Entrapment of calf-thymus DNA on magnetic nanoparticles

Magnetic nanoparticles can be used to load bio–molecules for various biophysical applications. The direct attachment of bio–molecules such as protein and DNA to magnetic nanoparticles may lead to alter their structure. A method to immobilize and store biomolecules for example DNA on to a magnetic nanoparticles surface without much structural alteration is carried out in the present work. DNA is a target for many therapeutic small molecules as therapeutic drugs bind to DNA – interfering with protein factors involved in DNA mechanism, or cleave DNA cross-link – interfering with the cell division. This may find applications in drug interactions study with the stored DNA without much alteration in their structure.

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Review on the Clinical Pharmacology of Hydroxychloroquine Sulfate for the Treatment of COVID-19

Current Drug Metabolism ◽

10.2174/1389200221666200610172929 ◽

2020 ◽

Vol 21 (6) ◽

pp. 427-435 ◽

Cited By ~ 1

Author(s):

Cheng Cui ◽

Siqi Tu ◽

Valerie Sia Jie En ◽

Xiaobei Li ◽

Xueting Yao ◽

...

Keyword(s):

Drug Interactions ◽

Clinical Efficacy ◽

Relevant Literature ◽

The United States ◽

Efficacy And Safety ◽

Open Label ◽

Infected People ◽

Treatment Regimens ◽

Therapeutic Drugs

Background: As the number of severe acute respiratory syndrome coronavirus 2 (SARS-COV-2) infected people is greatly increasing worldwide, the international medical situation becomes very serious. Potential therapeutic drugs, vaccine and stem cell replacement methods are emerging, so it is urgent to find specific therapeutic drugs and the best treatment regimens. After the publications on hydroxychloroquine (HCQ) with anti- SARS-COV-2 activity in vitro, a small, non-randomized, open-label clinical trial showed that HCQ treatment was significantly associated with reduced viral load in patients with coronavirus disease-19 (COVID-19). Meanwhile, a large prophylaxis study of HCQ sulfate for COVID-19 has been initiated in the United States. HCQ offered a promising efficacy in the treatment of COVID-19, but the optimal administration is still being explored. Methods: We used the keyword "hydroxychloroquine" to conduct a literature search in PubMed to collect relevant literature on the mechanism of action of HCQ, its clinical efficacy and safety, pharmacokinetic characteristics, precautions for clinical use and drug interactions to extract and organize information. Results: This paper reviews the mechanism, clinical efficacy and safety, pharmacokinetic characteristics, exposureresponse relationship and precautions and drug interactions of HCQ, and summarizes dosage recommendations for HCQ sulfate. Conclusion: It has been proved that HCQ, which has an established safety profile, is effective against SARS-CoV-2 with sufficient pre-clinical rationale and evidence. Data from high-quality clinical trials are urgently needed worldwide.

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Molecular Transformations in DNA under the Influence of UV-radiation

Current Applied Polymer Science ◽

10.2174/2452271604666200814123505 ◽

2020 ◽

Vol 04 ◽

Author(s):

Vigen G. Barkhudaryan ◽

Gayane V. Ananyan ◽

Nelli H. Karapetyan

Keyword(s):

Uv Radiation ◽

Uv Irradiation ◽

Absorption Spectroscopy ◽

Calf Thymus Dna ◽

Dilute Solutions ◽

Concentrated Solutions ◽

Buffer Solution ◽

Low Concentration ◽

Calf Thymus ◽

Dna Solutions

Background: The processes of destruction and crosslinking of macromolecules occur simultaneously under the influence of ultraviolet (UV) radiation in synthetic polymers, dry DNA and their concentrated solutions. Objective: The effect of UV radiation on calf thymus DNA in dilute solutions subjected to UV- irradiation was studied in this work. Method: The calf thymus DNA was studied in dilute solutions using viscometry, absorption spectroscopy and electrophoresis. Results: It was shown, that at a low concentration of DNA in the buffer solution ([DNA] = 85 μg / ml) under the influence of UV radiation, the processes of destruction of macromolecules and an increase in their flexibility predominate, which is accompanied by a gradual decrease in the viscosity of their solution. In addition, due to the low concentration of the solution, intramolecular crosslinking of macromolecules predominates, which also reduces their size and, consequently, the viscosity of the solution. Conclusion: It was concluded, that in dilute DNA solutions, due to the predominance of the processes of intramolecular crosslinking of macromolecules over intermolecular, only constant processes of decreasing the sizes of DNA macromolecules occur. As a result, its solubility remains virtually unchanged during UV irradiation. The described comments are also excellently confirmed by the results of absorption spectroscopy and electrophoresis

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Ce(IV)-mediated formation of benzenediazonium ion from a non-aminoazo dye, 1-phenylazo-2-hydroxy-naphthalene (Sudan I) and its binding to DNA

Collection of Czechoslovak Chemical Communications ◽

10.1135/cccc19892021 ◽

1989 ◽

Vol 54 (7) ◽

pp. 2021-2026

Author(s):

Marie Stiborová ◽

Befekadu Asfaw ◽

Pavel Anzenbacher

Keyword(s):

Azo Dyes ◽

Acidic Medium ◽

Model System ◽

Calf Thymus Dna ◽

Suitable Model ◽

Principal Product ◽

Calf Thymus ◽

Sudan I ◽

A Minor

Ce(IV) ions in acidic medium convert a carcinogenic non-aminoazo dye, 1-phenylazo-2-hydroxy-naphthalene (Sudan I) into an ultimate carcinogen, which binds to calf thymus DNA. The principal product of Sudan I oxidation by the Ce(IV) system is the benzenediazonium ion. A minor product is the dihydroxyderivative of Sudan I, 1-(4-hydroxyphenylazo)-2,6-dihydroxynaphthalene. Other minor coloured products (yellow and brown) were not identified. The principal product (the benzenediazonium ion) is responsible for the carcinogenicity of Sudan I, as it covalently binds to DNA. Ce(IV) ions in acidic medium represent a suitable model system, which imitates the activation route of carcinogenic azo dyes.

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