scholarly journals EFEKTIVITAS EKSTRAK DAUN CIPLUKAN (Physalis angulata L.) TERHADAP SISTEM KEKEBALAN TUBUH IKAN NILA (Oreochromis niloticus)

2018 ◽  
Vol 13 (3) ◽  
pp. 259
Author(s):  
Yusrika Octarina ◽  
Eva Prasetiyono ◽  
Dwi Febrianti ◽  
Robin Robin
Keyword(s):  
Nile Tilapia ◽  
Leucocyte Count ◽  
Positive Control ◽  
Negative Control ◽  
Physiological Solution ◽  
Total Leucocyte Count ◽  
Activity Data ◽  
Randomized Design ◽  
Physalis Angulata ◽  
Phagocytosis Activity

Penelitian ini bertujuan untuk mengetahui efektivitas ekstrak daun ciplukan (Physalis angulata) sebagai imunostimulan dalam meningkatkan jumlah leukosit dan aktivitas fagositosis pada ikan nila. Ikan nila yang digunakan berukuran panjang 10-12 cm dan bobot 70-90 g. Ikan tersebut diperoleh dari pembudidaya ikan nila di Desa Riding Panjang Kecamatan Merawang. Penelitian ini menggunakan Rancangan Acak Lengkap tunggal. Rancangan ini terdiri atas lima perlakuan (P), yaitu P1= kontrol positif (penyuntikkan dengan larutan fisiologis), P2= 4% (v/v) (1 mL ekstrak + 24 mL akuades), P3= 8% (v/v) (2 mL ekstrak + 23 mL akuades), P4= 12% (v/v) (3 mL ekstrak + 22 mL akuades) dan P5= kontrol negatif (tanpa penyuntikan). Ekstrak ciplukan diinjeksikan sebanyak 0,1 mL pada setiap ekor ikan secara intra-muskular. Indikator imun yang diamati adalah jumlah total leukosit dan aktivitas fagositosis. Data dianalisis menggunakan analisis sidik ragam (ANOVA). Hasil yang menunjukkan pengaruh antara perlakuan, selanjutnya dianalis dengan uji wilayah berganda duncan. Hasil penelitian menunjukkan bahwa ekstrak ciplukan dapat meningkatkan jumlah leukosit dan aktivitas fagositosis. Dosis terbaik dalam meningkatkan respon imun adalah dosis ekstrak P4= 12% (v/v) ekstrak dengan jumlah total leukosit (12,43 x 108 sel/mL) dan aktivitas fagositosis (46,67%).The aim of this research was to determined the effectivity of the extract Physalis angulata as immunostimulant on the amount of total leucocyte count and phagocytosis activity of nile tilapia. The tested fishes were nile tilapia with size of, 10-12 cm in total length, and 70-90 g in weight. The fish were obtained from fish farmers in Riding Panjang village Merawang Sub District. The research was designed in single completely randomized design. There were five levels of treatment (P), with P= positive control (injected with physiological solution), P2= 4% (v/v) (1 mL extract + 24 mL aquadest), P3= 8% (v/v) (2 mL extract + 23 mL aquadest), P4= 12% (v/v) (3 mL extract + 22 mL aquadest) and P5= negative control (without injection). The extract Physalis angulata L. was injected intramuscularly at a dose of 0.1 mL per fish. The immune indicators observed were total leucocyte count and phagocytosis activity). Data analyzed by using analysis of variant (Anova). if there were any significant different between the treatment, analysis continued by duncan”s multiple range test. The results of the research showed that the extract could increase the amount of total leucocyte count and phagocytosis activity. The ciplukan leaves at a dose of 12% (v/v) were the most effective dose in enhancing total leucocyte (12.43 x 108 cell/mL) and phagocytosis activity (46.67%).

Efektivitas Ekstrak Daun Pacar Air (Impatiens balsamina L.) Untuk Meningkatkan Respon Imun Non Spesifik Ikan Nila (Oreochromis niloticus)

2019 ◽  
Vol 3 (1) ◽  
Author(s):  
Wesly Pasaribu ◽  
Sammy N.J Longdong
Keyword(s):  
Immune Response ◽  
Effective Dose ◽  
Oreochromis Niloticus ◽  
Induction Time ◽  
Nile Tilapia ◽  
Leucocyte Count ◽  
Total Leucocyte Count ◽  
Impatiens Balsamina ◽  
Four Levels ◽  
Phagocytosis Activity

The objective of this research was  to determine the most effective dose and the best induction time of  Impatiens balsamina extract in enhancing nonspesific immune response of nile tilapia (Oreochromis niloticus).  The tested fish were nile tilapia, 14-16 cm in length, and  39-42 g in weight, obtained from Balai Pengembangan dan Pembinaan Pembudidayaan Ikan (BP3I) Tateli. The  research was designed in 2x4 factorial in completely randomized design.  There were  two factors   tested  in this research, the dose  and  time.  There were four levels of dose,  A1 = 0 mg/mL extract, A2= 30 mg/mL extract, A3= 50 mg/mL extract, A4= 70 mg/mL extract; and there were  two levels of time,  B1= 7 days after injection and B2=14 days after injection.   The extract was injected intramuscularly  with a dose of  0.2 mL per fish.   Data collected in this research was the immune parameters (total leucocyte count and phagocytosis activity). The results showed that the most effective dose in enhancing nonspesific immune response was   A1=50 mg/mL extract and the best induction time was  B1= 7 day after injection.   The results also indicated  that there was significant  interaction between  dose and time in influencing  the total amount of leucocyte, but there was not  interaction in influencing the phagocytic activities. Keywords : Impatiens balsamina, immune Response, total leucocyte count, phagocytosis activity.

Peningkatan Respon Kebal Non-spesifik dan Pertumbuhan Ikan Nila (Oreochromis niloticus) Melalui Pemberian Jahe, Zingiber officinale

2019 ◽  
Vol 3 (1) ◽  
Author(s):  
Clara Nunia Payung ◽  
Henky Manoppo
Keyword(s):  
Immune Response ◽  
Nile Tilapia ◽  
Leucocyte Count ◽  
Good Condition ◽  
Zingiber Officinale ◽  
Control Fish ◽  
Total Leucocyte Count ◽  
Ginger Powder ◽  
Nonspecific Immune Response ◽  
Phagocytosis Activity

The purpose of this research was to evaluate the effect ginger supplemented in food on nonspecific immune response of nile tilapia.  Juveniles with an average of weight of 27.31 g were obtained from Fish Culture and Development Board  (BP3I) Tateli and then transported to Faculty of Fisheries and Marine Science.  Fish were then cultured in 15 aquaria with a density of 15 fish per aquarium. Each aquarium was equipped with an aerator and used water recirculation system to keep the water quality still in good condition. After adaption for one weeks, fish was fed pellet supplemented with  ginger powder as treatments at five different doses including A=0, B=2,5, C=5, D=7,5, and  E=10 g/kg, each with three replications.  Fish was fed as long as four weeks at 3%/bw/d, twice daily at 08.00 am and 17.00 pm.  Data consisting of total leucocyte count and phagocytosis activity of phagocyte cells were collected at two weeks interval. Research results showed that supplementation of ginger powder into fish pellet  had significant effect on the increase of nonspecific immune response. The highest total leucocyte count and phagocytosis activity of phagocyte cells was observed on fish fed pellet supplemented with 7.5 g/kg of food and significantly different as compared to those of control fish.  As conclusion, supplementation of ginger powder into fish pellet could increase nonspecific immune response on nile tilapia. Keywords: Ginger, nonspecific immune response, total leucocytes count, phagocytosis activity, nile tilapia

scholarly journals Penggunaan ragi roti (Saccharomyces cereviciae) secara in situ untuk meningkatkan respon kebal non-spesifik ikan nila (Oreochromis niloticus)

2014 ◽  
Vol 2 (2) ◽  
Author(s):  
Meilina E Rawung ◽  
Henky Manoppo
Keyword(s):  
Immune Response ◽  
Yeast Cell ◽  
Oreochromis Niloticus ◽  
Nile Tilapia ◽  
Leucocyte Count ◽  
Yeast Cells ◽  
Total Leucocyte Count ◽  
Nile Tilapia Oreochromis Niloticus ◽  
Nonspecific Immune Response ◽  
Phagocytosis Activity

The objetive of research was to examine the effect of yeast cell Saccharomyces cereviciae on non-specific immune response of nile tilapia, Oreochromis niloticus. Juveniles weighing 31.83 g in average were cultured in five concrete tanks with a density of 30 fish per tank. The fish were fed pellet supplemented with yeast cell at five different doses (0, 10, 20, 30, 40 g/kg pellet) for three consecutive weeks as much as 4%/bb/day, twice daily. At the end of feeding, three fish from each tank were sampled to measure the immune parameters namely total leucocyte count and phagocytosis activity of phagocyte cells. Research results showed that after three weeks of feeding, total leucocyte and phagocytosis activity of fish fed pellet supplemented with yeast cells were significantly different as compared to those of control fish.  The highest total leucocyte and phagocytosis activity were achieved in fish fed pellet supplemented with 10 g of yeast cells/kg pellet.  It was concluded that the use of yeast cells for three weeks could enhance the nonspecific immune response of nile tilapia. Keywords: Saccharomyces cerreviciae, nonspecific immune response, total leucocyte count, phagocytosis activity, nile tilapia, Oreochromis niloticus

scholarly journals MASCULINIZATION OF NILE TILAPIA (Oreochromis niloticus) USING JAVA LONG PEPPER (Piper retrofractum) EXTRACT THROUGH LARVAL IMMERSION

2019 ◽  
Vol 4 (2) ◽  
pp. 29-34
Author(s):  
Hafizhotur Rohmaniah ◽  
Denny Syaputra ◽  
Ahmad Fahrul Syarif
Keyword(s):  
Growth Rate ◽  
Survival Rate ◽  
Nile Tilapia ◽  
Positive Control ◽  
Negative Control ◽  
Absolute Weight ◽  
Length Growth ◽  
Weight Growth ◽  
Randomized Design ◽  
Absolute Length

The aimed of this study was to evaluate the effectiveness of java long pepper extract in the increasing of the percentage of males nile tilapia. The design of experiment was completely randomized design with 5 treatments i.e : A (negative control), B (1,99 mg L-1), C (3,99 mg L-1), D (5,99 mg L-1) and positive control (20 mg L-1 17α-metiltestosteron) with three replicates per level of treatment. Immersion of 10-day-old larvae for 24 hours with 50 larvae in 10 L medium of treatments and cultivated for 60 days and identification of gender using the acetocarmin method. The results showed that java long pepper extract was effective to increase the percentage of males with dose of 1,99 mg L-1 (81,78±6,81%), 3,99 mg L-1 (79,00±2,99%) and 5,99 mg L-1 (87,42±4,85%) while the negative control 39,23±7,74%. Java long pepper extract dose of 5,99 mg L-1 were not significantly different with the treatment of 17α-metiltestosteron i.e : 91,71±3,93%. Percentage of survival rate 47.33±3.06% to 53.33±8.08%, specific growth rate 3,14±0,15% to 3,40±0,08%, absolute weight growth 1.97±0.59 g to 3.07±0.38 g and absolute length growth of 3.73±0.55 cm to 3.95±0.16 cm. Java long pepper extract gives a significant effects to the spesific growth  rate and absolute weight growth. Treatment A, B, C, D and E had no effect on survival rate and the absolute length growth of nile tilapia during 60 days cultivation period.

scholarly journals Pertumbuhan tiga jenis ikan dan kangkung darat (Ipomoea reptans Poir) yang dipelihara dengan sistem akuaponik

2018 ◽  
Vol 17 (2) ◽  
pp. 175 ◽  
Author(s):  
Zahidah Hasan ◽  
Y. Andriani ◽  
Y. Dhahiyat ◽  
A. Sahidin ◽  
M. R. Rubiansyah
Keyword(s):  
Nile Tilapia ◽  
Positive Control ◽  
Negative Control ◽  
Fish Growth ◽  
Fertilizer Treatment ◽  
African Catfish ◽  
North African ◽  
Water Spinach ◽  
Randomized Design ◽  
Control Water

This study aims to determine the growth of fish (Nile tilapia, North African catfish, and goldfish) which cultured along with water spinach in aquaponics system. The study was conducted at the Laboratory of Aquaculture, Ciparanje, Facul-ty of Fisheries and Marine Sciences, Padjadjaran University from February to April 2016. The experiment was designed using a completely randomized design with 5 treatments and 3 replicates. Treatment A: positive control, water spinach grown on soil and compost fertilizer, Treatment B: negative control, water spinach grown on soil without compost fertilizer, treatment C: aquaponics Nile tilapia and water spinach, Treatment D: aquaponics North African catfish and water spinach, and treatment E: aquaponics goldfish and water spinach. The measured parameters in this study were fish growth and water spinach growth include stem height, number of leaves, biomass as well as water quality included nitrates, ammonia and phosphates. The results showed that the highest fish growth, obtained in combination of catfish and water spinach with growth of 7.8±1.18 grams and the highest water spinach growth obtain in koi cultured with water spinach with average biomass 30.40±5.59 grams. AbstrakPenelitian ini bertujuan untuk menentukan pertumbuhan tiga jenis ikan (lele, nila, dan koi) yang dipelihara bersama de-ngan kangkung darat dalam sistem akuaponik. Penelitian dilakukan di Laboratorium Budi daya Perikanan, Ciparanje, Fakultas Perikanan dan Ilmu Kelautan Universitas Padjadjaran dari bulan Februari hingga April 2016. Metode yang digunakan dalam penelitian ini adalah metode eksperimental menggunakan rancangan acak lengkap dengan lima perla-kuan dan diulang empat kali. Perlakuan A: kontrol positif, tanaman kangkung darat ditanam menggunakan tanah dan pupuk kompos; perlakuan B: kontrol negatif, tanaman kangkung darat ditanam menggunakan tanah tanpa menggunakan pupuk kompos; perlakuan C: akuaponik dengan ikan nila dan tanaman kangkung darat; perlakuan D: akuaponik dengan ikan lele dan tanaman kangkung darat, dan perlakuan E: ikan koi dan tanaman kangkung darat. Parameter yang diukur dalam penelitian ini adalah pertumbuhan bobot ikan, dan pertumbuhan kangkung yang meliputi tinggi batang, jumlah daun, dan biomassa panen serta kualitas air yang mencakup nitrat, ammonia, dan fosfat. Hasil penelitian menunjukkan bahwa pertumbuhan bobot ikan tertinggi diperoleh pada kombinasi lele dan kangkung darat dengan pertumbuhan sebe-sar 7,8±1,18 g dan pertumbuhan tanaman kangkung tertinggi diperoleh pada pemeliharaan bersama koi yang mengha-silkan biomassa sebesar 30,40±5,59 g.

scholarly journals Combination of Moringa Leaf Meal and Probiotics in Feed for Tilapia (Oreochromis niloticus) Seeds Survival and Immune System

2021 ◽  
Vol 10 (1) ◽  
pp. 117
Author(s):  
Fariq Azhar ◽  
Nanang Satria Sumarjan ◽  
Siti Hilyana
Keyword(s):  
Intestinal Bacteria ◽  
Positive Control ◽  
Negative Control ◽  
Physiological Solution ◽  
Leaf Meal ◽  
Defense Systems ◽  
Injection Treatment ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Control Injection

Prevention of disease in tilapia seeds can be done by increasing non-specific defense systems by improving and supplementing the nutritional content of the feed. This study aims to determine the effect of adding Moringa leaf meal combined with probiotics to feed on the survival rate of tilapia seeds and the number of seed blood cells that have been infected with Aeromonas hydrophila. The method used was an experimental method using a Completely Randomized Design (CRD) with 5 treatments and 3 replications. Treatment A = addition of 4% moringa leaf meal + 6 ml / kg of probiotic feed + A. hydrophila injection; Treatment B = addition of 6% moringa leaf meal + 6 ml / kg of probiotic feed + A. hydrophila injection; Treatment C = addition of 8% moringa leaf meal + 6 ml / kg of probiotic feed + A. hydrophila injection; Treatment D = feed + probiotic 6 ml / kg of feed (positive control) + injection of A. hydrophila; Treatment E = feed + probiotic 6 ml / kg of feed (negative control) + injection of physiological solution. The results showed the addition of Moringa leaf meal combined with probiotics got the best results in treatment C with a survival value of 86.67%, hemoglobin levels of 2.3 g%, erythrocytes of 2.20 × 106 cells, leukocytes of 11.5 × 104 cells, and the total number of intestinal bacteria was 10.34 × 106 cfu / ml.

scholarly journals Evaluation of Baker’s Yeast (Saccharomyces cereviciae) In Enhancing Non Specific Immune Response and Growth of Nile Tilapia

2013 ◽  
Vol 1 (1) ◽  
Author(s):  
Usy N Manurung ◽  
Henky Manoppo ◽  
Reiny A Tumbol
Keyword(s):  
Immune Response ◽  
Nile Tilapia ◽  
Leucocyte Count ◽  
Baker’S Yeast ◽  
Control Fish ◽  
Total Leucocyte Count ◽  
Baker's Yeast ◽  
Immune Parameters ◽  
Nonspecific Immune Response ◽  
Phagocytosis Activity

This research was conducted to evaluate the efficacy of baker’s yeast in enhancing nonspecific immune response and growth of Nile tilapia. After two weeks of acclimatization in fiber tank, juveniles were put into glass aquarium at the density of 15 fish/ aquarium. Fish were fed pellet supplemented with baker’s yeast as treatment for four weeks at 5% of body weight per day, twice daily (08.00 and 17.00). Five doses of treatment used in this research were A (0 g yeast/kg pellet), B (10 g yeast/kg pellet), C (20 g yeast/kg pellet), D (30 g yeast/kg pellet), E (40 g yeast/kg pellet), Immune parameters included total leucocyte count (TLC) and phagocytosis activity were measured at the end of experiment. Fish growth was weighing at the end of research period. Anova was used to evaluate the effect of baker’s yeast on immune parameters and growth while to evaluate the different effect between treatment, Duncan test was used. Research result showed that after four week of feeding, TLC of fish fed diet supplemented with baker’s yeast significantly different compared to control fish. The highest TLC of fish was achieved in treatment B followed by treatment C. It was also found that phagocytosis activity of fish increased significantly in fish fed treatment diet with the highest PA was observed in treatment C. PA of fish in treatment C was different significantly compared to a treatment A, D and E but between treatment C and B, no significant difference was observed. Application of baker’s yeast in diet also significantly increased growth of fish (p=0,00). Fish fed diet supplemented with 20 g baker’s yeast/kg pellet has the highest weight gain compared to other fish in other treatments. It was concluded that the used of baker’s yeast at 20-30 g/kg pellet for four weeks could enhance nonspecific immune response and growth of Nile tilapia. Keywords: Saccharomyces cereviciae, total leucocyte count (TLC), Phagocytosis Activity, growth, Oreochromis niloticus

scholarly journals Efek Antimitogenik Fraksi Alkaloid Achyranthes aspera Linn. terhadap Induksi Apoptosis pada Mencit yang Terinfeksi Mycobacterium tuberculosis

2016 ◽  
Vol 3 (1) ◽  
pp. 8-15
Author(s):  
Dewa Ketut Meles ◽  
. Wurlina ◽  
I Dewa Putu Anom Adnyana ◽  
Sunarni Zakaria ◽  
Dewa Made Sucipta Putra ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Leucocyte Count ◽  
Apoptotic Cell ◽  
Positive Control ◽  
Negative Control ◽  
Total Leucocyte Count ◽  
Treatment Groups ◽  
Achyranthes Aspera ◽  
Alkaloid Fraction ◽  
Apoptotic Induction

Tujuan dari penelitian ini adalah untuk mendeteksi efek antimitogenik dari ekstrak fraksi alkaloid daun Achyranthes aspera Linn. terhadap induksi apoptosis, pertumbuhan dan perkembangan sel paru-paru yang diinfeksi oleh Mycobacterium tuberculosis. Efek antimitogenic dari alkaloid A. aspera diujikan pada 120 ekor mencit yang diinfeksikan dengan 100 sel/mL M. tuberculosis dan dibagi menjadi 6 kelompok sehingga masing-masing kelompok terdiri dari 20 ekor. Kelompok perlakuan terdiri dari: kontrol negatif dengan tikus sehat yang diberikan adjuvant saja, kontrol positif dengan diberikan rifampisin 600 mg/kgbb/hari, dan kelompok perlakuan P0 , P1 , P2 , dan P3 yang diinfeksi M. tuberculosis dan diberikan alkaloid dengan dosis bertingkat 0, 60, 12, dan 180 mg/kgbb/po/hari selama 30 hari. Parameter pengamatan terdiri dari: jumlah total leukosit, jumlah total jenis leukosit, jumlah karbuncel di paru-paru, dan jumlah sel paru-paru yang mengalami apoptosis. Hasil penelitian menunjukkan pada tikus yang terinfeksi M. tuberculosis mengalami penurunan jumlah total dan jenis leukosit (eosinofil, neutrofil, lmfosit dan monosit), dan jumlah karbunkel di paru-paru pada kelompok perlakuan akaloid mulai dosis 60 mg/kgbb. Jumlah sel di paru-paru yang mengalami apoptosis juga mengalami penurunan pada kelompok pemberian akaloid daun A. aspera mulai dosis 60 mg/kgBB sama dengan kelompok rifampisin. Kesimpulan dari penelitian bahwa alkaloid daun A. aspera menyebabkan penurunan jumlah total dan jenis leukosit, serta jumlah karbuncel dan sel paru yang mengalami apoptosis pada tikus yang terinfeksi oleh M. tuberculosis.Kata kunci: Achyranthes aspera Linn., antimitogenik, paru-paru tuberkulosis, apoptotis, leukosit (The Antimitogenic Effect of Alkaloid Fraction of Achyranthes aspera Linn. on Apoptotic Induction in Mice Infected by Mycobacterium tuberculosis)The aims of this research was to determine the antimitogenic effect of alkaloid Achyranthes aspera Linn. on apoptotic induction, growth and cell development in lung cell infected by Mycobacterium tuberculosis. Antimitogenic effect of alkaloid A. aspera was tested in 120 mice that infected with 100 cell/mL M. tuberculosis and divided into 6 groups so that each group consist of 20 mice. The treatment groups were: negative control that healthy mice was given adjuvans only, positive control that was given rifampisin 600 mg/kg bb/day, and treatment group of P0 , P1 , P2 and P3 infected by M. tuberculosis and given alkaloid with dose 0, 60, 12 and 180 mg/kgbw/po/day continously during 30 days. The parameter of observation were total leucocyte count, total differential leucocyte count, total carbuncel in lung, and percentage of apoptotic lung cells. The result showed that mice infected by M. tuberculosis have decreased in total leucocyte and diferentiated leucocyte total (eosinophil, neutrophil, lymphocyte, and monocyte), and total carbuncel in lungs after treated by akaloid A. aspera with dose begin 60 mg/kgbw. Apoptotic cell in lung was also decreased in the group tretaed by akaloid A. aspera with dose begin 60 mg/kgbw that the value was equal to the group of rifampisin. In conclusion, treatment of alkaloid from A. aspera caused depreciation in leucocyte total and leucocyte differentiation, and total of carbuncel and apoptotic cell in the lung in mouse that infected by M. tuberkulosis.Keywords: Achyranthes aspera Linn., antimitogenic, tuberculosis lung, apoptotic, leucocyte 

scholarly journals EFEK PENINGKATAN SUHU TERHADAP JUMLAH LEUKOSIT IKAN NILA (Oreochromis niloticus) (The Effect of Temperature Increase on Leukocyte Count of Nile Tilapia (Oreochromis niloticus))

2016 ◽  
Vol 10 (1) ◽  
pp. 31
Author(s):  
Nauval Gibran Lubis ◽  
Sugito Sugito ◽  
Zuhrawati Zuhrawati ◽  
Zuraidawati Zuraidawati ◽  
Nuzul Asmilia ◽  
...  
Keyword(s):  
Water Temperature ◽  
Oreochromis Niloticus ◽  
Temperature Increase ◽  
Nile Tilapia ◽  
Leucocyte Count ◽  
Effect Of Temperature ◽  
Total Leucocyte Count ◽  
Randomized Design ◽  
Nile Tilapia Oreochromis Niloticus ◽  
Range Test

The aim of this research was to find out the effect of water temperature increase on leucocyte count of nile tilapia. The blood sample from 9 tilapia with weigh of 40-50 g were used in this study. The research was conducted using completely randomized design (CRD) with 3 treatments and 3 replications. Treatment P1 as a control with aquarium temperature was maintained at 29±1 C. Water temperature in treatment P2 and P3 was maintained at 32±1 C and 35±1 C respectively. The aquarium size in each treatment group was 80x60x40 cm with height of water 30 cm. The treatment was done for 15 days and on day 16th, the blood were collected from caudal vein. Data were analyzed with analysis of variance (Anova) and followed with Duncan's multiple range test. The result showed that total leucocyte count (x 105/mm3) of nile tilapia in group P1, P2, and P3 were 3.08±0.88, 1.70±0.52, and 1.69±0.20 respectively. lts showed that an increase in temperature significantly affect (P<0.05) the leucocyte count of nile tilapia. The conclusion of this research is the increase in water temperature in aquarium decrease the leucocyte count of nile tilapia.

Aktivitas Antifungi Air Perasan Bawang Merah (Allium ascalonicum L.) Terhadap Candida albicans Secara In Vitro

2017 ◽  
Vol 9 (2) ◽  
pp. 71
Author(s):  
Nurhasanah Nurhasanah ◽  
Fauzia Andrini ◽  
Yulis Hamidy
Keyword(s):  
Candida Albicans ◽  
Antifungal Activity ◽  
Allium Sativum ◽  
Fungicidal Activity ◽  
Positive Control ◽  
Negative Control ◽  
Randomized Design ◽  
Significant Difference ◽  
Completely Randomized Design

Shallot (Allium ascalonicum L.) has been known as traditional medicine. Shallot which has same genus with garlic(Allium sativum L.) contains allicin that is also found in garlic and has been suspected has fungicidal activity toCandida albicans. It is supported by several researches. Therefore, shallot is suspected has antifungal activity too.The aim of this research was to know antifungal activity of shallot’s water extortion againsts Candida albicans invitro. This was a laboratory experimental research which used completely randomized design, with diffusion method.Shallot’s water extortion was devided into three concentrations, there were 50%, 100% and 200%. Ketoconazole 2%was positive control and aquadest was negative control. The result of this research based on analysis of varians(Anova), there was significant difference between several treatments and was confirmed with Duncan New MultipleRange Test (DNMRT) p<0,05, there was significant difference between 100% shallot’s water extortion with othertreatments, but there was no significant difference between 50% shallot’s water extortion with 200% shallot’s. Theconclusion was shallot’s water extortion had antifungal activity againsts Candida albicans with the best concentration100%, but it was lower than ketoconazole 2%.

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