Efficacy Study of Cypress Leaf Extract Active Ingredient Using High-pressure Hot Water Extraction Method

2021 ◽  
Vol 3 (1) ◽  
pp. 3-10
Author(s):  
Yuri Kang ◽  
◽  
Woonjung Kim
2016 ◽  
Vol 7 (6) ◽  
pp. 2886-2897 ◽  
Author(s):  
Chun Chen ◽  
Bin Zhang ◽  
Xiong Fu ◽  
Rui Hai Liu

A novel polysaccharide (MFP3P) was isolated from Murus alba L. through the hot water extraction method followed by chromatographic purification.


2011 ◽  
Vol 145 ◽  
pp. 154-158
Author(s):  
Pei Sheng Yan ◽  
Li Ya Ma ◽  
Li Xin Cao

Polysaccharides extracted from fruiting body and mycelia of higher fungi have been found to show various biological activities. Traditionally, these polysaccharides are manufactured by the hot water extraction method. Low polysaccharide yield is the disadvantage of this method. This paper reports the optimal conditions to manufacture polysaccharide with high-yield from Hypsizigus marmoreus mycelia using enzymatic hydrolytic extraction method. Neutral protease was selected as the most cost efficient among six enzymes. The uniform design was further conducted to optimize the enzymatic hydrolytic extraction conditions, and a regression model was constructed to predict polysaccharide yield. The optimized procedures was that 2% (w/w) of neutral protease was added into hydrolytic solution and incubated for 4 h at 43°C, then extracted one time at 100°C for 1 h. Under optimized procedures, the measured polysaccharide yield was 15.73±0.15 (mg/g), which was very close to the predicted value of 16.65 (mg/g). The results validated the accuracy of the regression model and the optimized procedure. When following the optimized procedures, polysaccharide yield was raised 75.0% over that before optimization, and increased by 122.5% and 104.8% over hot-water extraction and microwave assisted extraction methods respectively. This was the first study to apply uniform design for optimizing high yield manufacture of mycelia polysaccharides by enzymatic hydrolytic extraction. We concluded that enzymatic hydrolytic extraction was a simple, high yield method to manufacture polysaccharides from mushroom mycelia and might be used widely in polysaccharide-derived functional foods production.


2015 ◽  
Vol 6 (5) ◽  
pp. 1557-1567 ◽  
Author(s):  
Chao Li ◽  
Lijun You ◽  
Xiong Fu ◽  
Qiang Huang ◽  
Shujuan Yu ◽  
...  

A new heteropolysaccharide, here called P1, was isolated from the fruit clusters of Prunella vulgaris using a hot water extraction method.


2015 ◽  
Vol 24 (2) ◽  
pp. 507-512 ◽  
Author(s):  
Jong-Hyun Jin ◽  
Ei-Hyun Chun ◽  
Ju Hwan Hyun ◽  
Sung-Won Choi ◽  
Seung-Tae Su ◽  
...  

2019 ◽  
Vol 10 (6) ◽  
pp. 3150-3160 ◽  
Author(s):  
Jiaqi Zhang ◽  
Chun Chen ◽  
Xiong Fu

Mulberry fruit polysaccharide (MFP) was obtained from Morus alba L. by a hot water extraction method, and mulberry polysaccharide fractions named MFP1, MFP2 and MFP3 were isolated by DEAE cellulose-52 column chromatography.


2011 ◽  
Vol 291-294 ◽  
pp. 1339-1343
Author(s):  
Wen Bo Zhang ◽  
Hong Rui Li ◽  
Jun Tao ◽  
Bing Bing Dong

The research in this paper optimized the extraction technique of lentinan with ultrasonic assistant method on the basis of hot water extraction technique, and investigated the promoting function of ultrasound to polysaccharides extraction. Extraction condition was selected by means of orthogonal experimental design, four factors and three levels L9(34), after key elements were respectively chosen through single factor experiments. Comparison between optimal extraction parameters of two method, hot water extraction technique and ultrasonic assistant extraction technique, showed decreased extraction temperature and significantly shortened extraction time, which existed in the second means, improved the extraction efficiency. Lentinus edodes polysaccharide extracted with ultrasonic assistant technique, the extraction rate and polysaccharide content percentage increased 6.22% and 8.66% respectively, comparative to which extracted with hot water extraction technique.


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