Method for quantitative assessment of surgical wound healing (for example, the hole of a removed tooth). Part I

Relevance. Surgical stages of the dental implant restoration are an indispensable part of the treatment procedures. However, underestimation of residual/ available alveolar bone volume after tooth extraction may challenge implantation planning. Socket healing process might depend on either a surgical technique, or local or systemic preexisting factors, that this study should present as quantitative data. Purpose is to develop a technique for quantitative assessment of a surgical wound healing, e.g. an extraction socket.Materials and methods. The study included 42 patients after extraction of different teeth during the preparatory procedures for further dental arch restoration with implants. Follow-ups, clinical data, laboratory and radiographic findings determined clinical, radiographic, and information values – dependent and independent variables as quantifiable clinical parameters – biomarkers; they were statistically analyzed.Results. The conducted analysis revealed correlation dependence between selected parameters. Highly significant interrelationship allowed calculating the overall success rate of wound/ socket healing after tooth extraction. A regression model was developed to explain 76% healing success rate.Conclusion. Thus, factors and conditions determining the healing of a surgical bone wound, e.g. an extraction socket, consistently affect each other. Numerical sequences of real-life processes characterize the extent and highly significant interrelations and allow predicting the mutual impact of the factors.

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The effect of Persea americana Mill. seed extract on inflammatory cells and fibroblast formation in tooth extraction socket healing

Dental Journal (Majalah Kedokteran Gigi) ◽

10.20473/j.djmkg.v54.i4.p190-194 ◽

2021 ◽

Vol 54 (4) ◽

pp. 190

Author(s):

Yessy Ariesanti ◽

Irvan Septrian Syah Putra Rasad ◽

Maylan Nimas ◽

Nadira Syabilla

Keyword(s):

Wound Healing ◽

Tooth Extraction ◽

Healing Process ◽

Inflammatory Cells ◽

Seed Extract ◽

Persea Americana ◽

Control Group ◽

Extraction Socket ◽

Significant Difference ◽

Socket Healing

Background: Inflammatory cells and fibroblasts have an essential role in the wound healing process. Persea americana Mill. seed categorises as a waste; it contains rich nutrients that can accelerate wound healing activity. Purpose: This study aims to determine the effect of Persea americana Mill. seed against inflammatory cells and fibroblast formation in tooth extraction socket healing. Methods: Ninety-six Sprague Dawley rats had their lower left molars removed. Forty-eight rats tested for inflammatory cells were divided into four groups: negative control group (IC1), positive control group (IC2), Persea americana Mill. seed extract concentrations of 50% (IE1) and 90% (IE2). Another 48 rats used for fibroblast were divided into three groups: the control group (FC1), Persea americana Mill. seed concentrations of 50% (FE1) and 90% (FE2). The gel was applied to the socket under general anaesthesia. Four rats from each group were decapitated for histopathological tissue preparations with Haematoxylin Eosin (HE) staining on the 3rd, 5th and 7th days for inflammatory cells and the 3rd, 5th, 7th and 14th days for fibroblast formation. The preparations for each research were scored under the microscope at 40x magnification. The obtained data was analysed using the Kruska—Wallis and the Mann—Whitney test. Results: A significant decrease (p<0.05) of inflammatory cells in IE2 on the 5th and 7th day. A significant increase (p<0.05) of fibroblast formation between treatment and control groups and no significant difference (p>0.05) between FE1 and FE2 was based on the interval days. Conclusion: Persea americana Mill. seed extract can decrease the inflammatory cells and accelerate the fibroblast formation in tooth extraction socket healing.

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Maresin 1 Promotes Wound Healing and Socket Bone Regeneration for Alveolar Ridge Preservation

Journal of Dental Research ◽

10.1177/0022034520917903 ◽

2020 ◽

Vol 99 (8) ◽

pp. 930-937 ◽

Cited By ~ 1

Author(s):

C.W. Wang ◽

S.H. Yu ◽

T. Fretwurst ◽

L. Larsson ◽

J.V. Sugai ◽

...

Keyword(s):

Wound Healing ◽

Postoperative Pain ◽

Tooth Extraction ◽

Alveolar Bone ◽

Lipid Mediator ◽

Preclinical Model ◽

Alveolar Ridge ◽

Extraction Socket ◽

Socket Healing ◽

Maresin 1

Tooth extraction results in alveolar bone resorption and is accompanied by postoperative swelling and pain. Maresin 1 (MaR1) is a proresolving lipid mediator produced by macrophages during the resolution phase of inflammation, bridging healing and tissue regeneration. The aim of this study was to examine the effects of MaR1 on tooth extraction socket wound healing in a preclinical rat model. The maxillary right first molars of Sprague-Dawley rats were extracted, and gelatin scaffolds were placed into the sockets with or without MaR1. Topical application was also given twice a week until complete socket wound closure up to 14 d. Immediate postoperative pain was assessed by 3 scores. Histology and microcomputed tomography were used to assess socket bone fill and alveolar ridge dimensional changes at selected dates. The assessments of coded specimens were performed by masked, calibrated examiners. Local application of MaR1 potently accelerated extraction socket healing. Macroscopic and histologic analysis revealed a reduced soft tissue wound opening and more rapid re-epithelialization with MaR1 delivery versus vehicle on socket healing. Under micro–computed tomography analysis, MaR1 (especially at 0.05 μg/μL) stimulated greater socket bone fill at day 10 as compared with the vehicle-treated animals, resulting in less buccal plate resorption and a wider alveolar ridge by day 21. Interestingly, an increased ratio of CD206+:CD68+ macrophages was identified in the sockets with MaR1 application under immunohistochemistry and immunofluorescence analysis. As compared with the vehicle therapy, local delivery of MaR1 reduced immediate postoperative surrogate pain score panels. In summary, MaR1 accelerated extraction wound healing, promoted socket bone fill, preserved alveolar ridge bone, and reduced postoperative pain in vivo with a rodent preclinical model. Local administration of MaR1 offers clinical potential to accelerate extraction socket wound healing for more predictable dental implant reconstruction.

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Effects of pre-extraction intermittent PTH administration on extraction socket healing in bisphosphonate administered ovariectomized rats

Scientific Reports ◽

10.1038/s41598-020-79787-w ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Jae-Young Kim ◽

Hyo-Won Jang ◽

Jung-In Kim ◽

In-Ho Cha

Keyword(s):

Tooth Extraction ◽

Bisphosphonate Therapy ◽

Ovariectomized Rats ◽

Control Group ◽

Micro Ct ◽

Micro Computed Tomography ◽

Extraction Socket ◽

Before And After ◽

Socket Healing ◽

Positive Effect

AbstractThe purpose of this study was to investigate the effect of administering intermittent parathyroid hormone (iPTH) before tooth extraction versus after tooth extraction on the risk of developing MRONJ in experimental animal model. Twenty-five ovariectomized rats received 6 weeks of bisphosphonate therapy. They were classified into 3 groups, based on the timing of the medication, as Control, Pre-PTH and Post-PTH groups. For Control group, normal saline was administered before and after tooth extraction. iPTH was administered during 4 weeks before tooth extraction for Pre-PTH group and after tooth extraction for Post-PTH group. The animals were euthanized 8 weeks after tooth extraction. Macroscopic, histological, micro-computed tomography (micro-CT), and histomorphometric examinations were conducted. The incidences of impaired healing were 11.11% both in Pre-PTH and Post-PTH groups, which was lower than the Control group (42.86%). Bone healing in the extraction socket, based on micro-CT and histomorphometry evaluations, was best in Post-PTH and worst in Control group. The Pre-PTH group showed moderate healing pattern. Despite of limitations in this study, the authors identified Pre-PTH group seems to have positive effect on extraction socket healing. With regard to timing, administering iPTH after tooth extraction was superior to applying it before tooth extraction.

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Efektivitas getah batang betadine (Jatropha multifida L.) terhadap penyembuhan luka pasca pencabutan gigi pada tikus Sprague-DawleyEffectiveness of betadine (Jatropha multifida L.) stem sap on the wound healing after tooth extraction in Sprague-Dawley rats

Jurnal Kedokteran Gigi Universitas Padjadjaran ◽

10.24198/jkg.v33i2.32563 ◽

2021 ◽

Vol 33 (2) ◽

pp. 145

Author(s):

Hendry Rusdy ◽

Astri Suryani Pasaribu Saruksuk ◽

Rahmi Syaflida Dalimunte ◽

Gostry Aldica Dohude

Keyword(s):

Wound Healing ◽

Tranexamic Acid ◽

Tooth Extraction ◽

Healing Process ◽

Control Group ◽

Wound Healing Process ◽

Sprague Dawley ◽

Sprague Dawley Rats ◽

Jatropha Multifida ◽

Plant Sap

Pendahuluan: Pencabutan gigi merupakan prosedur yang sering dilakukan di kedokteran gigi. Setelah pencabutan gigi akan dihasilkan suatu perlukaan. Proses penyembuhan luka dapat dipercepat pada kondisi tertentu. Salah satu bahan alami yang dapat membantu proses penyembuhan luka adalah getah tanaman betadine (Jatropha multifida L.). Penelitian bertujuan untuk menganalisis efektivitas getah tanaman betadine terhadap penyembuhan luka dan terhadap tanda-tanda infeksi pasca pencabutan gigi Metode: Desain penelitian studi eksperimental laboratorium dengan rancangan penelitian post-test only control group design menggunakan 30 ekor tikus Sprague-Dawley. Teknik pengambilan sampel yaitu convenience sampling. Sampel dibagi menjadi 2 kelompok yaitu kelompok perlakuan dan kelompok kontrol. Kelompok perlakuan diberikan getah tanaman betadine dan kelompok kontrol diberikan asam traneksamat secara oral menggunakan sonde lambung. Tunggu selama 4 jam setelah pemberian getah tanaman betadine dan asam traneksamat. Anestesi pada tikus menggunakan ketamin 50 mg/kg berat badan secara intramuskular kemudian dilakukan pencabutan pada gigi tikus. Pengamatan dilakukan dengan melihat kriteria klinis pada hari 1,3,7 dan diperhatikan sampai luka sembuh serta lihat tanda-tanda infeksi. Analisis data dilakukan dengan uji normalitasShapiro Wilik. Hasil penelitian menunjukkan bahwa data berdistribusi tidak normal. Analisis data dilanjutkan menggunakan uji statistik mann whitney. Hasil: Terdapat perbedaan signifikan penyembuhan luka soket pasca pencabutan gigi setelah diberikan getah betadine dan asam traneksamat dengan nilai p=0,037 (p<0,005). Simpulan: Pemberian getah tanaman betadine terbukti lebih efektif terhadap proses penyembuhan luka soket pasca pencabutan gigi dibandingkan dengan pemberian asam traneksamat. Kata kunci: tikus Sprague-Dawley; penyembuhan luka; pencabutan gigi; getah batang betadine ABSTRACTIntroduction: Tooth extraction is a procedure often performed in dentistry. Tooth extraction will always cause injuries. However, the wound healing process can be accelerated under certain conditions. One of the natural ingredients that can accelerate the wound healing process is betadine (Jatropha multifida L.) plant sap. The study was aimed to analyzed the effect of betadine plant sap on wound healing and signs of infection after tooth extraction. Methods: Experimental laboratory study design with post-test only control group design was conducted towards 30 Sprague-Dawley rats. The sampling technique was convenience sampling. The sample was divided into two groups, the treatment group and the control group. The treatment group was administered with betadine plant sap, and the control group was administered with tranexamic acid orally using a gastric probe, then waited 4 hours after. The anaesthesia was then performed using 50 mg/kg body weight of ketamine intramuscularly. The extraction was performed after. Observations was conducted at the clinical criteria on days 1, 3, and 7 and continue to be monitored until the wound heals. Then, the signs of infection were observed. Data analysis was carried out using the Shapiro Wilk normality test. The results showed that the data was not normally distributed. Thus, data analysis was continued using the Mann Whitney statistical test. Results: The results showed a significant difference in the healing of socket wounds after tooth extraction after being administered with betadine sap and tranexamic acid with a value of p=0.037 (p<0.005). Conclusions: Administration of betadine plant sap is proven to be more effective in accelerating the healing process of socket wounds after tooth extraction than tranexamic acid. Keywords: Sprague-Dawley rats; wound healing; pencabutan gigi; getah batang betadine

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