scholarly journals Respon kebal nonspesifik ikan mas yang diberi imunostimulan ragi roti secara oral

2017 ◽  
Vol 5 (2) ◽  
Author(s):  
Aditya P. Yanuar ◽  
Henky Manoppo
Keyword(s):  
Immune Response ◽  
Baker’S Yeast ◽  
Baker's Yeast ◽  
Immune Parameters ◽  
North Sulawesi ◽  
Freshwater Aquaculture ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Nonspecific Immune Response ◽  
Phagocytosis Activity

The effect of baker’s yeast as immunostimulant on innate immunity of carp (Cyprinus carpio L) had been investigated.  Fish was gathered from Freshwater Aquaculture Board (BBAT) Tatelu, Province of North Sulawesi.  The fish were acclimatized for a week in 15 aquariua with a density of 25 fish/aquarium. After acclimatization the fish were fed pellet supplemented with baker’s yeast as immunostimulant at 0, 5, 10, 15 and 20 g / kg of feed for four weeks as much as 5%/body weight of fish/day with the frequency of feeding twice a day at 08.00 am and 16.00 pm . This study used Completely Randomized Design (RAL) with five treatments and each treatment had three replications. Immune parameters consisting of total leucocytes and phagocytosis activity were measured in the last week. After  four weeks of feeding, the total leukocytes from the carp fed with the addition of baker’s yeast 10 g / kg of feed were significantly increased compared with the fish not being fed with the addition of bread yeast (p <0.01). Phagocytic activity of phagocyte cells from carp also increased significantly in fish fed pellet with the addition of 5 g of baker’s yeast / kg of feed (p = 0.01). In conclusion, the addition of baker’s yeast as immunostimulant in the diet at 5-10 g/kg of pellet can increase the nonspecific immune response of carp. Keywords:  baker’s yeast, carp, nonspesific immune response, total leucocytes, phagocytosis activity

scholarly journals Evaluation of Baker’s Yeast (Saccharomyces cereviciae) In Enhancing Non Specific Immune Response and Growth of Nile Tilapia

2013 ◽  
Vol 1 (1) ◽  
Author(s):  
Usy N Manurung ◽  
Henky Manoppo ◽  
Reiny A Tumbol
Keyword(s):  
Immune Response ◽  
Nile Tilapia ◽  
Leucocyte Count ◽  
Baker’S Yeast ◽  
Control Fish ◽  
Total Leucocyte Count ◽  
Baker's Yeast ◽  
Immune Parameters ◽  
Nonspecific Immune Response ◽  
Phagocytosis Activity

This research was conducted to evaluate the efficacy of baker’s yeast in enhancing nonspecific immune response and growth of Nile tilapia. After two weeks of acclimatization in fiber tank, juveniles were put into glass aquarium at the density of 15 fish/ aquarium. Fish were fed pellet supplemented with baker’s yeast as treatment for four weeks at 5% of body weight per day, twice daily (08.00 and 17.00). Five doses of treatment used in this research were A (0 g yeast/kg pellet), B (10 g yeast/kg pellet), C (20 g yeast/kg pellet), D (30 g yeast/kg pellet), E (40 g yeast/kg pellet), Immune parameters included total leucocyte count (TLC) and phagocytosis activity were measured at the end of experiment. Fish growth was weighing at the end of research period. Anova was used to evaluate the effect of baker’s yeast on immune parameters and growth while to evaluate the different effect between treatment, Duncan test was used. Research result showed that after four week of feeding, TLC of fish fed diet supplemented with baker’s yeast significantly different compared to control fish. The highest TLC of fish was achieved in treatment B followed by treatment C. It was also found that phagocytosis activity of fish increased significantly in fish fed treatment diet with the highest PA was observed in treatment C. PA of fish in treatment C was different significantly compared to a treatment A, D and E but between treatment C and B, no significant difference was observed. Application of baker’s yeast in diet also significantly increased growth of fish (p=0,00). Fish fed diet supplemented with 20 g baker’s yeast/kg pellet has the highest weight gain compared to other fish in other treatments. It was concluded that the used of baker’s yeast at 20-30 g/kg pellet for four weeks could enhance nonspecific immune response and growth of Nile tilapia. Keywords: Saccharomyces cereviciae, total leucocyte count (TLC), Phagocytosis Activity, growth, Oreochromis niloticus

scholarly journals Kejutan suhu pada penetasan telur dan sintasan hidup larva ikan lele (Clarias gariepinus)

2015 ◽  
Vol 3 (2) ◽  
Author(s):  
Christo V.S Aer ◽  
Winda M Mingkid ◽  
Ockstan J Kalesaran
Keyword(s):  
Survival Rate ◽  
Clarias Gariepinus ◽  
Treatment Temperature ◽  
Effect Of Temperature ◽  
Temperature Shock ◽  
North Sulawesi ◽  
Freshwater Aquaculture ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
The Difference

The aim of this study was determine the effect of temperature shock on hatching capability of the catfish eggs and its larval survivals.The study was conducted at the Board of Freshwater Aquaculture (BBAT ) in Tatelu  Dimembe District, North Minahasa Regency, North Sulawesi Province. Twelve tanks were set in this experiment. Two hundred (200) eggs were put in each tank with controlled temperature of 25 C, 27 C , 29 C , and 32 ºC. Each temperature was replicated 3 times. The experimental design used in data analysis was completely randomized design (CRD).  The results showed that the treatment temperature showed significant effect on  hatching of  eggs, while the difference in temperature had no effect on the survival rate of catfish larvae.   Keywords:  Temperature shock, eggs, hatching capability, survival rate,  larvae, Clarias gariepinus.

scholarly journals PENGARUH PEMBERIAN RAGI ROTI DENGAN DOSIS YANG BERBEDA TERHADAP PERTUMBUHAN POPULASI Brachionus plicatilis

2018 ◽  
Vol 15 (2) ◽  
Author(s):  
Iksan, Muhammad Junaidi Dan Alis Mukhlis
Keyword(s):  
Population Density ◽  
Population Growth ◽  
Brachionus Plicatilis ◽  
Baker’S Yeast ◽  
Baker's Yeast ◽  
Yeast Population ◽  
Randomized Design ◽  
Key Word ◽  
Completely Randomized Design ◽  
The Difference

ABSTRAKThis study aims to determine the best dose of bakers yeast in culture Brachionus plicatilis. The research was conducted in July-September 2015 in the Mandiri Laboratory of BTN Puri Meninting West Lombok District. This experiment  uses a completely randomized design ( CRD ), which consists of 6 treatments and repeated 3 times , in order to obtain 18 units of trial that treatment A : without feed , treatment B : feed Nannochloropsis spp., treatment C : 7.5 mg/L, treatment D : 15.0 mg/L , treatment E : 22.5 mg/L , and treatment F : 30.0 mg/L. The results showed that the difference in the level of baker's yeast significantly affect the population growth of Brachionus plicatilis, which obtained the highest population density in the yeast dose of 30.0 mg/L of 90 individuals/mL Key word : bakers yeast, population growth, Brachionus plicatilisABSTRACT Penelitian ini bertujuan untuk mengetahui dosis ragi roti yang terbaik  dalam kultur Brachionus plicatilis. Penelitian dilaksanakan pada bulan Juli - September 2015  di Laboratorium Mandiri BTN Puri Meninting Kabupaten Lombok Barat. Penelitian ini menggunakan  Rancangan Acak Lengkap (RAL) yang terdiri dari 6 perlakuan dan diulang sebanyak 3 kali, sehingga diperoleh 18 unit percobaan yaitu  perlakuan  A : tanpa pakan, perlakuan  B : pakan Nannochloropsis spp.,  perlakuan C : 7,5 mg/L, perlakuan D : 15,0 mg/L, perlakuan  E :  22,5 mg/L,  dan perlakuan  F : 30,0 mg/L. Hasil penelitian menunjukan bahwa perbedaan dosis ragi roti berpengaruh nyata terhadap pertumbuhan populasi Brachionus plicatilis, dimana kepadatan populasi tertinggi diperoleh pada dosis ragi 30,0 mg/L yaitu 90 individu/mL Kata kunci : ragi roti, pertumbuhan populasi,  Brachionus plicatilis,

scholarly journals Peningkatan Respon Imun Non Spesik Ikan Mas (Cyprinus carpio L) Melalui Pemberian Bawang Putih (Allium Sativum)

2013 ◽  
Vol 1 (2) ◽  
Author(s):  
Kedis Lengka ◽  
Henky Manoppo ◽  
Magdalena E.F. Kolopita
Keyword(s):  
Immune Response ◽  
Cyprinus Carpio ◽  
Leucocyte Count ◽  
Allium Sativum ◽  
Total Leucocyte Count ◽  
Fish Length ◽  
Freshwater Aquaculture ◽  
Nonspecific Immune Response ◽  
Phagocytosis Activity ◽  
Different Doses

ABSTRACT This research aimed to determine the proper dose of white onion (Allium sativum) powder to stimulate the nonspecific immune response and growth of carp (Cyprinus carpio L). Fish (length 8-12 cm; weight 9.37 g in average) were obtained from BBAT Tatelu (Freshwater Aquaculture Board). After acclimatized in 1000-l fibre tank for two weeks, fish were introduced to 15 glass aquaria (80x40x40 cm) equiped with aeration at a density of 15 fish/aquarium. Fish were fed diet supplemented with white onion powder at five different doses (0, 10, 20, 30 and 40 g/kg diet), twice daily at 5%bw. Data collected included total leucocyte count (TLC), phagocytosis activity and growth of fish. Results showed that after four weeks of feeding, supplementation of white onion powder at 30 g/kg diet was effective to enhance nonspecific immune response and growth of carp. Keywords: Allium sativum, immune response, total leucocyte count, phagocytosis activity

scholarly journals Administrasi oral imunostimulan ragi roti (Saccharomyces cerevisiae) untuk meningkatkan pertumbuhan ikan mas (Cyprinus Carpio L.)

2017 ◽  
Vol 5 (2) ◽  
Author(s):  
Atiek P. Razak ◽  
Reni L. Kreckhoff ◽  
Juliaan Ch. Watung
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Body Weight ◽  
Cyprinus Carpio ◽  
Baker’S Yeast ◽  
Fish Growth ◽  
Yeast Cells ◽  
Fish Feed ◽  
Baker's Yeast ◽  
North Sulawesi ◽  
Freshwater Aquaculture

The research aimed to evaluate the effect of baker’s yeast as immunostimulant on the growth of carp.  Fish was obtained from Board of Freshwater Aquaculture at Tatelu, North Sulawesi Province.  Before running the experiment, the fish was acclimatized for one week in five concrete tanks measuring 2x1x1 m3 each. During acclimatization, fish was fed commercial pellet at 5%/body weight/day, twice a day.  After acclimatizationm, the fish was distributed in 15 aquaria with a density of 25 individuals per aquarium.  The research used Complete Randomized Design with five treatments namely 0, 5, 10, 15, and 20 g yeast/kg of pellet, each with three replications.  Fish was fed with treatment pellets for three weeks consecutively at a dose of 5%/body weight/day, twice a day at 08.00 am and 16.00 pm. Growth of fish was measured at the end of feeding period.  The result showed that the addition of baker’s yeast cells into fish feed significantly influenced fish growth. The best growth was achieved in fish fed pellet supplemented with 5 g yeast/kg of pellet.Keywords : Cyprinus carpio, Saccharomyces cerevisiae, immunostimulant, growth

Kejutan suhu pada penetasan telur dan sintasan hidup larva ikan lele (Clarias gariepinus)

2015 ◽  
Vol 3 (2) ◽  
Author(s):  
Christo V S Aer ◽  
Winda M Mingkid ◽  
Ockstan J Kalesaran
Keyword(s):  
Survival Rate ◽  
Clarias Gariepinus ◽  
Treatment Temperature ◽  
Effect Of Temperature ◽  
Temperature Shock ◽  
North Sulawesi ◽  
Freshwater Aquaculture ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
The Difference

The aim of this study was determine the effect of temperature shock on hatching capability of the catfish eggs and its larval survivals.The study was conducted at the Board of Freshwater Aquaculture (BBAT ) in Tatelu  Dimembe District, North Minahasa Regency, North Sulawesi Province. Twelve tanks were set in this experiment. Two hundred (200) eggs were put in each tank with controlled temperature of 25 C, 27 C , 29 C , and 32 ºC. Each temperature was replicated 3 times. The experimental design used in data analysis was completely randomized design (CRD).  The results showed that the treatment temperature showed significant effect on  hatching of  eggs, while the difference in temperature had no effect on the survival rate of catfish larvae.   Keywords:  Temperature shock, eggs, hatching capability, survival rate,  larvae, Clarias gariepinus.

scholarly journals Pengaruh Pemberian Ragi Roti dengan Dosis yang Berbeda Terhadap Pertumbuhan Populasi Brachionus Plicatilis

2016 ◽  
Vol 16 (1) ◽  
Author(s):  
Iksan, Muhammad Junaidi Dan Alis Mukhlis
Keyword(s):  
Population Density ◽  
Population Growth ◽  
Brachionus Plicatilis ◽  
Baker’S Yeast ◽  
Baker's Yeast ◽  
Yeast Population ◽  
Randomized Design ◽  
Key Word ◽  
Completely Randomized Design ◽  
The Difference

ABSTRAKPenelitian ini bertujuan untuk mengetahui dosis ragi roti yang terbaik  dalam kultur Brachionus plicatilis. Penelitian dilaksanakan pada bulan Juli - September 2015  di Laboratorium Mandiri BTN Puri Meninting Kabupaten Lombok Barat. Penelitian ini menggunakan  Rancangan Acak Lengkap (RAL) yang terdiri dari 6 perlakuan dan diulang sebanyak 3 kali, sehingga diperoleh 18 unit percobaan yaitu  perlakuan  A : tanpa pakan, perlakuan  B : pakan Nannochloropsis spp.,  perlakuan C : 7,5 mg/L, perlakuan D : 15,0 mg/L, perlakuan  E :  22,5 mg/L,  dan perlakuan  F : 30,0 mg/L. Hasil penelitian menunjukan bahwa perbedaan dosis ragi roti berpengaruh nyata terhadap pertumbuhan populasi Brachionus plicatilis, dimana kepadatan populasi tertinggi diperoleh pada dosis ragi 30,0 mg/L yaitu 90 individu/mL Kata kunci : ragi roti, pertumbuhan populasi,  Brachionus plicatilis, ABSTRACT This study aims to determine the best dose of bakers yeast in culture Brachionus plicatilis. The research was conducted in July-September 2015 in the Mandiri Laboratory of BTN Puri Meninting West Lombok District. This experiment  uses a completely randomized design ( CRD ), which consists of 6 treatments and repeated 3 times , in order to obtain 18 units of trial that treatment A : without feed , treatment B : feed Nannochloropsis spp., treatment C : 7.5 mg/L, treatment D : 15.0 mg/L , treatment E : 22.5 mg/L , and treatment F : 30.0 mg/L. The results showed that the difference in the level of baker's yeast significantly affect the population growth of Brachionus plicatilis, which obtained the highest population density in the yeast dose of 30.0 mg/L of 90 individuals/mL Key word : bakers yeast, population growth, Brachionus plicatilis

scholarly journals PENGARUH PEMBERIAN RAGI ROTI DENGAN DOSIS YANG BERBEDA TERHADAP PERTUMBUHAN POPULASI Brachionus plicatilis

2015 ◽  
Vol 15 (2) ◽  
Author(s):  
Iksan, Muhammad Junaidi Dan Alis Mukhlis
Keyword(s):  
Population Density ◽  
Population Growth ◽  
Brachionus Plicatilis ◽  
Baker’S Yeast ◽  
Baker's Yeast ◽  
Yeast Population ◽  
Randomized Design ◽  
Key Word ◽  
Completely Randomized Design ◽  
The Difference

ABSTRACT This study aims to determine the best dose of bakers yeast in culture Brachionus plicatilis. The research was conducted in July-September 2015 in the Mandiri Laboratory of BTN Puri Meninting West Lombok District. This experiment  uses a completely randomized design ( CRD ), which consists of 6 treatments and repeated 3 times , in order to obtain 18 units of trial that treatment A : without feed , treatment B : feed Nannochloropsis spp., treatment C : 7.5 mg/L, treatment D : 15.0 mg/L , treatment E : 22.5 mg/L , and treatment F : 30.0 mg/L. The results showed that the difference in the level of baker's yeast significantly affect the population growth of Brachionus plicatilis, which obtained the highest population density in the yeast dose of 30.0 mg/L of 90 individuals/mL Key word : bakers yeast, population growth, Brachionus plicatilis ABSTRAKPenelitian ini bertujuan untuk mengetahui dosis ragi roti yang terbaik  dalam kultur Brachionus plicatilis. Penelitian dilaksanakan pada bulan Juli - September 2015  di Laboratorium Mandiri BTN Puri Meninting Kabupaten Lombok Barat. Penelitian ini menggunakan  Rancangan Acak Lengkap (RAL) yang terdiri dari 6 perlakuan dan diulang sebanyak 3 kali, sehingga diperoleh 18 unit percobaan yaitu  perlakuan  A : tanpa pakan, perlakuan  B : pakan Nannochloropsis spp.,  perlakuan C : 7,5 mg/L, perlakuan D : 15,0 mg/L, perlakuan  E :  22,5 mg/L,  dan perlakuan  F : 30,0 mg/L. Hasil penelitian menunjukan bahwa perbedaan dosis ragi roti berpengaruh nyata terhadap pertumbuhan populasi Brachionus plicatilis, dimana kepadatan populasi tertinggi diperoleh pada dosis ragi 30,0 mg/L yaitu 90 individu/mL Kata kunci : ragi roti, pertumbuhan populasi,  Brachionus plicatilis, 

The use of β-glucan extracted from baker’s yeast (Saccharomyces cerevisiae) to increase non-specific immune system and resistence of tilapia (Oreochromis niloticus) to Aeromonas hydrophila

2013 ◽  
pp. 92
Author(s):  
Ida N Jamal ◽  
Reiny A Tumbol ◽  
Remy E.P Mangindaan
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Immune System ◽  
Aeromonas Hydrophila ◽  
Phagocytic Activity ◽  
Baker’S Yeast ◽  
Baker's Yeast ◽  
Yeast Saccharomyces Cerevisiae ◽  
Randomized Design ◽  
The Difference ◽  
Motile Aeromonas Septicaemia

Motile Aeromonas Septicaemia disease (MAS) attacking tilapia has increased in recent years as a consequence of intensive aquaculture activities, which led to losses in aquaculture industry. The agent causing MAS disease is Aeromonas hydrophila. The disease can be controlled with the β-glucan. As immunostimulants, β-glucans can also increase resistance in farmed tilapia. Studies on the use of β-glucan extracted from baker's yeast Saccharomyces cerevisiae was intended to evaluate the non-specific immune system of tilapia that were challenged with Aeromonas hydrophila. The method used was an experimental method with a completely randomized design consisting of four treatments with three replicats. The dose of β-glucan used as treatments were 0 mg.kg-1 fish (Control), 5 mg.kg-1 fish (B), 10 mg.kg-1 fish (C) and 20 mg.kg-1 fish (D), each treatment as injected three times at intervals of 3 days, the injection volume of 0.5 ml/fish for nine days and resistance surveillance for seven days. The results showed that the difference in the amount of β-glucan and the frequency of the injected real influence on total leukocytes, phagocytic activity and resistance. Total leukocytes, phagocytic activity and resistance to treatment was best achieved by the administration of C a dose of  10 mg.kg-1 of the fish© Penyakit Motil Aeromonas Septicaemia (MAS) yang menyerang ikan nila mengalami peningkatan selama beberapa tahun terakhir sebagai konsekuensi dari kegiatan akuakultur intensif, yang menyebabkan kerugian dalam industri budidaya. Agen utama penyebab penyakit MAS adalah Aeromonas hydrophila. Untuk mengendalikan penyakit tersebut dapat dilakukan dengan pemberian β-glukan. Sebagai imunostimulan, β-glukan juga dapat  meningkatkan resistensi pada ikan nila yang dibudidayakan. Pengkajian mengenai pemanfaatan β-glukan yang diekstrak dari ragi roti Saccharomyces cerevisiae dimaksudkan untuk menguji sistem imun non spesifik ikan nila yang diuji tantang dengan bakteri Aeromonas hydrophila. Metode yang digunakan yaitu metode eksperimen dengan rancangan acak lengkap yang terdiri dari empat perlakuan dan tiga ulangan. Dosis β-glukan  yang digunakan sebagai perlakuan sebesar 0 mg.kg-1 ikan (Kontrol), 5 mg.kg-1 ikan (B), 10 mg.kg-1 ikan (C) dan 20 mg.kg-1 ikan (D), masing-masing perlakuan diinjeksi sebanyak 3 kali dengan interval waktu 3 hari selama 9 hari, volume injeksi 0,5 mL/ekor ikan dan pengamatan resistensi selama tujuh hari. Hasil penelitian menunjukkan perbedaan jumlah β-glukan dan frekuensi pemberian yang diinjeksikan memberikan pengaruh nyata terhadap total leukosit, aktivitas fagositosis dan resistensi. Total leukosit, aktivitas fagositosis dan resistensi terbaik dicapai pada perlakuan C dengan dosis 10 mg.kg-1 ikan©

scholarly journals Kandungan Klorofil Total Daun Pada Padi Lokal Sulawesi Utara Yang Mengalami Cekaman Banjir

Jurnal MIPA ◽  
2019 ◽  
Vol 8 (2) ◽  
pp. 51
Author(s):  
Fitria Tjolleng ◽  
Parluhutan Siahaan ◽  
Nio Song Ai
Keyword(s):  
Chlorophyll Content ◽  
Physiological Responses ◽  
Time Factors ◽  
Total Chlorophyll ◽  
Total Chlorophyll Content ◽  
North Sulawesi ◽  
Randomized Design ◽  
The Media ◽  
Rice Leaves ◽  
Completely Randomized Design

Penelitian ini bertujuan untuk mengkaji respon fisiologi tanaman padi lokal Sulawesi Utara (varietas Ombong, Temo, TB, dan Sultan) terhadap cekaman banjir pada fase vegetatif berdasarkan kandungan klorofil total pada daun. Pot dan tanaman padi dimasukkan ke dalam ember sehingga terendam setinggi 27 cm di atas permukaan media. Perlakuan cekaman kebanjiran berlangsung selama 20 hari. Pengambilan data dilakukan pada hari ke-0 (sebelum perlakuan), 10, 15 dan 20 setelah perlakuan. Daun diambil sebanyak 1 g kemudian dihaluskan dan diekstraksi menggunakan alkohol 95% dan kandungan klorofil diukur dengan spektrofotometer. Data kandungan klorofil total dalam percobaan faktorial dianalisis dengan analisis sidik ragam dalam rancangan acak lengkap pada tingkat kepercayaan 95%. Hasil penelitian ini menunjukkan bahwa faktor varietas, faktor waktu dan interaksi antara keduanya tidak menunjukkan perbedaan yang nyata pada kandungan klorofil total pada daun tanaman padi (varietas Ombong, Temo, TB, dan Sultan) selama 20 hari perlakuan cekaman banjirThis study examined the physiological responses of North Sulawesi local rice plants in (Ombong, Temo, TB, and Sultan varieties) to partial submergence at the vegetative phase based on the content of total chlorophyll in the rice leaves. The pot and plants were submerged under water in a bucket about 27 cm above the surface of the media. The submergence treatment was lasted for 20 days. Data collection was carried out at day 0 (before treatment), 10, 15 and 20 after treatment. The leaves (about 1 g for ach sample) were collected, then refined and extracted using 95% ethanol. The total chlorophyll content was measured using spectrophotometer. Data of total chlorophyll content in the factorial experiment were analyzed using analysis of variance under completely randomized design at a 95% confidence level. The results of this study indicated that variety and time factors as well as their interactions showed no significant differences in the content of total chlorophyll in the leaves of rice plant varieties (Ombong, Temo, TB, and Sultan) during 20 days of partial submergence

Sign in / Sign up

Export Citation Format

Share Document