scholarly journals ENHANCEMENT OF IN VITRO ANTIOXIDANT POTENTIAL OF TERMINALIA CHEBULA BY VARIOUS FRUIT EXTRACTS AND OPTIMIZATION OF CONCENTRATION BY RESPONSE SURFACE METHODOLOGY

2018 ◽  
Vol 11 (7) ◽  
pp. 228
Author(s):  
Geetika Sharma ◽  
Vipasha Sharma ◽  
Tulika Mishra
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Radical Scavenging ◽  
Ethanolic Extract ◽  
Punica Granatum ◽  
Antioxidant Potential ◽  
Ananas Comosus ◽  
Terminalia Chebula ◽  
Fruit Extracts

Objective: The main objective of this study was to determine the enhancement of in vitro antioxidant potential of fruits of Terminalia chebula (TC) when used in combination with fruit extracts of Phyllanthus emblica, Ananas comosus, and Punica granatum. Hydroxyl (OH) radical scavenging and 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging assays were used to analyze the antioxidant potential.Method: Formulations of different combinatorial concentrations of fruits to prepare a mixture were achieved with central composite design through response surface methodology. Screening of 300 different combinations of various concentrations was done through hydroxyl radical scavenging assay followed by statistical analysis of data. Further validation of results was done by measuring the antioxidant potential of most bioactive extracts by DPPH method.Results: Screening of 300 samples of different combinations for antioxidant potential revealed the samples with highest percentage inhibition in aqueous (85.2%), ethanolic (92.9%), and aqueous-ethanolic (84.21%) extracts. Data were subjected to analysis of variance and generated a three-dimensional response surface plot for highest activity. Further subjecting these extracts to DPPH assay revealed a significant enhancement in the antioxidant potential of ethanolic extract of TC when used in mixture with other plants.Conclusion: Antioxidant activity of TC was enhanced when used in combination with other fruits extracts. These synergistic studies generating valuable interactions between various phytochemicals could lead to a momentous increase in other associated activities to fight against diseases such as cancer and cardiovascular disorders. Further research on isolation of bioactive compounds in the mixture and their potential to fight various types of cancer could lead to a significant augmentation in the activity of natural compounds.

scholarly journals IN VITRO EVALUATION OF ANTIOXIDANT-MEDIATED APOPTOSIS ON CANCER CELLS AND NON-TOXICITY OF ETHANOLIC EXTRACT OF TERMINALIA CHEBULA RETZ AND PYROGALLOL

2019 ◽  
Vol 12 (1) ◽  
pp. 212
Author(s):  
Deena Priscilla H ◽  
Jasmine R
Keyword(s):  
Research Work ◽  
Ethanolic Extract ◽  
Antioxidant Potential ◽  
Oxygen Species ◽  
Terminalia Chebula ◽  
Inhibition Assay ◽  
Hemolysis Assay ◽  
Anticancer Properties ◽  
Lipid Peroxidation Inhibition

Objective: Antioxidants are very important for human health, and hence, external supplementation is recommended to protect cells from the deleterious effects of excessive reactive oxygen species conditions that are capable of causing direct damage to deoxyribonuclieic acid (DNA) which might lead to the cause of cancer. In our research work, we have aimed to evaluate the antioxidant potential of Terminalia chebula and pyrogallol to relate to its apoptotic nature as well as prove its non-toxicity.Methods: Investigations were carried out to determine the antioxidant activity by 2,2-diphenyl-1-picrylhydrazyl and lipid peroxidation inhibition assay of the ethanolic extract of T. chebula and pyrogallol. The apoptotic nature of tumor cells was determined by comet assay. To prove the non-toxicity of the extract as well as compound, the hemolysis assay was carried out.Results: The results of our study established the antioxidant potential of the plant which may serve as additional evidence for the antiproliferative activity of the plant. It is considered safe for human use.Conclusion: Plant-based traditional medicine system continues to play an essential role in health care. The ethanolic extract of T. chebula and pyrogallol possesses antioxidant and anticancer properties.

scholarly journals Effects of Time, Temperature, and Solvent Ratio on the Extraction of Non-Extractable Polyphenols with Anticancer Activity of Barhi Date Palm Kernels Extracts Using Response Surface Methodology

2019 ◽  
Author(s):  
Israa Mahmud ◽  
Mohamed E. S. Mirghani ◽  
Faridah Yusof ◽  
Ma'an Al-khatib
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Date Palm ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Solvent Ratio ◽  
Extraction Temperature ◽  
Dietary Polyphenols ◽  
Experimental Values

Dietary polyphenols exist in two forms; extractable polyphenols (EPP) or compounds solubilised by aqueous/organic solvents, and non-extractable polyphenols (NEPP) or compounds remain in the corresponding residues after the extraction. At present, most researchers focus on EEP fractions, while NEPP is neglected. Thus, this study aimed to release NEPP from the remaining powder residue of Barhi date palm kernels (BDPK) with acid hydrolysis. The related extraction conditions were determined and optimised using response surface methodology (RSM) for maximisation of NEPP with highest cytotoxic and antioxidant activities. The face-centred central composite design (FCCCD) was used to establish treatments based on three independent variables, namely; extraction temperature, time, and solvent/sample ratio. Under the optimal conditions, the experimental values for DPPH radical-scavenging capacity of NEPP (IC50=57.52µg/mL), and cytotoxicity of NEPP against A549 and HT29 cells were IC50=17.4 µg/mL and 31.4µg/mL, respectively. The experimental values were in agreement with those predicted by RSM models, confirming the suitability of the model employed and the success of RSM for optimisation of the extraction conditions for NEPP from BDPK. These results indicate that NEPP from industrial date fruit waste could be a promising candidate as natural antioxidants with significant antiproliferation effect against A549 and HT29 cancer cells in-vitro.

Characterization and Antioxidant Activity of Flash-Assisted Extracted Dihydroquercetin from Wood Sawdust of Larix gmelinii Using a Response Surface Methodology

2016 ◽  
Vol 12 (6) ◽  
pp. 587-597 ◽  
Author(s):  
Jianxia Li ◽  
Qiaohui Zhang ◽  
Jie Cui ◽  
Yaping Ning ◽  
Fengjun Wang ◽  
...  
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Larix Gmelinii ◽  
Wood Sawdust ◽  
Assisted Extraction

Abstract We investigate a flash-assisted extraction of dihydroquercetin (DHQ) from wood sawdust of Larix gmelinii by response surface methodology. Box–Behnken design optimized the extraction conditions, and the highest DHQ yield (6.15 %) was obtained under optimal extraction conditions: FAE time (3 min), extraction time (3 h), solid-to-liquid (g/mL) ratio (1:15), and ethanol concentration (75 %). After purification, the DHQ purity was 96 % as determined by HPLC and was further characterized by FTIR. Purified DHQ’s antioxidant activities were confirmed in vitro by determining DPPH and ABTS radical scavenging activities and reducing Fe3+ power. In the DPPH and ABTS radical scavenging activity assays, DHQ displayed prominent antioxidant activities with low IC50 values (11.568 and 12.475 μg/mL, respectively). In reducing Fe3+ power assay, high DHQ absorbance values showed that DHQ had higher reducing power than butylated hydroxyl toluene and had nearly the same consistent power as ascorbic acid at the same doses.

scholarly journals IN VITRO EVALUATION OF ANTIOXIDANT-MEDIATED APOPTOSIS ON CANCER CELLS AND NON-TOXICITY OF ETHANOLIC EXTRACT OF TERMINALIA CHEBULA RETZ AND PYROGALLOL

2019 ◽  
Vol 12 (1) ◽  
pp. 212
Author(s):  
Deena Priscilla H ◽  
Jasmine R
Keyword(s):  
Research Work ◽  
Ethanolic Extract ◽  
Antioxidant Potential ◽  
Oxygen Species ◽  
Terminalia Chebula ◽  
Inhibition Assay ◽  
Hemolysis Assay ◽  
Anticancer Properties ◽  
Lipid Peroxidation Inhibition

Objective: Antioxidants are very important for human health, and hence, external supplementation is recommended to protect cells from the deleterious effects of excessive reactive oxygen species conditions that are capable of causing direct damage to deoxyribonuclieic acid (DNA) which might lead to the cause of cancer. In our research work, we have aimed to evaluate the antioxidant potential of Terminalia chebula and pyrogallol to relate to its apoptotic nature as well as prove its non-toxicity.Methods: Investigations were carried out to determine the antioxidant activity by 2,2-diphenyl-1-picrylhydrazyl and lipid peroxidation inhibition assay of the ethanolic extract of T. chebula and pyrogallol. The apoptotic nature of tumor cells was determined by comet assay. To prove the non-toxicity of the extract as well as compound, the hemolysis assay was carried out.Results: The results of our study established the antioxidant potential of the plant which may serve as additional evidence for the antiproliferative activity of the plant. It is considered safe for human use.Conclusion: Plant-based traditional medicine system continues to play an essential role in health care. The ethanolic extract of T. chebula and pyrogallol possesses antioxidant and anticancer properties.

scholarly journals STUDIES ON NUTRITIONAL PROFILE AND ANTIOXIDANT POTENTIAL OF DIFFERENT DIOSCOREA SP WITH PLECTRANTHUS ROTUNDIFOLIUS

2017 ◽  
Vol 9 (4) ◽  
pp. 65 ◽  
Author(s):  
Jenit K. Joy ◽  
P. Siddhuraju
Keyword(s):  
Nutritive Value ◽  
Radical Scavenging Activity ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Ethanolic Extract ◽  
Antioxidant Potential ◽  
Nutritional Requirement ◽  
Health Food ◽  
Antioxidant Defence System

Objective: Protection from oxidative damage to the tissues is provided by natural or synthetic antioxidants. Because of the multiple mechanisms of actions and presence of various phytochemicals, plant-based antioxidants are preferred. Dioscorea is well known as a traditional edible tuber. The present study was undertaken to explore and highlights the nutritional benefits and to evaluate the in vitro antioxidant and free radical scavenging capacity of ethanolic extract of three different underutilised Dioscorea species (D. alata, D. pentaphylla and D. oppositifolia) with a common cultivated crop, Plectranthus rotundifolius.Methods: Different processing methods are adopted to assess their influence on nutritive value and antioxidant properties. Tubers were studied for proximate composition using standard analytical methods. Mineral elements were analysed using ICP-MS (Inductively Coupled Plasma-Mass Spectrometer). Antioxidant potential of raw and processed samples was determined using a range of in vitro models.Results: The presence of essential minerals such as Na, K, Ca, P, Zn and Mn were observed. High radical scavenging activity was observed in the tubers of Dioscorea. Results indicated that phenolic compounds are the significant contributors to the antioxidant activity.Conclusion: Current study suggests that Dioscorea might be a promising natural antioxidant and could be very useful health food, as they enhance the antioxidant defence system. It also proposes that underutilized tubers can efficiently contribute to the nutritional requirement and food security. 

scholarly journals Qualitative and Quantitative Determination of Secondary Metabolites and Antioxidant Potential of Plumbago indica Root Extracts

2021 ◽  
pp. 319-325
Author(s):  
Kavita Mane ◽  
Rajendra Patil ◽  
Aditya Nath Pandey
Keyword(s):  
Ascorbic Acid ◽  
Radical Scavenging ◽  
Ethanolic Extract ◽  
Antioxidant Potential ◽  
Total Phenolic ◽  
Accurate Identification ◽  
Test Technique ◽  
Phytochemical Study ◽  
Plumbago Indica

Due to their well-known attribute of having minimal side effects as compared to medicines, natural items with medical potential are progressively gaining prominence in clinical research. The roots of Plumbago indica (P. indica, Plumbaginaceae) are commercially significant since they are the primary source of plumbagin and its derivatives. Plumbagin is well-known for its many pharmacological properties. P. indica roots yielded three naphthoquinones: plumbagin, 3, 30-biplumbagin, and elliptinone, which were employed as standard markers for quantitative HPLC analysis. The goal of this research was to screen phytochemicals, assess alkaloids, phenolic and flavonoid content, and measure the antioxidant potential of P. indica roots. The well-known test methodology was used to determine qualitative analysis of several phytochemical ingredients as well as quantitative analysis of total alkaloids, phenol, and flavonoids. The antioxidant activity of an ethanolic extract of P. indica roots was investigated in vitro using the 1,1-diphenyl, 2-picrylhydrazyl (DPPH) test technique. Alkaloids, glycosides, flavonoids, saponins, phenolics, proteins, and glucose were found in the ethanol and aqueous extracts, according to phytochemical study. P. indica roots ethanolic extract had 5.55, 0.930, and 3.940 mg of total phenolic, flavonoids, and alkaloids respectively. For comparative purposes, ascorbic acid was employed as a benchmark. In the investigated models, the extract showed dose-dependent free radical scavenging properties. For the DPPH technique, P. indica roots extract had an IC50 value of 23.02 µg/ml, which was equivalent to that of ascorbic acid (IC50=17.68 µg/ml). These researches contributed to the accurate identification of this plant material. The plant's broad variety of phytochemicals implies that it has medicinal potential, which might be investigated in the pharmaceutical sector as well as in traditional medicine.

scholarly journals Antioxidant and antibacterial activities of fruit extracts of Berberis species from Nepal

2016 ◽  
Vol 10 ◽  
pp. 6-11
Author(s):  
Sarala Dhungel ◽  
Giri Prasad Joshi ◽  
Deepak Raj Pant
Keyword(s):  
Radical Scavenging ◽  
Antioxidant Property ◽  
Antibacterial Activities ◽  
Antioxidant Potential ◽  
Total Phenolic ◽  
Phytochemical Analysis ◽  
Bacterial Strains ◽  
High Concentration ◽  
Fruit Extracts

  The Himalayan region is rich in flowering plants diversity, including medicinal and wild edible plants. These plants possess variety of therapeutically important compounds, like alkaloids, flavonoids, phenolics, saponins, steroids, tannins and terpenoids in different parts. Presence of significant amount of these compounds makes a species valuable to be used as super foods or medicines. Furthermore, plant extracts especially those of fruits are basically attributed with natural antioxidants, either in form of vitamins or color-inducing pigments, which have become the target to replace the synthetic antioxidants. In this regard, fruits of Berberis species, one of the popular wild edible fruits of Nepal, are noted for their antioxidant property since time immemorial. The present study was done with an attempt to quantify the antioxidant potential and antibacterial activities of the fruit extracts of four taxa of Berberis (B. angulosa var. angulosa, B. angulosa var. fasciculata, B. aristata and B. asiatica) from Nepal and to correlate the antioxidant potential with various phytochemicals present in the extracts. Methanolic fruit extracts were used to spectrophotometrically quantify total phenolic and flavonoid contents. DPPH free radical scavenging assay and antibacterial assay were carried out in in-vitro condition. Preliminary phytochemical analysis revealed high polyphenol content (52.60 ± 3.73 and 58.07 ± 1.44 mg GAE/g) and better antioxidant property (35.29 ± 3.01 and 29.15 ± 2.01 μg/ml) respectively in B. angulosa var. angulosa and B. angulosa var. fasciculata than in the fruit extracts of B. aristata and B. asiatica. In contrast, fruit extracts of B. asiatica showed the highest total flavonoid content (27.52 ± 0.56 mg QE/g) than did by the extracts of other taxa studied. The fruit extract of B. aristata and B. angulosa var. fasciculata at very high concentration (200 mg/ml) showed maximum zone of inhibition (ZOI) against tested bacterial strains, Staphylococcus aureus and Escherichia coli. In contrast, B. asiatica did not show any ZOI for both of the tested bacterial strains. As fruits are better sources of antioxidant with greater accumulation of flavonoids and phenolics, wild fruits should be equally spaced for their better efficiency as cultivated ones.Botanica Orientalis – Journal of Plant Science (2016) 10: 6–11

Synthesis, Characterization, Antimicrobial and Antioxidant Potential of Diazenyl Chalcones

2018 ◽  
Vol 18 (10) ◽  
pp. 844-856 ◽  
Author(s):  
Harmeet Kaur ◽  
Balasubramanian Narasimhan
Keyword(s):  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Antioxidant Potential ◽  
Dilution Method ◽  
Dpph Assay ◽  
Antimicrobial Potential ◽  
Structural Conformation ◽  
Uv Visible ◽  
Tube Dilution Method

A series of diazenyl chalcones was prepared by base catalyzed Claisen-Schmidt condensation of synthesized hydroxy substituted acetophenone azo dye with various substituted aromatic/ heteroaromatic aldehydes. The structural conformation of synthesized chalcones was done by a number of physicochemical and spectral means like FTIR, UV-visible, mass, NMR spectroscopy and CHNS/O analysis. These diazenyl chalcones were assessed for their in vitro antimicrobial potential against several Gram-negative, Gram-positive bacterial and fungal strains by serial tube dilution method. The fluconazole and cefadroxil were used as standard drugs. The target compounds were also evaluated for their antioxidant potential by DPPH assay. (2E)-3-(2,4-Dichlorophenyl)-1-(4-((2,6- dihydroxyphenyl)diazenyl)phenyl)prop-2-en-1-one (C-7) had shown very good antimicrobial potential with MIC ranges from 3.79 to 15.76 μg/ml against most of the tested microorganisms. Most of the synthesized diazenyl chalcones were found to be active against B. subtilis. The (2E)-1-(5-((2-Chloro- 4-nitrophenyl)diazenyl)-2-hydroxyphenyl)-3-(2-hydroxynaphthalen-1-yl)prop-2-en-1-one (C-10) had shown high free radical-scavenging activity when compared with the ascorbic acid as the reference antioxidant.

Synthesis and antioxidant activity of new selenium-containing quinolines

2020 ◽  
Vol 16 ◽  
Author(s):  
Benedetta Bocchini ◽  
Bruna Goldani ◽  
Fernanda S.S. Sousa ◽  
Paloma T. Birmann ◽  
Cesar A. Brüning ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Superoxide Dismutase ◽  
Radical Scavenging ◽  
Building Blocks ◽  
Antioxidant Potential ◽  
In Vitro Assays ◽  
Pharmacological Activities ◽  
Antioxidant Power ◽  
Antioxidant Agents

Background: Quinoline derivatives have been attracted much attention in drug discovery and synthetic derivatives of these scaffolds present a range of pharmacological activities. Therefore, organoselenium compounds are valuable scaffolds in organic synthesis because their pharmacological activities and their use as versatile building blocks for regio-, chemio-and stereoselective reactions. Thus, the synthesis of selenium-containing quinolines has great significance, and their applicability range from simple antioxidant agents, to selective DNA-binding and photocleaving agents. Objective: In the present study we describe the synthesis and antioxidant activity in vitro of new 7-chloroN(arylselanyl)quinolin-4-amines 5 by the reaction of 4,7-dichloroquinoline 4 with (arylselanyl)-amines 3. Methods: For the synthesis of 7-chloro-N(arylselanyl)quinolin-4-amines 5, we performed the reaction of (arylselanyl)- amines 3 with 4,7-dichloroquinoline 4 in the presence of Et3N at 120 °C in a sealed tube. The antioxidant activities of the compounds 5 were evaluated by the following in vitro assays: 2,2- diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity, 2,2-azinobis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS), ferric ion reducing antioxidant power (FRAP), nitric oxide (NO) scavenging and superoxide dismutase-like activity (SOD-Like). Results: 7-Chloro-N(arylselanyl)quinolin-4-amines 5a-d has been synthesized in yields ranging from 68% to 82% by the reaction of 4,7-dichloroquinoline 4 with arylselanyl-amines 3a-d using Et3N as base, at 120 °C, in a sealed tube for 24 hours and tolerates different substituents, such as -OMe and -Cl, in the arylselanyl moiety. The obtained compounds 5a-d presented significant results with respect to the antioxidant potential, which had effect in the tests of inhibition of radical’s DPPH, ABTS+ and NO, as well as in the test that evaluates the capacity (FRAP) and in the superoxide dismutase-like activity assay (SOD-Like). It is worth mentioning that 7-chloro-N(arylselanyl)quinolin-4-amine 5b presented excellent results, demonstrating a better antioxidant capacity when compared to the others. Conclusion: According to the obtained results 7-chloro-N(arylselanyl)quinolin-4-amines 5 were synthesized in good yields by the reaction of 4,7-dichloroquinoline with arylselanyl-amines and tolerates different substituents in the arylselanyl moiety. The tested compounds presented significant antioxidant potential in the tests of inhibition of DPPH, ABTS+ and NO radicals, as well as in the FRAP and superoxide dismutase-like activity assays (SOD-Like).

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