scholarly journals Selection of the optimal culture medium for cultivation Fusarium sporotrichioides Sherb

2020 ◽  
Author(s):  
I.L. Astapchuk ◽  
◽  
N.A. Marchenko ◽  
G.V. Yakuba ◽  
A.I. Nasonov ◽  
...  
Keyword(s):  
Growth Rate ◽  
Growth And Development ◽  
Maximum Degree ◽  
Culture Medium ◽  
Culture Media ◽  
High Variability ◽  
Fusarium Sporotrichioides ◽  
Cultural Characteristics ◽  
Colony Diameter ◽  
Selection Of

The influence of various culture media on the growth, morphological and cultural characteristics of the fungus F. sporotrichioides was studied. Ten culture media were used in our research. A comparative study of the growth rate of the F. sporotrichioides mycelium made it possible to identify two media that are the most suitable for the cultivation and identification of this species, namely carrot and tomato agar. We took into account such criteria as ensuring the maximum degree of sporulation, rapid growth and development of mycelium (the 7th day), colony diameter (71–78 mm), as well as the ease of preparation. Nirenberg culture medium can be used to obtain a large number of conidia of the fungus. Because of the high variability of cultural characteristics of F. sporotrichioides, we recommend using different composition of media.

scholarly journals Selection of filamentous fungi that are resistant to the herbicides atrazine, glyphosate and pendimethalin

2021 ◽  
Vol 43 ◽  
pp. e51656
Author(s):  
Nara Priscila Barbosa Bravim ◽  
Anatércia Ferreira Alves ◽  
José Fábio França Orlanda ◽  
Patricia Barbosa Rodrigues Silva
Keyword(s):  
Filamentous Fungi ◽  
Mycelial Growth ◽  
Culture Medium ◽  
Agricultural Soils ◽  
Culture Media ◽  
Fusarium Verticillioides ◽  
Fungal Growth ◽  
Relative Growth ◽  
Maximum Inhibition ◽  
Selection Of

The objective of the present study was to isolate fungi from agricultural soils and evaluate fungal growth in culture medium contaminated with atrazine, glyphosate and pendimethalin. Filamentous fungi were isolated from agricultural soils and cultured in a modified culture medium containing 0, 10, 20, 50, and 100 μg mL-1 atrazine, glyphosate and pendimethalin for 14 days at 28°C. The fungi that presented optimal and satisfactory growth were plated in Sabouraud culture medium with 4% dextrose and containing the herbicides at concentrations of 0, 10, 20, 50, and 100 μg mL-1 for seven days at 28°C. The mean mycelial growth values were submitted to analysis of variance and the Tukey test (p < 0.05%) for comparison and relative growth determination, and maximum inhibition rates were calculated. The isolated fungi Aspergillus fumigatus, Fusarium verticillioides and Penicillium citrinum were shown to be resistant to atrazine, glyphosate and pendimethalin. F. verticillioides showed higher mean mycelial growth in the culture media contaminated with atrazine and glyphosate than the other two fungi. In the culture medium contaminated with pendimethalin, F. verticillioides, and A. fumigatus presented the highest mean mycelial growth values.

scholarly journals Spirulina Culture in Bangladesh XI: Selection of a Culture Medium, Suitable for Culturing a Local Strain of Spirulina

1970 ◽  
Vol 41 (3) ◽  
pp. 227-234
Author(s):  
R Khatun ◽  
P Noor ◽  
N Akhter ◽  
MAA Jahan ◽  
M Hossain ◽  
...  
Keyword(s):  
Spirulina Platensis ◽  
Climatic Condition ◽  
Large Scale ◽  
Culture Medium ◽  
Culture Media ◽  
Local Strain ◽  
Condition Effect ◽  
Selection Of

Large scale Spirulina culture is possible in Bangladesh inspite of unpredictable climatic condition. Effect of different culture media on the growth of a local strain of Spirulina (Spirulina platensis) was studied. Bangladesh medium (Bd1) was found to be more favourable for the growth of the alga. All together three culture media were included in this study. Bangladesh J. Sci. Ind. Res. 41(3-4), 227-234, 2006

Optimization of Culture Media and ITS Identification of Tricholoma matsutake

2013 ◽  
Vol 634-638 ◽  
pp. 1574-1580
Author(s):  
Ji Zhong Wang ◽  
Zhong Hai Jiang ◽  
Shang Yong Zheng
Keyword(s):  
Growth Rate ◽  
Culture Medium ◽  
Culture Media ◽  
Fruit Body ◽  
Tricholoma Matsutake ◽  
Its Sequence ◽  
Average Growth Rate ◽  
Average Growth ◽  
Rdna Its ◽  
Rdna Its Sequences

Aims: To optimize the culture media of 0288 Tricholoma matsutake and to identify it according to its rDNA ITS sequence. Methods: Fruit body, collected from Heilongjiang province, is used as the material, and the mycelium is isolated and radiated under ultraviolet. Then the radiated mycelium is cultured in five different media and its morphology of fungus colony and growth rate are investigated. After that, the rDNA ITS sequences of 0288 and the natural fruit body of T. matsutake are analyzed and identified by PCR and sequencing. Results: The radiated mycelium grew faster in formula A culture medium than others and its average growth rate is up to 0.575mm/d. Results also showed that the homology of the rDNA ITS sequence is almost up to 99% between 0288 and the natural fruit body. Through this experiment, the best growth condition of 0288 is obtained and its fruit body is induced as pure cultural product. It would lay strong foundation for the artificial domestication of the wild T. matsutake.

scholarly journals Metal leaching from a spent catalyst by Alternaria alternata

2017 ◽  
Vol 2 (2) ◽  
pp. 221-231 ◽  
Author(s):  
Norma G. Rojas-Avelizapa ◽  
Jessica Otamendi-Valdez ◽  
Marlenne Gómez-Ramírez
Keyword(s):  
Alternaria Alternata ◽  
Culture Medium ◽  
Catalyst Concentration ◽  
Metal Removal ◽  
Culture Media ◽  
Hazardous Wastes ◽  
Spent Catalyst ◽  
Extracellular Medium ◽  
New Research ◽  
Selection Of

The study evaluated the leaching ability of the extracellular medium produced by a fungus, which was identified as Alternaria alternata. The study included the selection of a culture medium that favored the organic acid and siderophore production; sucrose and Czapek media were evaluated. Once the culture media was selected, bioleaching study of the catalyst at 1 and 5% continued, using only the extracellular media incubating at 30° C, 150 rpm during 7 days. Results indicated that the extracellular media produced by A. alternata in sucrose media decreases pH from 6 to 4.8; in this media siderophores were also produced. The study of metal bioleaching, using the extracellular media, showed that metals were leached at different extent, and it was observed that catalyst concentration had an important effect on the metal removal and on the order of the removal. It was found that at 1% catalyst and after 48 h of treatment, the following metals were removed, in descending order, V> Al> Si> Mo > Mg> Fe> Ni> As> Cr. This is the first report on the metal removal from the catalyst by the extracellular media of A. alternata which opens new research alternatives for the treatment of hazardous wastes with high metallic content.

scholarly journals Characterization of Arthrospira platensis cultured in wastewater of Clarias catfish farming media: DNA barcode, helical form, growth, and phycocyanin

2020 ◽  
Vol 21 (12) ◽  
Author(s):  
Marini Wijayanti ◽  
Mochamad Syaifudin ◽  
Yulisman Yulisman ◽  
Yully Nurianti ◽  
Anita Hidayani ◽  
...  
Keyword(s):  
Growth Rate ◽  
Phylogenetic Trees ◽  
Culture Medium ◽  
Culture Media ◽  
Dna Barcode ◽  
Arthrospira Platensis ◽  
Rrna Gene ◽  
Randomized Design ◽  
Density Growth

Abstract. Wijayanti M, Syafudin M, Yulisman, Nurianti Y, Hidayani A, Gofar N. 2020. Characterization of Arthrospira platensis cultured in wastewater of Clarias catfish farming media: DNA barcode, helical form, growth, and phycocyanin. Biodiversitas 21: 5872-5883. Arthrospira production technology in catfish waste media can be an alternative to reduce environmental pollution. However, some environmental factors such as nutrition, light, and water content can influence characterization of Arthrospira at the genetic and physiologic level. Arthrospira platensis is one of the phycocyanin-producing cyanobacteria and can be cultured using catfish culture wastewater. Water quality especially pH and salinity can effect of growth rate and residue of phycocyanin from Arthrospira platensis. This study aimed to identify the species and morphological forms of Arthrospira cultured using technical fertilizer and waste media, as well as to know the phylogenetic trees between species in this study and the GeneBank based on the 16S rRNA gene, and determine the optimum of pH and salinity required in the medium of catfish culture wastewater to phycocyanin maximum production of Arthrospira. The optimation of pH and salinity method used Completely Randomized Design (CRD) factorial with 2 factors consisting of the first factor with 3 treatments and the second factor with 4 treatments and 3 replications. The first factor was pH of culture medium i.e. pH 6.5 ± 0.2, pH 8.5 ± 0.2 and pH 10.5 ± 0.2. The second factor was salinity of culture medium, that was salinity 0 ppt (parts per thousand/‰), 10 ppt, 20 ppt, and 30 ppt. Parameters observed in Arthrospira include density, growth rate, rendement of phycocyanin, and decreased total nitrogen and phosphate content in culture media. The results showed that morphology Arthrospira cultured on technical fertilizer media (AF) had a longer and helix filament compared to Arthrospira cultured on waste media (AW) which showed several linear and shorter filaments. Both samples have a genetic distance of 0.068 (6.8%). Phylogenetic trees indicated that AF had a close relationship with Arthrospira platensis petH from Japan (bootstrap value 95%). While AW formed a separate sub-cluster of AF isolates and Arthrospira platensis petH from Japan (bootstrap value of 85%). The best treatment in this study was P2S3 (pH 8.5 ± 0.2 with salinity 20 ppt), which produced 0.867 grams maximum density, growth rate of 22.026 %.day-1 and 11.347 mg.g1 rendement of phycocyanin.

scholarly journals Cultivation of Autochthonous Microalgae for Biomass Feedstock: Growth Curves and Biomass Characterization for Their Use in Biorefinery Products

Energies ◽  
2021 ◽  
Vol 14 (15) ◽  
pp. 4567
Author(s):  
Juan Félix González ◽  
Teresa Belén Cuello ◽  
Antonio José Calderón ◽  
Manuel Calderón ◽  
Jerónimo González ◽  
...  
Keyword(s):  
Growth Rate ◽  
Culture Medium ◽  
Culture Media ◽  
Biogas Production ◽  
Growth Curves ◽  
Biomass Productivity ◽  
Climatic Conditions ◽  
Growth Effect ◽  
Batch Type ◽  
Fatty Acids Profile

In this work, the biomass productivity for biorefinery products and growth curves of three autochthonous microalgae collected in different reservoirs (“Scenedesmus sp.” (SSP), mixture of Scenedesmus sp., Chlorella minutissima, Chlorellas sp. and Nannochloropsis sp. named “La Orden” (LO) consortium and Chlorella minutissima named “Charca Brovales” (CB) consortium) were studied in a 5.5 L column laboratory photobioreactor. Two different culture media, Arnon culture (AM) and an agriculture fertilizer-based liquid medium (FM), have been used to evaluate the growth effect of the microalgae; it was found that the medium has a clear effect on the biomass productivity and growth rate, which ranged between 0.26–0.498 g L−1 d−1 and 0.288–0.864 d−1, respectively. In general, the elemental analysis and higher heating value of microalgae biomass for the three species were independent of the culture medium used for its growth, while their lipids and sugars content depended upon the species type and culture medium used in the cultivation. “La Orden” microalga was selected (given its best adaption to the climatic conditions) to study the biomass productivity and growth rate in two exterior photobioreactors (100 L column and 400 L flat panel), using FM as a medium, obtaining values of 0.116–0.266 g L−1 d−1 and 0.360–0.312 d−1, respectively. An automation and control system was designed to operate the exterior photobioreactors pilot plant. The lipid content of this microalga in these photobioreactors was lower than in the laboratory one, with a fatty acids profile with predominantly palmitic, oleic, linoleic and linolenic acids. Also, the fresh biomass collected from these photobioreactors was studied in a batch type digestion process for biogas production, obtaining a CH4 yield of 296 ± 23 L CH4 kgVSS−1 added with a reduction in percentage of COD and vs. of 50 ± 1% and 50 ± 1.7%, respectively.

scholarly journals Effects of Different Paclobutrazol-Cultar Concentrations on the Micropropagation of Sugarcane (Saccharum Officinarum) Variety CPCL99-4455

2018 ◽  
Vol 7 ◽  
pp. 1011-1018 ◽  
Author(s):  
Dion Daniels ◽  
Nidia Panti ◽  
David Guerra ◽  
Stephen Williams
Keyword(s):  
Growth And Development ◽  
Culture Medium ◽  
Culture Media ◽  
Saccharum Officinarum ◽  
Average Height ◽  
Ex Vitro ◽  
Agricultural Exports ◽  
Multiplication Coefficient ◽  
Important Cash Crop

Sugarcane (Saccharum officinarum), being an important cash crop in Belize, accounts for 60% of agricultural exports providing employment for thousands of Belizeans. This research was carried out to determine the effects of different Paclobutrazol (PBZ-Cultar) concentrations used in the culture media on in vitro multiplication of sugarcane variety CPCL99-4455. Three PBZ-Cultar concentrations were tested and compared with the control. The parameters evaluated to determine the effects of PBZ-Cultar were average height, number of dead leaves and multiplication coefficient. The plants from this experiment were planted in the acclimatization phase to determine if the use of PBZ-Cultar had any effect of the growth and development of the plants ex vitro. The results revealed that the culture medium supplemented with 0.08% PBZ-Cultar concentration had the best results both in the multiplication phase as well as the acclimatization phase.

scholarly journals Morphological Characterization of Corynespora cassiicola Isolates in Culture Media

2021 ◽  
Vol 13 (11) ◽  
pp. 74
Author(s):  
Jacqueline Dalbelo Puia ◽  
Adriano Thibes Hoshino ◽  
Estela Mariani Klein ◽  
Emily Danila De Almeida ◽  
Sandra Cristina Vigo ◽  
...  
Keyword(s):  
Growth Rate ◽  
Culture Medium ◽  
Culture Media ◽  
Agar Medium ◽  
Morphological Characterization ◽  
Corynespora Cassiicola ◽  
Cotton Production ◽  
Significance Level ◽  
Morphological Aspects ◽  
Host Origin

Corynespora cassiicola threatens soybean and cotton production in Brazil. The objective of this study was to evaluate cultural and morphological aspects of C. cassiicola isolated from soybean and cotton of different Brazilian regions, in culture media. The isolates were grown in PDA (Potato Dextrose Agar) and V8 juice agar media. The characteristics evaluated were: color, aspect, and growth rate of mycelia, as well as production and dimension of conidia, and number of septa per conidium. Culture media and isolates were compared using the Kruskal-Wallis or Tukey&rsquo;s test at 5% significance level. The mycelia of the isolates were predominantly dark gray and light brown. C. cassiicola isolates grew better in V8 juice agar medium, presenting a higher mycelial growth rate. In PDA medium, the production of conidia was higher in isolates from cotton, compared with soybean isolates. There was great variation in the production of conidia in V8 juice agar medium, regardless of the host origin. Conidia length and width varied for isolate and culture medium. The isolates of C. cassiicola coming from cotton presented a higher number of septa per conidium when grown in PDA medium. The morphological aspects of C. cassiicola vary depending on the host of origin and the culture medium.

scholarly journals OPTIMIZATION OF FACTORS AFFECTING THE Agrobacterium tumefaciens- MEDIATED TRANSFORMATION OF Eucalyptus saligna

2018 ◽  
Vol 41 (3) ◽  
Author(s):  
Yohana de Oliveira-Cauduro ◽  
Lais Gomes Adamuchio ◽  
João Carlos Bespalhok Filho ◽  
Isabel Rodrigues Gerhardt ◽  
Juliana Degenhardt-Goldbach ◽  
...  
Keyword(s):  
Gene Expression ◽  
Agrobacterium Tumefaciens ◽  
Culture Medium ◽  
Culture Media ◽  
Gus Gene ◽  
Eucalyptus Saligna ◽  
Factors Affecting ◽  
Gus Gene Expression ◽  
Positive Effect ◽  
Selection Of

ABSTRACT This study aimed to evaluate the effect of factors that may affect the genetic transformation of cotiledonary explants of Eucalyptus saligna mediated by EHA105 strain of Agrobacterium tumefaciens. The vector pBI121 carrying gus gene under control of 35S CaMV promoter was used. The effect of the following factors was evaluated: explant pre-culture, use of different antibiotics and presence of acetosyringone (AS) in co-culture media. An antioxidant solution was also used during excision, containing ascorbic acid (250mg.L-1), citric acid (25mg.L-1) and PVP-40 (1g.L-1). Pre-culture of the explants before the co-culture with bacteria was done over a 4-day period in MS culture medium supplemented with 4.4µM BAP and 2.7ìM NAA. After theco-culture period, three concentrations of kanamycin (12.5;25 and 50mg.L-1) combined with 300mg.L-1 Augmentin® in the culture medium were tested The influence of the antibiotic was also evaluated by keeping the explants in a medium containing 50mg.L-1 Km and 300mg.L-1 Augmentin® or 500mg.L-1 cefotaxime. It was concluded that Augmentin® stimulates organogenesis, that a Km concentration of 12.5mg.L-1 allows selection of explants transformed with gus gene and, finally, the addition of AS (50ìM) to the liquid and solid co-culture media has a positive effect on gus gene expression. Moreover, the use of an antioxidant solution during cotyledon excision is dispensable and the pre-culture of the explants has no effect on bud regeneration or gus gene expression. A transformation efficiency of 1.5% was reached.

P–275 Development of a prediction model using machine learning on small noncoding RNA biomarkers for non-invasive selection of high-quality embryos for the in vitro fertilization process

2021 ◽  
Vol 36 (Supplement_1) ◽  
Author(s):  
M Rabajdova ◽  
K Šoltys ◽  
M Kloc ◽  
O Slaby ◽  
S Toporcerova ◽  
...  
Keyword(s):  
Culture Medium ◽  
Embryo Quality ◽  
Massively Parallel Sequencing ◽  
Culture Media ◽  
Predictive Biomarkers ◽  
High Quality ◽  
Non Invasive ◽  
Embryo Culture Medium ◽  
Selection Of

Abstract Study question The aim of the study was to identify molecules in the embryo culture medium as important predictive biomarkers of high-quality embryos Summary answer The study identified 14 canonical iso-miRNA molecules that prognostically determine the quality of the embryo with a prediction accuracy with 95% sensitivity and 80% specificity. What is known already The quality of the embryo for the success of the IVF process is not specifically diagnosed, only morphological features (monitoring in the embryoscope) are considered. Embryo quality selection systems have likely reached their peak. The success rate of the IVF process is only 29%.; it is therefore necessary to look for other biomarkers. The oocyte itself can significantly predict the development of the early embryo,as it is a supplier of RNA and cellular mechanisms. However, collection follicular fluid is technically demanding. The probability of oocyte fertilization does not reach the required percentage therefore other embryological techniques multiply the economic costs. Study design, size, duration Women (n = 734) who visited the IVF centre were recruited for the study. Oocytes were collected from 54 of them and used for IVF. After 4/5-day embryo cultivation, the best quality embryo was selected and used for implantation into the uterus. The culture medium has been collected from 60 embryos during 3 years (2018–2020). Written informed consent was obtained from all patients. The study has been approved by the Ethical committee of the Košice governing region Participants/materials, setting, methods We used fresh/frozen culture media of embryos selected using an embryoscope. Further, information regarding the success of IVF, pregnancy and IVF failure was collected. Culture media libraries of noncoding small RNAs (miRNAs) were examined using massively parallel sequencing on the Illumina platform. Obtained data was processed with freely available bioinformatic tools and machine learning. For methods with different models, the number of predictive biomarkers and specific prognostic-predictive molecules were selected. Main results and the role of chance The main results of the study specifically identify ncRNA molecules that prognostically and predictively select a high-quality embryo suitable for IVF transmission from a low-quality embryo with 95% sensitivity and 80% specificity with an average accuracy of 85% in 4 different models. We also determined the minimum of 14 miRNA as prediction biomarkers. The developed model can predict embryo quality from the culture medium based on ncRNA results from sequence data and set the cut-off value for the expression and significance of individual miRNA molecules with respect to embryo quality. Furthermore, positive and negative correlations of miRNA molecules with different distributions in a high-quality embryo compared to a low-quality embryo were determined. The molecules identified in the embryo culture medium were organized according to their importance, resp. significance based on their significance coefficient. So far, there is no evidence of pending patents regarding the distribution of specific canonical miRNAs and iso-miRNA molecules analysed by massively parallel sequencing in terms of biological competence and embryo quality determination with multifactorial consideration of its variation. This is the first study focused on the success of the IVF process based on embryo quality prediction. Limitations, reasons for caution Exploratory data need to be validated in a larger scale study. Wider implications of the findings: The given miRNA molecules and the software model can be used as a safe,non-invasive diagnostic test for the selection of a highly competent embryo. Canonical and iso-miRNA molecules from the study can be used in other forms of diagnostic assays, such as specific embryo selection probes and, plate hybridization assay. Trial registration number non clinical trials

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