scholarly journals Influences of Dimethyl Phthalate on Bacterial Community and Enzyme Activity in Vertical Flow Constructed Wetland

Water ◽  
2021 ◽  
Vol 13 (6) ◽  
pp. 788
Author(s):  
QiaoLing Xu ◽  
ShaoYong Lu ◽  
Tao Yuan ◽  
Feng Zhang ◽  
Li Wang ◽  
...  
Keyword(s):  
Enzyme Activity ◽  
Bacterial Community ◽  
Constructed Wetland ◽  
Lower Layer ◽  
Phthalate Esters ◽  
Good Choice ◽  
Pennisetum Purpureum ◽  
Vertical Flow ◽  
Dimethyl Phthalate ◽  
High Concentration

Dimethyl phthalate (DMP), belonging to the family of Phthalate esters (PAEs), is a plasticizer and has been widely used in the world for many years. Nowadays, it has become a ubiquitous environmental pollutant and is listed as an environmental priority pollutant by China’s Environmental Monitoring Center. The purpose of this study is to estimate the responses of the bacterial community and enzyme activity to DMP contamination in three vertical flow constructed wetlands (VFCW), namely the constructed wetland A (planted with Pennisetum sinese Roxb), constructed wetland B (planted with Pennisetum purpureum Schum.), and constructed wetland C (unplanted), respectively. The results showed that the relative percentages of some genera associated with nitrogen metabolism and the function of degrading aromatic hydrocarbons were increased by DMP contamination, such as Dechloromonas agitata, Pleomorphomonas sp., Denitratisoma oestradiolicum, Plasticicumulans lactativorans, Novosphingobium sp., Alicycliphilus denitrificans, and Thauera sp. Meanwhile, principal coordinate analysis (PCA) analysis showed that the addition of DMP divided 12 samples into two groups as followed: one was the DMP group containing a-1, a-2, b-1, b-2, c-1 and c-2 while the other was no DMP group including A-1, A-2, B-1, B-2, C-1 and C-2. It indicated that DMP was the main reason for this change. In addition, by monitoring the activity of substrate enzymes, the activity of urease, phosphatase, catalase, and invertase in the wetlands before and after the experiment, these were significantly higher in the upper layer than in the lower layer and maintained high activity. Ultimately, the average influent concentration of DMP in three VFCWs was 8.12 mg/L and the average removal efficiency of the effluent was over 90%. Our results suggested that DMP was an important factor affecting the microbial community structure of wetland and the upper layer of the VFCW was the main site for the degradation of DMP. VFCW has great potential for the removal of the high concentration of DMP and it can be a good choice for the treatment of PAEs.

Small scale recirculating vertical flow constructed wetland (RVFCW) for the treatment and reuse of wastewater

2008 ◽  
Vol 58 (2) ◽  
pp. 487-494 ◽  
Author(s):  
A. Gross ◽  
M. Y. Sklarz ◽  
A. Yakirevich ◽  
M. I. M. Soares
Keyword(s):  
Constructed Wetland ◽  
Root Zone ◽  
Lower Layer ◽  
Plant Root ◽  
Low Cost ◽  
Treatment Method ◽  
Organic Soil ◽  
Small Scale ◽  
Vertical Flow ◽  
Vertical Flow Constructed Wetland

The quantity of freshwater available worldwide is declining, revealing a pressing need for its more efficient use. Moreover, in many developing countries and lightly populated areas, raw wastewater is discarded into the environment posing serious ecological and health problems. Unfortunately, this situation will persist unless low-cost, effective and simple technologies are brought in. The aim of this study is to present such a treatment method, a novel setup which is termed recirculating vertical flow constructed wetland (RVFCW). The RVFCW is composed of two components: (i) a three-layer bed consisting of planted organic soil over an upper layer of filtering media (i.e. tuff or beads) and a lower layer of limestone pebbles, and (ii) a reservoir located beneath the bed. Wastewater flows directly into the plant root zone and trickles down through the three-layer bed into the reservoir, allowing passive aeration. From the reservoir the water is recirculated back to the bed, several times, until the desired purification is achieved. The results obtained show that the RVFCW is an effective and convenient strategy to treat (domestic, grey and agro) wastewater for re-use in irrigation. The system performance is expected to be further improved once current optimization experiments and mathematical modeling studies are concluded.

Water Budget of Constructed Wetland System with Subsurface Vertical Flow in Sub-Humid Tropical Climate

2015 ◽  
Vol 1 (5) ◽  
pp. 235-242
Author(s):  
Édio Damásio da Silva Júnior ◽  
Rogério de Araújo Almeida ◽  
Elisa Rodrigues Siqueira ◽  
Ábio Roduvalho da Silva
Keyword(s):  
Constructed Wetland ◽  
Water Budget ◽  
Tropical Climate ◽  
Vertical Flow ◽  
Humid Tropical Climate ◽  
Wetland System

Effect of nitric oxide on Sertoli cell microtubule of piglets

2009 ◽  
Vol 6 (3) ◽  
pp. 257-263 ◽  
Author(s):  
Yang Li ◽  
Wang Xian-zhong ◽  
Yang Meng-bo ◽  
Zhang Jia-hua
Keyword(s):  
Nitric Oxide ◽  
Enzyme Activity ◽  
Protein Kinase ◽  
Cell Viability ◽  
Sodium Nitroprusside ◽  
Sertoli Cell ◽  
Sertoli Cells ◽  
High Concentration ◽  
Treatment Results ◽  
Anti Oxidant

AbstractTo illustrate the effect of nitric oxide (NO) on the microtubules of Sertoli cells (SC), SCs of piglets were treated with sodium nitroprusside (SNP). Changes in cell viability, anti-oxidant activity, enzyme activity and p38 mutagen-activated protein kinase (p38MAPK) activation were detected. The results were as follows. A low concentration of NO can keep SC microtubule and cell viability normal, and a high concentration of NO could increase p38MAPK activation, decrease anti-oxidant activity and transferrin secretion, and destroy the structure and distribution of the microtubules. The results suggest that SNP treatment results in an increase in NO in SCs and decreased cell anti-oxidant activity. The high concentration of NO destroys cell microtubules by activating p38MAPK.

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