scholarly journals The effect of Persea americana Mill. seed extract on inflammatory cells and fibroblast formation in tooth extraction socket healing

2021 ◽  
Vol 54 (4) ◽  
pp. 190
Author(s):  
Yessy Ariesanti ◽  
Irvan Septrian Syah Putra Rasad ◽  
Maylan Nimas ◽  
Nadira Syabilla
Keyword(s):  
Wound Healing ◽  
Tooth Extraction ◽  
Healing Process ◽  
Inflammatory Cells ◽  
Seed Extract ◽  
Persea Americana ◽  
Control Group ◽  
Extraction Socket ◽  
Significant Difference ◽  
Socket Healing

Background: Inflammatory cells and fibroblasts have an essential role in the wound healing process. Persea americana Mill. seed categorises as a waste; it contains rich nutrients that can accelerate wound healing activity. Purpose: This study aims to determine the effect of Persea americana Mill. seed against inflammatory cells and fibroblast formation in tooth extraction socket healing. Methods: Ninety-six Sprague Dawley rats had their lower left molars removed. Forty-eight rats tested for inflammatory cells were divided into four groups: negative control group (IC1), positive control group (IC2), Persea americana Mill. seed extract concentrations of 50% (IE1) and 90% (IE2). Another 48 rats used for fibroblast were divided into three groups: the control group (FC1), Persea americana Mill. seed concentrations of 50% (FE1) and 90% (FE2). The gel was applied to the socket under general anaesthesia. Four rats from each group were decapitated for histopathological tissue preparations with Haematoxylin Eosin (HE) staining on the 3rd, 5th and 7th days for inflammatory cells and the 3rd, 5th, 7th and 14th days for fibroblast formation. The preparations for each research were scored under the microscope at 40x magnification. The obtained data was analysed using the Kruska—Wallis and the Mann—Whitney test. Results: A significant decrease (p<0.05) of inflammatory cells in IE2 on the 5th and 7th day. A significant increase (p<0.05) of fibroblast formation between treatment and control groups and no significant difference (p>0.05) between FE1 and FE2 was based on the interval days. Conclusion: Persea americana Mill. seed extract can decrease the inflammatory cells and accelerate the fibroblast formation in tooth extraction socket healing.

Method for quantitative assessment of surgical wound healing (for example, the hole of a removed tooth). Part I

2020 ◽  
Vol 25 (4) ◽  
pp. 349-356
Author(s):  
M. V. Lomakin ◽  
I. I. Soloshchanskii ◽  
A. A. Pokhabov ◽  
H. U. Bisultanov
Keyword(s):  
Wound Healing ◽  
Success Rate ◽  
Quantitative Assessment ◽  
Tooth Extraction ◽  
Healing Process ◽  
Real Life ◽  
Surgical Wound ◽  
Extraction Socket ◽  
Radiographic Findings ◽  
Socket Healing

Relevance. Surgical stages of the dental implant restoration are an indispensable part of the treatment procedures. However, underestimation of residual/ available alveolar bone volume after tooth extraction may challenge implantation planning. Socket healing process might depend on either a surgical technique, or local or systemic preexisting factors, that this study should present as quantitative data. Purpose is to develop a technique for quantitative assessment of a surgical wound healing, e.g. an extraction socket.Materials and methods. The study included 42 patients after extraction of different teeth during the preparatory procedures for further dental arch restoration with implants. Follow-ups, clinical data, laboratory and radiographic findings determined clinical, radiographic, and information values – dependent and independent variables as quantifiable clinical parameters – biomarkers; they were statistically analyzed.Results. The conducted analysis revealed correlation dependence between selected parameters. Highly significant interrelationship allowed calculating the overall success rate of wound/ socket healing after tooth extraction. A regression model was developed to explain 76% healing success rate.Conclusion. Thus, factors and conditions determining the healing of a surgical bone wound, e.g. an extraction socket, consistently affect each other. Numerical sequences of real-life processes characterize the extent and highly significant interrelations and allow predicting the mutual impact of the factors.

scholarly journals Application of pomegranate (Punica granatum Linn.) fruit extract for accelerating post-tooth extraction wound healing

2018 ◽  
Vol 51 (4) ◽  
pp. 189
Author(s):  
Intan Nirwana
Keyword(s):  
Wound Healing ◽  
Growth Factor ◽  
Treatment Group ◽  
Tooth Extraction ◽  
Transforming Growth Factor ◽  
Healing Process ◽  
Punica Granatum ◽  
Control Group ◽  
Wound Healing Process ◽  
Significant Difference

Background: Trauma occurring during tooth extraction can cause complications such as bleeding, infection, fracture and dry socket and constitutes an inflammatory response trigger. Pomegranate (Punica granatum Linn.) extract, which contains large amounts of punicallagin and ellagic acid, possesses various qualities, including; anti-inflammatory, anti-bacterial and anti-oxidant. Pomegranate extract can inhibit proinflammatory cytokine production, while also suppressing inflammation response thereby accelerating wound healing. Purpose: This study aimed to analyze the effect of pomegranate extract application to the tooth extraction wounds of Cavia cobaya (C. cobaya) on the expression of fibroblast growth factor-2 (FGF-2) and transforming growth factor β (TGF-β) on the fourth day of the wound-healing process. Methods: This study used 12 C. cobaya, divided into two groups, namely; control and treatment. The subjects were anesthetized, before their lower left central incisor was extracted and the entire socket filled with CMC-Na 3% in members of the control group and pomegranate extract in those of the treatment group. The twelve C. cobaya were sacrificed on day 4, their lower jaw subsequently being removed and decalcified for approximately 30 days. The mandibula tissue was stained using a immunohistochemical technique. FGF-2 and TGF-β were used to evaluate the healing process in the extracted tooth socket. Differences in the expression of FGF-2 and TGF-β were evaluated statistically by means of a t-test. Results: This study indicated a significant difference between the control and the treatment groups (p<0.05). The treatment group members whose sockets were filled with pomegranate extract showed high FGF-2 and TGF-β expression. Conclusion: This study confirmed that the administration of pomegranate extract to post-extraction tooth wounds of C. cobaya increases the expression of FGF-2 and TGF-β on day 4, thereby accelerating the wound healing process.

scholarly journals The effect of 3% binahong leaf extract gel on the wound healing process of post tooth extraction

2021 ◽  
Vol 54 (2) ◽  
pp. 57
Author(s):  
Olivia Avriyanti Hanafiah ◽  
Diana Sofia Hanafiah ◽  
Rahmi Syaflida
Keyword(s):  
Wound Healing ◽  
Tooth Extraction ◽  
Leaf Extract ◽  
Healing Process ◽  
Control Group ◽  
Wound Healing Process ◽  
Herbaceous Plant ◽  
Significant Difference ◽  
Analyse Data ◽  
And Control

Background: One of the common frequently performed procedures in dentistry is tooth extraction. After tooth extraction, a series of biological events occurs in the alveolar socket that ultimately results in healing of the socket. Binahong (Anredera cordifolia) is an herbaceous plant that contains active compounds that may accelerate the wound healing process. Purpose: The aim of this study is to investigate the effect of 3% binahong leaf extract gel on the healing process of the postextraction wound. Methods: 3% binahong leaf extract gel was prepared with the composition of 1 g carbopol, 1 g hydroxypropilmethylcellulose (HPMC), 4 g glycerin, 3 g triethanolamine (TEA), 0.04 g Nipagin, 0.04 g Nipasol, 1.2 g binahong leaf extract and Aq ad 40 g. A total of 18 people were subjected to tooth extraction and were then divided into two groups, with nine people in the binahong group and nine in the control group. Following that, residual socket volume (RSV) was examined on days 3, 7 and 14 after the extraction in both the binahong and control groups. The RSV value was obtained by calculating the mesiodistal x bucolingual width x the depth probing and measured using a calliper and probe. Repeated analysis of variance (ANOVA) and Friedman test followed by Wilcoxon and dependent t-test (p<0.05) were used to analyse data. Results: Significant difference in the mean RSV between the control and binahong groups was observed on days 3, 7 and 14 post tooth extraction. The RSV value in the binahong group was lower when compared to the control group (p <0.05). Conclusion: 3% binahong leaf extract gel can accelerate the process of healing socket wounds.

scholarly journals The possibility of polymorphonuclear leukocyte activation in dental socket healing by freeze-dried Aloe vera induction

2021 ◽  
Vol 54 (3) ◽  
pp. 124
Author(s):  
Pratiwi Soesilawati ◽  
Ester Arijani Rachmat ◽  
Ira Arundina ◽  
Nita Naomi
Keyword(s):  
Wound Healing ◽  
Tooth Extraction ◽  
Healing Process ◽  
Aloe Vera ◽  
Inflammatory Cells ◽  
Control Group ◽  
Wound Healing Process ◽  
Control Group Design ◽  
Aloe Vera Gel ◽  
Freeze Dried

Background: Research has proved that Aloe vera has anti-inflammatory, anti-bacterial, and immunomodulator properties that can accelerate the healing process. These properties could be beneficial in the tooth extraction wound-healing process. Neutrophils are the first inflammatory cells to invade and are the predominant cell markers in the wound for 24 hours after injury. Polymorphonuclear leukocytes (PMN) provide initial protection against micro-organisms, as they engulf and digest foreign bodies. Purpose: The aim of this study was to prove the effect of Aloe vera 90% gel application on the PMN count in the healing process of Cavia cobaya following tooth extraction. Methods: This laboratory experimental research was done using Post-Test Only Control Group Design. The lower incisive of twelve male Cavia cobayas were extracted then divided into four groups. Group P1 and P3 had 90% Aloe vera gel applied into the socket and sutured; group K1 and K3, received no 90% Aloe vera gel application and acted as controls. Samples from group P1 and K1 were terminated and had their mandibula taken one day after tooth extraction, and those from group P3 and K3 were terminated after three days. Tissues were then processed into histology slides, and PMN cells were counted. Results: There were significant differences in the PMN count between groups K1, P1, and K3 compared with P3 (p < 0.05). Higher PMN count was shown on the group containing Aloe vera 90% gel compared with control group, both on day one and three after tooth extraction. Conclusion: Aloe vera 90% gel can increase the PMN count in the inflammation phase of the tooth extraction wound-healing process.

scholarly journals Effects of pre-extraction intermittent PTH administration on extraction socket healing in bisphosphonate administered ovariectomized rats

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Jae-Young Kim ◽  
Hyo-Won Jang ◽  
Jung-In Kim ◽  
In-Ho Cha
Keyword(s):  
Tooth Extraction ◽  
Bisphosphonate Therapy ◽  
Ovariectomized Rats ◽  
Control Group ◽  
Micro Ct ◽  
Micro Computed Tomography ◽  
Extraction Socket ◽  
Before And After ◽  
Socket Healing ◽  
Positive Effect

AbstractThe purpose of this study was to investigate the effect of administering intermittent parathyroid hormone (iPTH) before tooth extraction versus after tooth extraction on the risk of developing MRONJ in experimental animal model. Twenty-five ovariectomized rats received 6 weeks of bisphosphonate therapy. They were classified into 3 groups, based on the timing of the medication, as Control, Pre-PTH and Post-PTH groups. For Control group, normal saline was administered before and after tooth extraction. iPTH was administered during 4 weeks before tooth extraction for Pre-PTH group and after tooth extraction for Post-PTH group. The animals were euthanized 8 weeks after tooth extraction. Macroscopic, histological, micro-computed tomography (micro-CT), and histomorphometric examinations were conducted. The incidences of impaired healing were 11.11% both in Pre-PTH and Post-PTH groups, which was lower than the Control group (42.86%). Bone healing in the extraction socket, based on micro-CT and histomorphometry evaluations, was best in Post-PTH and worst in Control group. The Pre-PTH group showed moderate healing pattern. Despite of limitations in this study, the authors identified Pre-PTH group seems to have positive effect on extraction socket healing. With regard to timing, administering iPTH after tooth extraction was superior to applying it before tooth extraction.

673 Effect of Subcutaneous Topical Ozone Therapy on Second Degree Burn Wounds in Rats: An Experimental Study

2021 ◽  
Vol 42 (Supplement_1) ◽  
pp. S193-S193
Author(s):  
Emre Karakaya ◽  
Aydincan Akdur ◽  
H Ebru ◽  
Ayvazoglu Soy ◽  
Alev Ok Atilgan ◽  
...  
Keyword(s):  
Wound Healing ◽  
Healing Process ◽  
Silver Sulfadiazine ◽  
Male Rats ◽  
Control Group ◽  
Ozone Therapy ◽  
Burn Wounds ◽  
Degree Burn ◽  
Significant Difference ◽  
Second Degree

Abstract Introduction Burn is one of the most severe traumas that causes coagulative destruction of the skin. The use of various products that accelerate wound healing in patients with burn may affect the patient’s survival and reduce the complications that may be seen. In the present study we aimed effects of subcutaneous ozone injection on second degree burn wound. Methods A total of 72 Sprague-Dawley male rats included in the study were divided randomly into three groups (control group (CG), silver sulfadiazine group (SG), ozone group (OG)) and each group was divided randomly two subgroups (as sacrificed on d7 and on d14).A deep second degree scald burns were created on the lower back. In CG subcutaneous 0.9% serum saline was injected daily into the burn area. In SG, burns were dressed with silver sulfadiazine daily and in OG subcutaneous ozone was injected daily into the burn area. Tissue hydroxyproline level measurement and histopathological evaluation were done. Results When the groups were compared in terms of weight change, no significant difference was found on the 7th and 14th days. In the evaluation made in terms of tissue hydroxyproline, tissue hydroxyproline level in OG was found to be significantly higher on both the 7th and 14th days (p &lt; 0.001). In histopathological evaluations, it was determined that wound healing in OG was significantly higher than in the other groups. Conclusions According to the results, subcutaneous ozone therapy is more effective than silver sulphadiazine in the healing process of second-degree burn wounds and it can be safely used in the treatment of burn wounds.

scholarly journals PENGARUH PEMBERIAN GEL KOMBINASI EKSTRAK GETAH PEPAYA (Carica papaya L) DAN EKSTRAK DAGING LIDAH BUAYA (Aloe vera) TERHADAP PROSES PENYEMBUHAN ULKUS TRAUMATIKUS PADA MALE WISTAR RATS YANG MENDERITA DIABETES MELITUS

2015 ◽  
Vol 2 (1) ◽  
pp. 41
Author(s):  
Umi Kulsum ◽  
Ratnawati Hendari ◽  
Siti Chumaeroh
Keyword(s):  
Wound Healing ◽  
Ulcer Healing ◽  
Wistar Rats ◽  
Povidone Iodine ◽  
Healing Process ◽  
Aloe Vera ◽  
Control Group ◽  
Significant Difference ◽  
Male Wistar Rats ◽  
Papaya Latex

Introduction: Research on the use of papaya and aloevera sap on wound healing each been done, but effects the combination of the two extracts on wound healing in diabetes mellitus condition has not been done.Purpose: This study aims to determine the effect of a gel combination of papaya latex and aloevera flesh extracts on the traumatics ulcer healing process in diabetic rats.Methods: True experimental laboratory research with the post-test-only control group design that performed on 28 male wistar rats which were divided intofour groups: DM and non-DM rat that treatment with povidone iodine and gel combination of papaya latex and aloevera flesh extract. The treatment was done for 7 days with smearing that was given 2x/day. Traumatics ulcer healing seen from the number of fibroblasts that then were analyzed by One WayAnova and post hoc LSD test.Results: The results showed the number of fibroblasts in DM and non-DM rat with povidone iodine were 44.6 and 42.73; in the DM and non-DM rat with a gel combination of papaya latex and aloe vera flesh extracts were 61.10 and 77.03. One Way Anova test resulted p-value = 0.000, it was mean that there were at least a significant difference on the number of fibroblasts in the two groups. Differences of the number fibroblasts was shown in all groups, except in the group DM and non-DM rats with povidone iodine (p = 0.764).Conclusion: Conclusion of the study was the provision of a gel combination of papaya latex and aloevera flesh extracts effect on the traumatics ulcer healing process in diabetic rats.

scholarly journals PENGARUH APLIKASI VIRGIN COCONUT OIL TERHADAP PENINGKATAN JUMLAH FIBROBLAS PADA LUKA PASCA PENCABUTAN GIGI PADA RATTUS NOVERGICUS

2015 ◽  
Vol 1 (2) ◽  
pp. 29
Author(s):  
Anggun Hibah Jannah Tamara ◽  
Yayun Siti Rochmah ◽  
Rochman Mujayanto
Keyword(s):  
Wound Healing ◽  
Healing Process ◽  
Health Benefit ◽  
Coconut Oil ◽  
Control Group ◽  
Virgin Coconut Oil ◽  
Complex Biological Process ◽  
Significant Difference ◽  
Per Oral ◽  
Levene Test

Background: Wound healing is a complex biological process. Fibroblast is one of cell which is responsible for the wound healing process. Virgin Coconut Oil (VCO) have health benefit as antibacterial, antiinflamatory, antioxidant, and immunomodulatory. This research was purposed to know the influence use of VCO over the amount of fibroblast in wound healing after dental extraction.Method: Research quasi experimental with post test control group design and using 15 Rattus novergicus which were divided into three groups (control positive, VCO topical application, and VCO per oral application). Tooth removal was committed by using explorer, scalpel-blade, and arterial clamp. Then VCO applied everyday for 7 days. Day 7th Rattus novergicus were died, the socket after extraction were made into histological form, then the amount of fibroblast were counted with 5 field of views and made in average. Result: The number of fibroblast of the most numerous was VCO per oral ( 48,6 ± 49,8 ), VCO topical ( 36,2 ± 38,8 ), and control positive ( 33,4 ± 35,2 ). The results of the Saphiro Wilk test and Levene test acquired data normally distributed and homogeny, so it continued to One Way Anova and data showed there were significant difference among the groups of samples with value p<0,05.Conclusion: The conclusion of this research is VCO could increase the number of fibroblasts in wound healing after dental extractions

scholarly journals Evaluation of effect of local exogenous application of Myrrh oil on healing of wound incisions of facial skin (Histochemical, Histological and Histomorphometrical study in rabbits)

2019 ◽  
Vol 31 (4) ◽  
Author(s):  
Nawar Bahjet Kamil ◽  
Nada M. Al-Ghaban
Keyword(s):  
Healing Process ◽  
Inflammatory Cells ◽  
Control Group ◽  
Facial Skin ◽  
Histological Findings ◽  
Control Groups ◽  
Mean Values ◽  
Significant Difference ◽  
The Right ◽  
New Zealand Rabbits

Aim of the study: Is to evaluate the effect of myrrh oil local application on the healing process of skin wounds histologically , histomorphometrically and , histochemically. Materials and methods:Twenty male white New Zealand rabbits were used in this study. An incisional wounds with full thickness depth and of 2 cm length were done on both sides of the cheek skin of each rabbit. The left sided incisions (the control group) were irrigated with distilled water (10µL). The right sided incisions (the experimental groups) were treated with myrrh oil (10µL). Each group was subdivided into 4 subgroups according to the healing interval into 1,3,7 and 14 days(5 rabbits for each group). Results: Histological findings of our current study showed a highly significant difference between the experimental and the control groups in count of the inflammatory cells which showed that mean values increased with time for the control and the experimental groups. The histomorphometrical findings had shown that the thickness of the epithelium was nearly completed at about 7 days for the experimental groups and at about 14 days for the control. The blood vessels count was recorded to have a highly significant difference between the groups at days 1 and 3 only. The histochemical findings had shown that the collagen fibers remodeling had recorded a highly significant difference between the control and the experimental groups at days 7 and 14. Conclusion: The current study had revealed that myrrh oil accelerates wounds healing in rabbits. Key word:Wound healing , Myrrh oil, Masson’s Trichrome staining.

scholarly journals Sufficient therapeutic effect of cryopreserved frozen adipose-derived regenerative cells on burn wounds

2018 ◽  
Author(s):  
Yasuhiko Kaita ◽  
Takehiko Tarui ◽  
Hideaki Yoshino ◽  
Takeaki Matsuda ◽  
Yoshihiro Yamaguchi ◽  
...  
Keyword(s):  
Wound Healing ◽  
Therapeutic Effect ◽  
Healing Process ◽  
Control Group ◽  
Type I ◽  
Burn Wound ◽  
Burn Wounds ◽  
Burn Wound Healing ◽  
Significant Difference ◽  
Regenerative Cells

AbstractThe purpose of this study was to evaluate whether cryopreserved (frozen) adipose-derived regenerative cells (ADRCs) have a therapeutic effect on burn wound healing as well as freshly isolated (fresh) ADRCs.Full thickness burns were created on dorsum of nude mice and burn wound was excised. The wound was covered by artificial dermis with; (i) fresh ADRCs, (ii) frozen ADRCs, and (iii) PBS (control). The assessment for wound healing was performed by morphological, histopathological and immunohistochemical analyses.In vivo analyses exhibited the significant therapeutic effect of frozen ADRCs on burn wound healing up to the similar or higher level of fresh ADRCs. There were significant differences of wound closure, epithelized tissue thickness, and neovascularization between the treatment groups and control group. Although there was no significant difference of therapeutic efficacy between fresh ADRC group and frozen ADRC group, frozen ADRCs improved burn wound healing process in dermal regeneration with increased great type I collagen synthesis compared with fresh ADRCs.These findings indicate that frozen ADRCs allow us to apply not only quickly but also for multiple times, and the cryopreserved ADRCs could therefore be useful for the treatment of burn wounds in clinical settings.

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