scholarly journals Evaluación del índice de frecuencia, sensibilidad y especificidad entre iELISA y PAL en el diagnóstico de brucelosis bovina en tanque de leche / Evaluation of the frequency, sensitivity and specificity index between iELISA and PAL in the diagnosis of bovine brucellosis in a milk tank

2021 ◽  
Vol 4 (4) ◽  
pp. 6246-6253
Author(s):  
Jesús Borrego Jiménez ◽  
Aurora Martínez Romero ◽  
José Luis Ortega Sánchez ◽  
Jesús Eduardo Luna Martínez ◽  
José de Jesús Alba-Romero
Keyword(s):  
Sensitivity And Specificity ◽  
Brucella Abortus ◽  
Bovine Brucellosis ◽  
Frequency Sensitivity ◽  
Holstein Friesian ◽  
Para Determinar

El objetivo del presente proyecto fue diagnosticar brucelosis bovina en tanque de leche con la Prueba Anillo de Leche (PAL) y ELISA indirecto (iELISA), así el índice de frecuencias para determinar una comparativa entre ambas pruebas para establecer costo beneficio, tiempo de análisis, precisión en los resultados. Se seleccionó una población de 276 bovinos hembra adultos lecheros raza Holstein-Friesian de 38 a 48 meses de edad. La brucelosis se diagnosticó en muestras de leche con la prueba PAL y iELISA, bajo los protocolos establecidos. Los resultados obtenidos con la técnica PAL se tuvieron 77 muestras negativas, 15 positivas y 4 control de la placa 1 con un total de 96 muestras; se obtuvieron 86 negativos, 6 positivos y 4 control de la placa 2 con un total de 96 muestras; y 83 negativos, 9 positivos y 4 control de la placa 3 con un total de 96 muestras. Se tuvo mayor porcentaje de detección 82.8% a Brucella abortus en las muestras analizadas por medio de iELISA que con las muestras analizadas con PAL 46.92%. Se concluye que el índice de frecuencias para determinar una comparativa entre ambas pruebas para determinar costo beneficio, entre PAL y iELISA, tiempo de análisis la PAL es más específica 48.93% que la prueba iELISA 19.83% y adicionalmente la prueba iELISA es más sensible 76.92% que PAL 14.85% para diagnosticar brucelosis bovina en tanque de leche. Se deduce que la prueba iELISA presento más frecuencias positivas, por lo que es una prueba más sensible que la PAL. Sin embargo, PAL es más rápida de realizar y es menos costosa que iELISA.

scholarly journals Bovine brucellosis in slaughterhouses controlled by Federal and Municipal Inspection Services in the state of Maranhão, Brazil

2019 ◽  
Vol 86 ◽  
Author(s):  
Anna Karoline Amaral Sousa ◽  
Bruno Raphael Ribeiro Guimarães ◽  
Priscila Alencar Beserra ◽  
Danilo Cutrim Bezerra ◽  
Ferdinan de Almeida Melo ◽  
...  
Keyword(s):  
Risk Factors ◽  
Brucella Abortus ◽  
Bovine Brucellosis ◽  
Blood Samples ◽  
Male And Female ◽  
Specific Antibodies ◽  
Laboratory Results ◽  
Inspection Service ◽  
Serum Agglutination ◽  
Do So

ABSTRACT: The aims of the current study were to investigate the prevalence of bovine brucellosis, to correlate laboratory results of serum reactive samples to the serology of animals presenting serous pouches, and to identify possible risk factors for the development of the disease. In order to do so, 1,265 bovine blood samples were collected from male and female animals bred in different counties in Maranhão state, Brazil, and in other regions of the country. The animals were slaughtered in two slaughterhouses controlled by the Federal Inspection Service (FIS), and in two others controlled by the Municipal Inspection Service (MIS), of regions such as Açailândia and Imperatriz. Samples were tested for specific antibodies by using buffered acidified antigen (BAA) and 2-mercaptoethanol in combination with slow serum agglutination (2-ME + SSA). Additionally, an epidemiological questionnaire was applied to 100 owners, who provided animals to the slaughterhouses, to enable investigating the risk factors for bovine brucellosis. Fifteen serous pouches of animals presenting this lesion were also collected at slaughter time. Thirty-nine out of the analyzed samples were reacted to BAA, whereas 15 reacted to the 2-ME + SSA test; only one male was reagent and it resulted in 1.19% prevalence. One out of the 15 collected serous pouches reacted to the 2-ME + SSA test. The risk factors identified in this study were: the incidence of miscarriages, the sale of animals without previous examination, and the failure in testing the animals before introducing them in herds and before slaughter. It was possible to conclude that the animals slaughtered in the herein evaluated slaughterhouses were infected with Brucella abortus, as well as that this infection prevailed in females.

Evaluation of the combined use of the recombinant Brucella abortus Omp10, Omp19 and Omp28 proteins for the clinical diagnosis of bovine brucellosis

2015 ◽  
Vol 83-84 ◽  
pp. 41-46 ◽  
Author(s):  
Hannah Leah Tadeja Simborio ◽  
Jin Ju Lee ◽  
Alisha Wehdnesday Bernardo Reyes ◽  
Huynh Tan Hop ◽  
Lauren Togonon Arayan ◽  
...  
Keyword(s):  
Clinical Diagnosis ◽  
Brucella Abortus ◽  
Bovine Brucellosis ◽  
Combined Use

scholarly journals eroprevalence of bovine brucellosis in trade cattle slaughtered in Ibadan, Nigeria, from 2004-2006

2010 ◽  
Vol 81 (1) ◽  
Author(s):  
S. I.B. Cadmus ◽  
H. K. Adesokan ◽  
B. O. Adedokun ◽  
J. A. Stack
Keyword(s):  
Brucella Abortus ◽  
Age Distribution ◽  
Bovine Brucellosis ◽  
Southwestern Nigeria ◽  
The Public ◽  
Seroprevalence Study ◽  
Significant Difference ◽  
The Rose ◽  
Ibadan Nigeria

A seroprevalence study was carried out among trade cattle slaughtered at Bodija Municipal Abattoir, Ibadan (southwestern Nigeria) over a period of 3 consecutive years from 2004 to 2006 with a view to determining the breed, sex and age distribution in the seropositivity of bovine brucellosis. In total, 1642 animals were examined for antibodies to Brucella abortus using the Rose Bengal test. Seroprevalences of 6.00 %, 6.17 % and 5.31 % were obtained in the years 2004, 2005 and 2006, respectively but a decrease in 2006 shows no significant difference (P>0.05). The role of the breed (P>0.05), sex (P>0.05) and age (P>0.05) in the occurrence of the infection was not statistically significant at 5 %, although higher rates were obtained for females and older animals. The trend in the disease over the 3-year period showed that it is endemic in trade cattle slaughtered in Ibadan and the public health implications of this are discussed.

scholarly journals Evaluation of Brucella abortus Phosphoglucomutase (pgm) Mutant as a New Live Rough-Phenotype Vaccine

2003 ◽  
Vol 71 (11) ◽  
pp. 6264-6269 ◽  
Author(s):  
Juan Esteban Ugalde ◽  
Diego José Comerci ◽  
M. Susana Leguizamón ◽  
Rodolfo Augusto Ugalde
Keyword(s):  
Brucella Abortus ◽  
Cytokine Production ◽  
Side Chain ◽  
Bovine Brucellosis ◽  
Vaccine Candidates ◽  
Th1 Response ◽  
O Antigen ◽  
Intracellular Multiplication ◽  
Fluorescence Polarization Assay ◽  
Antigen Antibody

ABSTRACT Brucella abortus S19 is the vaccine most frequently used against bovine brucellosis. Although it induces good protection levels, it cannot be administered to pregnant cattle, revaccination is not advised due to interference in the discrimination between infected and vaccinated animals during immune-screening procedures, and the vaccine is virulent for humans. Due to these reasons, there is a continuous search for new bovine vaccine candidates that may confer protection levels comparable to those conferred by S19 but without its disadvantages. A previous study characterized the phenotype associated with the phosphoglucomutase (pgm) gene disruption in Brucella abortus S2308, as well as the possible role for the smooth lipopolysaccharide (LPS) in virulence and intracellular multiplication in HeLa cells (J. E. Ugalde, C. Czibener, M. F. Feldman, and R. A. Ugalde, Infect. Immun. 68:5716-5723, 2000). In this report, we analyze the protection, proliferative response, and cytokine production induced in BALB/c mice by a Δpgm deletion strain. We show that this strain synthesizes O antigen with a size of approximately 45 kDa but is rough. This is due to the fact that the Δpgm strain is unable to assemble the O side chain in the complete LPS. Vaccination with the Δpgm strain induced protection levels comparable to those induced by S19 and generated a proliferative splenocyte response and a cytokine profile typical of a Th1 response. On the other hand, we were unable to detect a specific anti-O-antigen antibody response by using the fluorescence polarization assay. In view of these results, the possibility that the Δpgm mutant could be used as a vaccination strain is discussed.

scholarly journals Investigating the Use of Protein Saver Cards for Storage and Subsequent Detection of Bovine Anti-Brucella abortus Smooth Lipopolysaccharide Antibodies and Gamma Interferon

2013 ◽  
Vol 20 (11) ◽  
pp. 1669-1674 ◽  
Author(s):  
Lucy Duncombe ◽  
Nicola J. Commander ◽  
Sevil Erdenlig ◽  
John A. McGiven ◽  
Judy Stack
Keyword(s):  
Blood Serum ◽  
Brucella Abortus ◽  
Room Temperature ◽  
Plasma Samples ◽  
Bovine Blood ◽  
Bovine Brucellosis ◽  
Serum Samples ◽  
Content Type ◽  
Antibody Titers ◽  
Ifn Γ

ABSTRACTBrucella abortus, a smooth strain of the genusBrucella, is the causative agent of bovine brucellosis. To support the ongoing development of diagnostic tests for bovine brucellosis, the use of Protein Saver cards (Whatman) for bovine blood serum and plasma sample collection has been evaluated. These cards offer significant logistical and safety alternatives to transporting and storing liquid samples and may aid in diagnostic programs and validation studies. To evaluate the utility of these cards, 204 bovine blood serum samples fromBrucella-infected and noninfected animals were stored on and eluted from the Protein Saver cards. Anti-Brucellasmooth lipopolysaccharide (sLPS) antibody titers for the serum eluates were compared to those of the unprocessed original serum samples by indirect enzyme-linked immunosorbent assay (ELISA). The results showed a highly significant correlation between titers from the serum eluates and the unprocessed sera. Therefore, under these circumstances, serum eluates and unprocessed serum samples may be used interchangeably. Blood plasma from 113 mitogen-stimulated whole-blood samples was added to and eluted from the Protein Saver cards. The gamma interferon (IFN-γ) titers in the plasma eluates were compared to those of the unprocessed plasma samples obtained by IFN-γ ELISA. The results showed a significant correlation between the plasma eluates and the unprocessed plasma samples. To derive a signal in the plasma eluate, it was necessary to develop a novel and highly sensitive ELISA for the detection of IFN-γ. The serum samples stored on cards at room temperature over a 10-day period showed little variation in antibody titers. However, the plasma eluates showed a progressive loss of IFN-γ recovery over 10 days when stored at room temperature.

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