scholarly journals Prevalence and Genetic Characterisation of Human Sapovirus from Children with Diarrhoea in the Rural Areas of Vhembe District, South Africa, 2017–2020

Viruses ◽  
2021 ◽  
Vol 13 (3) ◽  
pp. 393
Author(s):  
Mpho Magwalivha ◽  
Jean-Pierre Kabue Ngandu ◽  
Afsatou Ndama Traore ◽  
Natasha Potgieter

Diarrhoeal disease is considered an important cause of morbidity and mortality in developing areas, and a large contributor to the burden of disease in children younger than five years of age. This study investigated the prevalence and genogroups of human sapovirus (SV) in children ≤5 years of age in rural communities of Vhembe district, South Africa. Between 2017 and 2020, a total of 284 stool samples were collected from children suffering with diarrhoea (n = 228) and from children without diarrhoea (n = 56). RNA extraction using Boom extraction method, and screening for SV using real-time PCR were done in the lab. Positive samples were subjected to conventional RT-PCR targeting the capsid fragment. Positive sample isolates were genotyped using Sanger sequencing. Overall SV were detected in 14.1% (40/284) of the stool samples (16.7% (38/228) of diarrhoeal and 3.6% (2/56) of non-diarrhoeal samples). Significant correlation between SV positive cases and water sources was noted. Genogroup-I was identified as the most prevalent strain comprising 81.3% (13/16), followed by SV-GII 12.5% (2/16) and SV-GIV 6.2% (1/16). This study provides valuable data on prevalence of SV amongst outpatients in rural and underdeveloped communities, and highlights the necessity for further monitoring of SV circulating strains as potential emerging strains.

2019 ◽  
Author(s):  
Mpumelelo Casper Rikhotso ◽  
Ronewa Khumela ◽  
Jean Pierre Kabue ◽  
Afsatou Ndama Traoré ◽  
Natasha Potgieter

AbstractBACKGROUNDAcute gastroenteritis (AGE) is a leading cause of morbidity and mortality in young children worldwide. Human Bocavirus (HBoV) is an emerging virus globally associated with diarrhea. The aim of this study was to demonstrate the prevalence of HBoV genotypes in children (≤5 years) from rural communities in South Africa (SA) suffering from AGE.MATERIAL AND METHODA total of 141 fecal samples of children ≤5 years with acute gastroenteritis (AGE) were collected from rural Primary Health Care facilities in the Vhembe district of SA between June 2017 and July 2018. Clinical symptoms and demographic data were also recorded. A total of 102 (72%) were outpatients and 39 (28%) were hospitalized patients. Human Bocavirus (HBoV) genotypes were determined using Real-Time Multiplex PCR. DNA extracts of positive samples were confirmed by conventional PCR targeting the NS1 gene. Co-infection with other enteric viruses were determined in HBoV positive samples using Real-Time PCR.RESULTSHBoV was detected in 8 (5.7%) children with AGE. Children were in the age group between 1-24 months. HBoV1 and HBoV3 genotypes were each detected in 3 (37.5%) stool samples and HBoV2 in 2 (25%) stool samples. Co-infection with other enteric viruses included Rotavirus (37.5%); Adenovirus (37.5%); Norovirus (25%) and Astrovirus (12.5%).CONCLUSIONHBoV infections could be seen as a potential emerging diarrheal pathogen in South Africa. Further studies are required to understand the role of HBoV infections in children and adults with acute gastroenteritis.Author summaryAcute gastroenteritis (AGE) is recognized as a major cause for mortality in children ≤5 years of age in Africa and other developing countries. Viruses known to be involved in AGE includes Rotavirus, Norovirus, Astrovirus and Adenovirus and have been reported globally. Recently the Human Bocavirus (HBoV) have been reported in numerous studies globally as a potential cause of diarrhea. In this study, the prevalence and genetic diversity of human Bocavirus in children with AGE from rural communities in Limpopo, South Africa were investigated. In total, 141 stool samples from children ≤ 5 years with AGE were assessed for the presence of HBoV using Real-Time PCR. HBoV were detected in 8 (5.7%) patients and included 3 positive samples for HBoV1 and HBoV3 respectively and 2 positive for HBoV2. No HBoV4 were detected. Among the 8 positive HBoV samples, co-infection with other enteric viruses were found in 7 (87.5%) samples, while mono infection with HBoV alone was detected in 1 (12.5%) patient. HBoV mixed infection with Rotavirus (3/8; 37.5%); Adenovirus (3/8; 37.5%); Norovirus (2/8; 25%) and Astrovirus (1/8; 12.5%) were observed in this study. This study reported for the first time on the prevalence of human Bocavirus in children with AGE from rural communities in South Africa.


2021 ◽  
Vol ahead-of-print (ahead-of-print) ◽  
Author(s):  
Azwindini Isaac Ramaano

PurposeThis study evaluates tourism implications and challenges around the Big Tree Nature Reserve with its adjacent tourism ventures and entities in Musina Municipality, Limpopo Province, in South Africa.Design/methodology/approachData got compiled by interviews and questionnaires in addition to focus group discussions and physical observation.FindingsThe study revealed many challenges associated with tourism undertakings around the Big Tree Nature Reserve and its nearby tourism entities. The benefits of tourism were not efficiently tapped into by the local communities within and around the study area. The factors contributing to the poor state of tourism running got delineated by the findings of the study. Hence, there was a potential obligation for a turnaround effective tourism strategy to improve tourism enterprises to benefits local communities within the area.Originality/valueMusina Municipality is one of the remote regions in the North of Limpopo Province, in South Africa. It also has impoverished rural communities akin to many rural areas within the continent. Despite the mentioned, it considerably covers a mostly tourism-orientated area within the Vhembe District.


2020 ◽  
Vol 54 (3) ◽  
pp. 761-798
Author(s):  
Iwara Obaeko ◽  
Faith Musvipwa ◽  
Amaechi Ekene ◽  
Raymond Raselekoane

National responses to the COVID-19 pandemic have varied from country to country. In South Africa, the response has included a compulsory 21-day lock-down entailing restrictions of social and economic activities among other things. This arguably helped the country to avoid further spread of the virus, especially in townships, informal settlements and rural communities, where access to health facilities is often difficult. However, it has also exposed rural households to unforeseen challenges. This paper explores these challenges in view of proffering policy measures to help such households during subsequent lock-down. University students living within such rural households were purposively sampled to solicit electronic data from heads of households in Vhembe district. Using a qualitative method to analyze the data gathered from 82 households, five major issues emerged which include access to basic needs such as groceries, whereas water and health items were some of the challenges. The situation in households is further compounded by 'limited source of income' due to retrenchments and shutdown of subsistence businesses. 'Depression and frustration' emanating from the fear of contracting the virus, spousal domestic abuse, inability to meet home obligations, family squabbles as well as boredom caused by movement restriction also constitute part of the challenges. The lack of needed information regarding the virus, and 'theft' were other two challenges. Based on these findings, the paper recommends thorough consideration of the identified challenges before the enforcement of such lock-down. It also encourages more improvement to be made in the area of service delivery in rural communities.


Water SA ◽  
2018 ◽  
Vol 44 (3 July) ◽  
Author(s):  
DC Sambo ◽  
A Senzanje ◽  
K Dhavu

Some parts of the rural areas of South Africa are receiving water services below the legislative standards stipulated in the Water Services Act (1997) of South Africa. This is because small-scale water infrastructures (SWI), including standpipes, handpumps and windmills, are failing to supply adequate water in rural communities for various activities that enhance their livelihoods. This is due to technical, community, institutional, and environmental factors. Literature indicates that these factors are complex in nature. However, research on their complex interactions has been limited. Therefore, the complex interactions of the factors causing SWI failure were investigated and analysed in this study. A qualitative research approach was employed to investigate the factors that cause failure of SWI. The network approach combined with graph theory and the community structure method were used to generate a theme and domain network that allowed for a systematic analysis and interpretation of the causes of SWI failure in the study area. The major causes of failure identified include: (i) the use of a top-down approach to implement water projects in rural communities without consultation, (ii) sharing of SWI in high numbers due to the limited number of SWI available/functional, resulting in overloading and increased breakdowns, and (iii) drying up of water sources due to poor siting leading to vandalism of SWI. Overall, the causes of SWI failure are as a result of the complex interactions of different categories of factors. The theme and domain network is a powerful tool that can be used represent and analyse the complex interactions. It is therefore imperative for interventions aiming to improve rural water supply to analyse the complex interactions of the causes of SWI failure to understand the underlying problems at a level of a system, in order to propose suitable solutions.


2019 ◽  
Author(s):  
Lunic Base Khoza ◽  
Wilfred Njabulo Nunu ◽  
Bumani Solomon Manganye ◽  
Pfungwa Mambanga ◽  
Shonisani Tshivhase ◽  
...  

Abstract Background Despite government efforts to improve access to health care services through the re-engineered Primary Health Care and National Health insurance platform, access still remain a challenge particularly in rural areas. The aim of this study was to analyse secondary data on cataract patients who were attended to in selected hospitals in rural Limpopo of South Africa. Methodology A cross section survey was conducted on 411 patient records from five selected hospitals in Vhembe district. A pre tested structured checklist was used to guide retrieval of variables from patient records. The collected data was entered into excel spreadsheet, cleaned and imported into Statistical Package for Social Sciences version 26 for analysis. Proportions of demographic characteristics were presented and these were cross tabulated with the outcome variable “success of operation” using Chi Squared tests. Results Findings point out that majority of patients who attended hospital for eye services were aged 65 years above and females (63%). There was no association between the tested demographic characteristics and the outcome variable. Most patients were diagnosed in the period 2015-2018 (60%). Over 90% of those that were operated had successful operations. Of the remaining 10% that had unsuccessful operations, 30% cited complications as being the reason why these operations were unsuccessful. Conclusions It is evident from the findings that cataract services offered in rural areas have low impact as they are not accessible to the patient. It is critical to have a worker retention strategy to retain experts.


Author(s):  
Sharol Mkhomazi

The deployment of telecommunication infrastructures is a challenge in many parts of South Africa particularly in the rural areas. The challenge has impact of communities' members as they do not have network coverage for Internet in some areas. The challenge gets worse with individual telecommunication service provider. Hence there is technological proposal for sharing of infrastructure by the service providers. However, the sharing of infrastructure is not as easy as notion by many individuals and groups institutions included. The article presents findings from a study on how a South African telecommunication network service provider could deploy shared infrastructures in the country's rural communities. The sharing of infrastructure is described by the structure and actions of agents within the infrastructure sharing process. Structuration theory was employed as a lens in the data analysis. The key findings include insufficient distribution of infrastructure, ownership responsibility, competitiveness, infrastructure deployment cost, and signification of regulation.


2018 ◽  
Vol 20 (1) ◽  
Author(s):  
Namadzavho Joyce Muswede ◽  
Azwihangwisi Helen Mavhandu-Mudzusi

Condoms are considered useful in the prevention of unplanned pregnancies and sexually transmitted infections including the human immunodeficiency virus. However, condom use is a challenge among young adults in rural areas. This paper reports the views of young adults regarding the strategies which can be utilised for promoting condom use in one of the rural clinics in the Vhembe district, Limpopo, South Africa. This quantitative, cross-sectional, descriptive study was conducted in one of the clinics in the Vhembe district. Data were collected using structured questionnaires administered to 372 young adults who came to visit the clinic. Data analysis was done using Statistical Package for Social Sciences (SPSS) version 28. Results indicate that condom use among the youth can be promoted though (i) improving the negative attitudes of healthcare providers to condom issuing and use, (ii) involving other stakeholders who have an impact on the use of condoms, and (iii) promoting condom use. Retraining of healthcare providers to improve negative attitudes is a matter of priority. In-service training should focus on disseminating information about patients’ rights. The following recommendations were put forth: enhancement of communication strategies for marketing condom use, broadening sex education in the school curriculum, and improving the negative attitudes of healthcare providers to the promotion of condom use.


Author(s):  
Varoshini Nadesan

This study highlights the challenges faced by social work students during their fourth-year field placements. The research was conducted among social work students at two historically disadvantaged universities in South Africa. The aim of the study was to understand the field experiences of final-year Bachelor of Social Work students from historically disadvantaged universities. It was anticipated that these students were placed at rural or semi-rural communities that were close to the university. Students in the fourth and final year of their undergraduate study are expected to receive training that would generate advanced field practice experience in specialised settings to prepare them for the reality of social work practice in the workplace. A qualitative study was undertaken among current and past social work students. The findings highlighted issues pertaining to limited placements, poorly managed student placements, access to communities, and supervision challenges. However, the key findings indicate that once placed, the students felt abandoned by their training institutions and left to rely solely on their field supervisors for academic, administrative and developmental guidance. The study recommends that universities be more adept at fulfilling the needs of students and providing comprehensive support. The study also recommends that a suitably administered system of communication between the university, student and placement agency be implemented as part of the comprehensive support to students.


Sign in / Sign up

Export Citation Format

Share Document