scholarly journals Antioxidant Potential of Chestnut Shell, Stinging Nettle, Kiwi Fruit and Citrus Fruit Extracts and Antimicrobial Effects Against Some Fish Pathogens

Author(s):  
Orhan KOBYA ◽  
Büşra KARA ◽  
Ecren UZUN YAYLACI ◽  
Emre ÇAĞLAK
2019 ◽  
pp. 27-32
Author(s):  
Emőke Mihok ◽  
Éva György ◽  
Endre Máthé

Wild berry is an excellent source of phytonutrients and/or bioactive compounds associated with significant therapeutic properties, so that they have been utilized in folk medicine and traditional nutrition throughout centuries. Multiple health-promoting effects, such as anti-inflammatory, anti-diabetic, anti-heart and coronary disease properties were attributed to such wild berries. It has also been proved that berries could feature antimicrobial effects that could be of a great importance for the prevention of food-feed poisoning and fighting back antibiotic resistance. In this study, we investigated the antimicrobial properties of lingonberry (Vaccinium vitis-idaea), raspberry (Rubus idaeus) and blackberry (Rubus fruticosus) crude and ethanolic extracts prepared from fruits obtained from the spontaneous flora of Eastern Carpathian Mountains situated in Transylvania. The antimicrobial effect of crude and alcoholic extracts were assessed on four Gram-negative, five Gram-positive bacteria and one yeast species using the agar diffusion method. The studied bacteria can cause food or feed spoilage and foodborne diseases. Our results indicate the significant inhibitory effect of lingonberry extracts in the case of Gram-negative bacteria like Proteus vulgaris and Salmonella Hartford, while among Gram-positive bacteria the strongest inhibitory effect was observed for Bacillus species like B. cereus, B. subtilis, B. mojavensis and Micrococcus luteus. The raspberry and blackberry extracts featured milder inhibitory effects in the case of the studied bacteria species. Furthermore, we have studied the crude or ethanolic extract combinations associated antimicrobial effects synergistic/additive or antagonistic properties. Interestingly, the triple and double ethanolic extract mixes had stronger antimicrobial properties, whereas the crude extract mixes showed relatively reduced effects, if any. Our results indicate that the antimicrobial activity of studied fruit extracts obtained from wild berries can vary upon the applied extraction method and their combination formulae, so that all these considerations must be taken into account when such fruit extracts are considered for foodstuff development.


2010 ◽  
Vol 58 (20) ◽  
pp. 11119-11129 ◽  
Author(s):  
Deena Ramful ◽  
Evelyne Tarnus ◽  
Philippe Rondeau ◽  
Christine Robert Da Silva ◽  
Theeshan Bahorun ◽  
...  

2009 ◽  
Vol 57 (4) ◽  
pp. 1565-1571 ◽  
Author(s):  
M. Antonietta Ciardiello ◽  
Ivana Giangrieco ◽  
Lisa Tuppo ◽  
Maurizio Tamburrini ◽  
Marina Buccheri ◽  
...  

Author(s):  
Geetika Sharma ◽  
Vipasha Sharma ◽  
Tulika Mishra

Objective: The main objective of this study was to determine the enhancement of in vitro antioxidant potential of fruits of Terminalia chebula (TC) when used in combination with fruit extracts of Phyllanthus emblica, Ananas comosus, and Punica granatum. Hydroxyl (OH) radical scavenging and 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging assays were used to analyze the antioxidant potential.Method: Formulations of different combinatorial concentrations of fruits to prepare a mixture were achieved with central composite design through response surface methodology. Screening of 300 different combinations of various concentrations was done through hydroxyl radical scavenging assay followed by statistical analysis of data. Further validation of results was done by measuring the antioxidant potential of most bioactive extracts by DPPH method.Results: Screening of 300 samples of different combinations for antioxidant potential revealed the samples with highest percentage inhibition in aqueous (85.2%), ethanolic (92.9%), and aqueous-ethanolic (84.21%) extracts. Data were subjected to analysis of variance and generated a three-dimensional response surface plot for highest activity. Further subjecting these extracts to DPPH assay revealed a significant enhancement in the antioxidant potential of ethanolic extract of TC when used in mixture with other plants.Conclusion: Antioxidant activity of TC was enhanced when used in combination with other fruits extracts. These synergistic studies generating valuable interactions between various phytochemicals could lead to a momentous increase in other associated activities to fight against diseases such as cancer and cardiovascular disorders. Further research on isolation of bioactive compounds in the mixture and their potential to fight various types of cancer could lead to a significant augmentation in the activity of natural compounds.


Author(s):  
Irda Fidrianny ◽  
Siti Winarsih ◽  
Komar Ruslan

  Objectives: The goals of this research were to evaluate antioxidant potential from different organs of eggplant (Solanum melongena L.) using two antioxidant testing methods which were 2,2-diphenyl-1-picrylhydrazyl (DPPH) and ferric reducing antioxidant power (FRAP) and correlation of total phenolic and flavonoid content with their inhibitory concentration 50% (IC50) of DPPH, and exhibitory concentration 50% (EC50) of FRAP.Materials and Methods: Each sample was extracted by reflux using different polarity solvents. The extracts were evaporated using rotary evaporator. Antioxidant activities were tested using DPPH and FRAP assays, determination of total phenolic and flavonoid content were carried out by ultraviolet-visible spectrophotometry and correlation with their IC50 of DPPH and EC50 of FRAP capacities were analyzed by Pearson’s method.Results: The lowest IC50 of DPPH scavenging activity 1.14 μg/ml and the lowest EC50 of FRAP capacity 49.80 μg/ml was given by ethanolic leaves extract of eggplant. Ethanolic leaves extract of eggplant also presented the highest total phenolic content (TPC) (8.87 g gallic acid equivalent/100 g), while the highest total flavonoid content was shown by ethyl acetate leaves extract (24.50 g quercetin equivalent/100 g). There was a significantly negative correlation between TPC in leaves and fruit extracts of eggplant with their IC50 of DPPH and EC50 of FRAP.Conclusions: All different extracts of eggplant organs (except n-hexane stem extract) were categorized as a very strong antioxidant by DPPH method. Phenolic compounds in eggplant leaves and fruit extracts were the major contributor in antioxidant activities by DPPH and FRAP methods. DPPH and FRAP showed linear results in antioxidant activities of eggplant leaves, fruit and stem extracts.


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