Faculty Opinions recommendation of Limited nesting stress alters maternal behavior and in vivo intestinal permeability in male wistar pup rats.

AbstractThe intestinal barrier plays a crucial role in maintaining gut health, and an increased permeability has been linked to several intestinal and extra-intestinal disorders. There is an increasing demand for interventions aimed at strengthening this barrier and for in vivo challenge models to assess their efficiency. This study investigated the effect of sauna-induced dehydration on intestinal barrier function (clinicaltrials.gov: NCT03620825). Twenty healthy subjects underwent three conditions in random order: (1) Sauna dehydration (loss of 3% body weight), (2) non-steroidal anti-inflammatory drug (NSAID) intake, (3) negative control. Intestinal permeability was assessed by a multi-sugar urinary recovery test, while intestinal damage, bacterial translocation and cytokines were assessed by plasma markers. The sauna dehydration protocol resulted in an increase in gastroduodenal and small intestinal permeability. Presumably, this increase occurred without substantial damage to the enterocytes as plasma intestinal fatty acid-binding protein (I-FABP) and liver fatty acid-binding protein (L-FABP) were not affected. In addition, we observed significant increases in levels of lipopolysaccharide-binding protein (LBP), IL-6 and IL-8, while sCD14, IL-10, IFN-ɣ and TNF-α were not affected. These results suggest that sauna dehydration increased intestinal permeability and could be applied as a new physiological in vivo challenge model for intestinal barrier function.

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Intestinal permeability in allergic rats: nerve involvement in antigen-induced changes

AJP Gastrointestinal and Liver Physiology ◽

10.1152/ajpgi.1993.264.4.g617 ◽

1993 ◽

Vol 264 (4) ◽

pp. G617-G623 ◽

Cited By ~ 7

Author(s):

S. E. Crowe ◽

K. Soda ◽

A. M. Stanisz ◽

M. H. Perdue

Keyword(s):

Intestinal Permeability ◽

Fold Increase ◽

Serum Levels ◽

Baseline Period ◽

Antigen Challenge ◽

51Cr Edta ◽

Neural Factors ◽

Induced Changes ◽

In Vivo Uptake

In vivo uptake of the probe 51Cr-labeled EDTA from the jejunum of egg albumin (EA)-sensitized rats was compared with controls at baseline and after intraluminal antigen challenge. Probe recovery in blood was 60-80% greater in sensitized animals during the baseline period, suggesting that sensitization resulted in increased intestinal permeability. Sensitized, but not control, rats demonstrated a 15-fold increase in 51Cr-EDTA uptake after intraluminal antigen; no change occurred with an unrelated protein. Macromolecular recovery was also enhanced in sensitized animals, since serum levels of immunoreactive EA were elevated 14-fold compared with controls. Antigen challenge was accompanied by biochemical (protease release) and morphological (reduced numbers) evidence of mast cell degranulation in sensitized rats. The neurotoxin tetrodotoxin (applied directly to ligated jejunal segments) inhibited EA-induced uptake of 51Cr-EDTA and antigen. In isolated jejunum from sensitized rats, tetrodotoxin reduced secretory responses to luminal, but not serosal, antigen. These results indicate that neural factors may influence the uptake of molecules from the gut lumen during intestinal anaphylaxis.

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Intestinal Permeability Assays: a Review

Russian Journal of Gastroenterology Hepatology Coloproctology ◽

10.22416/1382-4376-2021-31-1-20-30 ◽

2021 ◽

Vol 31 (1) ◽

pp. 20-30

Author(s):

A. A. Iakupova ◽

S. R. Abdulkhakov ◽

R. K. Zalyalov ◽

A. G. Safin ◽

R. A. Abdulkhakov

Keyword(s):

Intestinal Permeability ◽

Ex Vivo ◽

Venous Blood ◽

Intestinal Barrier ◽

Intestinal Lumen ◽

Alcoholic Fatty Liver ◽

Key Points ◽

Assessment Techniques ◽

Portal Venous Blood

Aim. A literature review of intestinal permeability assessment techniques.Key points. The intestinal barrier is a functional entity separating the intestinal lumen and internal body, and intestinal permeability is a measure of the barrier functionality. The intestinal barrier integrity and permeability assays differ by the application setting (in vivo or ex vivo), subject (human or animal), marker molecules used to assess permeability (ions, various size carbohydrates, macromolecules, antigens, bacterial products and bacteria), biomaterial for the marker concentration assays (peripheral blood, portal venous blood, urine, stool). Despite a great variety of methods for assessing intestinal permeability, their clinical application requires further studies due to a lack of standardisation, the complexity of selected techniques and occasional limited reliability of results.Conclusion. Further investigation and improvement of intestinal permeability assays is required. The assay and result standardisation will facilitate practice in functional and organic intestinal diseases, as well as allergies, diabetes mellitus, non-alcoholic fatty liver disease and some other illnesses.

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Fecal Enterobacteriales enrichment is associated with increased in vivo intestinal permeability in humans

Physiological Reports ◽

10.14814/phy2.13649 ◽

2018 ◽

Vol 6 (7) ◽

pp. e13649 ◽

Cited By ~ 11

Author(s):

Camilla Pedersen ◽

Umer Z. Ijaz ◽

Edith Gallagher ◽

Felicity Horton ◽

Richard J. Ellis ◽

...

Keyword(s):

Intestinal Permeability

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Uses and Abuses of Intestinal Permeability Measurements

Canadian Journal of Gastroenterology ◽

10.1155/1988/867416 ◽

1988 ◽

Vol 2 (3) ◽

pp. 127-132 ◽

Cited By ~ 20

Author(s):

Timothy J. Peters ◽

Ingvar Bjarnason

Keyword(s):

Ethylene Glycol ◽

Intestinal Permeability ◽

Major Complication ◽

Chronic Administration ◽

Intestinal Damage ◽

Poly Ethylene Glycol ◽

Sugar Mixtures ◽

Poly Ethylene

Intestinal permeability has been assessed with three different classes of permeability probes, viz various sugar mixtures,51Cr-EDTA and poly(ethylene glycol). The former two methods are having increasing clinical applications in the screening and assessment of small intestinal damage and51Cr-EDTA is now the preferred probe for routine clinical use. Poly(ethylene glycol)s have numerous disadvantages and are not recommended. Probes may be used both in vitro and in vivo and have been applied to a wide variety of clinical problems. In particular, NSAID induced enteropathy, a major complication of the chronic administration of these widely-used drugs, was recognized for the first time with51Cr-EDTA permeability measurements. The cytoprotective role of various prostanoids was also clearly demonstrated using51Cr-EDTA. It is anticipated that measurement of intestinal permeability will play an increasing role in clinical and research investigation and in the monitoring of intestinal disease.

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S1781 Mechanisms of TNF-α-Induced Increase in Intestinal Permeability in an In-Vivo Mouse Model: Role of Myosine Light Chain Kinase

Gastroenterology ◽

10.1016/s0016-5085(10)61252-8 ◽

2010 ◽

Vol 138 (5) ◽

pp. S-273

Author(s):

Shuhong Guo ◽

Matthew A. Smith ◽

Karol Dokladny ◽

Dongmei Ye ◽

Rana Al-Sadi ◽

...

Keyword(s):

Mouse Model ◽

Intestinal Permeability ◽

Light Chain ◽

Tnf Α

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Microbiota-derived tryptophan indoles increase after gastric bypass surgery and reduce intestinal permeability in vitro and in vivo

Neurogastroenterology & Motility ◽

10.1111/nmo.13178 ◽

2017 ◽

Vol 30 (2) ◽

pp. e13178 ◽

Cited By ~ 26

Author(s):

M. Jennis ◽

C. R. Cavanaugh ◽

G. C. Leo ◽

J. R. Mabus ◽

J. Lenhard ◽

...

Keyword(s):

Gastric Bypass ◽

Intestinal Permeability ◽

Bypass Surgery ◽

Gastric Bypass Surgery

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Effect of sodium ion coupled nutrient transport on intestinal permeability in chronically catheterised rats

Gut ◽

10.1136/gut.43.1.22 ◽

1998 ◽

Vol 43 (1) ◽

pp. 22-28 ◽

Cited By ~ 3

Author(s):

M R Uhing

Keyword(s):

Intestinal Permeability ◽

Nutrient Transport ◽

Intestinal Lumen ◽

Sprague Dawley ◽

In Vitro Methods ◽

Physiological Conditions ◽

Sprague Dawley Rats ◽

Rate Of Absorption

Background—The significance of Na-nutrient cotransport induced alterations in paracellular permeability is controversial. Most previous studies have measured intestinal permeability using in vitro methods or in vivo methods immediately after surgical bowel manipulation, and therefore may not be applicable to normal physiological conditions.Aims—To determine whether activation of Na coupled nutrient transport increases intestinal permeability under normal physiological conditions.Methods—The effect of Na-nutrient cotransport on intestinal permeability was measured in unrestrained, unanaesthetised, chronically catheterised male Sprague-Dawley rats using two different methods: measurement of the rate of absorption of passively absorbed hexoses, mannitol and l-glucose; and measurement of the mannitol:urea diffusion ratio.Results—l-Glucose and mannitol absorption were not increased in the presence ofd-glucose, alanine, maltose, or peptides. The mannitol:urea diffusion ratio was not increased by the presence ofd-glucose. The presence of d-glucose in the intestinal lumen for 20 minutes did not alter intestinal permeability.Conclusions—Under normal physiological conditions, Na coupled nutrient transport does not increase intestinal permeability.

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