57%. It can be concluded that, when the genetic distance between accessions is further away, the larger the genetic variability between the observed characters. If the genetic diversity is wider the greater the chance for successful selection in increasing the desired gene frequency.

scholarly journals Characterization of Sentani gudgeon, Oxyeleotris heterodon (Weber, 1907) at Sentani Lake, Papua, Indonesia based on truss morphometric

2018 ◽  
Vol 19 (3) ◽  
pp. 1013-1020
Author(s):  
ABINAWANTO ABINAWANTO ◽  
EKA DEWI SRIYANI
Keyword(s):  
Genetic Distance ◽  
Fish Population ◽  
P Value ◽  
Morphometric Characters ◽  
Distance Analysis ◽  
Dorsal Portion ◽  
Genetic Distance Analysis

Abinawanto, Sriyani ED, Bowolaksono A. 2018. Characterization of Sentani gudgeon, Oxyeleotris heterodon (Weber, 1907) at Sentani Lake, Papua, Indonesia based on truss morphometric. Biodiversitas 19: 1013-1020. The study concerning the morphometry of Sentani gudgeon (Oxyeleotris heterodon, Weber 1907) was done from August 2016 until April 2017 in Sentani Lake, Jayapura District, Papua Province, Indonesia. The objectives of the study were to find out the diversity of the fish using truss morphometry approach. A total 56 individual sampled from three sampling sites were characterized for their 26 truss morphometric characters. The result showed that there were differences on morphometric characters of Sentani gudgeon. The highest correlation value found in character B2 and D5, which represented the dorsal portion and the tail fin of the fish body respectively. All variables showed significant different between each location (the p-value <c0.05). The canonical diagram resulted of 26 truss morphometric characters illustrated three separated clusters which discriminated the three populations based on sampling sites. A genetic distance analysis among Sentani gudgeon indicated that the fish originated from Putali Village was morphologically closed to the fish population from Sosiri. The truss morphometry technique can be used to identify the diversity of Sentani gudgeon in Sentani Lake

scholarly journals Distribution of Selected Single Nucleotide Polymorphisms in the Brahmin, Rajput and Bania Populations of Jammu District of Jammu and Kashmir, North India

2021 ◽  
Vol 21 (02) ◽  
Author(s):  
S. M. S. Chahal
Keyword(s):  
Single Nucleotide Polymorphisms ◽  
Genetic Differentiation ◽  
Genetic Distance ◽  
North India ◽  
Nucleotide Polymorphisms ◽  
Single Nucleotide ◽  
Jammu And Kashmir ◽  
Distance Analysis ◽  
The People ◽  
Genetic Distance Analysis

ABSTRACTA total of 461 randomly selected unrelated subjects belonging to three selected castes populations of Jammu district of Jammu and Kashmir (J&K) viz., the Brahmin, Rajput, and Bania were typed using standard PCRRFLP technique for a battery of five SNPs (Single Nucleotide Polymorphisms) namely NAT2, ADH2, PSCR, T2, and ALAD. The objective of the present study was to characterize these populations genetically and assess the degree of genetic differentiation and genetic affinities among them. The results revealed that the present caste populations were moderately differentiated (GST = 0.0105).The genetic distance analysis demonstrated that the Rajput and Bania were in close genetic affinities while the Brahmin population was somewhat distant. In conclusion, the present investigation documented the underlying genomic uniformity in the people of the Jammu district.

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