Pigmento vermelho produzido por Serratia Marcescens UFPEDA 223: otimização e avaliação da atividade antimicrobiana / Red pigment produced by Serratia Marcescens UFPEDA 223: optimization and evaluation of the antimicrobial activity

2021 ◽  
Vol 7 (8) ◽  
pp. 85342-85357
Author(s):  
Luana Farias de Aguiar ◽  
Amanda Maria da Silva ◽  
Michelle Gomes da Silva ◽  
Richardson Silveira Trindade da Silva ◽  
Diego Santa Clara Marques ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Serratia Marcescens ◽  
Red Pigment

scholarly journals THIAMINE – INDUCED FORMATION OF THE MONOPYRROLE MOIETY OF PRODIGIOSIN

2007 ◽  
Vol 4 (4) ◽  
pp. 622-627 ◽  
Author(s):  
Baghdad Science Journal
Keyword(s):  
Serratia Marcescens ◽  
Yeast Extract ◽  
Type Strain ◽  
The Other ◽  
Red Pigment ◽  
Enhanced Production ◽  
Pyrimidine Moiety ◽  
Glycerol Medium

Thiamine stimulates the production of a red pigment , which is chromatographically and spectrophotometrically identical to prodigiosin , by growing cultures of serratia marcescens mutant 9-3-3 . this mutant is blocked in the formation of 2- methyl -3- amyl pyrorol( MAP),the monopyrrole moiety of prodigiosin , but accumulates 4-methoxy-2, 2-bipyrrole -5- carboxaldehyde (MBC) and can couple this compound with( MAP) to form prodigiosin . Addition of thiamine caused production of( MAP) , and as little as 0.02 mg of thiamine / ml in peptone- glycerol medium stimulated production of measurable amounts of prodigiosin. Phosphate saltes and another type of peptone decreased the thiamine- induced formation of prodigiosin ,yeast extract and glycerol enhanced formation of this substance. Thiamine also enhanced production of prodigiosin by wiled – type Strain Nima of S. marcescens . The pyrimidine moiety of thiamine was also 10% as effective as the vitamin ; the thiazol moiety only 4% , and the two moieties together , 25% . Thiamine did not stimulate production of prodigiosin biosynthesis as strain 9-3-3 . This is not surprising since strain 9-3-3 originated as a result of two mutational events one event may involve thiamine directly , and the other may involve the biosynthesis of( MAP).

Topics in Clinical Microbiology

1983 ◽  
Vol 4 (6) ◽  
pp. 469-471 ◽  
Author(s):  
Charles W. Stratton ◽  
Rosemary Verrall
Keyword(s):  
Serratia Marcescens ◽  
Nosocomial Infections ◽  
Clinical Microbiology ◽  
Biological Marker ◽  
Biological Warfare ◽  
Red Pigment ◽  
Us Army ◽  
Starchy Food ◽  
Serious Infections ◽  
The Us

Serratia marcescens has an interesting and many-faceted history. The presence of the red pigmented organism on starchy food products long had been noted and was thought to be blood; its presence on consecrated wafers was thought to be the blood of Christ. It was not until 1819 that Bizio, a pharmacist, demonstrated that a microorganism was responsible for the red pigment. He named this microorganism Serratia marcescens; Serratia in honor of Serafino Serrati, an Italian physicist who invented the steamboat and marcescens from the Latin, “to decay.”Because of the pigment production and because Serratia marcescens was believed to be a benign, saprophytic organism, it was used widely as a biological marker from 1906 to 1968. This use has been controversial in regard to experiments conducted by the US Army involving biological warfare techniques. In addition, the recognition in the 1950s of the ability of this organism to cause nosocomial infections has curtailed its use as a biological marker. Serratia marcescens is now recognized as a cause of serious infections in man. It has been implicated in practically every kind of infection and is a particularly serious problem with nosocomial infections.

scholarly journals Inactivation of the Major Hemolysin Gene Influences Expression of the Nonribosomal Peptide Synthetase Gene swrA in the Insect Pathogen Serratia sp. Strain SCBI

2017 ◽  
Vol 199 (21) ◽  
Author(s):  
Lauren M. Petersen ◽  
Kaitlyn LaCourse ◽  
Tim A. Schöner ◽  
Helge Bode ◽  
Louis S. Tisa
Keyword(s):  
Antimicrobial Activity ◽  
Serratia Marcescens ◽  
Virulence Factors ◽  
Nonribosomal Peptide Synthetase ◽  
Mrna Levels ◽  
Insect Pathogen ◽  
Nonribosomal Peptide ◽  
Content Type ◽  
Hemolysin Gene ◽  
Peptide Synthetase

ABSTRACT Hemolysins are important virulence factors for many bacterial pathogens, including Serratia marcescens. The role of the major hemolysin gene in the insect pathogen Serratia sp. strain SCBI was investigated using both forward and reverse-genetics approaches. Introduction of the major hemolysin gene into Escherichia coli resulted in a gain of both virulence and hemolytic activity. Inactivation of this hemolysin in Serratia sp. SCBI resulted in a loss of hemolysis but did not attenuate insecticidal activity. Unexpectedly, inactivation of the hemolysin gene in Serratia sp. SCBI resulted in significantly increased motility and increased antimicrobial activity. Reverse transcription-quantitative PCR (qRT-PCR) analysis of mutants with a disrupted hemolysin gene showed a dramatic increase in mRNA levels of a nonribosomal peptide synthetase gene, swrA, which produces the surfactant serrawettin W2. Mutation of the swrA gene in Serratia sp. SCBI resulted in highly varied antibiotic activity, motility, virulence, and hemolysis phenotypes that were dependent on the site of disruption within this 17.75-kb gene. When introduced into E. coli, swrA increases rates of motility and confers antimicrobial activity. While it is unclear how inactivation of the major hemolysin gene influences the expression of swrA, these results suggest that swrA plays an important role in motility and antimicrobial activity in Serratia sp. SCBI. IMPORTANCE The opportunistic Gram-negative bacteria of the genus Serratia are widespread in the environment and can cause human illness. A comparative genomics analysis between Serratia marcescens and a new Serratia species from South Africa, termed Serratia sp. strain SCBI, shows that these two organisms are closely related but differ in pathogenesis. S. marcescens kills Caenorhabditis nematodes, while Serratia sp. SCBI is not harmful and forms a beneficial association with them. This distinction presented the opportunity to investigate potential differences in regulation of common virulence mechanisms between these two species. With the emergence of antibiotic-resistant microorganisms, there is a widespread need to understand the regulation of pathogenesis. The significance of this study is the presentation of evidence for cross-pathway regulation of virulence factors and how the elimination of one mechanism may be compensated for by the upregulation of others.

scholarly journals Red Pigment Producing Serratia marcescens Isolated from Abalone (Haliotis discus)

KSBB Journal ◽  
2016 ◽  
Vol 31 (4) ◽  
pp. 214-218 ◽  
Author(s):  
YuJin Shin ◽  
Chang-Ho Kang ◽  
Jae-Seong So
Keyword(s):  
Serratia Marcescens ◽  
Red Pigment ◽  
Haliotis Discus ◽  
Abalone Haliotis Discus

The red pigment prodigiosin is not an essential virulence factor in entomopathogenic Serratia marcescens

2016 ◽  
Vol 136 ◽  
pp. 92-94 ◽  
Author(s):  
Wei Zhou ◽  
JingHua Li ◽  
Jie Chen ◽  
XiaoYuan Liu ◽  
TingTing Xiang ◽  
...  
Keyword(s):  
Serratia Marcescens ◽  
Virulence Factor ◽  
Red Pigment

Antimicrobial activity of prodigiosin isolated from Serratia marcescens UFPEDA 398

2014 ◽  
Vol 31 (2) ◽  
pp. 399-406 ◽  
Author(s):  
J. C. Lapenda ◽  
P. A. Silva ◽  
M. C. Vicalvi ◽  
K. X. F. R. Sena ◽  
S. C. Nascimento
Keyword(s):  
Antimicrobial Activity ◽  
Serratia Marcescens

scholarly journals Biological Potential and Mechanism of Prodigiosin from Serratia marcescens Subsp. lawsoniana in Human Choriocarcinoma and Prostate Cancer Cell Lines

2018 ◽  
Vol 19 (11) ◽  
pp. 3465 ◽  
Author(s):  
Dan Li ◽  
Jun Liu ◽  
Xin Wang ◽  
Di Kong ◽  
Wei Du ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Serratia Marcescens ◽  
Cell Lines ◽  
Prostate Cancer Cell ◽  
Red Pigment ◽  
Anticancer Activities ◽  
Prostate Cancer Cell Lines ◽  
Pc3 Cells

Tripyrrole molecules have received renewed attention due to reports of numerous biological activities, including antifungal, antibacterial, antiprotozoal, antimalarial, immunosuppressive, and anticancer activities. In a screen of bacterial strains with known toxicities to termites, a red pigment-producing strain, HDZK-BYSB107, was isolated from Chamaecyparis lawsoniana, which grows in Oregon, USA. Strain HDZK-BYSB107 was identified as Serratia marcescens subsp. lawsoniana. The red pigment was identified as prodigiosin using ultraviolet absorption, LC-MS, and 1H-NMR spectroscopy. The bacterial prodigiosin had an inhibitory effect on both Gram-negative and Gram-positive bacteria. The main objective of this study was to explore the anticancer activities and mechanism of strain HDZK-BYSB107 prodigiosin by using human choriocarcinoma (JEG3) and prostate cancer cell lines (PC3) in vitro and JEG3 and PC3 tumor-bearing nude mice in vivo. In vitro anticancer activities showed that the bacterial prodigiosin induced apoptosis in JEG3 cells. In vivo anticancer activities indicated that the prodigiosin significantly inhibited the growth of JEG3 and PC3 cells, and the inhibitory activity was dose and time dependent. The anticancer efficacy of the bacterial prodigiosin on JEG3 and PC3 cells, JEG3 and PC3 tumor exhibited a correlation with the down regulation of the inhibitor of IAP family, including XIAP, cIAP-1 and cIAP-2, and the activation of caspase-9 and caspase-3 accompanied by proteolytic degradation of poly (ADP-ribose)-polymerase. The expressions of P53 and Bax/Bcl-2 in JEG3 and PC3 cells were significantly higher than in untreated groups. Our results indicated that the bacterial prodigiosin extracted from C. lawsoniana is a promising molecule due to its potential for therapeutic applications.

scholarly journals Prodigiosin of Serratia marcescens ZPG19 Alters the Gut Microbiota Composition of Kunming Mice

Molecules ◽  
2021 ◽  
Vol 26 (8) ◽  
pp. 2156
Author(s):  
Xue Li ◽  
Xinfeng Tan ◽  
Qingshuang Chen ◽  
Xiaoling Zhu ◽  
Jing Zhang ◽  
...  
Keyword(s):  
Serratia Marcescens ◽  
Intestinal Microbiota ◽  
High Performance ◽  
High Throughput Sequencing ◽  
Lactobacillus Reuteri ◽  
Intestinal Inflammation ◽  
Charge Ratio ◽  
Sequencing Analysis ◽  
Red Pigment ◽  
Abundance And Diversity

Prodigiosin is a red pigment produced by Serratia marcescens with anticancer, antimalarial, and antibacterial effects. In this study, we extracted and identified a red pigment from a culture of S. marcescens strain ZPG19 and investigated its effect on the growth performance and intestinal microbiota of Kunming mice. High-performance liquid chromatography/mass spectrometry revealed that the pigment had a mass-to-charge ratio (m/z) of 324.2160, and thus it was identified as prodigiosin. To investigate the effect of prodigiosin on the intestinal microbiota, mice (n = 5) were administered 150 μg/kg/d prodigiosin (crude extract, 95% purity) via the drinking water for 18 days. Administration of prodigiosin did not cause toxicity in mice. High-throughput sequencing analysis revealed that prodigiosin altered the cecum microbiota abundance and diversity; the relative abundance of Desulfovibrio significantly decreased, whereas Lactobacillus reuteri significantly increased. This finding indicates that oral administration of prodigiosin has a beneficial effect on the intestinal microbiota of mice. As prodigiosin is non-toxic to mouse internal organs and improves the mouse intestinal microbiota, we suggest that it is a promising candidate drug to treat intestinal inflammation.

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