scholarly journals Manipulation of nitric oxide in an animal model of acute liver injury. The impact on liver and intestinal function

2007 ◽  
Vol 2 (2) ◽  
pp. 73-81
Author(s):  
Diya Adawi ◽  
F Behzad Kasravi ◽  
Göran Molin
Keyword(s):  
Nitric Oxide ◽  
Animal Model ◽  
Liver Injury ◽  
Acute Liver Injury ◽  
Intestinal Function ◽  
The Impact

Paracetamol induced acute liver injury in rabbits. Development of an experimental animal model

1998 ◽  
Vol 28 ◽  
pp. 224
Author(s):  
M. Vagena ◽  
D. Dimitroulopoulos ◽  
M. Demonakou ◽  
A. Papalois ◽  
G. Bersos ◽  
...  
Keyword(s):  
Animal Model ◽  
Liver Injury ◽  
Experimental Animal ◽  
Acute Liver Injury ◽  
Experimental Animal Model

scholarly journals Prevalence of acute liver injury and hypertransaminemia in patients with COVID-19: a protocol for a systematic review

BMJ Open ◽  
2020 ◽  
Vol 10 (7) ◽  
pp. e040517
Author(s):  
Gang Li ◽  
Yongxing Xu ◽  
Yi Tian Yang ◽  
Peng Fei Liu
Keyword(s):  
Systematic Review ◽  
Liver Injury ◽  
Acute Liver Injury ◽  
Epidemiological Data ◽  
Risk Of Bias ◽  
Published Data ◽  
Language Restriction ◽  
Ethical Approval ◽  
Meta Analyses ◽  
The Impact

IntroductionCOVID-19 has spread rapidly in China and around the world. Published studies have revealed that some patients with COVID-19 had abnormal liver function in laboratory tests. However, the results were inconsistent and the analysis of epidemiological data stratified by the severity of COVID-19 was not available in previous meta-analyses. Furthermore, these meta-analyses were suspected of overestimating the incidence of liver injury in patients with COVID-19 because some studies considered transaminase elevation as liver injury, which might partially result from cardiac and muscle injury. This systematic review aims to enrol published literatures related to COVID-19 without language restriction, analyse the data based on the severity of the COVID-19 and explore the impact of varied definitions of liver injury on the incidence of liver injury.Methods and analysisWe have conducted a preliminary search on PubMed and Excerpta Medica Database on 13 April 2020, for the studies published after December 2019 on the prevalence of acute liver injury and hypertransaminemia in patients with COVID-19. Two reviewers will independently screen studies, extract data and assess the risk of bias. We will estimate the pooled incidence of hypertransaminemia and acute liver injury in patients with COVID-19 by using the random-effects model. The I² test will be used to identify the extent of heterogeneity. Publication bias will be assessed by funnel plot and performing the Begg’s and Egger’s test if adequate studies are available. We will perform a risk of bias assessment using the Joanna Briggs Institute’s critical appraisal checklist.Ethics and disseminationSince this study will be based on the published data, it does not require ethical approval. The final results of this study will be published in a peer-reviewed journal.PROSPERO registration numberCRD42020179462.

Endoplasmic Reticulum Stress Increases Multidrug-resistance Protein 2 Expression and Mitigates Acute Liver Injury

2020 ◽  
Vol 20 (7) ◽  
pp. 548-557
Author(s):  
Wen-Ge Huang ◽  
Jun Wang ◽  
Yu-Juan Liu ◽  
Hong-Xia Wang ◽  
Si-Zhen Zhou ◽  
...  
Keyword(s):  
Endoplasmic Reticulum ◽  
Multidrug Resistance ◽  
Liver Injury ◽  
Protein Expression ◽  
Er Stress ◽  
Acute Liver Injury ◽  
Multidrug Resistance Protein ◽  
Functional Involvement ◽  
Resistance Protein ◽  
The Impact

Background: Multidrug-resistance protein (MRP) 2 is a key membrane transporter that is expressed on hepatocytes and regulated by nuclear factor kappa B (NF-κB). Interestingly, endoplasmic reticulum (ER) stress is closely associated with liver injury and the activation of NF-κB signaling. Objective: Here, we investigated the impact of ER stress on MRP2 expression and the functional involvement of MRP2 in acute liver injury. Methods: ER stress, MRP2 expression, and hepatocyte injury were analyzed in a carbon tetrachloride (CCl4)-induced mouse model of acute liver injury and in a thapsigargin (TG)-induced model of ER stress. Results: CCl4 and TG induced significant ER stress, MRP2 protein expression and NF- κB activation in mice and LO2 cells (P < 0.05). Pretreatment with ER stress inhibitor 4- phenyl butyric acid (PBA) significantly mitigated CCl4 and TG-induced ER stress and MRP2 protein expression (P < 0.05). Moreover, pretreatment with pyrrolidine dithiocarbamic acid (PDTC; NF-κB inhibitor) significantly inhibited CCl4-induced NF-κB activation and reduced MRP2 protein expression (1±0.097 vs. 0.623±0.054; P < 0.05). Furthermore, hepatic downregulation of MRP2 expression significantly increased CCl4- induced ER stress, apoptosis, and liver injury. Conclusion: ER stress enhances intrahepatic MRP2 protein expression by activating NF-κB. This increase in MRP2 expression mitigates ER stress and acute liver injury.

scholarly journals Triazinone herbicide metribuzin induced acute liver injury: A study of animal model

2018 ◽  
Vol 7 (4) ◽  
pp. 152
Author(s):  
Derouiche Samir ◽  
Rezzag mohcenOm Selma ◽  
Serouti Asma
Keyword(s):  
Animal Model ◽  
Liver Injury ◽  
Acute Liver Injury

scholarly journals Hepatoprotective Effects of Different Extracts From Triphala Against CCl4-Induced Acute Liver Injury in Mice

2021 ◽  
Vol 12 ◽  
Author(s):  
Xichuan Wei ◽  
Chuanhong Luo ◽  
Yanan He ◽  
Haozhou Huang ◽  
Fei Ran ◽  
...  
Keyword(s):  
Liver Injury ◽  
Mouse Model ◽  
Protein Expression ◽  
Acute Liver Injury ◽  
Quinone Oxidoreductase ◽  
Tnf Α ◽  
Gene And Protein Expression ◽  
Wide Range ◽  
Hepatoprotective Effects ◽  
The Impact

Background:Triphala is a traditional polyherbal formula used in Indian Ayurvedic and Chinese Tibetan medicine. A wide range of biological activities have been attributed to Triphala, but the impact of various extraction methods on efficacy has not been determined.Purpose: The study aimed to evaluate Triphala extracts obtained by various methods for their hepatoprotective effects and molecular mechanisms in a mouse model of carbon tetrachloride (CCl4)-induced liver injury.Methods: HPLC fingerprinting was used to characterize the chemical characteristics of Triphala extracts obtained by (a) 0.5 h ultrasonication, (b) 2 h reflux, and (c) 4 h reflux. Hepatoprotective efficacy was evaluated in a mouse model of CCl4-induced liver damage. Serum levels of alanine transaminase (ALT) and aspartate aminotransferase (AST) were measured, as well as the liver antioxidant and inflammatory markers malondialdehyde superoxide dismutase glutathione peroxidase (GSH-Px), TNF-α, and IL-6. Gene and protein expression of Nrf-2 signaling components Nrf-2, heme oxygenase (HO-1), and NADPH Quinone oxidoreductase (NQO-1) in liver tissue were evaluated by real-time PCR and western blotting.Results: Chemical analysis showed a clear difference in content between extracts produced by ultrasonic and reflux methods. The pharmacological analysis showed that all three Triphala extracts reduced ALT, AST, MDA, TNF-α, and IL-6 levels and increased SOD and GSH-Px. Triphala extracts also induced transcript and protein expression of Nrf-2, HO-1, and NQO-1.Conclusion: Triphala extract prevents CCl4-induced acute liver injury. The ultrasonic extract of Triphala was most effective, suggesting that hepatoprotection may be related to the larger tannins via activation of Nrf-2 signaling.

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