Effects of pre-extraction intermittent PTH administration on extraction socket healing in bisphosphonate administered ovariectomized rats

AbstractThe purpose of this study was to investigate the effect of administering intermittent parathyroid hormone (iPTH) before tooth extraction versus after tooth extraction on the risk of developing MRONJ in experimental animal model. Twenty-five ovariectomized rats received 6 weeks of bisphosphonate therapy. They were classified into 3 groups, based on the timing of the medication, as Control, Pre-PTH and Post-PTH groups. For Control group, normal saline was administered before and after tooth extraction. iPTH was administered during 4 weeks before tooth extraction for Pre-PTH group and after tooth extraction for Post-PTH group. The animals were euthanized 8 weeks after tooth extraction. Macroscopic, histological, micro-computed tomography (micro-CT), and histomorphometric examinations were conducted. The incidences of impaired healing were 11.11% both in Pre-PTH and Post-PTH groups, which was lower than the Control group (42.86%). Bone healing in the extraction socket, based on micro-CT and histomorphometry evaluations, was best in Post-PTH and worst in Control group. The Pre-PTH group showed moderate healing pattern. Despite of limitations in this study, the authors identified Pre-PTH group seems to have positive effect on extraction socket healing. With regard to timing, administering iPTH after tooth extraction was superior to applying it before tooth extraction.

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On the Causality Between Dentinal Defects and Root Canal Preparation: A Micro-CT Assessment

Brazilian Dental Journal ◽

10.1590/0103-6440201601002 ◽

2016 ◽

Vol 27 (6) ◽

pp. 664-669 ◽

Cited By ~ 20

Author(s):

Gustavo De-Deus ◽

Felipe Gonçalves Belladonna ◽

Juliana Roter Marins ◽

Emmanuel João Nogueira Leal Silva ◽

Aline de Almeida Neves ◽

...

Keyword(s):

Cross Section ◽

Root Canal ◽

Optical Microscope ◽

Control Group ◽

Micro Ct ◽

Micro Computed Tomography ◽

Root Canal Preparation ◽

Micro Cracks ◽

Before And After ◽

Experimental Group

Abstract This study aimed to evaluate the cause-effect relationship between canal preparation with ProTaper Universal (PTU) system and dentinal defects formation using micro-computed tomography (micro-CT) analysis. Forty mesial canals of mandibular molars with a type II Vertucci's canal configuration were scanned at an isotropic resolution of 14.16 µm. The sample was assigned to an experimental (n = 30) and a control (n = 10) groups, and the mesial canals were prepared with PTU system up to F2 instrument. The specimens from the experimental group were scanned and the cross-section images of the mesial roots, before and after preparation, were screened to identify the presence of dentinal defects. In the control group, the specimens were sectioned perpendicularly to the long axis of the root into 1-mm-thick slices (n = 80) and examined under optical microscope. Once a dentinal defect was detected, the slice was scanned through micro-CT. In the experimental group, dentinal micro-cracks were observed in 4,828 slices (24.04%). In all cross-section images, dentinal defects identified in the postoperative images were already present in the corresponding preoperative image. In the control group, 13 out of 80 slices (16.25%) had at least one dentinal defect visualized under stereomicroscopy, which was identified after a further micro-CT scanning. Micro-CT showed reliability as similar as optical microscopy in detecting dentinal defects, adding the possibility of tracking the dentinal tissue, before and after canal preparation, and providing a clear visualization of micro-cracks. Root canal preparation with PTU system did not induce the formation of new dentinal defects.

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The effect of Persea americana Mill. seed extract on inflammatory cells and fibroblast formation in tooth extraction socket healing

Dental Journal (Majalah Kedokteran Gigi) ◽

10.20473/j.djmkg.v54.i4.p190-194 ◽

2021 ◽

Vol 54 (4) ◽

pp. 190

Author(s):

Yessy Ariesanti ◽

Irvan Septrian Syah Putra Rasad ◽

Maylan Nimas ◽

Nadira Syabilla

Keyword(s):

Wound Healing ◽

Tooth Extraction ◽

Healing Process ◽

Inflammatory Cells ◽

Seed Extract ◽

Persea Americana ◽

Control Group ◽

Extraction Socket ◽

Significant Difference ◽

Socket Healing

Background: Inflammatory cells and fibroblasts have an essential role in the wound healing process. Persea americana Mill. seed categorises as a waste; it contains rich nutrients that can accelerate wound healing activity. Purpose: This study aims to determine the effect of Persea americana Mill. seed against inflammatory cells and fibroblast formation in tooth extraction socket healing. Methods: Ninety-six Sprague Dawley rats had their lower left molars removed. Forty-eight rats tested for inflammatory cells were divided into four groups: negative control group (IC1), positive control group (IC2), Persea americana Mill. seed extract concentrations of 50% (IE1) and 90% (IE2). Another 48 rats used for fibroblast were divided into three groups: the control group (FC1), Persea americana Mill. seed concentrations of 50% (FE1) and 90% (FE2). The gel was applied to the socket under general anaesthesia. Four rats from each group were decapitated for histopathological tissue preparations with Haematoxylin Eosin (HE) staining on the 3rd, 5th and 7th days for inflammatory cells and the 3rd, 5th, 7th and 14th days for fibroblast formation. The preparations for each research were scored under the microscope at 40x magnification. The obtained data was analysed using the Kruska—Wallis and the Mann—Whitney test. Results: A significant decrease (p<0.05) of inflammatory cells in IE2 on the 5th and 7th day. A significant increase (p<0.05) of fibroblast formation between treatment and control groups and no significant difference (p>0.05) between FE1 and FE2 was based on the interval days. Conclusion: Persea americana Mill. seed extract can decrease the inflammatory cells and accelerate the fibroblast formation in tooth extraction socket healing.

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Influence on Forgiveness, Character Strengths and Satisfaction with Life of a Short Mindfulness Intervention via a Spanish Smartphone Application

International Journal of Environmental Research and Public Health ◽

10.3390/ijerph18020802 ◽

2021 ◽

Vol 18 (2) ◽

pp. 802

Author(s):

Juan Pablo Pizarro-Ruiz ◽

Nuria Ordóñez-Camblor ◽

Mario Del-Líbano ◽

María-Camino Escolar-LLamazares

Keyword(s):

Negative Affect ◽

Satisfaction With Life ◽

Character Strengths ◽

Well Being ◽

Smartphone Application ◽

Control Group ◽

Psychological Practice ◽

Before And After ◽

Reducing Stress ◽

Positive Effect

Mindfulness-based interventions (MBI) are a recognized effective psychological practice characterized by attention control, awareness, acceptance, non-reactivity, and non-judgmental thinking obtained through the practice of meditation. They have been shown to be useful in reducing stress and enhancing well-being in different contexts. In this research, the effectiveness of an MBI was evaluated on variables that can promote successful job performance such as mindfulness trait, positive and negative affect, forgiveness, personality strengths and satisfaction with life. The intervention was carried out through a smartphone application called “Aire Fresco” (Fresh Air) during 14 days in the middle of the quarantine produced by the Covid-19 pandemic. The study sample was composed of 164 Spanish people who were distributed in two groups: control group and experimental group, which were evaluated before and after the intervention. The MANCOVA performed showed an overall positive effect of the intervention on the variables evaluated. The different ANCOVAs carried out showed that the intervention was beneficial in increasing mindfulness trait, reducing negative affect or increasing life satisfaction, among others. Our study is, as far as we know, the first to demonstrate the effectiveness of a brief intervention in mindfulness conducted using a smartphone application in Spanish.

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The Effect of Memory Strategy Training on Vocabulary Learning by EFL Female Students of the College of Languages and Translation: An Experimental Study

10.31235/osf.io/5vhrq ◽

2018 ◽

Author(s):

Arab World English Journal ◽

Ghadah Fahad Alzaidi

Keyword(s):

Traditional Instruction ◽

Strategy Training ◽

Control Group ◽

King Saud University ◽

New Words ◽

Before And After ◽

Post Test ◽

Students Attitudes ◽

Positive Effect ◽

Experimental Group

The purpose of this study was to investigate the effectiveness of memory strategies (MSs)in improving students' ability to recall and spell newly learned vocabulary items. The researcheronly considered three types of MSs: grouping, placing new words in to a context and structuredreviewing. In addition, the study used a questionnaire that explored students' attitudes towardsMSs in general, and towards the specific MSs that they have been trained to use, as well as therelationship between MSs and motivation and between MSs and anxiety. intact groups (63 students in total) were selected from level three of the College of Languages and Translation (COLT) at King Saud University (KSU), Kingdom of Saudi Arabia. The two groups were assigned to the experimental group (36 students) and the control group(27students). The control group received traditional instruction that depended on the textbook without any MS training, whereas the experimental group received a combination of traditional instruction and MS training. A pre- and post-test were administered to both groups before and after the experiment to examine students' ability to recall the meaning of vocabulary items and their ability to spell them correctly. The experiment lasted for fourteen weeks through which the participants received seven MS training sessions. The study found out that MS training was effective in improving students' ability to recall the meaning of vocabulary items when needed and their ability to spell them correctly. Moreover, the results showed that the subjects of the experimental group showed positive attitude towards MSs and most of them agreed on the positive effect that MSs had on raising their motivation and reducing their anxiety while learning vocabulary.

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The 2nd Baltic Osseointegration Academy and Lithuanian University of Health Sciences Consensus Conference 2019. Summary and Consensus Statements: Group I - Biological Aspects of Tooth Extraction, Socket Healing and Indications for Socket Preservation

Journal of Oral and Maxillofacial Research ◽

10.5037/jomr.2019.10304 ◽

2019 ◽

Vol 10 (3) ◽

Author(s):

Gintaras Juodzbalys ◽

Povilas Daugela ◽

Onurcem Duruel ◽

Maria Helena Fernandes ◽

Pedro de Sousa Gomes ◽

...

Keyword(s):

Tooth Extraction ◽

Health Sciences ◽

Consensus Conference ◽

Extraction Socket ◽

Socket Preservation ◽

Group I ◽

Consensus Statements ◽

Socket Healing ◽

Biological Aspects

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Changes in PGE2 signaling after submandibulectomy alter post-tooth extraction socket healing

Wound Repair and Regeneration ◽

10.1111/wrr.12625 ◽

2018 ◽

Vol 26 (2) ◽

pp. 153-162 ◽

Cited By ~ 3

Author(s):

Claudia Ester Mohn ◽

Gastón Rodolfo Troncoso ◽

Clarisa Bozzini ◽

María Inés Conti ◽

Javier Fernandez Solari ◽

...

Keyword(s):

Tooth Extraction ◽

Extraction Socket ◽

Socket Healing

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BMP-2/β-TCP Local Delivery for Bone Regeneration in MRONJ-Like Mouse Model

International Journal of Molecular Sciences ◽

10.3390/ijms21197028 ◽

2020 ◽

Vol 21 (19) ◽

pp. 7028

Author(s):

Akihiro Mikai ◽

Mitsuaki Ono ◽

Ikue Tosa ◽

Ha Thi Thu Nguyen ◽

Emilio Satoshi Hara ◽

...

Keyword(s):

Bone Formation ◽

Bone Remodeling ◽

Tooth Extraction ◽

Pathological Condition ◽

Immunohistochemical Analysis ◽

Osteonecrosis Of The Jaw ◽

Control Group ◽

Extraction Socket ◽

Osteoclast Activity ◽

Prevention Studies

Medication-related osteonecrosis of the jaw (MRONJ) is a severe pathological condition associated mainly with the long-term administration of bone resorption inhibitors, which are known to induce suppression of osteoclast activity and bone remodeling. Bone Morphogenetic Protein (BMP)-2 is known to be a strong inducer of bone remodeling, by directly regulating osteoblast differentiation and osteoclast activity. This study aimed to evaluate the effects of BMP-2 adsorbed onto beta-tricalcium phosphate (β-TCP), which is an osteoinductive bioceramic material and allows space retention, on the prevention and treatment of MRONJ in mice. Tooth extraction was performed after 3 weeks of zoledronate (ZA) and cyclophosphamide (CY) administration. For prevention studies, BMP-2/β-TCP was transplanted immediately after tooth extraction, and the mice were administered ZA and CY for an additional 4 weeks. The results showed that while the tooth extraction socket was mainly filled with a sparse tissue in the control group, bone formation was observed at the apex of the tooth extraction socket and was filled with a dense connective tissue rich in cellular components in the BMP-2/β-TCP transplanted group. For treatment studies, BMP-2/β-TCP was transplanted 2 weeks after tooth extraction, and bone formation was followed up for the subsequent 4 weeks under ZA and CY suspension. The results showed that although the tooth extraction socket was mainly filled with soft tissue in the control group, transplantation of BMP-2/β-TCP could significantly accelerate bone formation, as shown by immunohistochemical analysis for osteopontin, and reduce the bone necrosis in tooth extraction sockets. These data suggest that the combination of BMP-2/β-TCP could become a suitable therapy for the management of MRONJ.

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Comparative Analysis of Blood Clot, Plasma Rich in Growth Factors and Platelet-Rich Fibrin Resistance to Bacteria-Induced Fibrinolysis

Microorganisms ◽

10.3390/microorganisms7090328 ◽

2019 ◽

Vol 7 (9) ◽

pp. 328 ◽

Cited By ~ 1

Author(s):

Tomas Puidokas ◽

Mantas Kubilius ◽

Donatas Nomeika ◽

Gintaras Januzis ◽

Erika Skrodeniene

Keyword(s):

Blood Clot ◽

Study Groups ◽

Control Group ◽

Platelet Concentrates ◽

Extraction Socket ◽

Human Volunteers ◽

Alveolar Osteitis ◽

Active Bacterium ◽

Positive Effect ◽

D Dimer

Alveolar osteitis (AO) is a common, painful postoperative complication after tooth extraction. Fibrinolytic activity in the extraction socket is one etiological factor. Platelet concentrates are used to prevent and treat AO. The aim of this study was to find out whether the positive effect of platelet concentrates can be related to resistance to bacteria-induced fibrinolysis. Blood from 45 human volunteers was used to prepare four media: blood clot medium as control group; PRF and PRGF first fraction (PRGF I) and PRGF second fraction (PRGF II) as study groups. Additionally, collected blood was used for blood plasma preparation on which evaluation of initial value of d-dimer concentration was performed. A solution of five different microbes (Staphylococcus aureus, Streptococcus pyogenes, Streptococcus pneumonia, Bacillus cereus, and Candida albicans) was adjusted to 0.5 McFarland (1 × 108 CFU/mL) and then diluted to 0.25 McFarland (0.5 × 108 CFU/mL). The d-dimer concentration was evaluated after one and three hours of bacteria exposure. The resistance to fibrinolysis was not statistically distinguished among any media groups at any time. S. pneumoniae was statistically active in PRF after three hours. C. albicans was statistically active in PRGF II after one hour and in PRF between the first and third hour and after three hours. S. aureus and B. cereus were statistically active in PRGF II after three hours. S. pyogenes was statistically active after one hour, between the first and third hour, and after the third hour in all groups. S. pyogenes was the most active bacterium. Different blood formulations were not distinguishable based on resistance to bacteria-induced fibrinolysis. Low fibrinolytic properties of the found major microbes suggests that bacteria-induced fibrinolysis is one of the leading causes of absence of a clot in a post-extraction socket to be clinically insignificant. The initial absence of a clot or its mechanical elimination during formation or the healing period are major causes of dry socket.

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