scholarly journals Antihypoxic activity of the VMA-10-18 derivate under hypobaric hypoxia in mice

2020 ◽  
Vol 10 (4) ◽  
pp. 29-30
Author(s):  
Anastasia Gerashchenko ◽  
Natalia Shabanova ◽  
Andrey Voronkov
Keyword(s):  
Hypobaric Hypoxia ◽  
Life Time ◽  
Test Site ◽  
Control Group ◽  
Antihypoxic Activity ◽  
Acute Hypobaric Hypoxia ◽  
Reference Drug

The present study was carried out to evaluate the effect of a new derivative VMA-10-18 (10 mg/kg) on the resistance of mice to acute hypobaric hypoxia. It was confirmed that the studied derivative contributes to an increase in the life time on the lethal test site by 2,7 times (p <0,05) compared with the control group of animals and exceeds the strength of the effect of the reference drug Metaprot by 1,2 (p <0,05).

scholarly journals The experimental study of the antihypoxic properties of harmine hydrochloride

Biomeditsina ◽  
2021 ◽  
Vol 17 (2) ◽  
pp. 71-78
Author(s):  
S. M. Adekenov ◽  
V. N. Karkischenko ◽  
M. S. Nesterov ◽  
D. A. Abaimov ◽  
A. K. Sariev
Keyword(s):  
Experimental Study ◽  
Life Expectancy ◽  
Hypobaric Hypoxia ◽  
Normobaric Hypoxia ◽  
Antihypoxic Activity ◽  
Reference Drug ◽  
Small Doses ◽  
Hypoxia With Hypercapnia ◽  
Antihypoxic Effect ◽  
Compensatory Effect

A derivative of the beta-carboline alkaloid harmine — the drug harmine hydrochloride was studied for the presence of antihypoxic properties in models of hypobaric hypoxia and normobaric hypoxia with hypercapnia. It was found that harmine hydrochloride does not have a signifi cant compensatory effect in the normobaric hypoxia test with hypercapnia. At the same time, harmine hydrochloride in small doses (2.5 and 5 mg/kg) has antihypoxic activity in the hypobaric hypoxia test, which is expressed in a statistically signifi cant increase in the life expectancy of animals treated with the drug, compared with the control, in conditions of hypoxia. According to the antihypoxic effect, harmine hydrochloride at doses of 2.5 and 5 mg/kg was found to be comparable with the reference drug (mexidol, 100 mg/kg).

scholarly journals Transcriptional profiling in the livers of rats after hypobaric hypoxia exposure

PeerJ ◽  
2019 ◽  
Vol 7 ◽  
pp. e6499 ◽  
Author(s):  
Zhenguo Xu ◽  
Zhilong Jia ◽  
Jinlong Shi ◽  
Zeyu Zhang ◽  
Xiaojian Gao ◽  
...  
Keyword(s):  
Gene Expression ◽  
High Altitude ◽  
Liver Tissue ◽  
Hypobaric Hypoxia ◽  
Cellular Response ◽  
Molecular Mechanisms ◽  
Receptor Interaction ◽  
Control Group ◽  
Acute Hypobaric Hypoxia ◽  
Genome Wide

Ascent to high altitude feels uncomfortable in part because of a decreased partial pressure of oxygen due to the decrease in barometric pressure. The molecular mechanisms causing injury in liver tissue after exposure to a hypoxic environment are widely unknown. The liver must physiologically and metabolically change to improve tolerance to altitude-induced hypoxia. Since the liver is the largest metabolic organ and regulates many physiological and metabolic processes, it plays an important part in high altitude adaptation. The cellular response to hypoxia results in changes in the gene expression profile. The present study explores these changes in a rat model. To comprehensively investigate the gene expression and physiological changes under hypobaric hypoxia, we used genome-wide transcription profiling. Little is known about the genome-wide transcriptional response to acute and chronic hypobaric hypoxia in the livers of rats. In this study, we carried out RNA-Sequencing (RNA-Seq) of liver tissue from rats in three groups, normal control rats (L), rats exposed to acute hypobaric hypoxia for 2 weeks (W2L) and rats chronically exposed to hypobaric hypoxia for 4 weeks (W4L), to explore the transcriptional profile of acute and chronic mountain sickness in a mammal under a controlled time-course. We identified 497 differentially expressed genes between the three groups. A principal component analysis revealed large differences between the acute and chronic hypobaric hypoxia groups compared with the control group. Several immune-related and metabolic pathways, such as cytokine-cytokine receptor interaction and galactose metabolism, were highly enriched in the KEGG pathway analysis. Similar results were found in the Gene Ontology analysis. Cogena analysis showed that the immune-related pathways were mainly upregulated and enriched in the acute hypobaric hypoxia group.

scholarly journals Rate of erythropoietin formation in humans in response to acute hypobaric hypoxia

1989 ◽  
Vol 66 (4) ◽  
pp. 1785-1788 ◽  
Author(s):  
K. U. Eckardt ◽  
U. Boutellier ◽  
A. Kurtz ◽  
M. Schopen ◽  
E. A. Koller ◽  
...  
Keyword(s):  
Production Rate ◽  
Half Life ◽  
Hypobaric Hypoxia ◽  
Life Time ◽  
Acute Hypobaric Hypoxia ◽  
Serum Samples ◽  
Mean Values ◽  
Altitude Exposure

This study was carried out to investigate the early changes in erythropoietin (EPO) formation in humans in response to hypoxia. Six volunteers were exposed to simulated altitudes of 3,000 and 4,000 m in a decompression chamber for 5.5 h. EPO was measured by radioimmunoassay in serum samples withdrawn every 30 min during altitude exposure and also in two subjects after termination of hypoxia (4,000 m). EPO levels during hypoxia were significantly elevated after 114 and 84 min (3,000 and 4,000 m), rising thereafter continuously for the period investigated. Mean values increased from 16.0 to 22.5 mU/ml (3,000 m) and from 16.7 to 28.0 mU/ml (4,000 m). This rise in EPO levels corresponds to 1.8-fold (3,000 m) and 3.0-fold (4,000 m) increases in the calculated production rate of the hormone. After termination of hypoxia, EPO levels continued to rise for approximately 1.5 h and after 3 h declined exponentially with an average half-life time of 5.2 h.

scholarly journals Experimental Brain Injury Induced by Acute Hypobaric Hypoxia Stimulates Changes in mRNA Expression and Stress Marker Status

2021 ◽  
Vol 8 (10) ◽  
pp. 242-247
Author(s):  
Muhammad Shoaib Tahir ◽  
Maged Almezgagi ◽  
Yu Zhang
Keyword(s):  
Oxidative Stress ◽  
Brain Injury ◽  
Mrna Expression ◽  
Hypobaric Hypoxia ◽  
Male Rats ◽  
Control Group ◽  
Dependent Manner ◽  
Acute Hypobaric Hypoxia ◽  
Time Duration ◽  
The Brain

The present study was proposed to investigate the brain injury under acute hypobaric hypoxia following alteration in mRNA expression and stress markers in a time-dependent manner. SD clean graded male rats were randomly divided into four groups for this experimental brain injury, the control group at Xining (altitude, 2270m) and hypoxia treatment groups with different time exposure day1, day2, and day3 at (altitude, 7000m) in a hypobaric chamber. After day3 exposure, the brain tissues were collected. The level of mRNA expression of VEGF and HIF1-α was assessed using qRT-PCR. The oxidative stress level of superoxide dismutase (SOD) and malondialdehyde (MDA) were determined with commercial kits. AHH with time duration significantly increased the MDA level and decreased in the activity of SOD was seen in all hypoxia treated groups as compared to the control (P< 0.001). The mRNA expression level of HIF1-α and VEGF in day1, day2, and day3 AHH groups was markedly raised when it is compared to control (P< 0.05). Ultimately, in conclusion, such results indicate that AHH stimulates oxidative stress induces brain damage in rats. Keywords: Acute hypobaric hypoxia, Brain injury, HIF-1α, Oxidative stress.

Acute Hypobaric Hypoxia (5486 m) Induces Greater Pulmonary HIF-1 Activation in Hilltop Compared to Madison Rats

2007 ◽  
Vol 8 (4) ◽  
pp. 312-321 ◽  
Author(s):  
Barbara J. Engebretsen ◽  
David Irwin ◽  
Maria E. Valdez ◽  
Mary K. O'Donovan ◽  
Alan Tucker ◽  
...  
Keyword(s):  
Hypobaric Hypoxia ◽  
Acute Hypobaric Hypoxia

Evaluation of the activity of antihypoxants with an isothiourea structure in a model of hypercapnic hypoxia with a shutdown of the cerebral hemispheres

2021 ◽  
Vol 19 (1) ◽  
pp. 55-63
Author(s):  
Vera V. Marysheva ◽  
Vladimir V. Mikheev ◽  
Petr D. Shabanov
Keyword(s):  
Acute Hypoxia ◽  
Life Time ◽  
The Body ◽  
Cerebral Hemispheres ◽  
Antihypoxic Activity ◽  
Hypoxic Episode ◽  
Hypoxia With Hypercapnia ◽  
Hypercapnic Hypoxia ◽  
Outbred Mice ◽  
The Brain

PURPOSE: To study the effect of amtizol, 2-aminobenzthiazole (2-ABT) and 2-amino-4-acetylthiazolo[5,4-b]indole (BM-606) on the resistance of male outbred mice to acute hypoxia with hypercapnia under conditions of isolated functioning of one from the hemispheres, as well as both hemispheres of the brain. METHODS: A model of acute hypoxia with hypercapnia (canned hypoxia) was used in mice of the same mass, the lifespan of all animals was determined. Temporary shutdown of the cortex of one of the hemispheres or both hemispheres was achieved by epidural application of filter paper moistened with 25% potassium chloride solution, creating a spreading depression according to Leao. Amtizol, 2-aminobenzthiazole (2-ABT) and 2-amino-4-acetylthiazolo[5,4-b]indole (BM-606) at equimolar doses of 25, 32.5, and 50 mg/kg, respectively were used as pharmacological analyzers, the compounds were injected intraperitoneally 30 min before the hypoxic episode. RESULTS: It was shown that, in contrast to amtizol, 2-ABT and VM-606 increase the life time of experimental animals when any hemisphere is turned off. The use of drugs when both hemispheres were turned off revealed that amtizol has approximately equal effect on the brain and the rest of the body, in 2-ABT antihypoxic activity is 1/3 associated with the brain, in VM-606 exclusively with the brain. CONCLUSION: The experimental model used in this work makes it possible to quite easily evaluate the effect of either one drug or compare several drugs, their role in the functioning of the cerebral hemispheres, on which part of the sample highly resistant or low resistant to hypoxia they have the greatest effect.

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