scholarly journals Effect of Different Routes of Vaccination against Aeromonas salmonicida on Rearing Indicators and Survival after an Experimental Challenge of Pikeperch (Sander lucioperca) in Controlled Rearing

Vaccines ◽  
2020 ◽  
Vol 8 (3) ◽  
pp. 476
Author(s):  
Patrycja Schulz ◽  
Elżbieta Terech-Majewska ◽  
Andrzej Krzysztof Siwicki ◽  
Barbara Kazuń ◽  
Krystyna Demska-Zakęś ◽  
...  

Bacterial diseases are a significant problem in the controlled rearing of fish. Furunculosis (Aeromonas sp.), flavobacteriosis (Flavobacterium sp.), and pseudomonadosis (Pseudomonas sp.) are currently the most frequently identified diseases in recirculating aquaculture systems of various fish species. Such a situation is also observed in pikeperch rearing. Due to the emerging difficulties of effective prophylaxis using commercial vaccines, interest in the use of autovaccinations is increasing, not only in ichthyopathology but also in other veterinary fields. Our research aimed to assess the effect of the vaccination method on the overall condition of the fish and survival after the experimental infection with Aeromonas salmonicida. Pikeperch were vaccinated by (1) bath, (2) a single i.p. injection, or (3) feed. The fish were measured and weighed on day 0 and after 28 and 56 days of the experiment. Specific growth rate, daily growth rate, condition factor, and feed conversion ratio were calculated. On days 7, 14, 21, and 28 of the experiment, ceruloplasmin and lysozyme levels were rated. In addition, a challenge test was performed. The obtained results showed that the method of vaccination is important and affects the growth of fish, the overall condition of fish, and survival after experimental infection.

Author(s):  
Farida . ◽  
Eka Indah Raharjo ◽  
Arnis Maylinda Sari

ABSTRAKPenelitian bertujuan untuk mengetahui penggunaan fermentasi bungkil kelapa sawit dalam pakan buatan.Penelitian menggunakan Rancangan Acak Lengkap (RAL) yang terdiri dari 4 perlakuan 3 ulangan.Susunan perlakuan adalah Perlakuan A : bungkil kelapa sawit 12%, Perlakuan B : fermentasi bungkil kelapa sawit 15%, Perlakuan C : fermentasi bungkil kelapa sawit 18%, Perlakuan D : fermentasi bungkil kelapa sawit 21%. Penelitian ini dilakukan selama 60 hari untuk mengetahui laju pertumbuhan spesifikberat, laju pertumbuhan harianpanjang, konversi pakan, kelangsungan hidup dan kualitas air sebagai penunjang.Hasil penelitian menunjukkan pemberian pakan buatan dengan fermentasi bungkil kelapa sawit menghasilkan respon yang baik terhadap pertumbuhan ikan gurami. Pemberian pakan buatan dengan fermentasi bungkil kelapa sawit 18% (perlakuan C), memberikan hasil terbaik dengan laju pertumbuhan dengan rata – rata laju pertumbuhan spesifik berat 1.502%, laju pertumbuhan harian panjang 4.06%, nilai konversi pakan rata – rata 3.48 dan kelangsungan hidup rata – rata 90%.Kata Kunci : Fermentasi Bungkil Kelapa Sawit, Ikan Gurami, Laju PertumbuhanThe study aims to determine the use of fermentation residue oil palm in artificial feed .Research using a completely randomized design ( CRD ), which consists of 4 treatment three replications.The composition of the treatment is the treatment  A : 12 % of palm oil cake , Treatment B : fermented palm oil cake 15 % , Treatment C : fermented palm oil cake 18 % , Treatment D : fermentation residue palm oil 21 %.This research was conducted for 60 days to determine the specific growth rate of heavy , long daily growth rate , feed conversion , survival and quality of water as a supporter.The results showed the artificial feeding with fermented palm cake to produce a good response to the growth of carp.Feeding artificially by fermentation cake palm oil 18 % ( treatment C ) , gives the best results with a growth rate with the average - average growth rate of the specific weight of 1.502 % daily growth rate long- 4:06 % , the value of feed conversion average - average 3:48 and median survival - average of 90 % .Keywords : Fermentation Palm Kernel Oil, Fish carp, Growth Rate


2017 ◽  
Vol 8 (2) ◽  
pp. 747
Author(s):  
Fazril Saputra ◽  
Dinamella Wahjuningrum ◽  
Kustiariyah Tarman ◽  
Irzal Effendi

<em>This study aimed to determine the best dose of administration of marine fungal <span style="text-decoration: underline;">Nodulisporium</span> sp. KT29 metabolites to improve the production performance of marine culture of white shrimp. The experimental animals used were white shrimp <span style="text-decoration: underline;">Litopenaeus</span> <span style="text-decoration: underline;">vannamei</span> post larvae 12, which were reared in the sea using floating net cages with a stocking density of 700 post larvae/net. Experimental design used was a completely randomized design consisting of 3 treatments with 3 replications; control without the administration of <span style="text-decoration: underline;">Nodulisporium</span> sp. KT29 metabolites, dietary of feed containing <span style="text-decoration: underline;">Nodulisporium</span> sp. KT29 metabolites at doses of 20 mL/kg of feed and 40 mL/kg of feed. The results showed that survival rate, absolute length growth, weight gain, daily growth rate and feed conversion ratio in treatment groups were better than the control (P&lt;0.05). The administration of marine fungal <span style="text-decoration: underline;">Nodulisporium</span> sp. KT29 metabolites at a dose of 20 mL/kg of feed could improve the production performance of white shrimp cultivated in the sea with survival rate, daily growth rate and feed conversion ratio: 66.61±6.94%, 20.18±0.39 %/day and 3.20±0.22, respectively.</em>


2021 ◽  
Vol 21 (3) ◽  
pp. 93-100
Author(s):  
Arning Wilujeng Ekawati ◽  
Siti Mariyah Ulfa ◽  
Citra Satrya Utama Dewi ◽  
Abdul Aziz Amin ◽  
Lutfi Ni’matus Salamah ◽  
...  

Water quality and waste management are significant problems for aquaculture in Indonesia, including catfish (Clarias gariepinus) aquaculture. A combination of Aquaponics Systems and Recirculating Aquaculture Systems (A-RAS) has been developed to address this problem. This study analyzed A-RAS application for catfish (Clarias gariepinus) aquaculture by comparing water quality and production indicators between A-RAS and conventional methods. The results show that temperature, pH, dissolved oxygen, total organic matter, ammonia, nitrate and nitrite in A-RAS technology were 28.0-30.0°C, 6.5-7.9, 3.8-7.8 mg/L, 18.54-24.97 mg/L, 0.12- 0.28 mg/L, 0.12-0.13 mg/L, and 0.04-0.13 mg/L, respectively. Survival Rate, Feed Conversion Ratio, and harvest in A-RAS application were 85.5%, 1.1, and 26 kg/m³, respectively. A-RAS technology can maintain water quality to be reused in cultivation and increase yields by about 13%. Besides, water spinach can be an additional income for farmers.


Author(s):  
Choirun Nissa Ramadhani ◽  
Yuli Andriani ◽  
Ibnu Bangkit Bioshina Suryadi ◽  
Kiki Haetami

This research aims to determine the percentage of the addition of fermented lamtoro leaf on fish growth rate of gourami fingerlings. The test fish used was gourami that amounted to 320 tails with a length of 4-6 cm from Farming Development Gurame and Nilem – Singaparna, Tasikmalaya. The container used in this research was an aquarium with a total of 16 pieces with a size of 40 × 30 × 30 cm3. The density of gourami fingerlings during the research was 20 fish-tails/aquarium with long maintenance of 40 days. The feed given is 5% of the fish body biomass. The research was conducted on 8 November 2019 until 14 February 2020 at the Fisheries Laboratory, Faculty of Fisheries and Marine Sciences, Universitas Padjadjaran, Indonesia. This research used experimental method with Completely Random Design (CRD), which consists of four treatments and four times the repeated feeding of commercial feed (control), the leaf meal of lamtoro fermentation result 10%, 15%, and 20%. Based on the results of the research, fermentation of lamtoro leaves can improve nutrient content after fermentation, seen from the presence of increased protein content from 21.88% to 26.11% and decrease in crude fiber content from 46.33% to 20.85%. The addition of lamtoro leaf meal fermentation results up to 15% in commercial feed resulting in a daily growth rate of 1.90%, feed conversion ratio of 2.05 and the survival rate of 70%.


2017 ◽  
Vol 8 (2) ◽  
pp. 747-755 ◽  
Author(s):  
Fazril Saputra ◽  
Dinamella Wahjuningrum ◽  
Kustiariyah Tarman ◽  
Irzal Effendi

This study aimed to determine the best dose of administration of marine fungal Nodulisporium sp. KT29 metabolites to improve the production performance of marine culture of white shrimp. The experimental animals used were white shrimp Litopenaeus vannamei post larvae 12, which were reared in the sea using floating net cages with a stocking density of 700 post larvae/net. Experimental design used was a completely randomized design consisting of 3 treatments with 3 replications; control without the administration of Nodulisporium sp. KT29 metabolites, dietary of feed containing Nodulisporium sp. KT29 metabolites at doses of 20 mL/kg of feed and 40 mL/kg of feed. The results showed that survival rate, absolute length growth, weight gain, daily growth rate and feed conversion ratio in treatment groups were better than the control (P<0.05). The administration of marine fungal Nodulisporium sp. KT29 metabolites at a dose of 20 mL/kg of feed could improve the production performance of white shrimp cultivated in the sea with survival rate, daily growth rate and feed conversion ratio: 66.61±6.94%, 20.18±0.39 %/day and 3.20±0.22, respectively.


Author(s):  
Rafi Satriawan ◽  
. Iskandar ◽  
B. S. Ibnu Bangkit ◽  
Walim Lili

This research aims to determine the application of different water currents in a round container that can provide the most optimal effect on the growth rate of giant gourami (Osphronemus goramy) fingerlings. The method used in this research was an experimental method using a completely randomized design (CRD), which consists of three treatments and four replicates The treatment was the use of aeration stone in a round container A (control), B (water current 0.1 m s-1 in a round container) and C (water current 0.1 m s-1 combined with a venturi in a round container). The fish size used in this research was 2-3 cm obtained from Gourami and Nilem Breeding Centre, Singaparna, West Java Province. Fish were kept in water gallons with a volume of 19 L. Each gallon contained 30 fish. The feeding rate was 5% from biomass. The feed was given three times daily. Every ten days the weight data were collected and used for adjusting the feeding rate. The water quality during the research (40 days) was observed every seven days while survival rate, absolute weight growth, daily growth rate, feed conversion ratio and feed efficiency were observed every ten days. Based on the results of the research, the best reuslt was treatment C (water current of 0.1 m s-1 combined with venturi in a round container) had the highest survival rate (SR) of 58%, absolute weight growth (3.5 g), daily growth rate (1.69%), feed conversion ratio (2.01) and feed efficiency (50%).


Author(s):  
Yuli Andriani ◽  
Fikri Khairun Akbar ◽  
Zahidah . ◽  
Rita Rostika ◽  
Kiki Haetami ◽  
...  

This study aimed to understand the effects of various energy-protein ratios of feed on nilem carp fish (Osteochilus hasselti) growth. This study was conducted from September to October 2017 at the Laboratory of Building 4, Faculty of Fisheries and Marine Sciences, Universitas Padjajaran. The average size of the test fish was ± 7 cm with an average weight of 3 grams. This was a Completely Randomized Design (CRD) experimental study consisting of 5 treatments and 3 repetition. The treatments given were 5 feed formulas based on different feed protein energy ratios, i.e. treatment A (31% protein, ratio 10), treatment B (31% protein, ratio 8), treatment C (28% protein, ratio 10), D (28% protein, ratio 8), and E (30% protein commercial feed/control). Feed, 5% of the test fish total weight, was provided 3 times a day. Parameters observed were daily growth rate, feed conversion, and survival. Data gained were analyzed using F-test, followed by Duncan Test if differences were found between treatments. Results showed that growth rate and feed conversion of all treatments were not different compared to control. The highest result was found in treatment A (31% protein, ratio 10) with a daily growth rate of 3.99% and feed conversion of 1.95%.


2017 ◽  
Vol 16 (1) ◽  
pp. 101 ◽  
Author(s):  
Rita Rostika ◽  
Yuli Andriani ◽  
Arthur Henry Abram ◽  
Apriana Vinasyiam

<p class="Pa3"><strong>ABSTRACT </strong></p><p> </p><p class="Pa5">This research aimed to evaluate the supplementation of fermented <em>Lemna </em>sp. meal (FLM) in the artificial diet on the growth rate of Nile tilapia <em>Oreochromis niloticus</em>. The research design used was complete randomized design consisting of four treatments; A (0% FLM), B (20% FLM), C (30% FLM), and D (40% FLM) in triplicates. The parameters observed were the nutritional composition of LFM, daily growth rate (DGR), feed conversion ratio (FCR), survival rate (SR), and water quality. Fermentation with Effective Microorganism 4 (EM4) has increased crude protein and crude lipid of FLM respectively 5.60% and 5.76%. However it decreased the crude fiber of FLM up to 15.27%. The result suggested that supplementation of FLM 0–40% in the artificial diet could give DGR 0.81–1.20%, FCR 2.48–2.97; and SR 72%–84% in Nile tilapia. The addition of 40% LFM showed the best result among all treatments.</p><p> </p><p class="Pa5">Key words: <em>Lemna </em>meal, EM4, fermentation, Nile tilapia fry</p><p> </p><p> </p><p class="Pa3"><strong>ABSTRAK </strong></p><p> </p><p class="Pa5">Tujuan dari penelitian ini adalah untuk mengevaluasi penggunaan tepung <em>Lemna </em>sp. fermentasi (TLF) pada pakan buatan terhadap laju pertumbuhan ikan nila <em>Oreochromis niloticus</em>. Rancangan penelitian yang digunakan adalah rancangan acak lengkap (RAL) terdiri atas empat perlakuan, yaitu A (0% TLF), B (20% TLF), C (30% TLF), dan D (40% TLF) dengan masing-masing tiga ulangan. Parameter yang diamati dalam penelitian antara lain nilai nutrisi TLF, laju pertumbuhan harian (LPH) ikan nila, rasio konversi pakan (RKP), sintasan (SR), dan kualitas air. Fermentasi dengan <em>Effective Microorganism </em>4 (EM4) meningkatkan protein kasar dan lemak kasar TLF sebesar masing-masing 5,60% dan 6,76%, akan tetapi menurunkan serat kasar hingga 15,27%. Hasil penelitian menunjukkan bahwa pemberian TLF 0–40% dalam pakan komersial menghasilkan LPH 0,81–1,20%; RKP 2,48– 2,97; dan SR 72%–84%. Pemberian TLF sebanyak 40% merupakan perlakuan yang menunjukkan hasil terbaik.</p><p> </p><p>Kata kunci: tepung <em>Lemna</em>, EM4, fermentasi, benih ikan nila</p>


F1000Research ◽  
2020 ◽  
Vol 9 ◽  
pp. 1274
Author(s):  
Firdus Firdus ◽  
Samadi Samadi ◽  
Abdullah A. Muhammadar ◽  
Muhammad A. Sarong ◽  
Zainal A. Muchlisin ◽  
...  

Background: Research on supplementing feed with rice husk activated charcoal was carried out to determine the effect of variations in the concentration of rice husk activated charcoal on the growth and histological features of the Caranx ignobilis intestine. Methods: This study used an experimental method with a completely randomized design consisting of six treatments and four replications, including adding activated charcoal to feed at concentrations of 0%, 1%, 1.5%, 2%, 2.5%, and 3% for 42 days. The measured parameters included daily growth rate (DGR), specific growth rate (SGR), absolute growth rate (AGR), feed conversion ratio (FCR), feed efficiency (FE), survival rate (SR), length of foveola gastrica, width of foveola gastrica, length of intestinal villi, and width of intestinal villi. Data were analyzed statistically using one-way analysis of variance and Duncan’s test. Results: The results showed that supplementing fish feed with rice husk activated charcoal at different concentrations significantly affected the values of DGR, AGR, FCR, FE, SR, length of the foveola gastrica, length of the villous intestine, and width of the villous intestine, but did not significantly affect SGR or foveola gastrica width. Conclusion: The 2% rice husk activated charcoal treatment showed the best results for all parameters.


Author(s):  
Santika Ahmad ◽  
Ibnu Bangkit ◽  
. Iskandara ◽  
Izza Mahdiana Apriliani

This research was purposed is to find the optimum current for growth and growth of Bonylip Barb fingerlings (Osteochilus hasselti C.V). Method used in this research is compelitely Randomized Design (CRD), consists of three treatments and four replications. The treatments are : A (without current or (control), B (with 0.1 ms-1 water current) and C. (0.1 ms-1 water current cabined with venture aeration). Bonylip Barb of 4 – 6 cm size are kept as many as 15 fish / x Liters and the amount of feed given is adjusted to the feeding rate (FR) of 10 % of Bonylip Barb biomass carried out every three times a day and adjusted for growth every ten days. Water quality during maintenance (40 days) was observed once every 10  days while survival, absolute weight growth, daily growth rate, feed conversion ratio and feed efficiency were observed every ten days. After 40 days of maintenance,  the best result showed 0.1 ms-1, with 100% survival rate , 6.09 g absolute growth, 3.13% specific growth rate , 3.48% feed conversion ratio was , and  30.04%. feed efficiency.


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