Faculty Opinions recommendation of Chemoattractants and chemorepellents act by inducing opposite polarity in phospholipase C and PI3-kinase signaling.

During embryonic development, cell movement is orchestrated by a multitude of attractants and repellents. Chemoattractants applied as a gradient, such as cAMP with Dictyostelium discoideum or fMLP with neutrophils, induce the activation of phospholipase C (PLC) and phosphoinositide 3 (PI3)-kinase at the front of the cell, leading to the localized depletion of phosphatidylinositol 4,5-bisphosphate (PI[4,5]P2) and the accumulation of phosphatidylinositol-3,4,5-trisphosphate (PI[3,4,5]P3). Using D. discoideum, we show that chemorepellent cAMP analogues induce localized inhibition of PLC, thereby reversing the polarity of PI(4,5)P2. This leads to the accumulation of PI(3,4,5)P3 at the rear of the cell, and chemotaxis occurs away from the source. We conclude that a PLC polarity switch controls the response to attractants and repellents.

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Translocation of beta-arrestin2 by the PTH type 2 receptor is independent from its ligand-induced cAMP signaling, phospholipase C-dependent signaling, or MAP kinase signaling

Experimental and Clinical Endocrinology & Diabetes ◽

10.1055/s-2005-862991 ◽

2005 ◽

Vol 113 (S 1) ◽

Author(s):

E Blind ◽

S Meyer ◽

S Hahner ◽

B Allolio ◽

U Heindel

Keyword(s):

Map Kinase ◽

Phospholipase C ◽

Camp Signaling ◽

Kinase Signaling ◽

Map Kinase Signaling

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Drosophila Spidey/Kar Regulates Oenocyte Growth via PI3-Kinase Signaling

PLoS Genetics ◽

10.1371/journal.pgen.1006154 ◽

2016 ◽

Vol 12 (8) ◽

pp. e1006154 ◽

Cited By ~ 14

Author(s):

Einat Cinnamon ◽

Rami Makki ◽

Annick Sawala ◽

Leah P. Wickenberg ◽

Gary J. Blomquist ◽

...

Keyword(s):

Pi3 Kinase ◽

Kinase Signaling

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PI3 kinase signaling pathway in hematopoietic cancers: A glance in miRNA's role

Journal of Clinical Laboratory Analysis ◽

10.1002/jcla.23725 ◽

2021 ◽

Vol 35 (4) ◽

Author(s):

Elham Roshandel ◽

Leila Noorazar ◽

Behrouz Farhadihosseinabadi ◽

Mahshid Mehdizadeh ◽

Mohammad Hossein Kazemi ◽

...

Keyword(s):

Signaling Pathway ◽

Pi3 Kinase ◽

Kinase Signaling ◽

Kinase Signaling Pathway ◽

Hematopoietic Cancers

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178 PI3-kinase signaling modulates the induction of MHC molecules by IFN-gamma

Journal of Investigative Dermatology ◽

10.1016/j.jid.2018.03.183 ◽

2018 ◽

Vol 138 (5) ◽

pp. S30

Author(s):

S. Chandrasekaran ◽

M. Sasaki ◽

B. Sapkota ◽

B. Pollack

Keyword(s):

Pi3 Kinase ◽

Kinase Signaling ◽

Ifn Gamma ◽

Mhc Molecules

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Wiskott-Aldrich Syndrome Interacting Protein Deficiency Uncovers the Role of the Co-receptor CD19 as a Generic Hub for PI3 Kinase Signaling in B Cells

Immunity ◽

10.1016/j.immuni.2015.09.004 ◽

2015 ◽

Vol 43 (4) ◽

pp. 660-673 ◽

Cited By ~ 40

Author(s):

Selina Jessica Keppler ◽

Francesca Gasparrini ◽

Marianne Burbage ◽

Shweta Aggarwal ◽

Bruno Frederico ◽

...

Keyword(s):

B Cells ◽

Pi3 Kinase ◽

Protein Deficiency ◽

Kinase Signaling ◽

Interacting Protein ◽

Wiskott Aldrich Syndrome

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IGFs stimulate zebrafish cell proliferation by activating MAP kinase and PI3-kinase-signaling pathways

AJP Regulatory Integrative and Comparative Physiology ◽

10.1152/ajpregu.2001.280.4.r1230 ◽

2001 ◽

Vol 280 (4) ◽

pp. R1230-R1239 ◽

Cited By ~ 69

Author(s):

Kasiani C. Pozios ◽

Jun Ding ◽

Brian Degger ◽

Zee Upton ◽

Cunming Duan

Keyword(s):

Cell Proliferation ◽

Protein Kinase ◽

Dna Synthesis ◽

Signaling Pathways ◽

Kinase Inhibitor ◽

Pi3 Kinase ◽

Dependent Manner ◽

Embryonic Cells ◽

Kinase Signaling ◽

Igf I

Insulin-like growth factor (IGF)-I and -II have been cloned from a number of teleost species, but their cellular actions in fish are poorly defined. In this study, we show that both IGF-I and -II stimulated zebrafish embryonic cell proliferation and DNA synthesis in a concentration-dependent manner, whereas insulin had little mitogenic activity. Affinity cross-linking and immunoblotting studies revealed the presence of IGF receptors with the characteristics of the mammalian type I IGF receptor. Competitive binding assay results indicated that the binding affinities of the zebrafish IGF-I receptors to IGF-I, IGF-II, and insulin are 1.9, 2.6, and >190 nM, indicating that IGF-I and -II bind to the IGF-I receptor(s) with approximately equal high affinity. To further investigate the cellular mechanism of IGF actions, we have studied the effects of IGFs on two major signal transduction pathways: mitogen-activated protein kinase (MAPK) and phosphatidylinositol 3-kinase (PI3 kinase). IGFs activated MAPK in zebrafish embryonic cells in a dose-dependent manner. This activation occurred within 5 min of IGF-I stimulation and disappeared after 1 h. IGF-I also caused a concentration-dependent activation of protein kinase B, a downstream target of PI3 kinase, this activation being sustained for several hours. Inhibition of MAPK activation by the MAPK kinase inhibitor PD-98059 inhibited the IGF-I-stimulated DNA synthesis. Similarly, use of the PI3 kinase inhibitor LY-294002 also inhibited IGF-I-stimulated DNA synthesis. When both the MAPK and PI3 kinase pathways were inhibited using a combination of these compounds, the IGF-I-stimulated DNA synthesis was completely negated. These results indicate that both IGF-I and -II are potent mitogens for zebrafish embryonic cells and that activation of both the MAPK and PI3 kinase-signaling pathways is required for the mitogenic action of IGFs in zebrafish embryonic cells.

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