scholarly journals Peningkatan Respon Imun Non Spesik Ikan Mas (Cyprinus carpio L) Melalui Pemberian Bawang Putih (Allium Sativum)

2013 ◽  
Vol 1 (2) ◽  
Author(s):  
Kedis Lengka ◽  
Henky Manoppo ◽  
Magdalena E.F. Kolopita
Keyword(s):  
Immune Response ◽  
Cyprinus Carpio ◽  
Leucocyte Count ◽  
Allium Sativum ◽  
Total Leucocyte Count ◽  
Fish Length ◽  
Freshwater Aquaculture ◽  
Nonspecific Immune Response ◽  
Phagocytosis Activity ◽  
Different Doses

ABSTRACT This research aimed to determine the proper dose of white onion (Allium sativum) powder to stimulate the nonspecific immune response and growth of carp (Cyprinus carpio L). Fish (length 8-12 cm; weight 9.37 g in average) were obtained from BBAT Tatelu (Freshwater Aquaculture Board). After acclimatized in 1000-l fibre tank for two weeks, fish were introduced to 15 glass aquaria (80x40x40 cm) equiped with aeration at a density of 15 fish/aquarium. Fish were fed diet supplemented with white onion powder at five different doses (0, 10, 20, 30 and 40 g/kg diet), twice daily at 5%bw. Data collected included total leucocyte count (TLC), phagocytosis activity and growth of fish. Results showed that after four weeks of feeding, supplementation of white onion powder at 30 g/kg diet was effective to enhance nonspecific immune response and growth of carp. Keywords: Allium sativum, immune response, total leucocyte count, phagocytosis activity

Peningkatan Respon Kebal Non-spesifik dan Pertumbuhan Ikan Nila (Oreochromis niloticus) Melalui Pemberian Jahe, Zingiber officinale

2019 ◽  
Vol 3 (1) ◽  
Author(s):  
Clara Nunia Payung ◽  
Henky Manoppo
Keyword(s):  
Immune Response ◽  
Nile Tilapia ◽  
Leucocyte Count ◽  
Good Condition ◽  
Zingiber Officinale ◽  
Control Fish ◽  
Total Leucocyte Count ◽  
Ginger Powder ◽  
Nonspecific Immune Response ◽  
Phagocytosis Activity

The purpose of this research was to evaluate the effect ginger supplemented in food on nonspecific immune response of nile tilapia.  Juveniles with an average of weight of 27.31 g were obtained from Fish Culture and Development Board  (BP3I) Tateli and then transported to Faculty of Fisheries and Marine Science.  Fish were then cultured in 15 aquaria with a density of 15 fish per aquarium. Each aquarium was equipped with an aerator and used water recirculation system to keep the water quality still in good condition. After adaption for one weeks, fish was fed pellet supplemented with  ginger powder as treatments at five different doses including A=0, B=2,5, C=5, D=7,5, and  E=10 g/kg, each with three replications.  Fish was fed as long as four weeks at 3%/bw/d, twice daily at 08.00 am and 17.00 pm.  Data consisting of total leucocyte count and phagocytosis activity of phagocyte cells were collected at two weeks interval. Research results showed that supplementation of ginger powder into fish pellet  had significant effect on the increase of nonspecific immune response. The highest total leucocyte count and phagocytosis activity of phagocyte cells was observed on fish fed pellet supplemented with 7.5 g/kg of food and significantly different as compared to those of control fish.  As conclusion, supplementation of ginger powder into fish pellet could increase nonspecific immune response on nile tilapia. Keywords: Ginger, nonspecific immune response, total leucocytes count, phagocytosis activity, nile tilapia

scholarly journals Penggunaan ragi roti (Saccharomyces cereviciae) secara in situ untuk meningkatkan respon kebal non-spesifik ikan nila (Oreochromis niloticus)

2014 ◽  
Vol 2 (2) ◽  
Author(s):  
Meilina E Rawung ◽  
Henky Manoppo
Keyword(s):  
Immune Response ◽  
Yeast Cell ◽  
Oreochromis Niloticus ◽  
Nile Tilapia ◽  
Leucocyte Count ◽  
Yeast Cells ◽  
Total Leucocyte Count ◽  
Nile Tilapia Oreochromis Niloticus ◽  
Nonspecific Immune Response ◽  
Phagocytosis Activity

The objetive of research was to examine the effect of yeast cell Saccharomyces cereviciae on non-specific immune response of nile tilapia, Oreochromis niloticus. Juveniles weighing 31.83 g in average were cultured in five concrete tanks with a density of 30 fish per tank. The fish were fed pellet supplemented with yeast cell at five different doses (0, 10, 20, 30, 40 g/kg pellet) for three consecutive weeks as much as 4%/bb/day, twice daily. At the end of feeding, three fish from each tank were sampled to measure the immune parameters namely total leucocyte count and phagocytosis activity of phagocyte cells. Research results showed that after three weeks of feeding, total leucocyte and phagocytosis activity of fish fed pellet supplemented with yeast cells were significantly different as compared to those of control fish.  The highest total leucocyte and phagocytosis activity were achieved in fish fed pellet supplemented with 10 g of yeast cells/kg pellet.  It was concluded that the use of yeast cells for three weeks could enhance the nonspecific immune response of nile tilapia. Keywords: Saccharomyces cerreviciae, nonspecific immune response, total leucocyte count, phagocytosis activity, nile tilapia, Oreochromis niloticus

scholarly journals Evaluation of Baker’s Yeast (Saccharomyces cereviciae) In Enhancing Non Specific Immune Response and Growth of Nile Tilapia

2013 ◽  
Vol 1 (1) ◽  
Author(s):  
Usy N Manurung ◽  
Henky Manoppo ◽  
Reiny A Tumbol
Keyword(s):  
Immune Response ◽  
Nile Tilapia ◽  
Leucocyte Count ◽  
Baker’S Yeast ◽  
Control Fish ◽  
Total Leucocyte Count ◽  
Baker's Yeast ◽  
Immune Parameters ◽  
Nonspecific Immune Response ◽  
Phagocytosis Activity

This research was conducted to evaluate the efficacy of baker’s yeast in enhancing nonspecific immune response and growth of Nile tilapia. After two weeks of acclimatization in fiber tank, juveniles were put into glass aquarium at the density of 15 fish/ aquarium. Fish were fed pellet supplemented with baker’s yeast as treatment for four weeks at 5% of body weight per day, twice daily (08.00 and 17.00). Five doses of treatment used in this research were A (0 g yeast/kg pellet), B (10 g yeast/kg pellet), C (20 g yeast/kg pellet), D (30 g yeast/kg pellet), E (40 g yeast/kg pellet), Immune parameters included total leucocyte count (TLC) and phagocytosis activity were measured at the end of experiment. Fish growth was weighing at the end of research period. Anova was used to evaluate the effect of baker’s yeast on immune parameters and growth while to evaluate the different effect between treatment, Duncan test was used. Research result showed that after four week of feeding, TLC of fish fed diet supplemented with baker’s yeast significantly different compared to control fish. The highest TLC of fish was achieved in treatment B followed by treatment C. It was also found that phagocytosis activity of fish increased significantly in fish fed treatment diet with the highest PA was observed in treatment C. PA of fish in treatment C was different significantly compared to a treatment A, D and E but between treatment C and B, no significant difference was observed. Application of baker’s yeast in diet also significantly increased growth of fish (p=0,00). Fish fed diet supplemented with 20 g baker’s yeast/kg pellet has the highest weight gain compared to other fish in other treatments. It was concluded that the used of baker’s yeast at 20-30 g/kg pellet for four weeks could enhance nonspecific immune response and growth of Nile tilapia. Keywords: Saccharomyces cereviciae, total leucocyte count (TLC), Phagocytosis Activity, growth, Oreochromis niloticus

Efektivitas Ekstrak Daun Pacar Air (Impatiens balsamina L.) Untuk Meningkatkan Respon Imun Non Spesifik Ikan Nila (Oreochromis niloticus)

2019 ◽  
Vol 3 (1) ◽  
Author(s):  
Wesly Pasaribu ◽  
Sammy N.J Longdong
Keyword(s):  
Immune Response ◽  
Effective Dose ◽  
Oreochromis Niloticus ◽  
Induction Time ◽  
Nile Tilapia ◽  
Leucocyte Count ◽  
Total Leucocyte Count ◽  
Impatiens Balsamina ◽  
Four Levels ◽  
Phagocytosis Activity

The objective of this research was  to determine the most effective dose and the best induction time of  Impatiens balsamina extract in enhancing nonspesific immune response of nile tilapia (Oreochromis niloticus).  The tested fish were nile tilapia, 14-16 cm in length, and  39-42 g in weight, obtained from Balai Pengembangan dan Pembinaan Pembudidayaan Ikan (BP3I) Tateli. The  research was designed in 2x4 factorial in completely randomized design.  There were  two factors   tested  in this research, the dose  and  time.  There were four levels of dose,  A1 = 0 mg/mL extract, A2= 30 mg/mL extract, A3= 50 mg/mL extract, A4= 70 mg/mL extract; and there were  two levels of time,  B1= 7 days after injection and B2=14 days after injection.   The extract was injected intramuscularly  with a dose of  0.2 mL per fish.   Data collected in this research was the immune parameters (total leucocyte count and phagocytosis activity). The results showed that the most effective dose in enhancing nonspesific immune response was   A1=50 mg/mL extract and the best induction time was  B1= 7 day after injection.   The results also indicated  that there was significant  interaction between  dose and time in influencing  the total amount of leucocyte, but there was not  interaction in influencing the phagocytic activities. Keywords : Impatiens balsamina, immune Response, total leucocyte count, phagocytosis activity.

scholarly journals Respon kebal nonspesifik ikan mas yang diberi imunostimulan ragi roti secara oral

2017 ◽  
Vol 5 (2) ◽  
Author(s):  
Aditya P. Yanuar ◽  
Henky Manoppo
Keyword(s):  
Immune Response ◽  
Baker’S Yeast ◽  
Baker's Yeast ◽  
Immune Parameters ◽  
North Sulawesi ◽  
Freshwater Aquaculture ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Nonspecific Immune Response ◽  
Phagocytosis Activity

The effect of baker’s yeast as immunostimulant on innate immunity of carp (Cyprinus carpio L) had been investigated.  Fish was gathered from Freshwater Aquaculture Board (BBAT) Tatelu, Province of North Sulawesi.  The fish were acclimatized for a week in 15 aquariua with a density of 25 fish/aquarium. After acclimatization the fish were fed pellet supplemented with baker’s yeast as immunostimulant at 0, 5, 10, 15 and 20 g / kg of feed for four weeks as much as 5%/body weight of fish/day with the frequency of feeding twice a day at 08.00 am and 16.00 pm . This study used Completely Randomized Design (RAL) with five treatments and each treatment had three replications. Immune parameters consisting of total leucocytes and phagocytosis activity were measured in the last week. After  four weeks of feeding, the total leukocytes from the carp fed with the addition of baker’s yeast 10 g / kg of feed were significantly increased compared with the fish not being fed with the addition of bread yeast (p <0.01). Phagocytic activity of phagocyte cells from carp also increased significantly in fish fed pellet with the addition of 5 g of baker’s yeast / kg of feed (p = 0.01). In conclusion, the addition of baker’s yeast as immunostimulant in the diet at 5-10 g/kg of pellet can increase the nonspecific immune response of carp. Keywords:  baker’s yeast, carp, nonspesific immune response, total leucocytes, phagocytosis activity

scholarly journals Evaluation of The Use of Garlic (Allium sativum) in Enhancing Nonspecific Immune Response and Growth of Nile Tilapia (Oreochromis niloticus)

2013 ◽  
Vol 1 (1) ◽  
Author(s):  
Gercyana Marentek ◽  
Henky Manoppo ◽  
Sammy N.J Longdong
Keyword(s):  
Immune Response ◽  
Oreochromis Niloticus ◽  
Nile Tilapia ◽  
Allium Sativum ◽  
Control Fish ◽  
Total Leukocyte Count ◽  
Average Weight ◽  
Nile Tilapia Oreochromis Niloticus ◽  
Nonspecific Immune Response ◽  
Phagocytosis Activity

The objective of this research was to examine the effect of garlic (Allium sativum) at different doses on nonspecific immune response and growth of nile tilapia (Oreochromis niloticus). Experimental fish was nile tilapia measuring 8-15 cm with an average weight of 10.4 g per individual. Before used in the experiment, fish were reared in fiber tank for two weeks for acclimatization. After acclimatization, the fish were moved into glass aquarium (50 cm x 30 cm x 30 cm) at the density of 15 fish/aquarium. Each aquarium was equipped with aerator. Five doses of garlic as treatments used in this research consisted of A= 0 g/kg diet, B= 10 g/kg, C= 20 g/kg, D= 30 g/kg and E= 40 g/kg. Fish was fed experimental diet at a dose of 5% of body weight per day, twice a day at 08.00 and 17.00. Data collected at the end of the experiment included immune parameters (TLC and phagocytosis activity) and growth of fish. ANOVA was used to evaluate the effect of treatments on immune response and growth of fish while Duncan Test was used to evaluate the different effect between treatments. Research result showed that after four weeks of feeding, TLC of fish fed diet supplemented with garlic significantly different (p<0,01) as compared to that of control fish. The highest number of TLC was observed at treatment C (15.413x107 cell.ml-1) followed by treatment B (15.39x107 cell.ml-1), D (13,920 x 107 cell.ml-1), E (13,582 x 107 cell.ml-1) and A (12,195 x 107 cell.ml-1). TLC of fish in treatment C was different significantly compared to control as well as to other treatments. Phagocytosis activity of fish in treatment C also increased significantly (p<0,01) compared to control. The highest PA was achieved in treatment C and then treatment B, D, E and A. Growth of fish in treatment C was different significantly compared to control fish as well as other treatments. As conclusion, oral administration of garlic at 20 g/kg diet could enhance nonspecific immune response and growth of nile tilapia. Key words: Garlic, Immune response, Total leukocyte count , Phagocytosis activity, Growth

scholarly journals EFEKTIVITAS EKSTRAK DAUN CIPLUKAN (Physalis angulata L.) TERHADAP SISTEM KEKEBALAN TUBUH IKAN NILA (Oreochromis niloticus)

2018 ◽  
Vol 13 (3) ◽  
pp. 259
Author(s):  
Yusrika Octarina ◽  
Eva Prasetiyono ◽  
Dwi Febrianti ◽  
Robin Robin
Keyword(s):  
Nile Tilapia ◽  
Leucocyte Count ◽  
Positive Control ◽  
Negative Control ◽  
Physiological Solution ◽  
Total Leucocyte Count ◽  
Activity Data ◽  
Randomized Design ◽  
Physalis Angulata ◽  
Phagocytosis Activity

Penelitian ini bertujuan untuk mengetahui efektivitas ekstrak daun ciplukan (Physalis angulata) sebagai imunostimulan dalam meningkatkan jumlah leukosit dan aktivitas fagositosis pada ikan nila. Ikan nila yang digunakan berukuran panjang 10-12 cm dan bobot 70-90 g. Ikan tersebut diperoleh dari pembudidaya ikan nila di Desa Riding Panjang Kecamatan Merawang. Penelitian ini menggunakan Rancangan Acak Lengkap tunggal. Rancangan ini terdiri atas lima perlakuan (P), yaitu P1= kontrol positif (penyuntikkan dengan larutan fisiologis), P2= 4% (v/v) (1 mL ekstrak + 24 mL akuades), P3= 8% (v/v) (2 mL ekstrak + 23 mL akuades), P4= 12% (v/v) (3 mL ekstrak + 22 mL akuades) dan P5= kontrol negatif (tanpa penyuntikan). Ekstrak ciplukan diinjeksikan sebanyak 0,1 mL pada setiap ekor ikan secara intra-muskular. Indikator imun yang diamati adalah jumlah total leukosit dan aktivitas fagositosis. Data dianalisis menggunakan analisis sidik ragam (ANOVA). Hasil yang menunjukkan pengaruh antara perlakuan, selanjutnya dianalis dengan uji wilayah berganda duncan. Hasil penelitian menunjukkan bahwa ekstrak ciplukan dapat meningkatkan jumlah leukosit dan aktivitas fagositosis. Dosis terbaik dalam meningkatkan respon imun adalah dosis ekstrak P4= 12% (v/v) ekstrak dengan jumlah total leukosit (12,43 x 108 sel/mL) dan aktivitas fagositosis (46,67%).The aim of this research was to determined the effectivity of the extract Physalis angulata as immunostimulant on the amount of total leucocyte count and phagocytosis activity of nile tilapia. The tested fishes were nile tilapia with size of, 10-12 cm in total length, and 70-90 g in weight. The fish were obtained from fish farmers in Riding Panjang village Merawang Sub District. The research was designed in single completely randomized design. There were five levels of treatment (P), with P= positive control (injected with physiological solution), P2= 4% (v/v) (1 mL extract + 24 mL aquadest), P3= 8% (v/v) (2 mL extract + 23 mL aquadest), P4= 12% (v/v) (3 mL extract + 22 mL aquadest) and P5= negative control (without injection). The extract Physalis angulata L. was injected intramuscularly at a dose of 0.1 mL per fish. The immune indicators observed were total leucocyte count and phagocytosis activity). Data analyzed by using analysis of variant (Anova). if there were any significant different between the treatment, analysis continued by duncan”s multiple range test. The results of the research showed that the extract could increase the amount of total leucocyte count and phagocytosis activity. The ciplukan leaves at a dose of 12% (v/v) were the most effective dose in enhancing total leucocyte (12.43 x 108 cell/mL) and phagocytosis activity (46.67%).

Adrenergic regulation of the innate immune response in common carp (Cyprinus carpio L.)

2012 ◽  
Vol 36 (2) ◽  
pp. 306-316 ◽  
Author(s):  
Magdalena Chadzinska ◽  
Ewa Tertil ◽  
Magdalena Kepka ◽  
Trudi Hermsen ◽  
Marleen Scheer ◽  
...  
Keyword(s):  
Immune Response ◽  
Common Carp ◽  
Innate Immune Response ◽  
Cyprinus Carpio ◽  
Innate Immune ◽  
Adrenergic Regulation ◽  
Common Carp Cyprinus Carpio ◽  
Carp Cyprinus Carpio
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